Magoski, Neil S - Queen's University - Department of Biomedical and Molecular Sciences

个人简介

Ion channel modulation and the control of neuronal excitability. We study how the regulation of ion channel function initiates fundamental behaviours. Our focus is a group of neuroendocrine cells that trigger reproduction through a prolonged afterdischarge and release of peptide hormones. Electrophysiology, imaging, and molecular biology are used to examine the role of calcium and non-selective cation channels in controlling excitability and secretion. The work we undertake shows how neuronal and animal behaviour can be altered for extended periods following brief sensory input.

研究领域

We use electrophysiology, imaging, and molecular biology to investigate how changes to neuroendocrine cell excitability and peptide release control ovulation in the mollusc, Aplysia californica. This large, marine snail has been used for almost 50 years to study the cellular basis of learning and memory, motor pattern generation, defensive reactions, and reproduction, as well as ion channel modulation and function. Areas of research: • The control of excitability and secretion by voltage-gated Ca2+ channels and non-selective cation channels. • Gap junction biophysics and modulation, as well as the role of electrical synapses in shaping neuronal bursting. Aplysia bag cell neurons: • In Aplysia (pictured left), the bag cell neurons are found in two clusters in the nervous system and serve as command neurons for egg-laying behaviour. • These neuroendocrine cells can be examined either in the intact nervous system or, more commonly, as single neurons isolated in primary culture (pictured right).

近期论文

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Magoski NS. Electrical synapses and neuroendocrine cell function. In: Network functions and plasticity: Perspectives from studying neuronal electrical coupling in microcircuits, Elsevier. J Jing Ed, pp 139-162 (peer-reviewed). Sturgeon RM, Magoski NS. Diacylglycerol-mediated regulation of Aplysia bag cell neuron excitability requires protein kinase C. J Physiol 594:5573-5592. PDF White SH, Sturgeon RM, Magoski NS. Nicotine inhibits potassium currents in Aplysia bag cell neurons. J Neurophysiol 115:2635-2648. PDF Groten CJ, Rebane JT, Hodgson HM, Chauhan AK, Blohm G, Magoski NS. Ca2+ removal by the plasma membrane Ca2+-ATPase influences the contribution of mitochondria to activity-dependent Ca2+ dynamics in Aplysia neuroendocrine cells. J Neurophysiol 115:2615-2634. PDF Beekharry CC, Zhu GZ, Magoski NS. Role for electrical synapses in shaping the output of coupled peptidergic neurons from Lymnaea. Brain Res 1603:8-21. PDF Groten CJ, Magoski NS. PKC enhances the capacity for secretion by rapidly recruiting covert voltage-gated Ca2+ channels to the membrane. J Neurosci 35:2747-2765. PDF Dargaei Z, Standage D, Groten CJ, Blohm G, Magoski NS. Ca2+-induced uncoupling of Aplysia bag cell neurons. J Neurophysiol 113:808-821. PDF Dargaei Z, Phillip L.W. Colmers PLW, Hodgson HM, Magoski NS. Electrical coupling between Aplysia bag cell neurons: characterization and role in synchronous firing. J Neurophysiol 112:2680-2696. PDF White SH, Carter CJ, and Magoski NS. A potentially novel nicotinic receptor in Aplysia neuroendocrine cells. J Neurophysiol 112:446-462. PDF Akhmedov K, Rizzo V, Kadakkuzha BM, Carter CJ, Magoski NS, Capo TR, and Puthanveettil SV. Decreased response to acetylcholine during aging of Aplysia neuron R15. PLoS ONE 8:e84793 doi:10.1371/journal.pone.0084793. PDF Hickey CM, Groten CJ, Sham L, Carter CJ, and Magoski NS. Voltage-gated Ca2+ influx and mitochondrial Ca2+ initiate secretion from Aplysia neuroendocrine cells. Neuroscience 250:755-772. PDF Groten CJ, Rebane JT, Blohm G, and Magoski NS. Separate Ca2+ sources are buffered by distinct Ca2+ handling systems in Aplysia neuroendocrine cells. J Neurosci 33:6476-6491. PDF White SH and Magoski NS. Acetylcholine-evoked afterdischarge in Aplysia bag cell neurons. J Neurophysiol 107:2672–2685. PDF Tam AKH, Gardam KE, Lamb S, Kachoei BA, and Magoski NS. Role for protein kinase C in controlling Aplysia bag cell neuron excitability. Neuroscience 179:41-55. PDF Hickey CM, Geiger KE, Groten CJ, and Magoski NS. Mitochondrial Ca2+ activates a cation current in Aplysia bag cell neurons. J Neurophysiol 103:1543-1556. PDF Tam AKH, Geiger JE, Hung AY, Groten CJ, and Magoski NS. Persistent Ca2+ current contributes to a prolonged depolarization in Aplysia bag cell neurons. J Neurophysiol 102:3753-3765. PDF Geiger JE, Hickey CM, and Magoski NS. Ca2+ entry through a non-selective cation channel in Aplysia bag cell neurons. Neuroscience 162:1023-1038. PDF Gardam KE and Magoski NS. Regulation of cation channel voltage- and Ca2+-dependence by multiple modulators. J Neurophysiol 102:259-271. PDF Geiger JE and Magoski NS. Ca2+-induced Ca2+-release in Aplysia bag cell neurons requires interaction between mitochondrial and endoplasmic reticulum stores. J Neurophysiol 100:38-49 PDF Gardam KE, Geiger JE, Hickey CM, Hung AY and Magoski NS. Flufenamic acid affects multiple currents and causes intracellular Ca2+ release in Aplysia bag cell neurons. J Neurophysiol 100:24-37 PDF Hung AY and Magoski NS. Activity-dependent initiation of a prolonged depolarization in Aplysia bag cell neurons: role for a cation channel. J Neurophysiol 97:2465-2479 PDF Kachoei BA, Knox RJ, Uthuza D, Levy S, Kaczmarek LK, and Magoski NS. A store-operated Ca2+ influx pathway in the bag cell neurons of Aplysia. J Neurophysiol 96:2688-2698 PDF Lupinsky DA and Magoski NS. Ca2+-dependent regulation of a non-selective cation channel from Aplysia bag cell neurones. J Physiol 575:491-506 PDF Magoski NS and Kaczmarek LK. Association/Dissociation of a channel-kinase complex underlies state-dependent modulation. J Neurosci 25:8037-8047 PDF Zhang Y, Joiner WJ, Bhattacharjee A, Rassendren F, Magoski NS, and Kaczmarek LK. The appearance of a PKA-regulated splice isoform of Slo is associated with the maturation of neurons that control reproductive behavior. J Biol Chem 279:52324-52330. PDF