个人简介
Fellow of Institution of Chemical Engineers (1990)
Fellow of Royal Academy of Engineering (FREng) (1997)
Fellow of UCL (2001)
Director, EPSRC Innovative Manufacturing Research Centre for Bioprocessing, UCL (2002-2007)
Donald Medal for contributions to Biochemical Engineering, IChemE (2003)
Member of DTI/DoH Bioscience Innovation and Growth Team (BIGT) panel reporting on bioprocessing and biomanufacturing “Bioscience 2015 – Improving National Health, Increasing National Wealth” (2003-2004)
Chairman, EPSRC Innovative Manufacturing Research Centre for Bioprocessing, UCL (2007-)
Member of BERR Bioscience Innovation and Growth Team (BIGT) Review and Refresh Senior Working Group – Chair Sir David Cooksey (2008-2009)
Review panel chair of AIBN BioNanotechnology and Cell Processing,Australia,2010
Editorial Board Member Biotechnology and Bioengineering (2009-)
Dunnill award for outstanding contribution to UK bioprocessing,bioProcessUK (2011)
研究领域
Mike Hoare’s research focuses on creating novel routes to speed the translation from life science discovery to outcome for next generation therapeutics and help ensure wider access. This particularly concerns advanced biological macromolecular and cellular materials and their processing using a complex sequence of stages from a bioreactor through recovery and purification to product formulation ready for delivery. Current challenges for such bioprocess studies include: whole antibodies or fragments or fusions and other therapeutic proteins; modified human cells (especially for cancer therapy), disabled viruses; whole human cells for regenerative therapy.
Much of this research is based on discovering ultra scale–down methods to study operations such as centrifugation, membranes, filters and chromatography at the multi–millilitre scale and more recently at the micro–litre scale to allow integration with robotic devices. This work is managed collaboratively with Professor N.J. Titchener–Hooker, Dr. Y. Zhou, Dr. D.G. Bracewell, Dr. Eli Keshavarz–Moore and Professor J.M. Ward (Structural and Molecular Biology) within the EPSRC–funded IMRC Bioprocessing programme. This programme currently involves fifteen leading national and international biopharmaceutical companies and fourteen UCL and UK academic groups of a wide range of disciplines complementary to biochemical engineering. Examples include collaboration with: Dr Mark Smales, University of Kent on mass spectrometry analysis of variants of antibody structures and the effects of bioprocessing; Professor David Williams and Dr Henk Versteeg, Loughborough University on plasmid DNA formulation and aerosolisation; Dr Kerry Chester, Royal Free Hospital, Oncology on bioprocessing of fusion proteins for targeted cancer therapies; Dr Colin Love and Professor Rob Coffin, Biovex and Molecular Pathology UCL on disabled viruses for vaccines. A recently awarded Technology Strategy Board Programme is enabling further collaboration between Onyvax, LGC, Nottingham Trent University (Bioinformatics Centre) and UCL on human cell bioprocessing for cell–based vaccine cancer therapy. Such activities benefit from the IMRC, BiCE and Human Cell Therapy bioprocessing initiatives in the Department and the MBI programme for knowledge transfer and dissemination.
Particular research achievements have included the successful creation of ultra scale–down mimics for high–speed, continuous–flow centrifuges where, from the tens of millilitre scale it has been possible to predict the performance of industrial scale centrifuges servicing 20000L mammalian cell culture vessels (in collaboration with Lonza Biologics) and of precipitation vessels for industrial preparation of human–blood plasma fractions (in collaboration with Bio Products Laboratories). This offers the opportunity to specify with greater levels of confidence and at earlier stages in process development the type and size (or throughput) of centrifuges to be used and also the quality of clarified supernatant and how this may impact on later downstream processing stages. This work is now being taken forward to address the more complex targets described above and also considerations of how the necessary response to emergency healthcare needs (e.g. rapid production of a vaccine) may best be met.
