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个人简介

教育经历 2008-08至2011-07,加州大学洛杉矶分校,博士后,导师:James C. Liao教授(美国科学院院士、美国工程院院士、美国国家发明家科学院院士,第三世界科学院院士、台湾中央研究院院士、现任台湾中央研究院院长) 2006-05至2008-08,加州大学洛杉矶分校,博士后,导师:Jay D. Gralla教授 2002-09至2005-12,巴黎第七大学,分子微生物学及病毒学,博士(联合培养、分别获得中法两国博士学位),导师:Annie Kolb研究员 2000-09至2005-12,北京大学,生物化学及分子生物学,博士,导师:王忆平教授 1995-09至1999-06,南开大学,微生物学,学士 科研与学术工作经历 2021-01至今,北京理工大学,生命学院,长聘教授 2019-10至今,北京理工大学,生命学院,副院长(分管研究生、学科) 2019-09至今,北京理工大学,生命学院,生物技术专业责任教授 2019-01至今,北京理工大学,工业和信息化部“分子医学与生物诊疗”重点实验室,副主任 2017-09至2020-12,北京理工大学,生命学院,生物工程专业责任教授 2015-09至2020-12,北京理工大学,生命学院,教授 2014-11至2015-09,加州大学洛杉矶分校苏州先进技术研究院,生物能源研究中心,实验室主任(兼) 2011-08至2015-09,Nantworks集团Easel生物技术公司,项目主管、资深研究员

研究领域

碳中和 二氧化碳的资源化,以固碳微生物实现二氧化碳的高值转化 基因编辑 原核、真核细胞的基因编辑、高鲁棒性的底盘宿主构建;方法学开发 代谢工程 大宗化学品、药物中间体的微生物合成途径构建及高效生产 酶工程 塑料降解酶;生物传感器的研发 生物信息学 植物功能基因挖掘;微生物进化机制

近期论文

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Hao Guo*, Xiaoyan Ma*, Ning Wang, Tingting Ding, Bo Zheng, Liwei Guo, Chaoyong Huang, Wuyuan Zhang, Lichao Sun, Yi-Xin Huo*. A transfer ribonucleic acid Modification-based Identification of amiNo acid Overproducers (AMINO). 2023 (submitted) Hao Guo, Tongtong Chen, Hanrong Zhu, Huiyan Wang, Yi-Xin Huo*. Engineering key amino acid residues of typical pentacyclic triterpene synthases for improving the activity. 2023 (submitted) Junkai Tuo, Said Nawab, Xiaoyan Ma*, Yi-Xin Huo. Recent advances in screening amino acid overproducers. Engineering Microbiology, 2022: https://doi.org/10.1016/j.engmic. 2022.100066 (发表年月 2023-01) Changgeng Xu, Qin Zou, Jiheng Tian, Mengyuan Li, Baowen Xing, Julia Gong, Jiangyun Wang*, Yi-Xin Huo*, Shuyuan Guo*. Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids. ACS Synth Biol. 2023 Feb 17;12(2):583-595. (影响因子5.249,发表年月2023-02) Yuhui Du#, Li Li#, Yue Zheng, Jiaheng Liu, Julia Gong, Zekai Qiu, Yanni Li, Jianjun Qiao, Yi-Xin Huo. Incorporation of Non-Canonical Amino Acids into Antimicrobial Peptides: Advances, Challenges, and Perspectives. Appl Environ Microbiol. 2022 Dec 13;88(23):e0161722. (影响因子5.005,发表年月2022-12) Bo Zheng, Shengzhu Yu, Zhenya Chen* and Yi-Xin Huo*. A consolidated review of commercial-scale high-value products from lignocellulosic biomass. Front Microbiol. 2022 Aug 23;13:933882. (影响因子5.64,发表年月2022-08) Hao Guo, Huiyang Wang, Tongtong Chen, Liwei Guo, Lars M Blank, Birgitta E Ebert, Yi-Xin Huo*. Engineering Critical Amino Acid Residues of Lanosterol Synthase to Improve the Production of Triterpenoids in Saccharomyces cerevisiae. ACS Synth Biol. 2022 Aug 19;11(8):2685-2696. (影响因子5.249,发表年月2022-08) Liwei Guo, Lichao Sun* and Yi?Xin Huo. Toward bioproduction of oxo chemicals from C1 feedstocks using isobutyraldehyde as an example. Biotechnol Biofuels Bioprod. 