Kan, Frederick W.K - Queen's University - Department of Biomedical and Molecular Sciences

个人简介

The fallopian tube or oviduct occupies a unique position within the female reproductive tract where it provides the optimal environment for many critical reproductive events to take place. These include gamete transport and maturation, sperm-egg interaction, fertilization, and early embryo development. Our current research focuses on how the post-ovulatory environment in the oviduct influences gamete maturation, fertilization, and pre-implantation development. We have recently produced a human recombinant oviduct-specific glycoprotein, oviductin, and we are studying its role during the early events of fertilization. Further, we are interested in the effect of diabetes on endometrial functions at reproductive age and the use of low-dose immunosuppresant therapy in ameliorating and restoring normal reproductive functions in diabetes.

研究领域

Our current research interest is the regulatory role of glycoproteins secreted by the oviduct in sperm-egg interaction and reproductive functions. Our research focuses on the study of how the postovulatory environment in the oviduct influences gamete maturation, fertilization, and preimplantation development. We are also interested in the structure-function relationship between oviductal secretions and other ovary-specific glycoproteins that make up the zona pellucida of postovulatory oocytes. We have recently produced a human recombinant oviduct-specific glycoprotein (oviductin). We are particularly interested in studying the role of human oviductin in stimulating the increase in tyrosine phosphorylation of sperm proteins during capacitation and in the enhancement of sperm-egg binding with the hope of defining sperm quality and fertilizing competence for proper and ethical use of assisted reproductive technology (ART) in alleviating male infertility. Another project being undertaken in our laboratory involves the study of MUC1 in endometrial functions. MUC1 is a member of the mucin family of molecules which is expressed at the apical surface of the glandular and luminal epithelium of the endometrium. To gain a better understanding of the effects of diabetes on endometrial functions at reproductive age, we utilize the spontaneously developing type 1 diabetic NOD mouse and type II diabetic mouse models to investigate the effects of diabetes on the endometrial expression and production of mucins and their regulatory cytokines during the estrous cycle and embryo implantation. Sources of Research Funds: Natural Sciences and Engineering Research Council of Canada, Kingston General Hospital Medical Research Fund

近期论文

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*McBride, D.S., C. Boisvert, G. Bleau, F.W.K. Kan (2004) Detection of nascent and/or mature forms of oviductin in the female reproductive tract and post-ovulatory oocytes by use of a polyclonal antibody against recombinant hamster oviductin. Journal of Histochemistry & Cytochemistry 52:1001-1009 *McBride, D.S., I. Brockhausen, F.W.K. Kan (2005) Detection of glycosyltransferases in the Golden Hamster (Mesocricetus auratus) oviductin and evidence for the regulation of O-glycan biosynthesis during the estrous cycle. Biochimica et Biophysica Acta 1721: 107-115 *Ling, L., Y.L. Lee, K.F. Lee, S.W. Tsao, W.S.B. Yeung, F.W.K. Kan (2005) Human oviductin mRNA is expressed in an immortalized human oviductal cell line. Sterility and Fertility 84: 1095-1103 Kan, F.W.K., *D.S. McBride (2005) Estrous Cycle-dependent glycosylation and post-translational modification of oviductin in the golden hamster (Mesocricetus auratus). In: New Impact in protein modifications in the regulation of reproduction.Toshinobu Tokumoto, Editor, Chapter 8, Research Signpost, Kerala, India, p.135-153 Kan, F.W.K. and *P.W.B. Esperanzate (2006) Surface mapping of binding of oviductin to the plasma membrane of golden hamster spermatozoa during in vivo capacitation and acrosome reaction. Molecular Reproduction and Development 73: 756-766 Kan, F.W.K. (2006) Molecular dissection of the mouse zona pellucida: An electron microscopic perspective utilizing colloidal-gold labeling methods. Methods in Molecular Biology, Vol.347: Glycobiology Protocols, Edited by Inka Brockhausen, Chapter 19, Humana Press Inc., Totowa, New Jersey, p.289-304 Kan, F.W.K., P.-K. Forkert, M. Wade (2007) Trichloroethylene exposure elicits damage in epididymal epithelium and spermatozoa in mice. Histology and Histopathology 22: 977-988 Moos, M.P.W., J.D. Mewbiurn, F.W.K. Kan, S. Ishii, M. Abe, K. Sakimura, K. Noguchi, T. Shimizu, C.D. Funk. (2008) Cysteinyl leukotriene 2 receptor mediated vascular permeability via transendothelial vesicle transport. FASEB Journal 172: 592-602 Yan, J., J. Suzuki, X.-M. Yu, F.W.K. Kan, J. Qiao, R.C. Chian. (2010) Cryo-survival, fertilization and early embryonic development of vitrified oocytes derived from mice of different reproductive age. Journal of Assisted Reproduction and Genetics 27:605-611. Yan, J., J. Suzuki, X.-M. Yu, J. Qiao, F.W.K. Kan, R.C. Chian. (2011) Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification. Journal of Assisted Reproduction and Genetics 28:643-649 Wu, Z., F.W.K. Kan, Y.-M. She, V.K. Walker (2012) Biofilm, ice recrystallization inhibition and freeze-thaw protection in an epipihyte community. Applied Biochemistry and Microbiology 48:403-410 *Albaghdadi A. and F.W.K. Kan. (2012) Endometrial receptivity defects and impaired implantation in diabetic NOD mice. Biology of Reproduction Aug 2; 87(2):30. doi: 10.1095/bioreprod.112.100016. Print2012 Aug. *Yang X., S. Tao, R. Orlando, I. Brockhausen, F.W.K. Kan. (2012) Structures and biosynthesis of the N- and O-glycans of recombinant human oviduct-specific glycoprotein expressed in human embryonic kidney cells. Carbohydrates Research 358:47-55 *Saccary L., Y.M. She, R. Oko, F.W.K. Kan (2013) Hamster oviductin regulates tyrosine phosphorylation of sperm proteins during in vitro capacitation. Biology of Reproduction Aug 22; 89(2):38. doi: 10.1095/biolreprod.113.109314. Print 2013 Aug. *Khosravi F., R.L. Reid, A. Moini, F. Abolhassani, F.W.K. Kan (2013) In vitro development of human primary follicles to preantral stage after vitrification. J. Assist. Reprod. Genet. 30:1397-1406 Kan, F.W.K. (2014) Freeze-fracture immunocytochemistry: fracture-label and label-frafture for the localization of membrane proteins. Curr Protoc Cell Biol. Dec 1; 65:4.28.1-2.28.15. doi:10.1002/0471143030.cb0428s65 Yang X, Y. Zhao, X Yang, F.W.K. Kan (2015) Recombinant hamster oviductin is biologically active and exterts positive effects on sperm functions and sperm-oocyte binding. PLos One. April 7; 10(4):e0123003. doi: 10.1371/journal.pone.0123003. eCollection 2015. (*) indicates graduate student and/or research trainee in my laboratory