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个人简介

刘维全 1963年8月出生,山东省青岛市黄岛区人。预防兽医学博士,中国农业大学生物学院教授,博士研究生导师。 主讲课程 主讲本科生的《蛋白质工程原理》、《动物生物化学》及《动物生物化学实验技术》;研究生《高级动物生物化学》 获奖情况 获得军队科技进步2等奖1项(第4名)和三等奖1项(第1名) 任现职以来,曾先后二次(04、05年度)年终考评为优秀,二次(07、13年度)被评为学校优秀教师 参加两项校教学改革项目:《生物化学研究技术》、《分子生物学》均被评为校重点课程 承担项目及课题 抗菌肽用于奶牛乳房炎的体细胞基因治疗研究,国家自然科学基金课题,主持人,批准号为:30371064。2004.01~2006.12,21万,已经结题 水貂生长激素cDNA基因表达载体的构建及重组腺病毒介导的体细胞转基因研究,国家自然科学基金课题,项目批准号:30571372。2006.01~2008.12。35万。正在进行 家蝇幼虫耐热DNase样活性蛋白不同功能域的分子定位,国家生物学理科基地科研训练项目,指导教师,批准号:J0730639。5.0万元,2008.07~2010.06,正在进行 小鼠卵母细胞摄入并整合外源DNA的分子机理研究,教育部畜牧、兽医科学博士点基金,主持人,项目编号:200800190026。6.0万元 用4-8细胞胚胎注射构建转基因猪,转基因生物新品种培育科技重大专项,2009ZX08006-O1OB,213万。2009.06-2011.12。任务负责人 版纳微型猪近交系肌内脂肪沉积功能基因及特异性调控元件的规模化克隆与功能验证,转基因生物新品种培育科技重大专项,2009ZX08009-140B,80万。2009.06-2011.12。主要参加人 新型重大动物疫病疫苗与诊断试剂创制及生产工艺创新,863课题,2011AA10A213,121.3万元。子课题“动物病毒疫苗抗原大规模提取纯化工艺研究”负责人。2011.01~2015.12 功能性肽产品的创制与应用,2013BAD10B03,支撑计划,2013.01~2017.12。子课题“海洋动物抗菌肽作为畜禽饲料添加剂的研发与利用”,2013BAD10B03-2。25万。正在实施,参加人 猪繁殖力的生理学及相关遗传调控机理,973课题(2014CB138500),2014.01~2018.12。子课题“环境因素导致猪繁殖力降低的网络调控机制”2014CB138505,主要参加人 水貂犬瘟热、病毒性肠炎二联灭活疫苗的研制,校内项目号29015034。山东德利诺生物工程有限公司,2015.06-2018.05。总经费150万元

