个人简介

Education: B.S., 1969, Tufts University; Ph.D., 1976, University of Chicago Awards: A.P. Sloan Fellow, 1980 Dreyfus Teacher-Scholar Fellow, 1981 Dean's Award for Distinguished Teaching, 1982 Presidential Young Investigator Award, 1984 American Society of Photobiology Award, 1992 Five College Lecturer in Chemistry, 1993 NIH Merit Award, 1994; ACS Cope Scholar Award, 1995 American Academy of Arts and Sciences, 1997 Fellow of the Biophysical Society, 2007 K. Plyler Prize, 2008 Member, National Academy of Sciences, 2008 Elected Fellow of the Royal Society of Chemistry, 2009 American Chemical Society E. Bright Wilson Award in Spectroscopy, 2013

研究领域

Bio-nanotechnology/Biophysical/Physical

My laboratory investigates the structure and function of biological systems using many tools and methods, always with a strong physical perspective. Four interconnected themes are being pursued. First, we are broadly interested in electrostatics in proteins and how electrostatics affect function. Our current work uses vibrational probes whose sensitivity to electric fields can be calibrated by Stark spectroscopy. Probes are introduced on inhibitors, by modification of amino acids and by incorporation of unnatural amino acids, and these are used to map electric fields for comparison with simulations and as probes of the role of protein electrostatics in enzymatic catalysis. Second, we use supported lipid bilayers as mimics for cell surfaces and as tools in biotechnology. A broad vision is to engineer interfaces between hard surfaces and soft materials, ultimately leading to sophisticated biocompatible interfaces that can be used to control, interrogate or organize complex living systems. Recent work addresses the formation of domains and protein associations with these domains, interactions of DNA, proteins and cells with supported bilayers, and the mechanism of vesicle fusion. This work has motivated the development of advanced optical microscopy methods for probing the interface between membranes on solid supports and cell membranes. A novel type of imaging mass spectrometry is being applied to characterize the lateral organization and composition of bilayers and associated membranes with 50 nm resolution. Third, we have a long-standing interest in the mechanism of light-driven, long-distance electron transfer in photosynthetic reaction centers, one of the fastest known reactions. This is being studied by femtosecond fluorescence and transient absorption spectroscopy, site-specific mutagenesis and vibrational Stark spectroscopy. Fourth, related methods are also being used to probe excited state dynamics, solvation, and electronic structure in variants of green fluorescent protein (GFP), widely used in cell biology. Recent work focuses on discoveries of photo-association and -dissociation of split GFPs that can be used to modulate complex functions using light, novel GFPs with alternative folds, and the mechanism(s) of ground and excited state proton transfer. Please visit our web site for more information.

