个人简介

B.S., Bucknell University, 1986 M.S., Bucknell University, 1987 Ph.D., Cornell University, 1991

研究领域

Biochemistry

Enzymatic asymmetric synthesis, Smart nanostructures, Starch biosynthesis We develop new methods for asymmetric organic synthesis based on enzymes. These efforts include the discovery of novel enzymes by computational and experimental approaches, the development of strategies to rapidly assess their catalytic properties, their applications to organic synthesis and solving problems associated with reaction scale-up. A second part of our research focuses on developing "smart" nanostructures that assemble and disassemble in response to environmental cues. This project is a collaboration with groups in the Department of Chemistry (Martin) and the Department of Anesthesiology (Dennis and Rogers). We combine our expertise in synthesis and biochemistry with our collaborators' strengths in nanotechnology and medicine.

近期论文

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Structural and Catalytic Characterization of Pichia stipitis OYE 2.6, a Useful Catalyst for Asymmetric Alkene Reductions. Y.A. Pompeu, B. Sullivan, A.Z. Walton and J.D. Stewart, Adv. Synth. Catal. 2012, in press. Biocatalytic Reductions of Baylis-Hillman Adducts. A.Z. Walton, W.C. Conerly, Y.A. Pompeu, B. Sullivan and J.D. Stewart ACS Catalysis 2011, 1, 989-993 (coverarticle). Towards Preparative-Scale, Biocatalytic Alkene Reductions. D.J. Bougioukou, A.Z. Walton and J.D. Stewart, Chem. Commun. 2010, 46, 8558-8560. Site Saturation Mutagenesis of Tryptophan 116 of Saccharomyces pastorianus Old Yellow Enzyme Uncovers Stereocomplementary Variants. S.K. Padhi, D.J. Bougioukou and J.D. Stewart, J. Am. Chem. Soc. 2009, 131, 3271-3280. Opposite Stereochemical Courses for Enzyme-Mediated Alkene Reductions of an Enantiomeric Substrate Pair. D.J. Bougioukou and J.D. Stewart, J. Am. Chem. Soc., 2008, 130, 7655-7658. Reductions of Cyclic beta-Keto Esters by Individual Saccharomyces cerevisiae Dehydrogenases and a Chemo-Enzymatic Route to (1R,2S)-2-Methyl-1-cyclohexanol. S.K. Padhi, I.A. Kaluzna, D. Buisson, R. Azerad and J.D. Stewart, Tetrahedron: Asymmetry 2007, 18, 2133-2138. Asymmetric Bioreductions of Beta-Nitroacrylates as a Route to Beta2-Amino Acids. M.A. Swiderska and J.D. Stewart, Org. Lett. 2006, 8, 6131-6133. Stereoselective Enone Reductions by Saccharomyces carlsbergensis Old Yellow Enzyme. M.A. Swiderska and J.D. Stewart, J. Mol. Catal. B: Enzymatic 2006, 42, 52-54. Application of Newly-Available Bio-Reducing Agents to the Synthesis of Chiram Hydroxy-beta-Lactams; Model for Aldose Reductase Stereochemistry. M.M. Kayser, M. Drolet and J.D. Stewart, Tetrahedron: Asymmetry 2005, 16, 4004-4009.. Enantiodivergent, Biocatalytic Routes to Both Taxol Side Chain Antipodes. B.D. Feske, I.A. Kaluzna and J.D. Stewart, J. Org. Chem. 2005, 70, 9654-9657. Chemoenzymatic Formal Total Synthesis of (-)-Bestatin. B.D. Feske and J.D. Stewart, Tetrahedron: Asymmetry 2005, 16, 3124-3127. Assessing Substrate Acceptance and Enantioselectivity of Yeast Reductases in Reactions with Substituted alpha-Keto-beta-Lactams. Y.Yang, M.M. Kayser, F.D. Rochon, S. Rodriguez and J.D. Stewart, J. Mol. Catal. B: Enzymatic 2005, 32, 167-174. Stereoselective, Biocatalytic Reductions of alpha-Chloro-beta-Keto Esters. I.A. Kaluzna, W. Wittayanan, B.D. Feske, I. Ghiviriga and J.D. Stewart, J. Org. Chem. 2005, 70, 342-345. A Systematic Investigation of Saccharomyces cerevisiae Enzymes Catalyzing Carbonyl Reductions. I.A. Kaluzna, T. Matsuda, A.K. Sewall and J.D. Stewart, J. Am. Chem. Soc. 2004, 126, 12827-12832. Regio- and Enantioselective Reduction of t-Butyl 6-Chloro-3,5-dioxohexanoate With Baker’s Yeast. M. Wolberg, I.A. Kaluzna, M. Mueller and J.D. Stewart, Tetrahedron: Asymmetry 2004, 15, 2825-2828. Enantioselective Reductions of Ethyl 2-Oxo-4-phenylbutyrate by Saccharomyces cerevisiae Dehydrogenases. I. Kaluzna, A.A. Andrew, M. Bonilla, M.R. Martzen and J.D. Stewart, J. Mol. Catal. B: Enzymatic, 2002, 17, 101-105. Purification and Identification of an Escherichia coli beta-Keto Ester Reductase as 2,5-Diketo-D-gluconate Reductase YqhE. M. Habrych, S. Rodriguez and J.D. Stewart, Biotechnol. Progress 2002, 18, 257-261. Highly Stereoselective Reagents for beta-Keto Ester Reductions by Genetic Engineering of Baker’s Yeast. S. Rodriguez, M.M. Kayser and J.D. Stewart, J. Am. Chem. Soc., 2001, 123, 1547-1555. Asymmetric Synthesis of beta-Hydroxy Esters and alpha-Alkyl-beta-hydroxy Esters by Recombinant Escherichia coli Expressing Enzymes from Baker’s Yeast. S. Rodriguez, K.T. Schroeder, M.M. Kayser and J.D. Stewart, J. Org. Chem., 2000, 65, 2586-2587.