研究领域
Molecular and Synthetic Photobiology
Modern agriculture relies on crops that have been carefully tailored for environments by selective breeding. Phytochromes are attractive targets for crop improvement efforts aimed to enhance seed germination, seedling establishment, plant architecture and flowering time. The overall goal of this research is to understand the structural basis of both photosensory and regulatory functions of phytochromes from cyanobacteria to plants. Such basic knowledge will be leveraged for development of new strategies to alter the light responsiveness of agriculturally significant plant species. For example, our discovery of dominant photochemically uncoupled phytochrome alleles holds significant promise for crop improvement. Such plant-derived reagents may overcome environmental concerns while addressing the pressing need for increased food production on a finite amount of arable land needed to nourish the growing world population.
Research Interests
Molecular Photobiology
http://www.mcb.ucdavis.edu/faculty-labs/lagarias/
Research in my laboratory focuses on the phytochrome superfamily, light sensing biliproteins that are widespread in eukaryotes (plants, algae, fungi, oomycetes and diatoms) and prokaryotes (both photosynthetic and non-photosynthetic species). Phytochromes and cyanobacteriochromes are light-switches whose function depends on the light color. Plants, algae and cyanobacteria use these pigmented protein sensors to regulate photosynthesis-associated gene expression for optimum photosynthesis under the ambient light conditions and for regulation of growth, movement or reproduction to avoid suboptimal light conditions. Our studies utilize representative species from all lineages of oxygenic photosynthetic organisms, including cyanobacteria, green alga and plants. Mainly biochemical in nature, our research focuses on structure-function and molecular evolution of these optogenetic light sensors. Ongoing research seeks to leverage this knowledge for optimizing yield and agronomic performance of photosynthetic species used for food, fiber and energy production.
近期论文
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Rockwell, N.C. and Lagarias, J.C. (2017) Phytochrome diversification in cyanobacteria and eukaryotic algae. Current Opinion in Plant Biology in press, http://service.elsevier.com/app/answers/detail/a_id/5971
Rockwell, N.C., Martin, S.S., Li, F.-W., Mathews, S., and Lagarias, J.C. (2017) The phycocyanobilin chromophore of streptophyte algal phytochromes is synthesized by HY2. New Phytologist 214, 1145-1157.
Hu, W. and Lagarias, J.C. (2017) A tightly regulated genetic selection system with signaling-active alleles of phytochrome B. Plant Physiology 173, 366-375
Fushimi, K., Rockwell, N.C., Enomoto, G., Win, N.-N., Martin, S.S., Gan, F., Bryant, D.A., Ikeuchi, M., Lagarias, J.C., and Narikawa, R. (2016) Cyanobacteriochrome photoreceptors lacking the canonical Cys residue. Biochemistry 55(50), 6981-6995
Rockwell, N.C., Martin, S.S., and Lagarias, J.C. (2016) Identification of cyanobacteriochromes detecting far-red light. Biochemistry 55(28), 3907-3919
Chang, C.W., Gottlieb, S.M., Rockwell, N.C., Martin, S.S., Lagarias, J.C., and Larsen, D.S. (2016) Tracking the secondary photodynamics of the green/red cyanobacteriochrome RcaE from Fremyella diplosiphon. Chemical Physics Letters 644, 225-230
Lim, S., Yu, Q., Rockwell, N.C., Martin, S.S., Lagarias, J.C., and Ames, J.B. (2016) 1H, 13C, and 15N chemical shift assignments of cyanobacteriochrome NpR6012g4 in the green-absorbing photoproduct state. Biomolecular NMR Assignments 10(1), 157-161
Yu, Q., Lim, S., Rockwell, N.C., Martin, S.S., Lagarias, J.C., and Ames, J.B. (2016) 1H, 13C, and 15N chemical shift assignments of cyanobacteriochrome NpR6012g4 in the red-absorbing dark state. Biomolecular NMR Assignments 10(1), 139-142
Jones MA, Hu W, Litthauer S, Lagarias JC and Harmer SL (2015) A constitutively active allele of phytochrome B maintains circadian robustness in in the absence of light and photosynthetic inputs. Plant Physiology 169(1), 814-825
Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, Rockwell NC, Martin SS, Lagarias JC and Isacoff EY (2015) Photoactivatable Genetically-Encoded Calcium Indicators for Targeted Neuronal Imaging. Nature Methods 12(9), 852-858
Rockwell NC, Martin SS, Lim S, Lagarias JC and Ames JB (2015) Characterization of red/green cyanobacteriochrome NpR6012g4 by solution NMR spectroscopy: A hydrophobic pocket for the C15-E,anti chromophore in the photoproduct. Biochemistry, 54(24), 3772-3783
Rockwell NC, Martin SS, Lim S, Lagarias JC and Ames JB (2015) Characterization of red/green cyanobacteriochrome NpR6012g4 by solution NMR spectroscopy: A protonated bilin chromophore in both photostates. Biochemistry 54(16), 2581-2600
Rockwell NC, Martin SS, and Lagarias JC (2015) Identification of DXCF Cyanobacteriochrome Lineages with Predictable Photocycles. Photochem. Photobiol. Sci. 14(5), 929-941.
