Aylott, Jonathan - University of Nottingham

个人简介

I gained my degree and PhD from the University of East Anglia. I then undertook a Postdoctoral Fellowship in Raoul Kopelman's laboratory at the University of Michigan. In 2000, I returned to the UK to take up a Lectureship in Analytical Science at the Department of Chemistry, University of Hull. In 2004 I was appointed Lecturer in Analytical Bioscience in the School of Pharmacy at Nottingham.Teaching

研究领域

My research interests focus on the design, development and implementation of miniaturized analytical devices. Such devices can then be applied to measuring biological samples in-situ and in real-time, generating a better understanding of disease states. Optical nanosensors are the primary focus of this research and utilise the sensitivity of fluorescence to make quantitative measurements inside living cells. The nanosensor devices are typically 50 nm in diameter and small enough to be inserted into living cells with a minimum of physical perturbation. Nanosensors enable real-time changes in small molecule concentrations in single cells to be made. They exhibit advantages over widely used fluorescence dye based methods because the nanosensor matrix imparts two key benefits: 1. Protection of the sensing component from interfering species within the intracellular environment 2. Protection of the intracellular environment from any toxic effects of the sensing component. Nanosensors capable of measuring glucose, oxygen, calcium, zinc and pH have been prepared and work is ongoing to widen the range of analytes that can be detected using this technology. An additional attractive feature of nanosensors is that they can be imaged and quantified using standard technologies, e.g., confocal microscopy, utilised in life science research. Further areas of research include developing strategies for introducing nanosensors to cells, the goal being a generic 'soft' chemical method with which to routinely deliver nanosensors to large numbers of living cells in a controllable manner. And to develop optical methods capable of resolving individual nanosensors, thereby overcoming Abbe's diffraction limit, and offering the possibility of an optical nanoscope.

