个人简介

1992 PhD Doctor of Philosophy University of Reading Biochemical Engineering 1989 BSc Hons Bachelor of Science (Honours) University of Reading Biotechnology

研究领域

Gary Lye leads the UCL Bioconversion-Chemistry-Engineering Interface (BiCE) Programme. This involves a multidisciplinary group of researchers interested in novel approaches to speed the development of the next generation of complex pharmaceuticals and methods to aid the uptake and integration of biocatalysis in pharmaceutical syntheses. The programme brings together a group of researchers from three UCL faculties with a long history of collaboration including Professor John Ward (Structural and Molecular Biology) and Dr Helen Hailes (Chemistry) together with Dr Frank Baganz, Dr Paul Dalby, Dr Martina Micheletti and Dr Nicolas Szita (Biochemical Engineering). It is supported by a group of 13 leading national and international companies who comprise the BiCE Industrial Steering Group. A major focus of Gary's work is the creation of novel automated micro biochemical approaches to speed bioprocess design and optimisation. This has been facilitated by a recent £4.5M investment in the UCL Centre for Micro Biochemical Engineering which he manages. The research focuses on the detailed engineering characterisation of automated microwell systems in order to enable accurate predictions of large scale process performance. These predictions can then be verified in the department\'s large scale bioprocessing facilities. Related studies have addressed miniature stirred bioreactor technologies which have now been commercialised. In collaboration with BiCE colleagues these micro biochemical engineering approaches are being applied to the evaluation of both evolved and metabolically engineered biocatalysts and integrated chemo-enzymatic syntheses. Studies as part of the IMRC in Bioprocessing and in collaboration with the UK Health Protection Agency (Porton Down) have helped established the manufacture of a new meningococcal vaccine which is now in clinical trials. Most recently collaboration with Dr Chris Mason and Dr Farlan Veraitch within the RegenMed programme has led to studies on the automated expansion and differentiation of stem cells for use in drug screening and human cell therapy. Funding for the above research has come from the UK EPSRC, the TSB Technology Programme and a range of company collaborators.