Gottlieb SM, Kim PW, Chang C-W, Hanke S, Hayer R, Rockwell NC, Martin SS, Lagarias JC and Larsen DS (2015) Conservation and diversity in the primary forward photodynamics of red/green cyanobacteriochromes. Biochemistry 54(4), 1028-1042
Rockwell NC, Martin SS, Gan F, Bryant DA, and Lagarias JC (2015) NpR3784 is the prototype for a distinctive group of red/green cyanobacteriochromes using alternative Phe residues for photoproduct tuning. Photochem. Photobiol. Sci. 14(2), 258 - 269
Rockwell NC, Lagarias JC and Bhattacharya D (2014) Primary endosymbiosis and the evolution of light and oxygen sensing in photosynthetic eukaryotes. Front. Ecol. Evol. 2, doi:10.3389/fevo.2014.00066
Duanmu* D, Bachy* C, Sudek S, Wong C-H, Jimenez V, Rockwell NC, Martin SS, Ngan CY, Reistetter E, van Baren MJ, Price DC, Wei C-L, Reyes-Prieto A, Lagarias JC and Worden AZ (2014) Marine algae and land plants share conserved phytochrome signaling systems. Proc. Natl. Acad. Sci. USA 111(44), 5827–15832 (*co-first authors)
Gan F, Zhang S, Rockwell NC, Martin SS, Lagarias JC and Bryant DA (2014) Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light. Science 345(6202), 1312-1317
Chen*, A, Li*, C, Hu*, W, Lau, MY, Lin, H, Rockwell, NC, Martin, SS, Jernstedt, JA Lagarias, JC and Dubcovsky, J (2014) PHYTOCHROME C plays a major role in the acceleration of wheat flowering under long day photoperiod. Proc. Natl. Acad. Sci. USA 111(28), 10037-10044 (*co-first authors)
Rockwell NC, Duanmu D, Martin SS. Bachy C, Price DC, Bhattacharya D, Worden AZ and Lagarias JC Eukaryotic Algal Phytochromes Span the Visible Spectrum (2014) Proc. Natl. Acad. Sci. USA 111(10), 3871-3876
Lim S, Rockwell, NC, Martin, SS, Dallas, JL, Lagarias, JC and Ames, JB (2014) Photoconversion changes bilin chromophore conjugation and protein secondary structure in the violet/orange cyanobacteriochrome NpF2164g3. Photochem. Photobiol. Sci. 13(6), 951-962
Rockwell, NC, Martin, SS, Gulevich, AG and Lagarias, JC (2014) Conserved phenylalanine residues are required for blue shifting of cyanobacteriochrome photoproducts, Biochemistry 53(19), 3118-3130
Kim, PW, Rockwell, NC, Martin, SS, Lagarias, JC and Larsen, DS (2014) Dynamic Inhomogeneity in the Cyanobacterial Phytochrome Cph1. Biochemistry 53(17), 2818-2826.
Hirose Y, Rockwell NC, Martin SS, Narikawa R, Inomata K, Lagarias JC and Ikeuchi M (2013) Green/red cyanobacteriochromes regulate complementary chromatic acclimation via a protochromic photocycle, Proc. Natl. Acad. Sci. USA 110(13), 4974-4979
Duanmu D, Casero D, Dent RM, Gallaher S, Yang W, Rockwell NC, Martin SS, Pellegrini M, Niyogi KK, Merchant SS, Grossman AR and Lagarias JC Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival. (2013) Proc. Natl. Acad. Sci. (USA) 110, 3621-3626
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