Loughna, Siobhan - University of Nottingham

个人简介

Graduated from the University of Sheffield with a BSc (Hons) in Genetics (1989); PhD in Developmental Biology (1994) from the Royal Postgraduate Medical School, University of London; British Heart Foundation funded postdoctoral researcher, Developmental Biology Unit, Division of Cell and Molecular Biology, Institute of Child Health in London (1994-1997); Postdoctoral Fellow, Department of Internal Medicine, Cardiology Laboratories at The University of Texas Southwestern Medical Center, Dallas, Texas, USA (1998-2001); Lecturer in Anatomy and Developmental Biology, University of Nottingham (2001-present).

研究领域

Our research interests are to provide insights into how the heart forms during early stages of cardiogenesis. In particular, current interests are focused on the role sarcomeric thick or thin filament structural proteins play in the early developing heart. Heart defects of particular interest in the laboratory are those associated with the developing atrial septa, chamber specification, normal electrical activation of the heart and the formation of the conduction system. Congenital heart defects are relatively common (approximately 0.8% of the population), with most cases having an unknown cause. Our research group uses the chick as a model organism (both in ovo and in vitro), and morpholino technology to perform gene-specific knockdown. A range of developmental, cell and molecular biology techniques are employed to decipher the expression of the genes of interest and the abnormalities seen upon knockdown. Further, functional studies are performed to provide insights into what role the genes play in the heart in order to explain how defects form. In addition, in order to analyse the morphological effects on post-loaded hearts, we have recently optimised banding of the outflow tract of the developing chick heart, with harvesting performed at different stages of development. Dr Loughna runs an active laboratory and currently has three PhD students and one part-time technician.

近期论文

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ENGLAND J, PANG KL, PARNALL M, HAIG MI and LOUGHNA S, 2016. Cardiac troponin T is necessary for normal development in the embryonic chick heart. Journal of anatomy. 229(3), 436-49 TUSHAR K. GHOSH, JAVIER T. GRANADOS-RIVERON, SARAH BUXTON, KERRY SETCHFIELD, SIOBHAN LOUGHNA AND J. DAVID BROOK, 2014. Studies of Genes Involved in Congenital Heart Disease J. Cardiovasc. Dev. Dis. 1(1), 134-145 ENGLAND J and LOUGHNA S, 2013. Heavy and light roles: myosin in the morphogenesis of the heart. Cellular and molecular life sciences : CMLS. 70(7), 1221-39 RUTLAND, C.S., POLO-PARADA, L., EHLER, E., ALIBHAI, A., THORPE, A., SUREN, S., EMES, R.D., PATEL, B. and LOUGHNA, S., 2011. Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart Development. 138(18), 3955-3966 WALSH, R., RUTLAND, C., THOMAS, R. and LOUGHNA, S., 2010. Cardiomyopathy: a systematic review of disease-causing mutations in myosin heavy chain 7 and their phenotypic manifestations Cardiology. 115(1), 49-60 RUTLAND, C., WARNER, L., THORPE, A., ALIBHAI, A., ROBINSON, T., SHAW, B., LAYFIELD, R., BROOK, J.D. and LOUGHNA, S., 2009. Knockdown of alpha myosin heavy chain disrupts the cytoskeleton and leads to multiple defects during chick cardiogenesis Journal of Anatomy. 214(6), 905-915 CHING, Y.-H., GHOSH, T.K., CROSS, S.J., PACKHAM, E.A., HONEYMAN, L., LOUGHNA, S., ROBINSON, T.E., DEARLOVE, A.M., RIBAS, G., BONSER, A.J., THOMAS, N.R., SCOTTER, A.J., CAVES, L.S.D., TYRRELL, G.P., NEWBURY-ECOB, R.A., MUNNICH, A., BONNET, D. and BROOK, J.D., 2005. Mutation in myosin heavy chain 6 causes atrial septal defect Nature Genetics. 37(4), 423-428 SATO, T N, LOUGHNA, S, DAVIS, E C, VISCONTI, R P and RICHARDSON, C D, 2002. Selective functions of angiopoietins and vascular endothelial growth factor on blood vessels: the concept of "vascular domain". Cold Spring Harbor Symposia on Quantitative Biology. 67, 171-80 LOUGHNA,S., 2002. Vascular development. In: Mouse development: patterning, morphogenesis and organogenesis. Academic Press, Incorporated, Orlando, Florida, USA. LOUGHNA, S. and SATO, T. N., 2001. A combinatorial role of angiopoietin-1 and orphan receptor TIE1 pathways in establishing vascular polarity during angiogenesis Molecular Cell. 7(1), 233-239 LOUGHNA, S. and SATO, T. N., 2001. Angiopoietin and Tie signaling pathways in vascular development Matrix Biology. 20(5-6), 319-325 MOTOIKE, T, LOUGHNA, S, PERENS, E, ROMAN, B L, LIAO, W, CHAU, T C, RICHARDSON, C D, KAWATE, T, KUNO, J, WEINSTEIN, B M, STAINIER, D Y and SATO, T N, 2000. Universal GFP reporter for the study of vascular development. Genesis. 28(2), 75-81 LOUGHNA, S, YUAN, H T and WOOLF, A S, 1998. Effects of oxygen on vascular patterning in Tie1/LacZ metanephric kidneys in vitro. Biochemical and Biophysical Research Communications. 247(2), 361-6 WOOLF, A S and LOUGHNA, S, 1998. Origin of glomerular capillaries: is the verdict in? Experimental nephrology.. 6(1), 17-21 LOUGHNA, S, HARDMAN, P, LANDELS, E, JUSSILA, L, ALITALO, K and WOOLF, A S, 1997. A molecular and genetic analysis of renalglomerular capillary development. Angiogenesis. 1(1), 84-101 LOUGHNA, S, LANDELS, E and WOOLF, A S, 1996. Growth factor control of developing kidney endothelial cells. Experimental nephrology.. 4(2), 112-8 LOUGHNA, S, BENNETT, P and MOORE, G, 1995. Molecular analysis of the expression of transthyretin in intestine and liver from trisomy 18 fetuses. Human Genetics. 95(1), 89-95 HENDERSON, D J, SHERMAN, L S, LOUGHNA, S C, BENNETT, P R and MOORE, G E, 1994. Early embryonic failure associated with uniparental disomy for human chromosome 21. Human Molecular Genetics. 3(8), 1373-6 NEWTON, R, STANIER, P, LOUGHNA, S, HENDERSON, D J, FORBES, S A, FARRALL, M, JENSSON, O and MOORE, G E, 1994. Linkage analysis of 62 X-chromosomal loci excludes the X chromosome in an Icelandic family showing apparent X-linked recessive inheritance of neural tube defects. Clinical genetics.. 45(5), 241-9 LOUGHNA, S, BENNETT, P, GAU, G, NICOLAIDES, K, BLUNT, S and MOORE, G, 1993. Overexpression of esterase D in kidney from trisomy 13 fetuses. American Journal of Human Genetics. 53(4), 810-6 BENNETT, P, VAUGHAN, J, HENDERSON, D, LOUGHNA, S and MOORE, G, 1992. Association between confined placental trisomy, fetal uniparental disomy, and early intrauterine growth retardation. Lancet. 340(8830), 1284-5