个人简介

Appointed to Section of Molecular and Cellular Biology faculty, UC-Davis (1996) Appointed to Chemistry faculty, UC Davis (1996) NIH Postdoctoral fellow, University of Wisconsin-Madison (1992-1996) Linus Pauling Prize, American Crystallographic Association (1991) Ph.D., Biophysics, Purdue University (1992) B.S., Biochemistry, Purdue University (1987)

研究领域

Mechanistic Structural Biochemistry

X-ray crystallography is one of the most powerful tools to examine protein structure at atomic detail. My laboratory uses single crystal x-ray diffraction techniques to determine the three-dimensional structure of biologically important proteins. Knowledge of the atomic resolution structure provides tremendous insight into understanding how the protein functions. Hypotheses on structure - function relationships can be drawn from the crystal structure, which can be tested using crystallography and other biophysical and biochemical techniques. Currently, the laboratory is focusing on enzymes in the biologically important pathways of; sulfate activation, sialic acid transfer, and chromophore biosynthesis.

近期论文

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Kohler, A.C., Chen, L.H., Hurlburt, N., Salvucci, A., Schwessinger, B., Fisher, A.J., Stergiopoulos, I. (2016). Structural analysis of an Avr4 effector ortholog offers insight into chitin-binding and recognition by the Cf-4 receptor. Plant Cell. 28:1945-1965. [PDF Reprint] Matthews, M. M., *Thomas, J. M., Zheng, Y., Tran, K., Phelps, K. J., Scott, A. I., Havel, J., Fisher, A. J., and Beal, P. A. (2016). Structures of human ADAR2 bound to dsRNA reveal base-flipping mechanism and basis for site selectivity. Nat. Struct. Mol. Biol. 23:426-433. [PDF Reprint] (*Authors contributed equally) Erickson, A. I., Sarsam, R. D., and Fisher, A. J. (2015) Structures of Mycobacterium tuberculosis CysQ, with substrate and products bound. Biochemistry 54:6830-6841. [PDF Reprint] Phelps, K., Ibarra-Soza, J. M., Zheng, Y., Fisher, A. J., Beal, P. A. (2014). Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA. ACS Chemical Biology, 9:1780-1787. [PDF Reprint] Erickson, A. I., Sarsam, R. D., and Fisher, A. J. (2014) Expression, purification and preliminary crystallographic analysis of Mycobacterium tuberculosis CysQ, a phosphatase involved in sulfur metabolism. Acta Cryst. F70:747-749 [PDF Reprint] Huynh, N., Aye, A., Li, Y., Yu, H., Cao, H., Tiwari, V. K., Shin, D.-W., Chen, X., and Fisher, A. J. (2013). Structural basis for substrate specificity and mechanism of N-acetyl-D-neuraminic acid lyase from Pasteurella multocida. Biochemistry 52:8570-8579. [PDF Reprint] Addington, T. A., Mertz, R. W., Siegel, J. B., Thompson, J. M., Fisher, A. J., Filkov, V., Fleischman, N., Suen, A., Zhang, C., Michael D. Toney, M. D. (2013). JANUS: Prediction and Ranking of Mutations Required for Functional Interconversion of Enzymes. J. Mol. Biol. 425:1378-1389. [PDF Reprint] Sugiarto, G., Lau, K., Qu, J., Li, Y., Lim, S., Mu, S., Ames, J. B., Fisher, A. J. , and Chen, X. (2012). A Sialyltransferase Mutant with Decreased Donor Hydrolysis and Reduced Sialidase Activities for Direct Sialylating Lewisx. ACS Chem. Biol. 7:1232-1240. [PDF Reprint] Griswold, W. R., Castro, J. N., Fisher, A. J., and Toney, M. D. (2012). Ground-State Electronic Destabilization via Hyperconjugation in Aspartate Aminotransferase. J. Am. Chem. Soc. 134:8436-8438. [PDF Reprint] Griswold, W. R., Fisher, A. J., and Toney, M. D. (2011). Crystal Structure of Aspartate Aminotransferase Reconstituted with 1-Deazapyridoxal 5’-Phosphate: Internal Aldimine and Stable L-Aspartate External Aldimine. Biochemistry 50:5918-5924. [PDF Reprint] Kohler, A. C., Gae, D. D., Richley M. A., Stoll S., Gunn, A., Sunghyuk Lim S., Martin S. S., Tzanko I. Doukov T. I., Britt, R. D., Ames, J. B., Lagarias, J. C., and Fisher, A. J. (2010). Structural basis for hydration dynamics in radical stabilization of bilin reductase mutants. Biochemistry 49:6206-6218. [PDF Reprint] Stoll, S., Gunn, A., Brynda, M., Sughrue, W., Kohler, A. C., Ozarowski, A., Fisher, A. J., Lagarias, J. C., Britt, R. D. (2009). The structure of the biliverdin radical intermediate in phycocyanobilin:ferredoxin oxidoreductase identified by high-field EPR and DFT. J. Am. Chem. Soc. 131:1986-1995. [PDF Reprint] Gay, S. C., Segel, I. H., and Fisher, A. J. (2009). Crystal structure of the two-domain, hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity. Acta Crystallogr. D Biol. Crystallogr. 65:1021-1031. [PDF Reprint] Yu, Z, Lemongello, D., Segel, I. H., and Fisher, A. J. (2008). Crystal structure of Saccharomyces cerevisiae 3'-Phosphoadenosine-5'-phosphosulfate Reductase complexed with adenosine 3',5'-bisphosphate. Biochemistry. 47:12777–12786. [PDF Reprint]