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个人简介

工作经历 2016.4-至今 中国农业大学 农学院 副教授/教授 2006.7-2016.3 中国农业大学 资环学院 讲师/副教授 2013.3-2014.2 Iowa State University (美国) 农学系 2009.2-2009.4 Hohenheim University (德国)作物遗传育种 2008.9-2008.10 Palacky University(捷克)生物化学 2007.8-2008.3 Hohenheim University (德国)植物营养学 从事玉米种子生产和分子遗传学研究,通过突变体筛选,QTL(数量性状位点)定位和GWAS(全基因组关联)分析,克隆控制玉米种子发育和萌发的关键基因并开展功能机理研究。揭示了类泛素修饰激活过程、RNA聚合酶组装活性、RNA转录后的成熟、甲基化介导的表观遗传、以及AP2转录因子等在玉米种子发育和萌发过程中的作用机理。在New Phytologist, Plant Cell & Physiology等植物学主流杂志上发表论文50多篇,其中第一或通讯作者SCI论文近30篇;参与颁布农业行业标准、团体标准、地方标准5项;主编出版《种子生产学》教材及专著4部。

研究领域

1)玉米种子活力性状的分子遗传学研究:针对玉米种子发芽能力,特别是低温发芽和储藏耐受性发芽,开展QTL定位和GWAS关联分析,挖掘关键基因,进行功能机理研究 2)玉米种子发育的分子遗传学研究:种子发育异常突变体基因克隆和功能解析 3)高活力玉米种子生产研究:针对不同的生态区域,研究高活力玉米种子生产、收获和加工技术,以及与环境和基因型的互作效应

