de Graaf, Barend HJ - Cardiff University

研究领域

Fertilization in higher plants is the result of a series of successful interactions between pollen and pistils of the same species. An understanding of pollen tube growth and the interactions of pollen and growing pollen tubes with the pistil is fundamental to basic plant sciences. Sexual reproduction in flowering plants involves species specific communication events. Upon pollen landing, pollen and pistil are being recognized as 'own' or 'foreign' which results in acceptance or rejection, respectively. After a successful pollination and compatible interaction between growing pollen tubes and several pistil tissues, fertilization occurs and portions of the pistil develop into a fruit containing the seeds. To date very little is known about which pollen and pistil proteins play a role in the communication between both partners during compatible pollen pistil interactions. Moreover, nothing is known about how male and female partners of different plant species discriminate between own and foreign. We investigate the mechanisms of pollen and pistil acceptance and rejection during plant reproduction by identifying pistil and pollen proteins involved in these processes. Furthermore we try to characterize pollen and pistil proteins that mediate successful pollen-pistil interactions and in a species specific manner.

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Nieuwland, J.et al. 2012. Phytotracker, an information management system for easy recording and tracking of plants, seeds and plasmids. Plant Methods 8, article number: 43. (10.1186/1746-4811-8-43) pdf De Graaf, B.et al. 2012. The Papaver self-incompatibility pollen S-determinant, PrpS, functions in 'Arabidopsis thaliana'. Current Biology 22(2), pp. 154-159. (10.1016/j.cub.2011.12.006) pdf Nieuwland, J.et al. 2011. Plant reproduction: does size matter?. New Phytologist 190(4), pp. 812-815. (10.1111/j.1469-8137.2011.03749.x) Wheeler, M.et al. 2009. Identification of the pollen self-incompatibility determinant in Papaver rhoeas. Nature 459(7249), pp. 992 -995. (10.1038/nature08027) Cheung, A.et al. 2008. The dynamic pollen tube cytoskeleton: Live cell studies using actin-binding and microtubule-binding reporter proteins. Molecular Plant 1(4), pp. 686-702. (10.1093/mp/ssn026) Bosch, M.et al. 2008. Signalling to programmed cell death in self-incompatible pollen. Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology 150(3, S1), pp. S144-S144. (10.1016/j.cbpa.2008.04.361) De Graaf, B.et al. 2006. Self-incompatibility in Papaver targets soluble inorganic pyrophosphatases in pollen. Nature 444, pp. 490-493. (10.1038/nature05311) De Graaf, B.et al. 2005. Rab11 GTPase-regulated membrane trafficking is crucial for tip-focused pollen tube growth in tobacco. The Plant Cell 17(9), pp. 2564-2579. (10.1105/tpc.105.033183) De Graaf, B.et al. 2004. The PELPIII glycoproteins in Solanaceae: stylar expression and transfer into pollen tube walls. Sexual Plant Reproduction 16(5), pp. 245-252. (10.1007/s00497-003-0196-2) De Graaf, B.et al. 2003. Characterization and localization of the transmitting tissue-specific PELPIII proteins of nicotiana tabacum. Journal of Experimental Botany 54(380), pp. 55-63. (10.1093/jxb/erg002) Cheung, A.et al. 2002. Rab2 GTPase regulates vesicle trafficking between the endoplasmic reticulum and the golgi bodies and is important to pollen tube growth. The Plant Cell 14(4), pp. 945-962. (10.1105/tpc.000836) Wu, H.et al. 2001. Hydroxyproline-rich glycoproteins in plant reproductive tissues: structure, functions and regulation. Cellular and Molecular Life Sciences 58(10), pp. 1418-1429. (10.1007/PL00000785) De Graaf, B. H. J., Derksen, J. W. M. and Mariani, C. 2001. Pollen and pistil in the progamic phase. Sexual Plant Reproduction 14(1-2), pp. 41-55. (10.1007/s004970100091)