研究领域

动物生物化学与分子生物学;动物分子病毒性

近期论文

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杨淑艳,刘维全★,江禹,王吉贵,孙绍光,李全,张丽,刘芃芃,灭活禽流感病毒和新城疫病毒联合用于杀伤肿瘤细胞及其机理的初步探讨。生命的化学,2004,24(增刊):72~75。 刘维全,王吉贵,抗菌肽的抗肿瘤作用研究进展。生命的化学,2004,24(增刊):106~110。 刘维全,刘海鹏,江禹等,王本旭,王吉贵,杨淑艳,孙绍光,涂长春,刘芃芃。禽流感病毒、新城疫病毒、经典猪瘟病毒和口蹄疫病毒多联RT-PCR诊断技术的建立。农业生物技术学报,2005,13(1):92~95。 尚丹,刘维全★,孙绍光,王吉贵,李全,张丽,朱宝成,水貂生长激素cDNA的克隆与序列分析。特产研究,2005,27(2):1~4。 栾新红,胡仲明,刘维全,江禹,柳巨雄,王凯。银染DD-PCR法筛选游泳疲劳小鼠肝脏中差异表达基因的初步研究。中国应用生理学杂志,2005,21(3):296~299。 Liu Wei-Quan1,*, Liu Hai-Peng2, Jiang Yu3, Wang Ben-Xu3, Wang Ji-Gui1,Yang Shu-Yan1, Sun Shao-Guang1, Tu Chang-Chun3 and Liu Peng-Peng1,Establishment of multiple RT-PCR diagnostictechniques for Avian influenza virus (AIV), Newcastle disease virus (NDV), Classical swine fever virus (CSFV) and Foot-and-mouth disease virus (FMDV), Chinese Journal of Agricultural Biotechnology,2005,2(3):161–165 刘维全*,王吉贵,李全,杨淑艳,孙绍光,顾玲,洪燕,刘芃芃,卵巢注射法制备转基因动物的初步研究。第五届国际转基因动物学术讨论会,2005,广州,P33~34. 董凤岚,仲明2,孙绍光,王润平2,王吉贵,张鹏,杨淑艳,刘芃芃,张宏福2,刘维全*。利用SiRNA技术进行高水平能量转化鸡的分子育种研究。畜牧与兽医,2006,38(增刊):108。 王思贤,刘芃芃,王吉贵,杨淑艳,孙绍光,宣柏华,王玉茂,刘维全*。抗菌肽用于奶牛乳房炎的体细胞基因治疗。畜牧与兽医,2006,38(增刊):156。 唐文英,王吉贵,杨淑艳,孙绍光,刘芃芃,刘维全*。抗菌肽真核表达载体pVAX1-ABPS1在人工荷瘤裸鼠基因治疗中的效应研究。畜牧与兽医,2006,38(增刊): 徐一,王吉贵,孙绍光,杨淑艳,张鹏,刘芃芃,刘维全*。人胰岛素基因鸡输卵管特异表达载体的构建及转基因鸡的建立。畜牧与兽医,2006,38(增刊):136。 刘晓娟,王吉贵,孙绍光,杨淑艳,洪燕,王思贤,苏小明,尚丹,刘芃芃,刘维全*。 水貂生长激素全基因的克隆及真核表达载体的构建。畜牧与兽医,2006,38(增刊):121。 ShuYan YANG, WeiQuan LIU *, HuanXian CUI, ShaoGuang SUN, JiGui WANG, In vitro induction of apoptosis in tumor cells by inactivated NDV and IAV, Cancer Biotherapy & Radiopharmaceuticals, 2007,Vol 22, No 2, 200-205.(IF1.763) ShuYan YANG, Jigui WANG, Huanxian CUI, Shaoguang SUN, Ling GU, Yan HONG, Weiquan LIU*, Efficient Generation of Transgenic Mice by Direct Intraovarian Injection of Plasmid DNA, Biochemical and Biophysical Research Communications, 22 June 2007, Pages 266-271.(IF 2.855) Gu L, Wang Q, Wang CM, Hong Y, Sun SG, Yang SY, Wang JG, Hou Y, Sun QY, Liu* WQ. Distribution and Expression of Phosphorylated Histone H3 during Porcine Oocyte Maturation. MOLECULAR REPRODUCTION AND DEVELOPMENT. 75:143–149 (2008).(IF 2.379) Shaoguang Sun1,2, Weiquan Liu1,*, Jigui Wang1, Shuyan Yang1, Ling Gu1,Yan Hong1, Dan Shang1, Benxu Wang1, Xiaoming Su1, And Shunzhang Qi1 Endonuclease Activity of Phenol Oxidase From Musca domestica Larvae . 2008,Biol. Bull. 215: 108–114.(IF1.649) Jian LV1,2, Jianwu ZHANG2, Zhi SUN2, Weiquan LIU1, Shishan YUAN2*. Development of Infectious cDNA Clone of Highly Pathogenic PRRSV Variant Associated with “Porcine High Fever Syndrome” 2008, J.of General Virology. 89:2075~2079(IF3.092) Dongyu Zhoua, Jigui Wangb, Weiquan Liub, Rongxiu Liuc,*, Yuehu Peia Detection of CD2 expression in chicken hematogenic embryo yolk 4 sac lymphoid cells prior to thymus genesis. Progress in Natural Science. 2008,18(12):1589-1594(0.535) P. Zhanga, J. G. Wanga, J. Y. Wanb, W. Q. Liua*, Screening efficient siRNAs in vitro as the candidate genes for chicken anti-avian influenza virus H5N1 breeding.2009,Molecular Biology.43( 6): 1–9 (IF0.6) 王吉贵,刘维全,刘芃芃,动物生物化学教学现状及思考,高等农业教育,2009年,第9期51~53 Fuxian Yu, Jigui Wang, Peng Zhang, Yan Hong, Weiquan Liu★, Fusion expression of cecropin B-like antibacterial peptide in Escherichia coli and preparation of its antiserum. 2010, Biotechnology Letters,32(5): 669(IF1.595). Yan Hong, Ji-Gui Wang, Peng Zhang, Fu-Xian Yu, Wei-Quan Liu*, Histopathology and gene expression analysis in mouse post exposed to diethylstilbestrol (DES). 2010, TOXICOLOGY MECHANISMS AND METHODS. 