Funding for the above research has come from the EPSRC, especially for the EPSRC IMRC Bioprocessing and EPSRC IDTC EngD Bioprocess Leadership programmes, BBSRC Targetted Bioprocessing Studentships, the Technology Strategy Board Programme and a range of company collaborators including Lonza Biologics, UCB Celltech, Biovex, Pall Life Sciences, ReNeuron, ImmunoBiology, GSK, Stabilitech.
近期论文
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Evaluation of options for harvest of a recombinant E-Coli fermentation producing a domain antibody using ultra scale-down techniques and pilot-scale verification Journal article; 2016; BIOTECHNOLOGY PROGRESS Links: IRIS | UCL Discovery | Author's | DOI Authors: Voulgaris I,Chatel A,Hoare M,Finka G,Uden M click to collapse
Ultra scale-down approaches to enhance the creation of bioprocesses at scale: impacts of process shear stress and early recovery stages Journal article; 2016; CURRENT OPINION IN CHEMICAL ENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: Rayat ACME,Chatel A,Hoare M,Lye GJ click to collapse
An Ultra Scale-Down Analysis of the Recovery by Dead-End Centrifugation of Human Cells for Therapy Journal article; 2015; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: Delahaye M,Lawrence K,Ward SJ,Hoare M click to collapse
Enhancing the selective extracellular location of a recombinant E-coli domain antibody by management of fermentation conditions Journal article; 2015; APPLIED MICROBIOLOGY AND BIOTECHNOLOGY Links: IRIS | Author's | DOI Authors: Voulgaris I,Finka G,Uden M,Hoare M click to collapse
Developing strategies to improve the recovery of a periplasmicaly expressed recombinant protein by manipulation of fermentation conditions Journal article; 2014; NEW BIOTECHNOLOGY Links: IRIS | UCL Discovery | Author's | DOI Authors: Voulgaris I,Chatel A,Finka G,Uden M,Hoare M click to collapse
Relationship between preparation of cells for therapy and cell quality using artificial neural network analysis Journal article; 2014; ARTIFICIAL INTELLIGENCE IN MEDICINE Links: IRIS | UCL Discovery | Author's | DOI Authors: Dhondalay GK,Lawrence K,Ward S,Ball G,Hoare M click to collapse
Ultra Scale-Down Characterization of the Impact of Conditioning Methods for Harvested Cell Broths on Clarification by Continuous Centrifugation-Recovery of Domain Antibodies from rec E. coli Journal article; 2014; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: Chatel A,Kumpalume P,Hoare M click to collapse
An ultra scale-down approach to study the interaction of fermentation, homogenization, and centrifugation for antibody fragment recovery from rec E. coli Journal article; 2013; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: Li Q,Mannall GJ,Ali S,Hoare M click to collapse
An ultra scale-down characterization of low shear stress primary recovery stages to enhance selectivity of fusion protein recovery from its molecular variants Journal article; 2013; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: Lau EC,Kong S,McNulty S,Entwisle C,Mcilgorm A,Dalton KA,Hoare M click to collapse
Study of a novel tube forming method for preparing engineered blood vessel. Journal article; 2012; Chemical Engineering Science Links: IRIS | UCL Discovery Authors: Eames I,Chau G,Landeryou M,Town M,Levy MS,Lye GJ,Mason C click to collapse
Use of focused acoustics for cell disruption to provide ultra scale-down insights of microbial homogenization and its bioprocess impact - recovery of antibody fragments from rec E. coli Journal article; 2012; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | UCL Discovery | Author's | DOI | Publisher Authors: Li Q,Aucamp JP,Tang A,Chatel A,Hoare M click to collapse