2022 Aug 9;15(1):80. (影响因子7.67,发表年月2022-08) Ning Wang, Yi-Xin Huo*. Using genome and transcriptome analysis to elucidate biosynthetic pathways. Curr Opin Biotechnol. 2022, 75: 102708.(影响因子9.74,发表年月2022-06) Tong Wu, Yumei Liu, Jinsheng Liu, Zhenya Chen*, Yi-Xin Huo*. Metabolic engineering and regulation of diol biosynthesis from renewable biomass in Escherichia coli. Biomolecules. 2022, 12:715.(影响因子6.064,发表年月2022-06) 刘玉美,毋彤,陈振娅,霍毅欣*。非天然氨基酸及非天然蛋白合成的研究进展。生物加工过程。2022,20(02):182-194. (影响因子1.109,发表年月2022-06) Zhenya Chen, Tongtong Chen, Shengzhu Yu, Yi-Xin Huo*. A high-throughput visual screening method for p-hydroxybenzoate hydroxylase to increase phenolic compounds biosynthesis. Biotechnol Biofuels Bioprod . 2022, 15(1): 1-15.(影响因子7.67,发表年月2022-05) Mengyuan Li, Yi-Xin Huo*, Shuyuan Guo*. CRISPR-Mediated Base Editing: From Precise Point Mutation to Genome-Wide Engineering in Nonmodel Microbes. Biology. 2022, 11(4): 571.(影响因子5.007,发表年月2022-04) Jiheng Tian, Baowen Xing, Mengyuan Li, Changgeng Xu, Yi-Xin Huo*, Shuyuan Guo*. Efficient Large-Scale and Scarless Genome Engineering Enables the Construction and Screening of Bacillus subtilis Biofuel Overproducers. Int. J. Mol. Sci. 2022, 23, 4853.(影响因子5.923,发表年月2022-04) Yuhui Du, Wei Song, Zhiqiu Yin, Shengbo Wu, Jiaheng Liu, Ning Wang, Hua Jin, Jianjun Qiao, Yi-Xin Huo*. Genomic Analysis Based on Chromosome-Level Genome Assembly Reveals an Expansion of Terpene Biosynthesis of Azadirachta indica. Front Plant Sci. 2022, 13: 853861.(影响因子5.753,发表年月2022-04) Bo Zheng, Yinchao He, Pengxiang Zhang, Yi-Xin Huo*, Yanbin Yin. Polyphenol Utilization Proteins in the Human Gut Microbiome. Appl Environ Microbiol. 2022 Feb 8;88(3):e0185121.(影响因子5.005,发表年月2022-02) Tong Wu#, Zhenya Chen#, Shuyuan Guo, Cuiying Zhang, Yi-Xin Huo*. Engineering transcription factor BmoR mutants for constructing multifunctional alcohol biosensors. ACS Synth Biol. 2022, 11(3): 1251-1260(影响因子5.249,发表年月2022-02) Shengzhu Yu, Bo Zheng, Zhenya Chen*, Yi-Xin Huo. Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids. Microb Cell Fact. 2021. 20(1): 1-14(影响因子6.352,发表年月2021-12) Luyao Zhao, Zhenya Chen, Sheng Lin, Tong Wu, Shengzhu Yu, Yi-Xin Huo*. In Vitro Biosynthesis of Isobutyraldehyde Through the Establishment of a One-Step Self-Assembly-Based Immobilization Strategy. J Agr Food Chem. 2021. 69(48): 14609-19(影响因子5.279,发表年月2021-11) Huiyan Wang#, Hao Guo#, Ning Wang*, Yi-Xin Huo*. Toward the Heterologous Biosynthesis of Plant Natural Products: Gene Discovery and Characterization. ACS Synth Biol. 2021(影响因子5.249,发表年月2021-11) Xiaoyan Ma#, Lianjie Ma#, Yi-Xin Huo*. Reconstructing the transcription regulatory network to optimize resource allocation for robust biosynthesis. Trends Biotechnol. 2021(影响因子19.526,发表年月2021-11) 马晓焉,王雪芹,马炼杰,霍毅欣*。