Keshavarz-Moore, Eli - University College London - Department of Biochemical Engineering

个人简介

Reader, Department of Biochemical Engineering, UCL (2006-present) Senior Lecturer, Department of Biochemical Engineering, UCL (1998-2006) Lecturer, Biochemical Engineering, UCL (1990-1998) Honorary Lecturer, Department of Chemical and Biochemical Engineering,UCL (1988-1990) Consultant, Coopers & Lybrand Associates Limited, U.K. (1979-1983) Analyst, ICL Dataskil , U.K. (1978-1979) Corporate Member, Institution of Chemical Engineers Member, judging panel. SET for Britain and Europe award (2005-present)

研究领域

The central theme of Eli’s research has been to investigate the interaction between cell engineering and fermentation and downstream processing. The early focus was on the impact of recombinant technology on the processing of macromolecules; in particular proteins with specific attention to the way fermentation strategy may control the location of the protein, and its titre in order to adapt the best harvesting strategy and enable the provision of generic process options for downstream separation. The range of organisms studied included both microbial and fungal systems. This extended into processing of recombinant macromolecules specifically antibodies to transgenic plants as an alternative route to production. The largest challenge is in the early stages where the material for processing is to be prepared and then separated. The work has complemented the mainstream microbial and animal cell studies. Her research has been the basis for further study on creating a framework for rapid choice of a whole bioprocess prior to detailed design. As a one of the 5 principal investigators of IMRC for bioprocessing (2007-2012), she brought a new theme based on the specific, knowledge-based design of cells and their propagation in bioreactors in response to the needs and demands of downstream/ purification stages. This is a novel approach to the whole process design. Hitherto, downstream operations have had to deal with every increasing higher concentrations of biomass and titres of products, and the removal of challenging contaminants arising from early stage processing, whilst operating within the constraints set by regulatory authorities. The new approach has aimed at easing this burden. In further research grants, the challenge of harnessing complex, large plasmids and phages which are potential candidates for a new generation of biopharmaceuticals such as multivalent vaccines and debilitating conditions such as muscular dystrophy has been considered. Their characteristics such as size are setting new barriers both at the synthesis and separation stages due to the likelihood of very low titres, a high level of genomic contamination and high potential degradation. Currently, her collaboration with leading centres in the UK and Europe (Warwick, Kent, Oulu, Zurich) is helping scientists from these centres to re-engineer host cell organisms so they can be used to enhance or create alternative routes for bioprocess sequences.