Bioprocessing Environment

近期论文

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Immobilisation and kinetics of monoamine oxidase (MAO-N-D5) enzyme in polyvinyl alcohol gels Journal article; 2016; JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC Links: IRIS | Author's | DOI Authors: Markosova K,Dolejs I,Stloukal R,Rios-Solis L,Rosenberg M,Micheletti M,Lye GJ,Turner NJ,Rebros M click to collapse Impact of cofactor-binding loop mutations on thermotolerance and activity of E-coli transketolase Journal article; 2016; ENZYME AND MICROBIAL TECHNOLOGY Links: IRIS | UCL Discovery | Author's | DOI Authors: Morris P,Rios-Solis L,Garcia-Arrazola R,Lye GJ,Dalby PA click to collapse Selective fractionation of Sugar Beet Pulp for release of fermentation and chemical feedstocks; optimisation of thermo-chemical pre-treatment Journal article; 2016; BIORESOURCE TECHNOLOGY Links: IRIS | UCL Discovery | Author's | DOI Authors: Hamley-Bennett C,Lye GJ,Leak DJ click to collapse Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose Journal article; 2016; GREEN CHEMISTRY Links: IRIS | UCL Discovery | Author's | DOI Authors: Subrizi F,Cardenas-Fernandez M,Lye GJ,Ward JM,Dalby PA,Sheppard TD,Hailes HC click to collapse Ultra scale-down approaches to enhance the creation of bioprocesses at scale: impacts of process shear stress and early recovery stages Journal article; 2016; CURRENT OPINION IN CHEMICAL ENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: Rayat ACME,Chatel A,Hoare M,Lye GJ click to collapse Centrifugal partition chromatography in a biorefinery context: Separation of monosaccharides from hydrolysed sugar beet pulp Journal article; 2015; JOURNAL OF CHROMATOGRAPHY A Links: IRIS | UCL Discovery | Author's | DOI Authors: Ward DP,Cardenas-Fernandez M,Hewitson P,Ignatova S,Lye GJ click to collapse Creation of an ultra scale-down bioreactor mimic for rapid development of lignocellulosic enzymatic hydrolysis processes Journal article; 2015; JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY Links: IRIS | UCL Discovery | Author's | DOI Authors: Conroy N,Tebble I,Lye GJ click to collapse Design and parallelisation of a miniature photobioreactor platform for microalgal culture evaluation and optimisation Journal article; 2015; BIOCHEMICAL ENGINEERING JOURNAL Links: IRIS | UCL Discovery | Author's | DOI Authors: Ojo EO,Auta H,Baganz F,Lye GJ click to collapse DEVELOPMENT OF pH STABLE MEDIA FOR PHOTOTROPHIC AND HETEROTROPHIC CULTIVATION OF CHLORELLA SOROKINIANA Journal article; 2015; EUROPEAN JOURNAL OF PHYCOLOGY Links: IRIS | Author's Authors: Ojo EO,Auta H,Baganz F,Lye GJ click to collapse Evaluation of ionic liquids as dispersed phase during the production of lactones with E-coli in a three phase partitioning bioreactor Journal article; 2015; CHEMICAL ENGINEERING JOURNAL Links: IRIS | Author's | DOI Authors: Melgarejo-Torres R,Castillo-Araiza CO,Lopez-Ordaz P,Calleja-Castaneda NV,Cano-Velasco JL,Camacho-Ruiz RM,Lye GJ,Huerta-Ochoa S click to collapse High throughput screening of monoamine oxidase (MAO-N-D5) substrate selectivity and rapid kinetic model generation Journal article; 2015; JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC Links: IRIS | UCL Discovery | Author's | DOI Authors: Rios-Solis L,Mothia B,Yi S,Zhou Y,Micheletti M,Lye GJ click to collapse Mathematical model of a three phase partitioning bioreactor for conversion of ketones using whole cells Journal article; 2015; CHEMICAL ENGINEERING JOURNAL Links: IRIS | UCL Discovery | Author's | DOI Authors: Melgarejo-Torres R,Castillo-Araiza CO,Dutta A,Beny G,Torres-Martinez D,Gutierrez-Rojas M,Lye GJ,Huerta-Ochoa S click to collapse Modelling and optimisation of the one-pot, multi-enzymatic synthesis of chiral amino-alcohols based on microscale kinetic parameter determination Journal article; 2015; CHEMICAL ENGINEERING SCIENCE Links: IRIS | UCL Discovery | Author's | DOI Authors: Rios-Solis L,Morris P,Grant C,Odeleye AOO,Hailes HC,Ward JM,Dalby PA,Baganz F,Lye GJ click to collapse The protein fraction from wheat-based dried distiller's grain with solubles (DDGS): extraction and valorization Journal article; 2015; NEW BIOTECHNOLOGY Links: IRIS | UCL Discovery | Author's | DOI Authors: Villegas-Torres MF,Ward JM,Lye GJ click to collapse A novel microscale crossflow device for the rapid evaluation of microfiltration processes Journal article; 2014; Journal of Membrane Science Links: IRIS | UCL Discovery | DOI Authors: Rayat ACME,Lye GJ,Micheletti M click to collapse Engineering characterisation of a shaken, single-use photobioreactor for early stage microalgae cultivation using Chlorella sorokiniana. Journal article; 2014; Bioresour Technol Links: IRIS | UCL Discovery | Author's | DOI Authors: Ojo EO,Auta H,Baganz F,Lye GJ click to collapse Evaluation of CV2025 omega-transaminase for the bioconversion of lignin breakdown products into value-added chemicals: synthesis of vanillylamine from vanillin Journal article; 2014; BIOCATALYSIS AND BIOTRANSFORMATION Links: IRIS | UCL Discovery | Author's | DOI Authors: Du CJ,Rios-Solis L,Ward JM,Dalby PA,Lye GJ click to collapse Impact of aeration strategies on fed-batch cell culture kinetics in a single-use 24-well miniature bioreactor Journal article; 2014; BIOCHEMICAL ENGINEERING JOURNAL Links: IRIS | UCL Discovery | Author's | DOI Authors: Betts JPJ,Warr SRC,Finka GB,Uden M,Town M,Janda JM,Baganz F,Lye GJ click to collapse Kinetic mathematical model for ketone bioconversion using Escherichia coli TOP10 pQR239 Journal article; 2014; CHEMICAL ENGINEERING JOURNAL Links: IRIS | UCL Discovery | Author's | DOI Authors: Melgarejo-Torres R,Castillo-Araiza O,Lopez-Ordaz P,Torres-Martinez D,Gutierrez-Rojas M,Lye GJ,Huerta-Ochoa S click to collapse Microscale methods to rapidly evaluate bioprocess options for increasing bioconversion yields: application to the ?-transaminase synthesis of chiral amines. Journal article; 2014; Bioprocess Biosyst Eng Links: IRIS | UCL Discovery | Author's | DOI Authors: Halim M,Rios-Solis L,Micheletti M,Ward JM,Lye GJ click to collapse On the fluid dynamics of a laboratory scale single-use stirred bioreactor. Journal article; 2014; Chem Eng Sci Links: IRIS | UCL Discovery | Author's | DOI Authors: Odeleye AO,Marsh DT,Osborne MD,Lye GJ,Micheletti M click to collapse Biochemical engineering characterisation of a shaken micro-photobioreactor platform for high-throughput development of microalgae cultivation processes Conference ; 2013; AIChE 2013 - 2013 AIChE Spring Meeting and 9th Global Congress on Process Safety, Conference Proceedings Links: IRIS Authors: Ojo E,Auta H,Miller K,Baganz F,Lye GJ click to collapse Characterization of lentiviral vector production using microwell suspension cultures of HEK293T-derived producer cells. Journal article; 2013; Hum Gene Ther Methods Links: IRIS | UCL Discovery | Author's | DOI Authors: Guy HM,McCloskey L,Lye GJ,Mitrophanous KA,Mukhopadhyay TK click to collapse ENGINEERING CHARACTERIZATION OF A SHAKEN MICRO-BIOREACTOR PLATFORM FOR PARALLEL EVALUATION OF MICROALGAE CULTIVATION AND SCALE-UP Journal article; 2013; PHYCOLOGIA Links: IRIS | UCL Discovery | Author's Authors: Ojo EO,Auta H,Baganz F,Lye GJ click to collapse Microfluidic multi-input reactor for biocatalytic synthesis using transketolase Journal article; 2013; Journal of Molecular Catalysis B: Enzymatic Links: IRIS | UCL Discovery | DOI Authors: Lawrence J,O'Sullivan B,Lye GJ,Wohlgemuth R,Szita N click to collapse