王炜 - 复旦大学 - 生命科学学院

个人简介

王炜，博士生导师，复旦大学生命科学学院青年研究员。2008年毕业于山东大学医学院，获得临床医学学士学位，2014年在美国密苏里大学（University of Missouri, Columbia）获得生物化学博士学位，2015至2017年在北京大学化学院化学生物学系进行博士后研究。2018-2022年任职于上海交通大学医学院分子医学研究院，2023年2月入职复旦大学生科院微生物学与免疫学系。研究聚焦于通过发展化学的探针工具与研究策略，探究与人类健康密切相关的各类复杂细菌体系的微生物活动与功能。近年来针对肠道菌群发展了一系列定性、定量研究的化学生物学新方法，发表包括Nature Communications、Science Advances、Accounts of Chemical Research、Angew. Chem. Int. Ed.在内的多篇研究论文，并于2021年获得国家自然科学基金优秀青年基金资助。

研究领域

在我们肠道中时刻都进行着大量有微生物参与的化学反应和生物作用过程，这些复杂的反应和过程对人类健康存在着深刻影响。例如大量的各类被吸收入血的微生物代谢产物、与免疫系统存在复杂相互作用的抗原表位、广泛影响神经系统功能的多类化合物等等。阐明这些反应和过程将会有助于更好地理解我们与这些“亲密邻居”之间的合作和对抗关系。我们通过发展适用于活体菌群研究的分子探针工具和化学研究策略，希望能够在原位探究细菌参与的这些化学反应和生物过程。通过将这些工具与新的成像方法、生物信息学方法，以及多组学分析相结合，开拓“化学微生物组学”这一新的菌群研究思路，进一步加深我们对肠道中这些微生物“暗物质”的认识。近阶段主要的研究方向如下：（1）发展肠道菌群成像新方法（2）开发用于肠道细菌在体微生物活动研究的分子探针（3）临床微生物研究、诊断与治疗的新策略