高级醇的微生物绿色制造。生物工程学报。2021. 37(05): 1721-36(影响因子0.977,发表年月2021-05) Jingge Wang#, Chaoyong Huang#, Kai Guo, Lianjie Ma, Xiangyu Meng, Ning Wang*, Yi-Xin Huo*. Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA. Syn Syst Biotechno. 2020. 5(4): 333-42(影响因子4.781,发表年月2020-12) Huiyan Wang, Ning Wang*, Yi-Xin Huo*. Multi-tissue transcriptome analysis using hybrid-sequencing reveals potential genes and biological pathways associated with azadirachtin A biosynthesis in neem (azadirachta indica). BMC Genomics. 2020. 21(1): 1-17(影响因子3.969,发表年月2020-10) Mengli Xia, Lin Hu, Yi-Xin Huo, Yu Yang*. Myroides albus sp. nov., isolated from the gut of plastic-eating larvae of the coleopteran insect Zophobas atratus. Int J Syst Evol Microbiol. 2020. 70(10): 5460-6(影响因子2.747,发表年月2020-09) Ziyu Xu, Mengli Xia, Yi-Xin Huo, Yu Yang*. Intestinirhabdus alba gen. nov., sp. nov., a novel genus of the family Enterobacteriaceae, isolated from the gut of plastic-eating larvae of the Coleoptera insect Zophobas atratus. Int J Syst Evol Microbiol. 2020. 70(9): 4951-9(影响因子2.747,发表年月2020-09) Chaoyong Huang#, Liwei Guo#, Jingge Wang, Ning Wang, Yi-Xin Huo*. Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering. Appl Microbiol Biotechnol. 2020. 104(18): 7943-56(影响因子4.813,发表年月2020-08) Hao Guo, Huiyan Wang, Yi-Xin Huo*. Engineering critical enzymes and pathways for improved triterpenoid biosynthesis in yeast. ACS Synth Biol. 2020. 9(9): 2214-27(影响因子5.249,发表年月2020-08) 梁昕鑫,唐丹,霍毅欣*。蛋白源生物质的绿色生物转化。生物技术通报。2020. 36(12): 216(影响因子0.986,发表年月2020-07) Shengzhu Yu, Liwei Guo, Luyao Zhao, Zhenya Chen*, Yi-Xin Huo*. Metabolic engineering of E. coli for producing phloroglucinol from acetate. Appl Microbiol Biotechnol. 2020. 104(18): 7787-99(影响因子4.813,发表年月2020-07) Xiaoyan Ma, Lianjie Ma, Yi-Xin Huo*, Harnessing the hierarchy of transcriptional regulation: engineering of the gene expression network for efficient production, in Systems and Synthetic Metabolic Engineering. 2020, Elsevier. p. 107-24. (发表年月2020-07) Zhiqiu Yin, Jiaheng Liu, Binghai Du, Hai-Hua Ruan, Yi-Xin Huo*, Yuhui Du*, Jianjun Qiao*. Whole-Genome-Based survey for polyphyletic serovars of Salmonella enterica subsp. enterica provides new insights into public health surveillance. Int J Mol Sci. 2020. 21(15): 5226(影响因子5.924,发表年月2020-07) Jiakang Ru, Yi-Xin Huo, Yu Yang*. Microbial degradation and valorization of plastic wastes. Front Microbiol. 2020. 11: 442(影响因子5.64,发表年月2020-04) Said Nawab, Ning Wang*, Xiaoyan Ma*, Yi-Xin Huo. Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review. Microb Cell Fact. 2020. 19(1): 1-16(影响因子6.352,发表年月2020-03) Tingting Ding, Chaoyong Huang, Zeyu Liang, Xiaoyan Ma*, Ning Wang*, Yi-Xin Huo. Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact. 2020. 