近期论文

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TRES, FRANCESCO, TREACHER, KEVIN, BOOTH, JONATHAN, HUGHES, LESLIE P., WREN, STEPHEN A. C., AYLOTT, JONATHAN W. and BURLEY, JONATHAN C., 2016. Indomethacin-Kollidon VA64 Extrudates: A Mechanistic Study of pH-Dependent Controlled Release MOLECULAR PHARMACEUTICS. 13(3), 1166-1175 LIGHTFOOT JW, CHAUHAN VM, AYLOTT JW and R?DELSPERGER C, 2016. Comparative transcriptomics of the nematode gut identifies global shifts in feeding mode and pathogen susceptibility. BMC research notes. 9, 142 BRIDGE, JACK C., AYLOTT, JONATHAN W., BRIGHTLING, CHRISTOPHER E., GHAEMMAGHAMI, AMIR M., KNOX, ALAN J., LEWIS, MARK P., ROSE, FELICITY R. A. J. and MORRIS, GAVIN E., 2015. Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. ORSI, GIANNI, DE MARIA, CARMELO, MONTEMURRO, FRANCESCA, CHAUHAN, VEEREN M., AYLOTT, JONATHAN W. and VOZZI, GIOVANNI, 2015. Combining Inkjet Printing and Sol-Gel Chemistry for Making pH-Sensitive Surfaces CURRENT TOPICS IN MEDICINAL CHEMISTRY. 15(3), 271-278 LAVADO, ANDREA S., CHAUHAN, VEEREN M., ZEN, AMER ALHAJ, GIUNTINI, FRANCESCA, JONES, D. RHODRI E., BOYLE, ROSS W., BEEBY, ANDREW, CHAN, WENG C. and AYLOTT, JONATHAN W., 2015. Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles NANOSCALE. 7(34), 14525-14531 TRES, FRANCESCO, PATIENT, JAMIE D., WILLIAMS, PHILIP M., TREACHER, KEVIN, BOOTH, JONATHAN, HUGHES, LESLIE P., WREN, STEPHEN A. C., AYLOTT, JONATHAN W. and BURLEY, JONATHAN C., 2015. Monitoring the Dissolution Mechanisms of Amorphous Bicalutamide Solid Dispersions via Real-Time Raman Mapping MOLECULAR PHARMACEUTICS. 12(5), 1512-1522 TIRELLA, ANNALISA, LA MARCA, MARGHERITA, BRACE, LEIGH-ANNE, MATTEI, GIORGIO, AYLOTT, JONATHAN W. and AHLUWALIA, ARTI, 2015. Nano-in-Micro Self-Reporting Hydrogel Constructs JOURNAL OF BIOMEDICAL NANOTECHNOLOGY. 11(8), 1451-1460 MARQUES, LEONEL, CHEN, XINYONG, ROBERTS, CLIVE J., CLARK, MATTHEW and AYLOTT, JONATHAN W., 2015. Facile approach to generating polymeric nanoarrays containing populations of nanoparticles MICRO & NANO LETTERS. 10(8), 378-383 HTWE, SU SU, HARRINGTON, HELEN, KNOX, ALAN, ROSE, FELICITY, AYLOTT, JONATHAN, HAYCOCK, JOHN W. and GHAEMMAGHAMI, AMIR M., 2015. Investigating NF-kappa B signaling in lung fibroblasts in 2D and 3D culture systems RESPIRATORY RESEARCH. 16, 144 TRES, FRANCESCO, COOMBES, STEVEN R., PHILLIPS, ANDREW R., HUGHES, LESLIE P., WREN, STEPHEN A. C., AYLOTT, JONATHAN W. and BURLEY, JONATHAN C., 2015. Investigating the Dissolution Performance of Amorphous Solid Dispersions Using Magnetic Resonance Imaging and Proton NMR MOLECULES. 20(9), 16404-16418 SMITH, RICHARD J., COTA, FERNANDO PEREZ, MARQUES, LEONEL, CHEN, XUESHENG, ARCA, AHMET, WEBB, KEVIN, AYLOTT, JONATHON, SOMEKH, MICHEAL G. and CLARK, MATT, 2015. Optically excited nanoscale ultrasonic transducers JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA. 137(1), 219-227 TRES, FRANCESCO, TREACHER, KEVIN, BOOTH, JONATHAN, HUGHES, LES P., WREN, STEPHEN A. C., AYLOTT, JONATHAN W. and BURLEY, JONATHAN C., 2014. Real time Raman imaging to understand dissolution performance of amorphous solid dispersions JOURNAL OF CONTROLLED RELEASE. 188, 53-60 HARRINGTON, HELEN, CATO, PAUL, SALAZAR, FABIAN, WILKINSON, MALCOLM, KNOX, ALAN, HAYCOCK, JOHN W., ROSE, FELICITY, AYLOTT, JON W. and GHAEMMAGHAMI, AMIR M., 2014. Immunocompetent 3D Model of Human Upper Airway for Disease Modeling and In Vitro Drug Evaluation MOLECULAR PHARMACEUTICS. 11(7), 2082-2091 MORRIS, G. E., BRIDGE, J. C., ELTBOLI, O. M. I., KNOX, A. J., AYLOTT, J. W., BRIGHTLING, C. E., GHAEMMAGHAMI, A. M. and ROSE, F. R. A. J., 2014. Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY. 307(1), L38-L47 GIUNTINI, F., CHAUHAN, V. M., AYLOTT, J. W., ROSSER, G. A., ATHANASIADIS, A., BEEBY, A., MACROBERT, A. J., BROWN, R. A. and BOYLE, R. W., 2014. Conjugatable water-soluble Pt(II) and Pd(II) porphyrin complexes: novel nano-and molecular probes for optical oxygen tension measurement in tissue engineering PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES. 13(7), 1039-1051 CHAUHAN, VEEREN M., HOPPER, RICHARD H., ALI, SYED Z., KING, EMMA M., UDREA, FLORIN, OXLEY, CHRIS H. and AYLOTT, JONATHAN W., 2014. Thermo-optical characterization of fluorescent rhodamine B based temperature-sensitive nanosensors using a CMOS MEMS micro-hotplate SENSORS AND ACTUATORS B-CHEMICAL. 192, 126-133 MORRIS, G. E., BRIDGE, J. C., BRACE, L. A., KNOX, A. J., AYLOTT, J. W., BRIGHTLING, C. E., GHAEMMAGHAMI, A. M. and ROSE, F. R. A. J., 2014. A novel electrospun biphasic scaffold provides optimal three-dimensional topography for in vitro co-culture of airway epithelial and fibroblast cells BIOFABRICATION. 6(3), ZEN, AMER ALHAJ, AYLOTT, JONATHAN W. and CHAN, WENG C., 2014. An appraisal of the Suzuki cross-coupling reaction for the synthesis of novel fluorescent coumarin derivatives TETRAHEDRON LETTERS. 55(40), 5521-5524 DESAI, ARPAN S., CHAUHAN, VEEREN M., JOHNSTON, ANGUS P. R., ESLER, TIM and AYLOTT, JONATHAN W., 2014. Fluorescent nanosensors for intracellular measurements: synthesis, characterization, calibration, and measurement FRONTIERS IN PHYSIOLOGY. 4, ABULATEEFEH, S.R., SPAIN, S.G., THURECHT, K.J., AYLOTT, J.W., CHAN, W.C., GARNETT, M.C. and ALEXANDER, C., 2013. Enhanced uptake of nanoparticle drug carriers via a thermoresponsive shell enhances cytotoxicity in a cancer cell line Biomaterials Science. 1(4), 434-442 YAA ASANTEWAA, JONATHAN AYLOTT, JONATHAN C BURLEY, NASHIRU BILLA and CLIVE J ROBERTS, 2013. Correlating Physicochemical Properties of Boronic Acid-Chitosan Conjugates to Glucose Adsorption Sensitivity Pharmaceutics. 5, 69-80 HARRINGTON, H. C., ROSE, F. R. A. J., REINWALD, Y., BUTTERY, L. D. K., GHAEMMAGHAMI, A. M. and AYLOTT, J. W., 2013. Electrospun PLGA fibre sheets incorporating fluorescent nanosensors: self-reporting scaffolds for application in tissue engineering ANALYTICAL METHODS. 5(1), 68-71 CHAUHAN, V.M., ORSI, G., BROWN, A., PRITCHARD, D.I. and AYLOTT, J.W., 2013. Mapping the pharyngeal and intestinal pH of Caenorhabditis elegans and real-time luminal pH oscillations using extended dynamic range pH-sensitive nanosensors ACS Nano. 7(6), 5577-5587 HELEN HARRINGTON, FELICITY RJ ROSE, JONATHAN W AYLOTT and AMIR M. GHAEMMAGHAMI, 2013. Self-reporting Scaffolds for 3-Dimensional Cell Culture. Journal of Visualized Experiments. 81, e50608 ABULATEEFEH, SAMER R., SPAIN, SEBASTIAN G., THURECHT, KRISTOFER J., AYLOTT, JONATHAN W., CHAN, WENG C., GARNETT, MARTIN C. and ALEXANDER, CAMERON, 2013. Enhanced uptake of nanoparticle drug carriers via a thermoresponsive shell enhances cytotoxicity in a cancer cell line BIOMATERIALS SCIENCE. 1(4), 434-442