近期论文

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Dongxia Chen#, Junnan Guo#, Ao Li, Chengjie Sun, Huibin Lin, Hongyu Lin*, Chaoyong Yang*, Wei Wang*, JinhaoGao*. "Metabolic fluorine labeling and hotspot imaging of dynamic gut microbiota in mice." Science Advances, 2023. doi: 10.1126/sciadv.abg6808 Ningning Xu#, Liyuan Lin#, Yahui Du, Huibin Lin, Jia Song, Chaoyong Yang*, Wei Wang*. "Visualizing germination of microbiota endospores in the mammalian gut." Gut Microbes, 2022. doi: 10.1080/19490976.2022.2125737 Juan Gao#, Di Sun#, Bei Li, Chaoyong Yang*, Wei Wang*. "Integrated identification of growth pattern and taxon of bacterium in gut microbiota via confocal fluorescence imaging-oriented single-cell sequencing" mLife, 2022. doi: 10.1002/mlf2.12041 Juan Gao#, Juanxiu Qin#, Chenling Ding#, Yuan Gao, Junnan Guo, Min Li*, Chaoyong Yang*, Wei Wang*. "Fluorescent metabolic labeling-based quick antibiotic susceptibility test for anaerobic bacteria." RSC Chemical Biology, 2022. doi: 10.1039/D2CB00163B Huibin Lin, Chaoyong Yang*, Wei Wang*. "Imitate to illuminate: labeling of bacterial peptidoglycan with fluorescent and bio-orthogonal stem peptide-mimicking probes." RSC Chemical Biology, 2022. doi: 10.1039/D2CB00086E Juan Gao#, Junnan Guo#, Jianxiao Chen#, Chenling Ding, Jiemin Wang, Qian Huang, Ying Jian, Xianyuan Zhao, Min Li, Yuan Gao, Chaoyong Yang*, Wei Wang*. "D-Amino acid-based metabolic labeling enables fast antibiotic susceptibility test of both isolated bacteria and bronchoalveolar lavage fluid." Advanced Healthcare Materials, 2021. doi: 10.1002/adhm.202101736 Liyuan Lin#, Jia Song#, Jian Li#, Xiaolei Zuo, Hong Wei*, Chaoyong Yang*, Wei Wang*. "Imaging the in vivo growth patterns of bacteria in human gut microbiota." Gut Microbes, 2021. doi: 10.1080/19490976.2021.1960134 Huibin Lin, Liyuan Lin, Yahui Du, Juan Gao, Chaoyong Yang, Wei Wang*. "Biodistributions of L,D-transpeptidases in gut microbiota revealed by in vivo labeling with peptidoglycan analogs."ACS Chemical Biology, 2021. doi: 10.1021/acschembio.1c00346 Ru Chen#, Jia Song#, Liyuan Lin, Jie Liu*, Chaoyong Yang*, Wei Wang*. "Visualizing the growth and division of rat gut bacteria by D-amino acid-based in vivo labeling and FISH staining." Frontiers in Molecular Biosciences, 2021. doi: 10.3389/fmolb.2021.681938 Liyuan Lin, Yahui Du, Jia Song, Wei Wang*, Chaoyong Yang*. "Imaging commensal microbiota and pathogenic bacteria in the gut." Accounts of Chemical Research, 2021. doi: 10.1021/acs.accounts.1c00068 Wei Wang#,*, Ni Zhang#, Yahui Du#, Juan Gao, Min Li, Liyuan Lin, Daniel M. Czajkowsky, Xiaowei Li*, Chaoyong Yang*, Zhifeng Shao. "Three-dimensional quantitative imaging of native microbiota distribution in the gut." Angew. Chem. Int. Ed., 2021. doi: 10.1002/anie.202010921 Liyuan Lin, Qiuyue Wu, Jia Song, Yahui Du, Juan Gao, Yanling Song, Wei Wang*, Chaoyong Yang*. "Revealing the in vivo growth and division patterns of mouse gut bacteria." Science Advances, 2020. doi: 10.1126/sciadv.abb2531 Liyuan Lin, Jia Song, Yahui Du, Qiuyue Wu, Juan Gao, Yanling Song, Chaoyong Yang, Wei Wang*. "Quantification of bacterial metabolic activities in the gut by D-amino acid-based in vivo labeling." Angew. Chem. Int. Ed., 2020. doi: 10.1002/anie.202004703 Wei Wang#, Qinglai Yang#, Yahui Du#, Xiaobo Zhou, Xiaochen Du, Qiuyue Wu, Liyuan Lin, Yanling Song, Fuyou Li, Chaoyong Yang*, Weihong Tan*. "Metabolic labeling of peptidoglycan with NIR-II dye enables in vivo imaging of gut microbiota." Angew. Chem. Int. Ed., 2020. doi: 10.1002/anie.201910555 Wei Wang*, Yahui Du, Shuai Yang, Xiaochen Du, Min Li, Bingqian Lin, Jie Zhou, Liyuan Lin, Yanling Song, Juan Li, Xiaolei Zuo*, Chaoyong Yang*. "Bacterial extracellular electron transfer occurs in mammalian gut." Analytical Chemistry, 2019. doi: 10.1021/acs.analchem.9b03176 Wei Wang, Liyuan Lin, Yahui Du, Yanling Song, Xiaoman Peng, Xing Chen*, Chaoyong Yang*. " Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes." Nature Communications, 2019. doi: 10.1038/s41467-019-09267-x Wei Wang*, Xing Chen*. "Antibiotics-based fluorescent probes for selective labeling of Gram-negative and Gram-positive bacteria in living microbiotas." Science China Chemistry, 2018. doi:10.1007/s11426-018-9236-5 Wei Wang, Yuntao Zhu, Xing Chen*. "Selective imaging of Gram-negative and Gram-positive microbiotas in the mouse gut." Biochemistry, 2017. doi: 10.1021/acs.biochem.7b00539 Wei Wang, Deborah L Chance, Valeri V Mossine, Thomas P Mawhinney*. "RAFT-based tri-component fluorescent glycopolymers: synthesis, characterization and application in lectin-mediated bacterial binding study." Glycoconjugate Journal, 2014. doi: 10.1007/s10719-013-9508-4