近期论文

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"Structural and Spectroscopic Analysis of the Kinase Inhibitor Bosutinib and an Isomer of Bosutinib Binding to the Abl Tyrosine Kinase Domain", Nicholas M. Levinson, and Steven G. Boxer, PLoS ONE 7, e29828 (2012).[C&ENews story] "A Solvatochromic Model Calibrates Nitriles' Vibrational Frequencies to Electrostatic Fields", Sayan Bagchi, Stephen D. Fried, and Steven G. Boxer, J. Am. Chem. Soc., 134, 10373-10376 (2012). "Photochemistry of a Bacterial Photosynthetic Reaction Center Missing the Initial Bacteriochlorophyll Electron Acceptor", Brett Carter, Steven G. Boxer, Dewey Holten, and Christine Kirmaier, J. Phys. Chem. B 116, 9971-9982 (2012). "Site-Specific Measurement of Water Dynamics in the Substrate Pocket of Ketosteroid Isomerase Using Time-Resolved Vibrational Spectroscopy", Santosh Kumar Jha, Minbiao Ji, Kelly J. Gaffney, and Steven G. Boxer, J. Phys. Chem. B, 116, 11414-11421 (2012). "Experimental quantification of electrostatics in X-H⋅⋅⋅π hydrogen bonds", Miguel Saggu, Nicholas M. Levinson, and Steven G. Boxer, J. Am. Chem. Soc., 134, 18986-18997 (2012). "Formation and Analysis of Topographical Domains Between Lipid Membranes Tethered by DNA Hybrids of Different Lengths", Minsub Chung, Bon Jun Koo, and Steven G. Boxer, Faraday Discussion, 161 , 333-345 (2013). "Colocalization of the Ganglioside GM1 and Cholesterol Detected by Secondary Ion Mass Spectrometry", Mónica M. Lozano, Zhao Liu, Andreas Janshoff, Krishna Kumar, and Steven G. Boxer, J. Am. Chem. Soc., 135 , 5620-5630 (2013).[C&E News story] “Individual Vesicle Fusion Events Mediated by Lipid-anchored DNA”, Bettina van Lengerich, Robert J. Rawle, Poul Martin Bendix, Minsub Chung, and Steven G. Boxer, Biophysical Journal , 105 , 409–419 (2013). “Quantitative Dissection of Hydrogen Bond-Mediated Proton Transfer in the Ketosteroid Isomerase Active Site”, Paul A. Sigala, Aaron T. Fafarman, Jason P. Schwans, Stephen D. Fried, Timothy D. Fenn, Jose M. M. Caaveiro, Brandon Pybus, Dagmar Ringe, Gregory A. Petsko, Steven G. Boxer, and Daniel Herschlag, Proceedings of the National Academy of Sciences, 110, E2552-E2561 (2013). “A Thermodynamic Framework For Identifying Free Energy Inventories of Enzyme Catalytic Cycles”, Stephen D. Fried, Steven G. Boxer, Proceedings of the National Academy of Sciences, 110, 12271-12276 (2013). “Measuring Electrostatic Fields in Both Hydrogen Bonding and non-Hydrogen Bonding Environments using Carbonyl Vibrational Probes,” Stephen D. Fried, Sayan Bagchi, Steven G. Boxer, Journal of the American Chemical Society, 135, 11181-11192 (2013). “GFP Variants with Alternative β‑Strands and Their Application as Light-driven Protease Sensors: A Tale of Two Tails”, Keunbong Do and Steven G. Boxer, Journal of the American Chemical Society, 135, 10226-10229 (2013). “DNA-Based Patterning of Tethered Membrane Patches”, Laura D. Hughes and Steven G. Boxer, Langmuir, 29, 12220-12227 (2013). “Calculations of the Electric Fields in Liquid Solutions”, Stephen D. Fried, Lee-Ping Wang, Steven G. Boxer, Pengyu Ren, and Vijay S. Pande, J. Phys. Chem. B, 117, 16236-16248 (2013). “A conserved water-mediated hydrogen bond network defines bosutinib’s kinase selectivity”, Nicholas M Levinson & Steven G. Boxer, Nat. Chem. Biol., 10, 127-132 (2014). “Choose Your Label wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers”, Laura D. Hughes, Robert J. Rawle, and Steven G. Boxer , PLoS ONE, 9, e87649 (2014). “Putative Hydrogen Bond to Tyrosine M208 in Photosynthetic Reaction Centers from Rhodobacter capsulatus Significantly Slows Primary Charge Separation”, Miguel Saggu, Brett Carter, Xiaoxue Zhou, Kaitlyn Faries, Lynette Cegelski, Dewey Holten, Steven G. Boxer, and Christine Kirmaier, J. Phys. Chem. B, 118, 6721-6732 (2014). “Ground-state Proton Transfer Kinetics in Green Fluorescent Protein (GFP)”, Luke M. Oltrogge, Quan Wang, and Steven G. Boxer, Biochemistry, 53, 5947-5957 (2014). “Extreme Electric Fields Power Catalysis in the Active Site of Ketosteroid Isomerase”, Stephen D. Fried, Sayan Bagchi, Steven G. Boxer, Science, 346, 1510-1514 (2014).[Perspective] “Quantum delocalization of protons in the hydrogen-bond network of an enzyme active site”, Lu Wang, Stephen D. Fried, Steven G. Boxer, and Thomas E. Markland, Proceedings of the National Academy of Sciences, 111, 18454-18459 (2014).