近期论文

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Combined population transcriptomic and genomic analysis reveals cis-regulatory differentiation of non-coding RNAs in maize Theor Appl Genet SCI 2023.1 J. Lu(卢嘉雯) 中国农业大学 15(并列通讯) The Anthocyanin Accumulation Related ZmBZ1, Facilitates Seedling Salinity Stress Tolerance via ROS Scavenging. Int. J. Mol. Sci. SCI 2022.12 Jie Wang(王洁) 中国农业大学 10(通讯作者) Transcriptome analysis of near-isogenic lines provides novel insights into genes associated with seed low-temperature germination ability in maize (Zea mays L.) Plants SCI 2022.11 Xuhui Li(李旭辉) 中国农业大学 11(通讯作者) Xinmin Hu, Guihua Wang, Xuemei Du, Hongwei Zhang, Zhenxiang Xu, Jie Wang, Guo Chen, Bo Wang, Xuhui Li, Xunji Chen, Junjie Fu, Jun Zheng, Jianhua Wang*, Riliang Gu*, Guoying Wang*. QTL analysis across multiple environments reveals promising chromosome regions associated with yield-related traits in maize under drought conditions. The Crop Journal, 2021, 9:759–766 Quanquan Chen, Jie Zhang, Jie Wang, Yuxin Xie, Yu Cui, Xuemei Du, Li Li, Junjie Fu, Yunjun Liu, Jianhua Wang*, Guoying Wang* and Riliang Gu*. Small kernel 501 (smk501) encodes the RUBylation activating enzyme E1 subunit ECR1 (E1 C-TERMINAL RELATED 1) and is essential for multiple aspects of cellular events during kernel development in maize. New Phytologist, 2021, 230:2337–2354. Weiwei Chen, Yu Cu, Zheyuan Wang, Rongrong Chen, Cheng He, Yan Liu, Xuemei Du,Yunjun Liu, Junjie Fu, Guoying Wang, Jianhua Wang* and Riliang Gu*. Nuclear-Encoded Maturase Protein 3 Is Required for the Splicing of Various Group II Introns in Mitochondria during Maize (Zea mays L.) Seed Development. Plant & Cell Physiology, 2021, 62(2): 293–305. Kaijian Fan, Yixuan Peng, Zhenjing Ren, Delin Li, Sihan Zhen, Stefan Hey, Yu Cui, Junjie Fu, Riliang Gu, Jianhua Wang, Guoying Wang and Li Li. Maize Defective Kernel605 Encodes a Canonical DYW-Type PPR Protein that Edits a Conserved Site of nad1 and Is Essential for Seed Nutritional Quality. Plant Cell Physiol. 2020, 61(11): 1954–1966. Xuemei Du, Xiaoli Wang, Jie Zhang, Sihan Zhen, Yunjun Liu, Junjie Fu, Jianhua Wang, Riliang Gu*, Guoying Wang*. Transcriptome analysis of leafy near-isogenic lines provides molecular insights into floral transition in maize (Zea mays). Plant Breeding, 2020, 139(10):883–891. Quanquan Chen , Ran Huang , Zhenxiang Xu, Yaxin Zhang, Li Li, Junjie Fu, Guoying Wang, JianhuaWang, Xuemei Du*, and Riliang Gu*. Label-Free Comparative Proteomic Analysis Combined with Laser-Capture Microdissection Suggests Important Roles of Stress Responses in the Black Layer of Maize Kernels. International Journal of Molecular Sciences, 2020, 21:1369 Song Guo, Yanhua Chen, Xiaochao Chen, Yanling Chen, Lan Yang, Lifeng Wang, Yusheng Qin, Mingshun Li, Fanjun Chen, Guohua Mi, Riliang Gu* and Lixing Yuan*. Grain Mineral Accumulation Changes in Chinese Maize Cultivars Released in Different Decades and the Responses to Nitrogen Fertilizer. Frontiers in Plant Science, 2020, 10:1662 Li Li, Feng Wang, Xuhui Li, Yixuan Peng, Hongwei Zhang, Stefan Hey, Guoying Wang, Jianhua Wang*, Riliang Gu*. Comparative analysis of the accelerated aged seed transcriptome profiles of two maize chromosome segment substitution lines Plos ONE, 2020, 14(11):e0216977 L Li, X Li, L Li, J Schnable, R Gu, J Wang. QTL identification and epistatic effect analysis of seed size- and weight-related traits in Zea mays L. Molecular Breeding, 2019, 39 (5), 67 GU Ri-liang, HUANG Ran, JIA Guang-yao, YUAN Zhi-peng, REN Li-sha, LI Li, WANG Jian-hua. Effect of mechanical threshing on damage and vigor of maize seed threshed at different moisture contents. Journal of Integrative Agriculture, 2009, 18 (7), 1571-1578 J LI, SA Ali, G XIAO, F CHEN, L YUAN, R GU. Phenotypic characterization and genetic mapping of the dwarf mutant m34 in maize. Journal of Integrative Agriculture, 2019, 18 (5), 948–957 Y Liu, H Zhang, X Li, F Wang, D Lyle, L Sun, G Wang, J Wang, L Li, R Gu. Quantitative trait locus mapping for seed artificial aging traits using an F2: 3 population and a recombinant inbred line population crossed from two highly related maize inbreds. Plant Breeding, 2019, 138 (1), 29-37 Z Wang, L Zhang, C Sun, R Gu, G Mi, L Yuan. Phylogenetic, expression and functional characterizations of the maize NLP transcription factor family reveal a role in nitrate assimilation and signaling. Physiologia plantarum, 2018, 163: 269–281. Y Zhao, Z Liu, F Duan, X An, X Liu, D Hao, R Gu, Z Wang, F Chen, L Yuan. Overexpression of the maize ZmAMT1; 1a gene enhances root ammonium uptake efficiency under low ammonium nutrition. Plant Biotechnology Reports, 2018, 12 (1):47-56 X Li, G Wang, J, L Li, G Jia, L Ren, T Lubberstedt, G Wang, J Wang and R Gu*. QTL Mapping in Three Connected Populations Reveals a Set of Consensus Genomic Regions for Low Temperature Germination Ability in Zea mays L. Frontiers in Plant Science, 2018. 