19(1): 1-13(影响因子6.352,发表年月2020-03) Mengli Xia, Jialei Wang, Yi-Xin Huo, Yu Yang*. Mixta tenebrionis sp. nov., isolated from the gut of the plastic-eating mealworm Tenebrio molitor L. Int J Syst Evol Microbiol. 2020. 70(2): 790-6(影响因子2.747,发表年月2020-02) Lianjie Ma#, Liwei Guo#, Yunpeng Yang, Kai Guo, Yajun Yan, Xiaoyan Ma*, Yi-Xin Huo. Protein-based biorefining driven by nitrogen-responsive transcriptional machinery. Biotechnol Biofuels Bioprod. 2020. 13(1): 1-14(影响因子7.67,发表年月2020-02) Huan Yu#, Zhenya Chen#, Ning Wang, Shengzhu Yu, Yajun Yan, Yi-Xin Huo*. Engineering transcription factor BmoR for screening butanol overproducers. Metab Eng. 2019. 56: 28-38(影响因子9.783,发表年月2019-12) Chaoyong Huang, Tingting Ding, Jingge Wang, Xueqin Wang, Liwei Guo, Jialei Wang, Lin Zhu, Changhao Bi, Xueli Zhang, Xiaoyan Ma, Yi-Xin Huo*. CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli. Appl Microbiol Biotechnol. 2019. 103(20): 8497-509(影响因子4.813,发表年月2019-10) Liyuan Jin#, Said Nawab#, Mengli Xia, Xiaoyan Ma*, Yi-Xin Huo*. Context‐dependency of synthetic minimal promoters in driving gene expression: a case study. Microb Biotechnol. 2019. 12(6): 1476-86(影响因子5.813,发表年月2019-10) 杨云彭,马晓焉,霍毅欣*。密码子优化策略在异源蛋白表达中的应用。生物工程学报。2019. 35(12): 2227-37(影响因子0.977,发表年月2019-09) 郑博,王宁*,霍毅欣。基于转录和翻译调控的氨基酸高产菌株筛选及构建策略。生物技术通报。2020. 36(4): 34(影响因子0.986,发表年月2019-09) Xiaoyan Ma#, Xinxin Liang#, Yi-Xin Huo*. Developing a transformation-independent and unbiased qPCR assay to rapidly evaluate the determinants of DNA assembly efficiency. Engineering. 2019. 5(4): 803-10(影响因子7.553,发表年月2019-08) Zhenya Chen#, Luyao Zhao#, Jiakang Ru, Shengzhu Yu, Huan Yu, Huiyong Ren, Yuhong Zhang, Wei Zhang, Fankai Lin, Yi-Xin Huo*. A novel protein purification strategy mediated by the combination of CipA and Ssp DnaB intein. J Biotechnol. 2019. 301: 97-104(影响因子2.563,发表年月2019-08) Yi-Xin Huo, Bo Zheng, Ning Wang, Yunpeng Yang, Xinxin Liang, Xiaoyan Ma*. Identifying amino acid overproducers using rare-codon-rich markers. J Vis Exp. 2019. 148: e59331(影响因子1.355,发表年月2019-06) Jiao Zhang, Fayu Yang, Yunpeng Yang, Yu Jiang, Yi-Xin Huo*. Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum. Microb Cell Fact. 2019. 18(1): 1-13(影响因子6.352,发表年月2019-03) Huan Yu, Ning Wang, Wenbo Huo, Yuhong Zhang, Wei Zhang, Yu Yang, Zhenya Chen*, Yi-Xin Huo*. Establishment of BmoR-based biosensor to screen isobutanol overproducer. Microb Cell Fact. 2019. 18(1): 1-11(影响因子6.352,发表年月2019-02) Yuan Yu, Pengcheng Chang, Huan Yu, Huiyong Ren, Danning Hong, Zeyan Li, Ying Wang, Hao Song, Yi-Xin Huo, Chun Li*. Productive amyrin synthases for efficient α-amyrin synthesis in engineered Saccharomyces cerevisiae. ACS Synth Biol. 2018. 