Seward, Elizabeth - University of Sheffield - Department of Biomedical Science

个人简介

2003- Present: Senior Lecturer, Department of Biomedical Science, University of Sheffield, UK 2000-2003: Lecturer, Department of Cell Physiology & Pharmacology, University of Leicester, UK 1996-2000: Lecturer, Department of Pharmacology, University of Bristol, UK. 1994-1995: Postdoctoral Fellow, Glaxo Institute for Molecular Biology, Geneva, Switzerland. 1991-1994: Medical Research Council of Canada Postdoctoral Fellow, Dept Anatomy & Neurobiology, Medical College of Pennsylvania, Philadelphia, USA. 1991: Postdoctoral Researcher, Dept. Pharm., University of California, San Diego, USA 1986-1990: Ph.D. Department of Pharmacolgy, University of Cambridge, UK. Member of Trinity College. 1985: M. Phil. Department of Pharmacology, University of Cambridge, UK. Member of Trinity College. 1981-1984: B.Sc. (First Class Honours). Department of Biochemistry, McGill University, Montreal, Canada

研究领域

Aberrant secretion of neurotransmitters, hormones or immune mediators contributes to the pathology of a wide variety of chronic neurological, endocrine and inflammatory diseases ranging from stress and hypertension through to asthma and irritable bowel syndrome. Research in our lab is focussed on identifying the signalling pathways and molecules controlling secretion from neurones and mast cells, with a special interest in voltage-gated (CaV), ligand-gated (P2X and nAChR), receptor-operated (TRPC) and store-operated (Orai) calcium channels, IgE and G protein coupled receptors (P2Y, Histamine), and SNARE regulatory proteins (synaptotagmins, Doc2 and Munc13). Most of our work is performed at the level of isolated primary cells using high resolution techniques including patch-clamp electrophysiology, carbon fibre amperometry, calcium imaging and total internal reflection fluorescence microscopy with various fluorescence-based biosensors. Recent highlights of our research include (1) the discovery of ATP-sensitive P2X receptors on human lung mast cells, activation of which may contribute to the pathophysiology of asthma, (2) the first demonstration of Munc13 as an essential effector of phospholipase C-coupled G protein coupled receptor regulation of neurotransmitter release in mammalian cells, and (3) the modulatory action of synaptotagmin IV on the calcium-sensitivity of the neuronal fusion machinery. My research group is part of the Centre for Sensory Neuroscience and the Centre for Membrane Interactions and Dynamics (CMIAD)

近期论文

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Wareham KJ & Seward EP (2016) P2X7 receptors induce degranulation in human mast cells. Purinergic Signalling, 12(2), 235-246. View this article in White Rose Research Online Farrington J, Seward EP & Peachell PT (2014) Mast cell purification protocols. Methods Mol Biol, 1192, 49-55. Kay LJ, Gilbert M, Pullen N, Skerratt S, Farrington J, Seward EP & Peachell PT (2013) Characterization of the EP receptor subtype that mediates the inhibitory effects of prostaglandin E2 on IgE-dependent secretion from human lung mast cells. Clinical and Experimental Allergy, 43, 741-751. Farrington J, Tree-Booker CL, Peachell P & Seward EP (2012) Biophysical and Pharmacological Characterisation of Calcium Channels in Human Mast Cells. BIOPHYSICAL JOURNAL, 102(3), 425A-425A.