Dawson, Simon - University of Nottingham

研究领域

Research Interests Research interests include the role of the gankyrin oncoprotein in hepatocellular carcinogenesis; the roll of ubiquitin-protein ligase enzymes (E3s) in cell biology particularly the NEDD4-family of E3s within intracellular protein trafficking, signalling and the identification of potential substrates thereof; ubiquitin-like proteins. Techniques • Protein-protein interaction analysis - two-hybrid screening and analysis via 'classical' and SOS/ras yeast and bacterial systems, co-immunoprecipitation, fluorescence microscopy • Cell tissue culture • General molecular biology - PCR, in vitro mutagenesis, cloning etc • Recombinant protein expression via bacterial and Pichia pastoris systems • Reporter gene analysis

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

REZVANI, K, BAALMAN, K, TENG, Y, MEE, M.P., DAWSON, S.P., WANG, H, DE BIASI, M and MAYER, R.J., 2012. Proteasomal degradation of the metabotropic glutamate receptor 1α is mediated by Homer-3 via the proteasomal S8 ATPase: Signal transduction and synaptic transmission Journal of Neurochemistry. 122(1), 24-37 DAWSON, S.P., 2008. Hepatocellular carcinoma and the ubiquitin-proteasome system Biochimica et Biophysica Acta: Molecular Basis of Disease. 1782(12), 775-784 MARX, FP, SOEHN, AS, BERG, D, MELLE, C, SCHIESLING, C, LANG, M, KAUTZMANN, S, STRAUSS, KM, FRANCK, T, ENGELENDER, S, PAHNKE, J, DAWSON, S, VON EGGELING, F, SCHULZ, JB, RIESS, O and KRUGER, R, 2007. The proteasomal subunit S6 ATPase is a novel synphilin-1 interacting protein--implications for Parkinson's disease. FASEB Journal. 21(8), 1759-67 DAWSON, S., HIGASHITSUJI, H., WILKINSON, A.J., FUJITA, J. and MAYER, R.J., 2006. Gankyrin: a new oncoprotein and regulator of pRb and p53 Trends in Cell Biology. 16(5), 229-233 HIGASHITSUJI, H., HIGASHITSUJI, H., ITOH, K., SAKURAI, T., NAGAO, T., SUMITOMO, H., MASUDA, T., DAWSON, S., SHIMADA, Y., MAYER, R. J. and FUJITA, J, 2005. The oncoprotein gankyrin binds to MDM2/HDM2, enhancing ubiquitylation and degradation of p53 Cancer Cell. 8(1), 75-87 KRZYWDA, S., BRZOZOWSKI, A. M., HIGASHITSUJI, H., FUJITA, J., WELCHMAN, R., DAWSON, S., MAYER, R. J. and WILKINSON, A. J., 2004. The crystal structure of gankyrin, an oncoprotein found in complexes with cyclin-dependent kinase 4, a 19 S proteasomal ATPase regulator, and the tumor suppressors Rb and p53 Journal of Biological Chemistry. 279(2), 1541-1545 APCHER, G-S, MAITLAND, J, DAWSON, S, SHEPPARD, P and MAYER, R, 2004. The alpha4 and alpha7 subunits and assembly of the 20S proteasome. FEBS Letters. 569(1-3), 211-216 NAGAO, T., HIGASHITSUJI, H., NONOGUCHI, K., SAKURAI, T., DAWSON, S., MAYER, R. J., ITOH, K. and FUJITA, J., 2003. MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity Journal of Biological Chemistry. 278(12), 10668-10674 REZVANI, K, MEE, M, DAWSON, S, MCILHINNEY, J, FUJITA, J and MAYER, R J, 2003. Proteasomal interactors control activities as diverse as the cell cycle and glutaminergic neurotransmission. Biochemical Society Transactions. 31(2), 470-3 DAWSON, S., APCHER, S., MEE, M., HIGASHITSUJI, H., BAKER, R., UHLE, S., DUBIEL, W., FUJITA, J. and MAYER, R. J., 2002. Gankyrin is an ankyrin-repeat oncoprotein that interacts with CDK4 kinase and the S6 ATPase of the 26 S proteasome Journal of Biological Chemistry. 277(13), 10893-10902 DOHERTY, F. J., DAWSON, S. and MAYER, R. J., 2002. The ubiquitin-proteasome pathway of intracellular proteolysis Essays in Biochemistry. VOL 38, 51-64 LAYFIELD, R, TOOTH, D, LANDON, M, DAWSON, S, MAYER, J and ALBAN, A, 2001. Purification of poly-ubiquitinated proteins by S5a-affinity chromatography. Proteomics. 1(6), 773-7