Pandhal, Jagroop - University of Sheffield - Department of Chemical and Biological Engineering

个人简介

BSc (Hons), MSc (Hons), PhD I have primarily worked with environmentally sourced samples from a microbiological and molecular biology perspective. After gaining experience in molecular ecology through employment at Sheffield Molecular Genetics Facility I aimed to combine my interests in environmental research with potential applications to issues relevant to societies challenges, and undertook a Masters in Research in Applied Biosciences. During my doctoral studies I worked at the interface of life sciences and engineering developing quantitative proteomics tools to complement environment-focused projects with functional characterisation. I became interested in proteomics as proteins are the functional entities in cells, driving biological processes. Therefore the proteome provides a window into environmental adaptation mechanisms. The discovery of a potentially novel bacterial clade with a unique combination of tolerance strategies led to the work being featured in an editorial special of Proteomics journal with a podcast interview and I received the Thring Prize for best thesis 2008. As a Post Doctoral Research Associate, the BBSRC Bioprocess Research Industry Club funded me where I used proteomics as a tool in conjunction with inverse and traditional metabolic engineering techniques, to produce human therapeutic proteins. More specifically this involved characterisation and quantitation of protein post-translational modifications in bacterial cells. Although the aim of this research is bioprocessing, the key element is the development of -omics technologies, building on previous expertise with added complexity of glycosylating components. During this time I lectured in biotechnology, bioprocessing and bioengineering. New research perspectives are in the field of metaproteomics, the study of proteins in complex environmental samples. Natural biological systems comprise complex combinations of organisms. It is commonly predicted that far less than 1% of microorganisms have been cultured in the laboratory, leaving a wealth of biological knowledge and biotechnological potential untouched and potential distortion of our understanding of microbial functions and adaptations. Moreover, microorganisms commonly function in communities where they interact with each other through exchange of metabolites, genes and cell-cell interactions. Therefore metaproteomics can provide a signature of ecosystem function. This information can be combined with traditional ecology data (theory and experiments), which is the aim of my present research as a NERC fellow.

研究领域

My Research interests are Environmental proteomics Metaproteomics Metabolic Engineering Ecological Engineering (synthetic ecology) Biomanufacturing Ecosystems Services (industrial ecology) It is widely recognised that the fundamental training of a biologist and an engineer is different. Mathematical theories and quantitative methods are at the forefront of engineering approaches, and therefore their application to complex systems, including biological, is a useful attribute. However, biologists have the advantage of formulating better testable hypotheses, experimental designs and data interpretation from these complex biological systems. This is namely due to different techniques and strategies used by life scientists to qualitatively decipher complex systems. The skills of an engineer and life scientist are therefore complementary. I work at this interface to reveal information about complex biological systems.

近期论文

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Roccuzzo S, Beckerman AP & Pandhal J (2016) The use of natural infochemicals for sustainable and efficient harvesting of the microalgae Scenedesmus spp. for biotechnology: insights from a meta-analysis.. Biotechnol Lett. View this article in White Rose Research Online Russo D, Beckerman AP, Couto N & Pandhal J (2016) A metaproteomic analysis of the response of a freshwater microbial community under nutrient enrichment.. Frontiers in Microbiology. View this article in White Rose Research Online Chiverton LM, Evans C, Pandhal J, Landels AR, Rees BJ, Levison PR, Wright PC & Smales CM (2016) Quantitative definition and monitoring of the host cell protein proteome using iTRAQ - a study of an industrial mAb producing CHO-S cell line. Biotechnology Journal. View this article in White Rose Research Online Jaffé SRP, Strutton B, Pandhal J & Wright PC (2015) Inverse metabolic engineering for enhanced glycoprotein production in Escherichia coli. Methods in Molecular Biology, 1321, 17-35. Russo D, Pandhal J & Beckerman A (2015) MULTIDISCIPLINARY ANALYSIS OF A FRESHWATER LAKE MICROBIAL COMMUNITY UNDER DIFFERING NUTRIENT REGIMES. EUROPEAN JOURNAL OF PHYCOLOGY, 50, 117-117. Pandhal J & Noirel J (2014) Synthetic microbial ecosystems for biotechnology.. Biotechnol Lett, 36(6), 1141-1151. Phansopa C, Roy S, Rafferty JB, Douglas CW, Pandhal J, Wright PC, Kelly DJ & Stafford GP (2014) Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species.. Biochem J, 458(3), 499-511. View this article in White Rose Research Online