Mimic of a large-scale diafiltration process by using ultra scale-down rotating disc filter Journal article; 2010; Biotechnology Progress Links: IRIS | UCL Discovery | DOI Authors: Ma G,Aucamp J,Gerontas, S ,Craig A,Patel R,Hoare M,Zhou Y click to collapse
Rapid Whole Monoclonal Antibody Analysis by Mass Spectrometry: An Ultra Scale-Down Study of the Effect of Harvesting by Centrifugation on the Post-Translational Modification Profile Journal article; 2010; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: Reid CQ,Tait A,Baldascini H,Mohindra A,Racher A,Bilsborough S,Smales CM,Hoare M click to collapse
Sub-Population Analysis of Human Cancer Vaccine Cells-Ultra Scale-Down Characterization of Response to Shear Journal article; 2010; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: McCoy R,Ward S,Hoare M click to collapse
The release of single cells from embryoid bodies in a capillary flow device Journal article; 2010; Chemical Engineering Science Links: IRIS | UCL Discovery Authors: Papantoniou I,Hoare M,Veraitch FS click to collapse
Ultra Scale-Down Stress Analysis of the Bioprocessing of Whole Human Cells as a Basis for Cancer Vaccines Journal article; 2010; BIOTECHNOLOGY AND BIOENGINEERING Links: IRIS | UCL Discovery | Author's | DOI Authors: Acosta-Martinez JP,Papantoniou I,Lawrence K,Ward S,Hoare M click to collapse
A microwell-based method for predicting the effects of agitation and addition rates on the performance of large scale precipitation reactions Journal article; 2009; Chemical Engineering Science Links: IRIS | UCL Discovery Authors: Aucamp J,Papantoniou I,Town M,Hoare M click to collapse
Aerosol Delivery of Plasmid DNA: A Design-of-Experiments Study Conference ; 2009; MOLECULAR THERAPY Links: IRIS | UCL Discovery | Author's Authors: Arulmuthu ER,Versteeg HK,Williams DJ,Tait AS,Ward J,Hoare M click to collapse
Application of ultra scale-down technologies to investigate the effect harvest time and primary recovery by centrifugation have on the post-translational modification profile of a therapeutic monoclonal antibody produced in mammalian cells. Journal article; 2009; Links: IRIS | UCL Discovery Authors: Qvist C,Tait AS,Mohindra A,Racher A,Bilsborough S,Smales CM,Hoare M click to collapse
Prediction of large scale flux and transmission of crossflow filtration with ultra scale-down method Conference ; Links: IRIS | UCL Discovery Authors: Ma G,Hoare M,Zhou Y click to collapse
Regenerative medicine bioprocessing: concentration and behaviour of adherent cell suspensions and pastes. Journal article; 2009; Biotechnology and Bioengineering Links: IRIS | UCL Discovery Authors: Zoro BJH,Owen S,Drake RAL,Mason C,Hoare M click to collapse
Ultra scale-down approach to correct for dispersive and retentive effects in small-scale columns when predicting larger-scale elution profiles. Journal article; 2009; Biotechnology Progress Links: IRIS | UCL Discovery | Author's Authors: Hutchinson N,Chhatre S,Baldascini H,Davies J,Bracewell DG,Hoare M click to collapse
Ultra scale-down prediction using microwell technology of the industrial scale clarification characteristics by centrifugation of mammalian cell broths. Journal article; 2009; Biotechnology and Bioengineering Links: IRIS | UCL Discovery Authors: Tait AS,Aucamp JP,Bugeon A,Hoare M click to collapse
Ultra Scale-Down Studies of the Effect of Shear on Cell Quality; Processing of a Human Cell Line for Cancer Vaccine Therapy Journal article; 2009; BIOTECHNOLOGY PROGRESS Links: IRIS | UCL Discovery | Author's | DOI Authors: McCoy R,Hoare M,Ward S click to collapse
Ultra scale-down studies of the effect of shear on cell quality; processing of a human cell line for cancer vaccine therapy. Journal article; 2009; Biotechnology Progress Links: IRIS | UCL Discovery | DOI Authors: McCoy R,Ward S,Hoare M click to collapse
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