20(3 ): 105-111 Wei Zhang a,b, Xia Wangb, Zhifeng Xiao b, Weiquan Liu a,*, Bing Chen b, Jianwu Dai b,** Mapping of the minimal internal ribosome entry site element in the human embryonic stem cell gene OCT4B mRNA. Biochemical and Biophysical Research Communications.2010,394: 750-754. Kao Zhanga,1 ;QiangHou b,1; Zhenyu Zhong c; Xiujin Li d; Huihui Chen a; Wenyan Li a; Jiexia Wena; Liyue Wanga; Weiquan Liub?; Fei Zhonga?. Porcine reproductive and respiratory syndrome virus activates inflammasomes of porcine alveolar macrophages via its small envelope protein E. 2013, Virology, 442(2): 156–162 Jia-zeng Sun; Jigui Wang; Daoli Yuan; Shuang Wang; Zhili Li; Bao Yi; Yaping Mao; Qiang Hou; Weiquan Liu*. Cellular microRNA miR-181b Inhibits Replication of Mink Enteritis Virus by Repression of Non-Structural Protein 1 Translation. 2013, PLOS ONE, 8(12):1-14 Yuan D; Wang J; Li Z; Mao Y; Sun JZ; Xi J; Wang S; Hou Q; Yi B; Liu W*. Establishment of a rescue system for an autonomous Parvovirus mink enteritis virus. 2014, Virus Res. 183:1-5 Jia-zeng Sun; Jigui Wang; Shuang Wang; Daoli Yuan; Basse Mame Birame; Zhili Li; Bao Yi; Weiquan Liu*. MicroRNA profile analysis of a feline kidney cell line before and after infection with mink enteritis virus. 2014, Gene, 539(2): 224-229 Jia-zeng Sun; Jigui Wang; Daoli Yuan; Shuang Wang; Zhili Li; Bao Yi; Yaping Mao; Qiang Hou; Weiquan Liu*. MicroRNA miR-320a and miR-140 inhibit mink enteritis virus infection by repression of its receptor, feline transferrin receptor. 2014, Virology Journal, 11:210 Shuang Wang1; Peng Zhang2; Fei He1; Ji-Gui Wang1; Jia-Zeng Sun1; Zhi-Li Li1; Bao Yi1; Ji Xi1; Ya-Ping Mao1; Qiang Hou1; Dao-Li Yuan1; Zi-Ding Zhang1 and Wei-Quan Liu1* Combination of Specific Single Chain Antibody Variable Fragment and siRNA has a Synergistic Inhibitory Effect on the Propagation of Avian Influenza Virus H5N1 in Chicken Cells. 2014, Virology Journal, 11:208 Bao Yi; Jigui Wang; Shuang Wang; Daoli Yuan; Jiazeng Sun; Zhili Li; Yaping Mao; Qiang Hou and Weiquan Liu*. Overexpression of Banna mini-pig inbred line fatty acid binding protein 3 promotes adipogenesis in 3T3-L1 preadipocytes. 2014, Cell Biology International, 38(8):918-923 Basse Mame Birame; Wang Jigui; Yu Fuxian; Sun Jiazeng; Li Zhili; and Liu Weiquan*. Potentiation of Apoptin-Induced Apoptosis by Cecropin B-Like Antibacterial Peptide ABPs1 in Human HeLa Cervical Cancer Cell Lines is Associated with Membrane Pore Formation and Caspase-3 Activation. 2014, J. Microbiol. Biotechnol. , 24(6):756-764 水貂阿留申病毒的结构与功能研究进展,中国毛皮动物研究进展,2014年全国毛皮动物专业学生研讨会论文集,吉林科学技术出版社,P146-151。 Zhili Li, Jigui Wang, Daoli Yuan, Shuang Wang, Jiazeng Sun, Bao Yi, Qiang Hou, Yaping Mao, Weiquan Liu. A recombinant canine distemper virus expressing a modified rabies virus glycoprotein induces immune responses in mice. Virus Genes, 2015, 50(3): 434-441. 王熙 耿圆圆 侯蔷 李智丽 王吉贵 姜伟* 刘维全*。细菌磁小体作为新型非病毒载体用于肿瘤基因治疗的研究进展。肿瘤学杂志,2016,22(4):322~326. Qiang Hou, Jun Su, Jigui Wang, Zhili Li, Yaping Mao, Shuang Wang, Ji Xi, Weiquan Liu*. The phosphorylation of Ser221 in VP2 of mink enteritis virus and its roles in virus amplification. Virus Research, 2016, 217, 76-84. Ji Xi, Jigui Wang, Yongle Yu, Xiaomei Zhang, Yaping Mao, Qiang Hou, Weiquan Liu*. Genetic characterization of the complete genome of an Aleutian mink disease virus isolated in north China. Virus Genes, 2016, 52:463–473 (doi: 10.1007/s11262-016-1320-3). Yaping Mao, Jigui Wang, Qiang Hou, Ji Xi, Xiaomei Zhang, Dawei Bian, Yongle Yu, Xi Wang, Weiquan Liu*. Comparison of biological and genomic characteristics between a newly isolated mink enteritis parvovirus MEV-LHV and an attenuated strain MEV-L. Virus Genes (2016)52:388–396(doi: 10.1007/ s11262-016-1314-1). Yaping Mao, Jun Su, Jigui Wang, Xiaomei Zhang, Qiang Hou, Dawei Bian, Weiquan Liu★ Roles of three amino acids of capsid proteins in mink enteritis parvovirus replication. Virus Research 222 (2016): 24–28. Xiaomei Zhang, Jigui Wang, Yaping Mao, Ji Xi, Yongle Yu, Weiquan Liu* Induction and suppression of type I interferon responses by mink enteritis virus in CRFK cells. Veterinary Microbiology, 199 (2017) 8–14.

学术兼职

全国动物生理生化学会常务理事,副秘书长 中国生物化学与分子生物学学会农业生物化学与分子生物学分会理事 北京市生物化学与分子生物学学会理事 北京市畜牧兽医学会理事 中国畜牧兽医学会会员 中国兽医传染病学会会员 中国生物化学与分子生物学学会会员

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