9:65 R Gu, L Li, X Liang, Y Wang, T Fan, Y Wang and J Wang. The ideal harvest time for seeds of hybrid maize (Zea mays L.) XY335 and ZD958 produced in multiple environments,Scientific Reports, 2017, 7: 17537 Z Liu, K Gao, S Shan, R Gu, Z Wang, EJ Craft, G Mi, L Yuan and F Chen. Comparative Analysis of Root Traits and the Associated QTLs for Maize Seedlings Grown in Paper Roll, Hydroponics and Vermiculite Culture System. Frontier in Plant Science, 2017, 8:436. R Gu, F Chen, L Long, H Cai, Z Liu, J Yang, L Wang, H Li, J Li, W Liu, G Mi, F Zhang, L Yuan. Enhancing phosphorus uptake efficiency through QTL-based selection for root system architecture in maize. Journal of Genetic and Genomics, 2016, 43: 663-672 P Li, Z Zhuang, H Cai, S Cheng, AA Soomro, Z Liu, R Gu, G Mi, L Yuan and F Chen. Use of genotype-environment interactions to elucidate the pattern of maize root plasticity to nitrogen deficiency. Journal of Integrative Plant Biology, 2016, 58: 242-253 R Gu, F Chen, B Liu, X Wang, J Liu, P Li, Q Pan, J Pace, AA Soomro, T Lubberstedt, G Mi and L Yuan. Comprehensive phenotypic analysis and quantitative trait locus identification for grain mineral concentration, content, and yield in maize (Zea mays L.). Theory and Applied Genetics, 2015, 128:1777-1789 P Li, F Chen, H Cai, J Liu, P Pan, Z Liu, R Gu, G Mi, F Zhang and L Yuan. A genetic relationship between nitrogen use efficiency and seedling root traits in maize as revealed by QTL analysis. Journal of Experimental Botany, 2015, 66: 3175-3188 S Guo, W Sun, R Gu, B Zhao, L Yuan and G Mi*. Expression of genes related to nitrogen metabolism in maize grown under organic and inorganic nitrogen supplies. Soil Science and Plant Nutrition, 2015, 61: 275-280 X An, F Duan, S Guo, F Chen, L Yuan and R Gu*. Transcriptional regulation of expression of the maize aldehyde dehydrogenase 7 gene (ZmALDH7B6) in response to abiotic stresses. Journal of Integrative Agriculture, 2014, 13(9): 1900-1908 L Zhao, Z Wang, G Mi, L Yuan and R Gu*. Comparative genome analysis of cytokinin biosynthesis genes (IPTS) reveals conserved orthologs cross Poaceae crops. Research on Crops, 2014, 15(1): 38-48 D Zalabak, P Galuszka, K Mrízová, K Podlesáková, R Gu and J Frébortová. Biochemical characterization of the maize cytokinin dehydrogenase family and cytokinin profiling in developing maize plantlets in relation to the expression of cytokinin dehydrogenase genes. Plant Physiology and Biochemistry, 2014, 74:283-293 R Gu, F Duan, X An, F Zhang, N von Wiren and L Yuan. Characterization of AMT-mediated High-affinity Ammonium Uptake in Roots of Maize (Zea mays L.). Plant and Cell Physiology, 2013, 54(9): 1515–1524 L Yuan, R Gu, E Smith-Valle, D Loqué, WB Frommer, N von Wirén. Allosteric Regulation of Transport Activity by Heterotrimerization of Arabidopsis Ammonium Transporter Complexes in Vivo. The Plant Cell, 2013, 25 (3), 974-984 Li Zhao, Ting Liu, Xia An, Riliang Gu*. Evolution and expression analysis of the β-glucosidase (GLU) encoding gene subfamily in maize. Genes & genomics, 2012, 34:179-187 Riliang Gu, Xiaoling Chen, Yuling Zhou, Lixing Yuan*. Isolation and characterization of three maize aquaporin genes, ZmNIP2;1, ZmNIP2;4 and ZmTIP4;4 involved in urea transport. BMB Reports, 2012, 45(2): 96-101 Li Zhao, Lixing Yuan, Riliang Gu*. A 40-bp A/T-rich repressor element involved in organ-dependent transcriptional regulation of ZmGLU1. Plant Cell, Tissue and Organ Culture, 2011, 105:291–298. Riliang Gu, Junjie Fu, Song Guo, Fengying Duan, Zhangkui Wang, Guohua Mi, Fusuo Zhang, Lixing Yuan*. Comparative expression and phylogenetic analysis of maize cytokinin dehydrogenase / oxidase (CKX) gene family. Journal of Plant Growth Regulation, 2010, 29:428-440. Li Zhao, Riliang Gu, Peng Gao, Guoying Wang*. A nonsymbiotic hemoglobin gene from maize, ZmHb, is involved in response to submergence, high-salt and osmotic stresses. Plant Cell Tissue and Organ Culture, 2008, 95:227–237 Juan Bao, Fanjun Chen, Riliang Gu, Guoying Wang, Fusuo Zhang, Guohua Mi. Lateral root development of two Arabidopsis auxin transport mutants aux1-7 and eir1-1, in response to nitrate supplies. Plant Science, 2007, 173(4): 417–425 Riliang Gu, Li Zhao, Ying Zhang, Xiaoping Chen, Juan Bao, Jinfeng Zhao, Zhangying Wang, Junjie Fu, Tingsong Liu, Jianhua Wang, Guoying Wang*. Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Plant Cell Reports, 2006, 25: 1157-1165 常宝学,赵海燕,蔡国强,魏国庆,顾日良,高永泽,李莉,王建华*. 高活力玉米种子生产理论在鑫海1 58中的应用研究. 新疆农业科技,2021, 13-15 李莉,王建华,顾日良,孙群,康定明. 我国种子检验在“一带一路”背景下的发展策略. 种子科技,2020, 38(17):113-115. 高永泽,顾日良,魏国庆,李莉,赵海燕,蔡国强,王建华. 高活力玉米种子生产理论在九圣禾2468中的应用研究. 中国种业,2020,9:58~60 顾日良,王永强,杨丽维,成广雷,王建华 (2017) 不同成熟度京科968 种子活力与种子物理化学特性的关系研究.玉米科学, 25:49-55 任利沙,顾日良,贾光耀,于晓芳,杨晓军,王建华(2017)种子出苗对玉米个体生长和群体产量的影响. 中国农业大学学报,22: 10-15 任利沙,顾日良,贾光耀,田开新,施秀德,王建华(2016)灌浆期控水和施用控释肥对杂交玉米制种产量和种子质量的影响. 中国农业科学 49 :3108-3118 顾日良,陈范骏,张俊伶,袁力行,邹春琴,陈新平,申建波,江荣凤,张福锁(2013) Seminar课程在研究生培养中的重要作用.学位与研究生教育 7:42-44 安霞,库来宝,米国华,陈范骏*,顾日良,袁力行. 玉米根系发育突变体及相关基因研究进展. 植物营养与肥料学报. 2010, 4:5-6. 专著 王建华,顾日良,李莉,孙群. 玉米种子精选分级技术原理和操作指南. 中国农业大学出版社. 2021 顾日良,王建华,孙群,李莉. 玉米种子加工与贮藏技术手册. 中国农业大学出版社. 2018 顾日良,王建华,孙群,李莉. 河西地区杂交玉米种子生产技术手册. 中国农业大学出版社. 2018

学术兼职

2019.8-至今 中国作物学会作物种子专业委员会秘书长

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