研究领域
Human IPSC models of neurodegenerative disease
Neurodegenerative diseases are devastating for patients, families and society. Understanding the mechanistic basis of these diseases is fundamental to future drug development and therapeutic intervention. My group works on two diseases – Huntington’s (HD) and Alzheimer’s (AD). HD is an inherited genetic disorder caused by mutation in a single gene called huntingtin (Htt), in contrast AD is a multifactorial disease with a significant degree of risk gene heritability. A major research challenge is to model these diseases, to gain an understanding of disease mechanisms and to build platforms for future drug discovery and testing. To address this challenge my research group has focussed on the development of human cell models of disease. We take advantage of landmark ‘induced pluripotent stem cell technologies’ that ultimately enable disease models to be derived from patients – thereby directly integrating patient genetics into the models.
My group has longstanding expertise in developmental neuroscience and stem cell biology. We harness this expertise to control stem cell differentiation to generate highly defined disease models that comprise the most disease–relevant cells for study. We collaborate extensively working within consortia for HD disease modeling (NINDS/CHDI HD iPS Consortium) and cell therapy (FP7 Repair HD) and for AD in the MRC/Dementia Platform UK (DPUK) stem cell network. For each program we provided expertise in stem cell differentiation, CRISPR-Cas9 genome editing and neurobiology. Our work on HD has led to novel biomarker discovery, provided progressive phenotyping, identified physiological deficits in the human neuronal models and led to engagement with industry to translate platforms for drug discovery.
近期论文
查看导师新发文章
(温馨提示:请注意重名现象,建议点开原文通过作者单位确认)
Bowles, K.et al. 2017. SMAD transcription factors are altered in cell models of HD and regulate HTT expression. Cellular Signalling 31, pp. 1-14. (10.1016/j.cellsig.2016.12.005) pdf
Kemp, P.et al. 2016. Improving and accelerating the differentiation and functional maturation of human stem cell-derived neurons: role of extracellular calcium and GABA. The Journal of Physiology 594(22), pp. 6583-6594. (10.1113/JP270655) pdf
Howard-Jones, R.et al. 2016. Integration-free reprogramming of lamina propria progenitor cells. Journal of Dental Research 95(8), pp. 882-888. (10.1177/0022034516637579) pdf
Gee, H.et al. 2016. FAT1 mutations cause a glomerulotubular nephropathy. Nature Communications 7, article number: 10822. (10.1038/ncomms10822)
Telezhkin, V.et al. 2015. Forced cell-cycle exit and modulation of GABAA, CREB and GSK3? signaling promote functional maturation of induced pluripotent stem cell-derived neurons. American Journal of Physiology - Cell Physiology , article number: ajpcell.00166.2015. (10.1152/ajpcell.00166.2015)
Straccia, M.et al. 2015. Quantitative high-throughput gene expression profiling of human striatal development to screen stem cell-derived medium spiny neurons. Molecular Therapy - Methods & Clinical Development 2, article number: 15030. (10.1038/mtm.2015.30)
Celiz, A.et al. 2015. Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation. Advanced Materials 27(27), pp. 4006-4012. (10.1002/adma.201501351)
Jones, A.et al. 2015. Evidence for bystander signalling between human trophoblast cells and human embryonic stem cells. Scientific Reports 5, article number: 11694. (10.1038/srep11694) pdf
Mort, M.et al. 2015. Huntingtin exists as multiple splice forms in human brain. Journal of Huntington's Disease 4(2), pp. 161-171. (10.3233/JHD-150151) pdf
Rushton, D.et al. 2013. Stimulation of GABA-induced Ca2+ influx enhances maturation of human induced pluripotent stem cell-derived neurons. PLoS ONE 8(11), pp. e81031. (10.1371/journal.pone.0081031) pdf
Mattis, V.et al. 2012. Induced pluripotent stem cells from patients with Huntington's Disease show CAG-repeat-expansion-associated phenotypes. Cell Stem Cell 11(2), pp. 264-278. (10.1016/j.stem.2012.04.027)
Wray, S.et al. 2012. Creation of an open-access, mutation-defined fibroblast resource for neurological disease research. PLoS ONE 7(8), article number: e43099. (10.1371/journal.pone.0043099) pdf
Feyeux, M.et al. 2012. Early transcriptional changes linked to naturally occurring Huntington's disease mutations in neural derivatives of human embryonic stem cells. Human Molecular Genetics 21(17), pp. 3883-3895. (10.1093/hmg/dds216)
Patani, R.et al. 2011. Retinoid-independent motor neurogenesis from human embryonic stem cells reveals a medial columnar ground state. Nature Communications 2, article number: 214. (10.1038/ncomms1216)
Hollins, A.et al. 2011. Transplantation of differentiated human embryonic stem cells into a Huntington's Disease model: The challenges of generating neural cells suitable for replacement therapy in neurodegenerative disease. Cell Transplantation 20(4), pp. 563-564.