7(10): 2391-402(影响因子5.249,发表年月2018-10) Bo Zheng#, Xiaoyan Ma#, Ning Wang, Tingting Ding, Liwei Guo, Xiaorong Zhang, Yu Yang, Chun Li, Yi-Xin Huo*. Utilization of rare codon-rich markers for screening amino acid overproducers. Nat Commun. 2018. 9(1): 1-11(影响因子14.919,发表年月2018-09) Yi-Xin Huo, Huiyong Ren, Huan Yu, Luyao Zhao, Shengzhu Yu, Yajun Yan, Zhenya Chen*. CipA-mediating enzyme self-assembly to enhance the biosynthesis of pyrogallol in Escherichia coli. Appl Microbiol Biotechnol. 2018. 102(23): 10005-15(影响因子4.813,发表年月2018-09) 彭瑞婷,夏孟丽,茹家康,霍毅欣,杨宇*。聚氨酯塑料的微生物降解。生物工程学报。2018. 34(9): 1398-409(影响因子0.977,发表年月2018-09) Ke Xu#, Bo Lv#, Yi-Xin Huo*, Chun Li*. Toward the lowest energy consumption and emission in biofuel production: combination of ideal reactors and robust hosts. Curr Opin Biotechnol. 2018. 50: 19-24(影响因子9.74,发表年月2018-04) 杨发誉,杨云彭,霍毅欣*。合成生物学中不依赖限制性内切酶的分子克隆策略。中国生物化学与分子生物学报。2018. 34(4): 364-70(影响因子0.812,发表年月2018-04) Yi-Xin Huo, Liwei Guo, Xiaoyan Ma*. Biofuel production with a stress-resistant and growth phase-independent promoter: mechanism revealed by in vitro transcription assays. Appl Microbiol Biotechnol. 2018. 102(6): 2929-40(影响因子4.813,发表年月2018-02) Ming Zhu, Caixia Wang, Wentao Sun, Anqi Zhou, Ying Wang, Genlin Zhang, Xiaohong Zhou, Yi-Xin Huo, Chun Li*. Boosting 11-oxo-β-amyrin and glycyrrhetinic acid synthesis in Saccharomyces cerevisiae via pairing novel oxidation and reduction system from legume plants. Metab Eng. 2018. 45: 43-50(影响因子9.783,发表年月2018-01) Ke Xu, Liman Gao, Jalees Ul Hassan, Zhiping Zhao, Chun Li, Yi-Xin Huo, Guiyan Liu*. Improving the thermo-tolerance of yeast base on the antioxidant defense system. Chem Eng Sci. 2018. 175: 335-42(影响因子4.311,发表年月2018-01) Xiaoxue Wang, Dong Wang, Yi-Xin Huo, Dazhang Dai, Chihua Li, Chun Li, Guiyan Liu*. Identification of isoliquiritigenin as an activator that stimulates the enzymatic production of glycyrrhetinic acid monoglucuronide. Sci Rep-UK. 2017. 7(1): 1-10(影响因子4.38,发表年月2017-10) Yi-Xin Huo. Amino Acid Biosynthesis and Its Metabolic Engineering Applications. Engineering Microbial Metabolism for Chemical Synthesis. 2017: 69-95 Xiaoyan Ma, Yi-Xin Huo*. The application of microfluidic-based technologies in the cycle of metabolic engineering. Syn Syst Biotechno. 2016. 1(3): 137-42(影响因子4.781,发表年月2016-09) Natasha K Vidangos, Johanna Heideker, Artem Lyubimov, Meindert Lamers, Yi-Xin Huo, Jeffrey G Pelton, Jimmy Ton, Jay Gralla, James Berger, David E Wemmer*. DNA recognition by a σ54 transcriptional activator from Aquifex aeolicus. J Mol Biol. 2014. 426(21): 3553-68(影响因子5.469,发表年月2015-10) Yuan-Tao Zhang, Feng Jiang, Zhe-Xian Tian, Yi-Xin Huo, Yi-Cheng Sun, Yi-Ping Wang*. CRP-cyclic AMP dependent inhibition of the xylene-responsive σ54-promoter Pu in Escherichia coli. PLoS One. 2014. 