Cockayne, Alan - University of Nottingham

研究领域

My main current research interest is in metal ion uptake systems in staphylococci and Clostridium difficile. Although such systems have been well characterised in many Gram negative pathogens those in many Gram positives still remain poorly defined. I am particularly interested in ABC type transporters and their functions and the regulation of their expression in response to metal ion limitation. I also have a more general interest in microbial pathogenicity and have previously worked with a variety of other organisms including Candida albicans, spirochetes and Helicobacter pylori. Techniques: General molecular biology techniques, protein expression and purification, microbial cell fractionation and radiolabelling, tissue culture and use of Galleria mellonella as a non-vertebrate infection model.

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

KELLY ML, NG YK, CARTMAN ST, COLLERY MM, COCKAYNE A and MINTON NP, 2016. Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection. Anaerobe. 39, 51-3 COLLERY MM, KUEHNE SA, MCBRIDE SM, KELLY ML, MONOT M, COCKAYNE A, DUPUY B and MINTON NP, 2016. What's a SNP between friends: The influence of single nucleotide polymorphisms on virulence and phenotypes of Clostridium difficile strain 630 and derivatives. Virulence. 0 (In Press.) HARVEY D, BARDELANG P, GOODACRE SL, COCKAYNE A and THOMAS NR, 2016. Antibiotic Spider Silk: Site-Specific Functionalization of Recombinant Spider Silk Using "Click" Chemistry. Advanced materials (Deerfield Beach, Fla.). (In Press.) OKOLIE CE, WOOLDRIDGE KG, TURNER DP, COCKAYNE A and JAMES R, 2015. Development of a heptaplex PCR assay for identification of Staphylococcus aureus and CoNS with simultaneous detection of virulence and antibiotic resistance genes. BMC microbiology. 15, 157 MONAGHAN TM, COCKAYNE A and MAHIDA YR, 2015. Pathogenesis of Clostridium difficile Infection and Its Potential Role in Inflammatory Bowel Disease. Inflammatory bowel diseases. 21(8), 1957-66 OKOLIE CE, WOOLDRIDGE KG, TURNER DP, COCKAYNE A and JAMES R, 2015. Development of a new pentaplex real-time PCR assay for the identification of poly-microbial specimens containing Staphylococcus aureus and other staphylococci, with simultaneous detection of staphylococcal virulence and methicillin resistance markers. Molecular and cellular probes. 29(3), 144-50 HOOK, ANDREW L., CHANG, CHIEN-YI, YANG, JING, LUCKETT, JENI, COCKAYNE, ALAN, ATKINSON, STEVE, MEI, YING, BAYSTON, ROGER, IRVINE, DEREK J., LANGER, ROBERT, ANDERSON, DANIEL G., WILLIAMS, PAUL, DAVIES, MARTYN C. and ALEXANDER, MORGAN R., 2014. Combinatorial discovery of polymers resistant to bacterial attachment (vol 30, pg 868, 2012) NATURE BIOTECHNOLOGY. 32(6), 592-592 MURRAY EJ, CROWLEY RC, TRUMAN A, CLARKE SR, COTTAM JA, JADHAV GP, STEELE VR, O'SHEA P, LINDHOLM C, COCKAYNE A, CHHABRA SR, CHAN WC and WILLIAMS P, 2014. Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors. Journal of medicinal chemistry. 57(6), 2813-9 KUEHNE SA, COLLERY MM, KELLY ML, CARTMAN ST, COCKAYNE A and MINTON NP, 2013. Importance of Toxin A, Toxin B, and CDT in Virulence of an Epidemic Clostridium difficile Strain. The Journal of infectious diseases. HOOK, A.L., CHANG C.-Y., YANG, J., LUCKETT, J., COCKAYNE, A., ATKINSON, S., MEI, Y., BAYSTON, R., IRVINE, D.J., LANGER, R., ANDERSON, D.G., WILLIAMS, P., DAVIES, M.C. and ALEXANDER, M.R., 2012. Combinatorial discovery of polymers resistant to bacterial attachment Nature Biotechnology. 