个人简介

Ph.D., University of Edinburgh, 2004

研究领域

Physical

Mass spectrometry, structures of (isolated) biomolecules Research in our group focuses on increasing the structural information from mass spectrometry measurements for bioanalytical applications. We make use of physical chemistry tools, such as lasers, and develop methods and instrumentation that allow other physical parameters of the ions to be characterized (e.g. infrared absorption, collision-cross-section), so that "more than the mass" of the ions can be determined. Topical projects include 1) the chemistry underlying peptide sequencing in mass spectrometers -- what chemical reactions take place in the gas phase, and how can they result in a loss of the sequence information? 2) the differentiation of carbohydrates, as these are among the most complex classes of (bio)molecules, thus remaining a challenging problem in mass spectrometry: we employ a multi-dimensional approach, where the structure of the sugars can be mapped based on the complementary structural information from multiple techniques.

近期论文

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'IRMPD action spectroscopy of alkali metal cation-cytosine complexes: effects of alkali metal cation size on gas phase conformation', B Yang, RR Wu, NC Polfer, G Berden, J Oomens, MT Rodgers; J. Am. Soc. Mass Spectrom., 2013, DOI: 10.1007/s13361-013-0689-7. 'Gas-phase structures of phosphopeptide ions: a difficult case', F Turecek, CL Moss, I Pikalov, R Pepin, K Gulyuz, NC Polfer, MF Bush, J Brown, J Williams, K Richardson; Int. J. Mass Spectrom., 2013, in press. 'Structure and reactivity of the glutathione radical cation: Radical rearrangement from the cysteine sulfur to the glutamic acid alpha-carbon atom', S Osburn, G Berden, J Oomens, K Gulyuz, NC Polfer, RAJ O'Hair, C Ryzhov; Chem. Plus Chem., 2013, DOI: 10.1002/cplu.201300057. 'Structures of biomolecular ions in the gas phase probed by infrared light sources', CN Stedwell, JF Galindo, AE Roitberg, NC Polfer; Ann. Rev. Anal. Chem., 2013, 6, 267-285. 'Metal cation binding to gas-phase pentaalanine: divalent ions restructure the complex', RC Dunbar, JD Steill, NC Polfer, J Oomens; J. Phys. Chem. A, 2013, 117, 1094-1101. 'Crown-complexation of protonated amino acids: Influence on IRMPD spectra', CN Stedwell, JF Galindo, K Gulyuz, AE Roitberg, NC Polfer; J. Phys. Chem. A, 2013, 117, 1181-1188. 'Metal ion binding to peptides: Oxygen or nitrogen sites?', RC Dunbar, NC Polfer, G Berden, J Oomens; Int. J. Mass Spectrom., 2012, 330, 71-77. 'Effect of phenol and acidic side chains on the protonation sites of b2 ions confirmed by IRMPD spectroscopy', D Wang, K Gulyuz, CN Stedwell, L Yu, NC Polfer; Int. J. Mass Spectrom., 2012, 330, 144-151. 'On the path to glycan conformer identification: Gas-phase study of the anomers of methyl glycosides of N-acetyl-D-glucosamine and N-acetyl-D-galactosamine', CS Contreras, NC Polfer, J Oomens, JD Steill, B Bendiak, JR Eyler; Int. J. Mass Spectrom., 2012, 330, 285-294. 'Screening for phosphorylated and non-phosphorylated peptides by infrared photodissociation spectroscopy', CN Stedwell, AL Patrick, K Gulyuz, NC Polfer; Anal. Chem., 2012, 84, 9907-9912. 'Defying entropy - forming large head-to-tail macrocycles in the gas phase', M Tirado, NC Polfer; Angew. Chem. Int. Ed., 2012, 51, 6436-6438. 'Peptide bond tautomerization induced by divalent metal ions: characterization of the iminol configuration', RC Dunbar, JD Steill, NC Polfer, G Berden, J Oomens; Angew. Chem. Int. Ed., 2012, 51, 4591-4593 'Competition between diketopiperazine and oxazolone formation in water loss products from ArgGly and GlyArg', S Zou, J Oomens, NC Polfer; Int. J. Mass Spectrom., 2012, 316-318, 12-17. 'Disfavoring Macrocycle b Fragments by Constraining Torsional Freedom: The "Twisted" Case of QWFGLM b6', M Tirado, J Rutters, X Chen, A Yeung, J van Maarseveen, JR Eyler, G Berden, J Oomens, NC Polfer; J. Am. Soc. Mass Spectrom., 2012, 23, 475-482. 'Photon-induced formation of molecular hydrogen from a neutral polycyclic aromatic hydrocarbon: 9,10-dihydroanthracene', Y Fu, J Szczepanski, NC Polfer; Astrophys. J., 2012, 744, 61-68. 'On the periodicity of monoisotopic mass isomers and isobars in proteomics', L Yu, Y-M Xiong, NC Polfer; Anal. Chem., 2011, 83, 8019-8023. 'Cyclic peptide as reference system for b ion structural analysis in the gas phase', X Chen, M Tirado, JD Steill, J Oomens, NC Polfer; J. Mass Spectrom., 2011, 46, 1011-1015. 'Hybrid quadrupole mass filter/quadrupole ion trap/time-of-flight-mass spectrometer for infrared multiple photon dissociation spectroscopy of mass-selected ions', K Gulyuz, CN Stedwell, D Wang, NC Polfer; Rev. Scie. Instrum., 2011, 82, 054101. 'Diagnostic NH and OH vibrations for oxazolone and diketopiperazine structures: b2 from protonated triglycine', D Wang, K Gulyuz, CN Stedwell, NC Polfer; J. Am. Soc. Mass Spectrom., 2011, 22, 1197-1203 'On the relevance of peptide sequence permutations in shotgun proteomics studies', L Yu, Y Tan, Y Tsai, DR Goodlett, NC Polfer; J. Proteome Res., 2011, 10, 2409-2416.