Brown, Stephen - University of Sheffield - Department of Biomedical Science

个人简介

2008-present: University of Sheffield (Biomedical Sciences), Sheffield RNAi Screening Facility Manager (SRSF) 2004-2008: University of Manchester (Faculty of Life Sciences), MRC Career Development Fellowship 2004: University of Cambridge (Pathology), Post-Doctoral research 1997- 2003: University of Cambridge (Zoology), Post-Doctoral research 1997: University of Nottingham, PhD Research Interests

研究领域

Functional Genomics and RNAi Screening Many diseases have an unknown or poorly defined molecular diagnosis. There is a need to identify the molecular nature of these orphan diseases and the changes these mutations cause to cells and tissues. The Sheffield RNAi Screening Facility uses robotics and automated systems to leverage RNAi/small molecules and genome editing tools to identify novel biological and disease candidates. I use Drosophila and Human cell models of disease. In particular, I use normal, cancer and Human embryonic stem cells. Recently we have been growing 3D tumour spheroids for screening. I collaborate with Dr. Xiubo Zhao using self-assembling nano-peptides, Dr. Ivana Barbaric and Dr. Paul Gokhale investigating miR regulation of Human embryonic stem cells and Dr. Martin Zeidler identifying new members of the JAK/STAT signalling pathway. I have managed over 50 RNAi library screens for both UK and international scientists. My laboratory uses both automation (robotics) and molecular cell biology to identify genes involved in disease. I am also involved in teaching Masters and PhD students in the art of “Functional Genomics”.

近期论文

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Balasubramanian M, Hurst J, Brown S, Bishop NJ, Arundel P, DeVile C, Pollitt RC, Crooks L, Longman D, Caceres JF, Shackley F, Connolly S, Payne JH, Offiah AC, Hughes D., DDD Study, Parker MJ, Hide W, Skerry TM. (2017) Compound heterozygous variants in NBAS as a cause of atypical osteogenesis imperfect. Bone 94: 65–74 Fisher KH, Stec W, Brown S, Zeidler MP. (2016) Mechanisms of JAK/STAT pathway negative regulation by the short coreceptor Eye Transformer / Latran. Mol Biol Cell. Feb 1;27(3):434-41. Thomas S, Fisher KH, Snowden JA, Danson SJ, Brown S, Zeidler MP. (2015) “Methotrexate Is a JAK/STAT Pathway Inhibitor.” PLoS One. Jul 1;10(7):e0130078. Thomas S, Fisher KH, Snowden J, Danson S, Brown S, Zeidler M. (2015) “Effect of methotrexate on JAK/STAT pathway activation in myeloproliferative neoplasms.” Lancet. Feb 26;385