30(9), 868-875 JOHNSON, M., SENGUPTA, M., PURVES, J., TARRANT, E., WILLIAMS, P.H., COCKAYNE, A., MUTHAIYAN, A., STEPHENSON, R., LEDALA, N., WILKINSON, B.J., JAYASWAL, R.K. and MORRISSEY, J.A., 2011. Fur is required for the activation of virulence gene expression through the induction of the sae regulatory system in Staphylococcus aureus International Journal of Medical Microbiology. 301(1), 44-52 KUEHNE, S.A., CARTMAN, S.T., HEAP, J.T., KELLY, M.L., COCKAYNE, A. and MINTON, N.P., 2010. The role of toxin A and toxin B in Clostridium difficile infection Nature. 467(7316), 711-713 CARTMAN, S.T., HEAP, J.T., KUEHNE, S.A., COCKAYNE, A. and MINTON, N.P., 2010. The emergence of 'hypervirulence' in Clostridium difficile International Journal of Medical Microbiology. 300(6), 387-395 PURVES, J., COCKAYNE, A., MOODY, P.C.E. and MORRISSEY, J.A., 2010. Comparison of the regulation, metabolic functions, and roles in virulence of the glyceraldehyde-3-phosphate dehydrogenase homologues gapA and gapB in Staphylococcus aureus Infection and Immunity. 78(12), 5223-5232 JOHNSON, M., COCKAYNE, A. and MORRISSEY, J.A., 2008. Iron-regulated biofilm formation in Staphylococcus aureus Newman requires ica and the secreted protein Emp Infection and Immunity. 76(4), 1756-1765 SHARP, K.H., SCHNEIDER, S., COCKAYNE, A. and PAOLI, M., 2007. Crystal structure of the heme-IsdC complex, the central conduit of the Isd iron/heme uptake system in Staphylococcus aureus. Journal of Biological Chemistry. 282(14), 10625-10631 PASPARAKIS, G., COCKAYNE, A. and ALEXANDER, C., 2007. Control of bacterial aggregation by thermoresponsive glycopolymers Journal of the American Chemical Society. 129(36), 11014-11015 QAZI, S., MIDDLETON, B., MUHARRAM, S.H., COCKAYNE, A., HILL, P., O'SHEA, P., CHHABRA, S.R., CAMARA, M. and WILLIAMS, P., 2006. N-acylhomoserine lactones antagonize virulence gene expression and quorum sensing in staphylococcus aureus Infection and Immunity. 74(2), 910-919 AUSTIN, A.S., JUDGE, H.M., ROBINS, R.A., COCKAYNE, A. and MAHIDA, Y.R., 2005. Responses to free lipopolysaccharide and Escherichia coli by normal human intestinal macrophages, following their migration out of the lamina propria Scandinavian Journal of Immunology. 61(6), 575-584 JOHNSON, M., COCKAYNE, A., WILLIAMS, P.H. and MORRISSEY, J.A., 2005. Iron-responsive regulation of biofilm formation in Staphylococcus aureus involves fur-dependent and fur-independent mechanisms Journal of Bacteriology. 187(23), 8211-8215 MORRISSEY, J.A., COCKAYNE, A., BRUMMELL, K. and WILLIAMS, P., 2004. The staphylococcal ferritins are differentially regulated in response to iron and manganese and via PerR and fur Infection and Immunity. 72(2), 972-979 SCOTT, R.J., LIAN, L.Y., MUHARRAM, S.H., COCKAYNE, A., WOOD, S.J., BYCROFT, B.W., WILLIAMS, P. and CHAN, W.C., 2003. Side-chain-to-tail thiolactone peptide inhibitors of the staphylococcal quorum-sensing system Bioorganic and Medicinal Chemistry Letters. 13(15), 2449-53 SOMERVILLE, G.A., COCKAYNE, A., DURR, M., PESCHEL, A., OTTO, M. and MUSSER, J.M., 2003. Synthesis and deformylation of Staphylococcus aureus δ-toxin are linked to tricarboxylic acid cycle activity Journal of Bacteriology. 185(22), 6686-6694 COOKSLEY, C., JENKS, P. J., GREEN, A., COCKAYNE, A., LOGAN, R. P. H. and HARDIE, K. R., 2003. NapA protects Helicobacter pylori from oxidative stress damage, and its production is influenced by the ferric uptake regulator Journal of Medical Microbiology. VOL 52(PART 6), 461-469 MORRISSEY, J.A., COCKAYNE, A., HAMMACOTT, J., BISHOP, K., DENMAN-JOHNSON, A., HILL, P.J. and WILLIAMS, P., 2002. Conservation, surface exposure, and in vivo expression of the Frp family of iron-regulated cell wall proteins in Staphylococcus aureus. Infection and Immunity. 70(5), 2399-2407