9(1): e86727(影响因子3.24,发表年月2014-01) Yi-Xin Huo, David G Wernick, James C Liao*. Toward nitrogen neutral biofuel production. Curr Opin Biotechnol. 2012. 23(3): 406-13(影响因子9.74,发表年月2012-06) Han Li, Paul H Opgenorth, David G Wernick, Steve Rogers, Tung-Yun Wu, Wendy Higashide, Peter Malati, Yi-Xin Huo, Kwang Myung Cho, James C Liao*. Integrated electromicrobial conversion of CO2 to higher alcohols. Science. 2012. 335(6076): 1596(影响因子47.728,发表年月2012-05) Harsharan Singh Bhatia, Rahul Agrawal, Sandeep Sharma, Yi-Xin Huo, Zhe Ying, Fernando Gomez-Pinilla*. Omega-3 fatty acid deficiency during brain maturation reduces neuronal and behavioral plasticity in adulthood. PloS one. 2011. 6(12): e28451(影响因子3.24,发表年月2011-12) Yi-Xin Huo, Kwang Myung Cho, Jimmy G Lafontaine Rivera, Emma Monte, Claire R Shen, Yajun Yan, James C Liao*. Conversion of proteins into biofuels by engineering nitrogen flux. Nat Biotechnol. 2011. 29(4): 346-51(影响因子54.908,发表年月2011-05) Yi-Xin Huo#, Yuan-Tao Zhang#, Yan Xiao, Xiaodong Zhang, Martin Buck, Annie Kolb, Yi-Ping Wang*. IHF-binding sites inhibit DNA loop formation and transcription initiation. Nucleic Acids Res. 2009. 37(12): 3878-86(影响因子16.971,发表年月2009-04) Jay D Gralla*, Yi-Xin Huo. Remodeling and activation of Escherichia coli RNA polymerase by osmolytes. Biochemistry. 2008. 47(50): 13189-96(影响因子3.163,发表年月2008-11) Yi‐Xin Huo, Adam Z Rosenthal, Jay D Gralla*. General stress response signalling: unwrapping transcription complexes by DNA relaxation via the sigma38 C‐terminal domain. Mol Microbiol. 2008. 70(2): 369-78(影响因子3.501,发表年月2008-09) Xian-Jun Mao#, Yi-Xin Huo#, Martin Buck, Annie Kolb, Yi-Ping Wang*. Interplay between CRP-cAMP and PII-Ntr systems forms novel regulatory network between carbon metabolism and nitrogen assimilation in Escherichia coli. Nucleic Acids Res. 2007. 35(5): 1432-40(影响因子16.971,发表年月2007-02) Yi-Xin Huo, Bei-Yan Nan, Cong-Hui You, Zhe-Xian Tian, Annie Kolb, Yi-Ping Wang*. FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at ?55, upstream of the transcription start site. FEMS Microbiol Lett. 2006. 257(1): 99-105(影响因子2.742,发表年月2006-04) Yi‐Xin Huo#, Zhe‐Xian Tian#, Mathieu Rappas, Jin Wen, Yan‐Cheng Chen, Cong‐Hui You, Xiaodong Zhang, Martin Buck, Yi‐Ping Wang*, Annie Kolb. Protein‐induced DNA bending clarifies the architectural organization of the σ54‐dependent glnAp2 promoter. Mol Microbiol. 2006. 59(1): 168-80(影响因子3.501,发表年月2006-01) Yi‐Xin Huo. Activité de promoteurs dépendant de sigma54 et courbure de l'ADN induite par des protéines/sous la direction de Yi-Ping Wang, Annie Kolb. 2005. Thèse soutenue en co-tutelle. - Thèse de doctorat: Microbiologie: Université de Pékin: 2005 Thèse de doctorat: Microbiologie: Paris 7: 2005

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