陈三凤 - 中国农业大学

个人简介

陈三凤博士，教授。1992年在中国农业大学获得农学博士学位。主要从事生物固氮研究工作。先后主持9项国家自然基金，主持、课题主持和子课题主持博士点基金、863课题、973子课题、转基因专项、重点研发等多项科研项目。发表论文100余篇，其中SCI论文80余篇。近年来，3篇论文发表在PloS Genetics；申请和获得国家发明专利授权13项。先后主讲《微生物遗传学》、《微生物生理学》、《生物固氮调控机制》等课程。编写和参编教材和科技著作5部，其中编写的《现代微生物遗传》获“北京市精品教材奖”和“国家十一五规划教材奖”。科研成果获北京市自然科学三等奖。先后任中国微生物学会理事、北京市微生物学会理事和农业微生物专业委员会副主任委员、中国微生物学会普及与教育工作委员会委员。学习与工作经历 1978/10-1982/08，山西农业大学植物保护系，农学学士学位 1986/08-1989/08，南京农业大学植物病理系，农学硕士学位 1989/09-1992/06，中国农业大学植物病理系，农学博士学位 1995/04-1996/04，墨西哥理工大学，博士后 1996/06-1997/08，日本食品研究所，博士后 2003/10-2004/04，美国亚利桑那大学，高级访问学者 2011/06-2011/12，美国乔治亚大学，高级访问学者 1982/08-1986/08，山西农业大学植物病理系，助教 1992/07-至今，中国农业大学生物学院，讲师、副教授和教授获奖情况科研成果“固氮微生物资源、基因组序列分析及遗传研究”，2016年获北京市自然科学三等奖编著的《现代微生物遗传》于2006年，获“北京市精品教材”奖和“十一五国家规划教材” 参讲的研究生课程《现代微生物研究技术》，获中国农业大学教学优秀成果奖 2008年获中国农业大学教学成果二等奖指导的2名硕士生获中国农业大学优秀论文奖

研究领域

固氮微生物能将空气中的氮气还原成氨，为植物生长提供一定量的氮素。固氮微生物包括不同的种类，研究较多的是革兰氏阴性固氮菌。类芽孢杆菌（Paenibacillus）是产生芽孢的革兰氏阳性细菌，其中包括一些固氮菌。我的研究主要集中在这几个方面：一、固氮类芽孢杆菌资源分类；二、固氮调控机制研究和利用合成生物学方法研究固氮基因的表达；三、固氮菌的发酵、微生物肥料制备及其田间应用