近期论文

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A cell engineering strategy to enhance supercoiled plasmid DNA production for gene therapy Journal article; 2016; Biotechnology and Bioengineering Links: IRIS | UCL Discovery | DOI Authors: Hassan S,Keshavarz-Moore E,Ward J click to collapse Application of Magnetic Field for Improvement of Microbial Productivity Journal article; 2016; 5TH INTERNATIONAL SYMPOSIUM ON INDUSTRIAL BIOTECHNOLOGY (IBIC 2016) Links: IRIS | UCL Discovery | Author's | DOI Authors: Mohtasham P,Keshavarz-Moore E,Kale I,Keshavarz T click to collapse Influence of Pichia pastoris cellular material on polymerase chain reaction performance as a synthetic biology standard for genome monitoring Journal article; 2016; JOURNAL OF MICROBIOLOGICAL METHODS Links: IRIS | UCL Discovery | Author's | DOI Authors: Templar A,Woodhouse S,Keshavarz-Moore E,Nesbeth DN click to collapse Optimising cell design steps for efficient removal of E. coli host cell DNA impurities from Fab' fragment process streams. Conference presentation; 2016; Links: IRIS | UCL Discovery | Publisher Authors: Schofield D,Sirka E,Ward JM,Keshavarz-moore E,Nesbeth DN click to collapse A non-chromatographic method for the removal of endotoxins from bacteriophages Journal article; 2015; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | Author's | DOI Authors: Branston SD,Wright J,Keshavarz-Moore E click to collapse A non-chromatographic method for the removal of endotoxins from bacteriophages Journal article; 2015; Biotechnology and Bioengineering Links: IRIS | DOI Authors: Branston SD,Wright J,Keshavarz-Moore E click to collapse Efficient export of human growth hormone, interferon ?2b and antibody fragments to the periplasm by the Escherichia coli Tat pathway in the absence of prior disulfide bond formation. Journal article; 2015; Biochim Biophys Acta Links: IRIS | UCL Discovery | Author's | DOI Authors: Alanen HI,Walker KL,Lourdes Velez Suberbie M,Matos CF,Bönisch S,Freedman RB,Keshavarz-Moore E,Ruddock LW,Robinson C click to collapse Breakage of transgenic tobacco roots for monoclonal antibody release in an ultra-scale down shearing device Journal article; 2014; Biotechnology and Bioengineering Links: IRIS | UCL Discovery | DOI Authors: Hassan S,Keshavarz-Moore E,Ma J,Thomas C click to collapse Breakage of transgenic tobacco roots for monoclonal antibody release in an ultra-scale down shearing device. Journal article; 2014; Biotechnol Bioeng Links: IRIS | UCL Discovery | Author's | DOI Authors: Hassan S,Keshavarz-Moore E,Ma J,Thomas C click to collapse Efficient export of prefolded, disulfide-bonded recombinant proteins to the periplasm by the Tat pathway in Escherichia coli CyDisCo strains. Journal article; 2014; Biotechnol Prog Links: IRIS | UCL Discovery | Author's | DOI Authors: Matos CF,Robinson C,Alanen HI,Prus P,Uchida Y,Ruddock LW,Freedman RB,Keshavarz-Moore E click to collapse Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration Journal article; 2013; Journal of Membrane Science Links: IRIS | UCL Discovery | DOI Authors: Affandy A,Keshavarz-Moore E,Versteeg HK click to collapse Determination of the survival of bacteriophage M13 from chemical and physical challenges to assist in its sustainable bioprocessing Journal article; 2013; Biotechnology and Bioprocess Engineering Links: IRIS | UCL Discovery | DOI Authors: Branston SD,Stanley EC,Ward JM,Keshavarz-Moore E click to collapse High-level secretion of a recombinant protein to the culture medium with a Bacillus subtilis twin-arginine translocation system in Escherichia coli Journal article; 2013; FEBS Journal Links: IRIS | UCL Discovery | DOI Authors: Albiniak AM,Matos CFRO,Branston SD,Freedman RB,Keshavarz-Moore E,Robinson C click to collapse High-level secretion of a recombinant protein to the culture medium with a Bacillus subtilis twin-arginine translocation system in Escherichia coli. Journal article; 2013; FEBS J Links: IRIS | UCL Discovery | Author's | DOI Authors: Albiniak AM,Matos CF,Branston SD,Freedman RB,Keshavarz-Moore E,Robinson C click to collapse Importin-7 Mediates Nuclear Trafficking of DNA in Mammalian Cells Journal article; 2013; Traffic Links: IRIS | UCL Discovery | DOI Authors: Dhanoya A,Wang T,Keshavarz-Moore E,Fassati A,Chain BM click to collapse Ultra scale-down device to predict dewatering levels of solids recovered in a continuous scroll decanter centrifuge Journal article; 2013; Biotechnology Progress Links: IRIS | UCL Discovery | DOI Authors: Lopes AG,Keshavarz-Moore E click to collapse Ultra scale-down device to predict dewatering levels of solids recovered in a continuous scroll decanter centrifuge. Journal article; 2013; Biotechnol Prog Links: IRIS | UCL Discovery | Author's | DOI Authors: Lopes AG,Keshavarz-Moore E click to collapse An ultra scale-down approach to assess the impact of the choice of recombinant P. pastoris strain on dewatering performance in centrifuges. Journal article; 2012; Biotechnol Prog Links: IRIS | UCL Discovery | Author's | DOI Authors: Lopes AG,Khan N,Liddell J,Keshavarz-Moore E click to collapse Growth and productivity impacts of periplasmic nuclease expression in an Escherichia coli Fab' fragment production strain. Journal article; 2012; Biotechnol Bioeng Links: IRIS | UCL Discovery | Author's | DOI Authors: Nesbeth DN,Perez-Pardo MA,Ali S,Ward J,Keshavarz-Moore E click to collapse High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli Journal article; 2012; Biotechnology and Bioengineering Links: IRIS | UCL Discovery | DOI Authors: Matos CFRO,Branston SD,Albiniak A,Dhanoya A,Freedman RB,Keshavarz-Moore E,Robinson C click to collapse High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli. Journal article; 2012; Biotechnol Bioeng Links: IRIS | UCL Discovery | Author's | DOI Authors: Matos CF,Branston SD,Albiniak A,Dhanoya A,Freedman RB,Keshavarz-Moore E,Robinson C click to collapse Investigation of the impact of Tat export pathway enhancement on E. coli culture, protein production and early stage recovery. Journal article; 2012; Biotechnol Bioeng Links: IRIS | UCL Discovery | Author's | DOI Authors: Branston SD,Matos CF,Freedman RB,Robinson C,Keshavarz-Moore E click to collapse Precipitation of filamentous bacteriophages for their selective recovery in primary purification. Journal article; 2012; Biotechnol Prog Links: IRIS | UCL Discovery | Author's | DOI Authors: Branston S,Stanley E,Keshavarz-Moore E,Ward J click to collapse Prediction and verification of centrifugal dewatering of P. pastoris fermentation cultures using an ultra scale-down approach Journal article; 2012; Biotechnology and Bioengineering Links: IRIS | UCL Discovery | DOI Authors: Lopes AG,Keshavarz-Moore E click to collapse Prediction and verification of centrifugal dewatering of P. pastoris fermentation cultures using an ultra scale-down approach. Journal article; 2012; Biotechnol Bioeng Links: IRIS | UCL Discovery | Author's | DOI Authors: Lopes AG,Keshavarz-Moore E click to collapse