Amici-Dargan, Sheila - Cardiff University

个人简介

I graduated with a BSc hons in Cell Biology from the University of East Anglia (UEA) in 1998 and completed a PhD in Biophysics at UEA in 2001, in the laboratories of Prof Alan Dawson FRS and Dr Edward Lea. My PhD involved setting up a novel technique (excised-patch single-channel recordings of purified receptors reconstituted into giant liposomes) to investigate the pharmacological regulation of IP3 receptors. In 2002 I moved to the USA to work as a postdoctoral researcher in the laboratory of Professor Ian Parker FRS at the University of California Irvine (UCI), where my main research projects involved using line-scan confocal imaging and flash photolysis of caged compounds in Xenopus oocytes to investigate modulation of IP3-mediated calcium signals by calcium buffers and neuronal Ca2+ binding proteins. On returning to the UK, I took up a temporary post at the Biochemical Society as a Professional and Education Projects Manager, which involved running scientific events, maintaining educational websites and forging links with external organisations. In 2004 I joined the University of Bristol, where I conducted postdoctoral research in the laboratories of laboratories of Professor Graham Collingridge FRS and Professor David Jane. My research there focused on the role of glutamate receptors in synaptic plasticity in hippocampal brain slices using simultaneous 2-photon microscopy, whole-cell electrophysiology and calcium imaging. In 2009 I was appointed to a University Lectureship in Physiology at Cardiff University. I completed a postgraduate certificate in higher education in 2012 (awarded Graduate School of Education, Bristol University), and was promoted to Senior Lecturer at Cardiff University in 2013. I currently teach Physiology and Neuroscience to undergraduate students, am the degree scheme coordinator for Physiology, academic lead for enrolment and module leader for BI1002 Structure and function of living organisms. I have active interests in science outreach and pedagogic research and am a member of the Physiological Society’s Education and Outreach committee.

研究领域

My scientific lab-based research is described on my overview page. Most recently I was using simultaneous electrophysiology and 2-photon calcium imaging in hippocampal brain slices to investigate the role of ionotropic glutamate receptors in long-term potentiation (LTP) and long-term depression (LTD).

近期论文

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Yhnell, E.et al. 2016. The impact of attaining the Welsh Baccalaureate Advanced Diploma on academic performance in bioscience higher education. International Journal of Science Education 38(1), pp. 156-169. (10.1080/09500693.2015.1135353) pdf Sherwood, J.et al. 2012. Differences in kainate receptor involvement in hippocampal mossy fibre long-term potentiation depending on slice orientation. Neurochemistry International 61(4), pp. 482-489. (10.1016/j.neuint.2012.04.021) Nisticò, R.et al. 2011. Synergistic interactions between kainate and mGlu receptors regulate bouton Ca2+ signalling and mossy fibre LTP. Scientific Reports 1, article number: 103. (10.1038/srep00103) Bruno, L.et al. 2010. Quantifying calcium fluxes underlying calcium puffs in Xenopus laevis oocytes. Cell Calcium 47(3), pp. 273-286. (10.1016/j.ceca.2009.12.012) Dargan, S.et al. 2009. [Online comment on] Role of glutamate autoreceptors at hippocampal mossy fiber synapses. Neuron 2009(27 Mar) Dargan, S.et al. 2009. ACET is a highly potent and specific kainate receptor antagonist: Characterisation and effects on hippocampal mossy fibre function. Neuropharmacology 56(1), pp. 121-130. (10.1016/j.neuropharm.2008.08.016) Amici, M.et al. 2009. Neuronal calcium sensors and synaptic plasticity. Biochemical Society Transactions 37(6), pp. 1359-1363. (10.1042/BST0371359) Dargan, S. and Amici, M. 2009. Role of kainate autoreceptors in short-term plasticity at hippocampal mossy fiber synapses. Journal of Neuroscience 29(18), pp. 5713-5715. (10.1523/JNEUROSCI.0549-09.2009) Nistico, R.et al. 2009. GluK1 receptor antagonists and hippocampal mossy fibre function. International Review of Neurobiology 85, pp. 13-27. (10.1016/S0074-7742(09)85002-2) Bartlett, T.et al. 2007. Differential roles of NR2A and NR2B-containing NMDA receptors in LTP and LTD in the CA1 region of two-week old rat hippocampus. Neuropharmacology 52(1), pp. 60-70. (10.1016/j.neuropharm.2006.07.013) Rose, H.et al. 2006. 'Trigger’ events precede calcium puffs in Xenopus Oocytes. Biophysical Journal 91(11), pp. 4024-4032. (10.1529/biophysj.106.088872) Fraiman, D.et al. 2006. Analysis of puff dynamics in oocytes: interdependence of puff amplitude and interpuff interval. Biophysical Journal 90(11), pp. 3897-3907. (10.1529/biophysj.105.075911) Dargan, S. L., Schwaller, B. and Parker, I. 2004. Spatiotemporal patterning of IP3-mediated Ca2+ signals in Xenopus oocytes by Ca2+-binding proteins. The Journal of Physiology 556(2), pp. 447-461. (10.1113/jphysiol.2003.059204) Dargan, S. L. and Parker, I. 2003. Buffer kinetics shape the spatiotemporal pattern of IP3-evoked Ca2+ signals. The Journal of Physiology 553(3), pp. 775-788. (10.1113/jphysiol.2003.054247) Dargan, S., Lea, E. J. A. and Dawson, A. P. 2002. Modulation of type-1 Ins(1,4,5)P3 receptor channels by the FK506-binding protein, FKBP12. Biochemical Journal 361(2), pp. 401-407. (10.1042/0264-6021:3610401) Thrower, E.et al. 2000. Interaction of luminal calcium and cytosolic ATP in the control of type 1 inositol (1,4,5)-trisphosphate receptor channels. Journal of Biological Chemistry 275(46), pp. 36049-36055. (10.1074/jbc.M000970200)