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Wang Tianshu, Zhao Xiyun, Shi Haowen, Sun Li, Li Yongbin, Li Qin, Zhang Haowei, Chen Sanfeng * and Jilun Li.(2018) Positive and negative regulation of transferred nif genes mediated by indigenous GlnR in Gram-positive Paenibacillus polymyxa. PloS Genetics (in press). Li Yongbin, Liu Xiaomeng, Hao Tianyi and Chen Sanfeng*(2017) Colonization and maize growth promotion induced by phosphate solubilizing bacterial isolates. International Journal of molecular science. 18, 1253; doi:10.3390/ijms18071253. Hao Tianyi and Chen Sanfeng* (2017) Colonization of wheat, maize and cucumber by Paenibacillus polymyxa WLY78. Plos One 12(1): e0169980. Zhou Jing, Jiang Xin, Zhou Baoku, Zhao Baisuo, Ma Mingchao, Chen Sanfeng, Cao Fengming, Shen Delong, Guan Dawei and LiJun (2017) Consistent effects of nitrogen fertilization on soil bacterial communities in black soils for two crop seasons in China. Scientific Reports | 7: 3267 | DOI:10.1038/s41598-017-03539-6. Ma Rui, Chen Qian, Fan Yunliu, Wang Qi, Chen Sanfeng, Liu Xingzhong , Cai Lei and Yao Bin (2017) Six new soil–inhabiting Cladosporium species from plateaus in China. Mycologia. 109(2):244-260. Xie Jianbo, Shi Haowen, Du Zhenglin, Wang Tianshu, Liu Xiaomeng and Chen Sanfeng* (2016) Comparative genomic and functional analysis reveal conservation of plant growth promoting traits in Paenibacillus polymyxa and its closely related species. Scientific Reports. 6: 21329. DOI: 10.1038/srep21329. Li Xinxin, Liu Qi, Liu Xiaomeng, Haowen and Chen Sanfeng* (2016) Using synthetic biology to increase nitrogenase activity. Microbial Cell Factories. 15: 43-44. Shi Haowen, Wang Liying, Li Xinxin, Liu Xiaomeng, Hao Tianyi, He Xiaojuan and Chen Sanfeng*（2016）Genome-wide transcriptome profiling of nitrogen fixation in Paenibacillus sp. WLY78. BMC Microbiology. 16:25-35, DOI 10.1186/s12866-016-0642-6. Zhou Jing, Jiang Xin, Zhou Baoku, Zhao Baisuo, Ma Mingchao, Guan Dawei, Qin Jie, Chen Sanfeng, Cao Fengming, Shen Delong and Li Jun (2016) Thirty four years of nitrogen fertilization decreases fungal diversity and alters fungal community composition in black soil in northeast China, Soil Biology and Biochemistry.95: 135-143. Xiong Lili，Ayyappa Kumar Sista Kameshwar, Chen Xi, Guo Zhiyun, Mao Canquan, Chen Sanfeng and Qin Wensheng (2016) The ACEII recombinant Trichoderma reesei QM9414 strains with enhanced xylanase production and its applications in production of xylitol from tree barks. Microb Cell Fact.15:215-233，DOI 10.1186/s12934-016-0614-4 Zhang Lihong and Chen Sanfeng* (2015) Influence of different factors on the nitrogenase activity of the engineered Escherichia coli 78-7. World J Microbiol Biotechnol. 31: 921–927. Wang Liying, Wang Tianshu and Chen Sanfeng* (2015) Cohnella capsici sp. nov., a novel nitrogen-fixing species isolated from Capsicum annuum rhizosphere soil, and emended description of Cohnella plantaginis. Antonie van Leeuwenhoek. 107: 133–139. Zhang Lihong, Liu Xiaomeng, Li Xinxin and Chen sanfeng* (2015) Expression of the N2 fixation gene operon of Paenibacillus sp. WLY78 under the control of the T7 promoter in Escherichia coli BL21. Biotechnol Letter. 37: 1999–2004. Zhang Lihong and Chen Sanfeng* (2015) Influence of different factors on the nitrogenase activity of the engineered Escherichia coli 78-7. World J Microbiol Biotechnol. 31:921–927. Zhang bo and Chen sanfeng* (2015) Effect of different organic matters on flocculation of Chlorella sorokiniana and optimization of flocculation conditions in swine manure wastewater. Bioresource Technology. 192: 774–780. Zhang bo and Chen sanfeng* (2015) Optimization of culture conditions for Chlorella sorokiniana using swine manure wastewater. Journal of Renewable and Sustainable Energy. 7: 19554-279. Yin Zhongwei, Shi Fachao, Jiang Hongmei, Daniel P. Roberts, Chen Sanfeng and Fan Bingquan* (2015). Phosphate solubilization and promotion of maize growth by Penicillium oxalicum P4 and Aspergillus niger P85 in a calcareous soil. Canadian Journal of Microbiology. 61: 913-23. Zhou Jing, Guan Dawei , Zhou Baoku, Zhao Baisuo , Ma Mingchao, Qin Jie , Jiang Xin, Chen Sanfeng, Cao Fengming, Shen Delong, Li Jun* (2015) Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China. Soil Biology & Biochemistry. 90: 42-51. Xie Jianbo, Du Zhenglin, Bai Lanqing, Tian Changfu, Zhang Yunzhi, Xie Jiuyan, Wang Tianshu, Liu Xiaomeng, Chen Xi, Cheng Qi*, Chen Sanfeng* and Li Jilun (2014) Comparative genomic analysis of N2-fixing and non-N2-fixing Paenibacillus spp.: organization, evolution and expression of the nitrogen fixation genes. PLoS Genetics.10(3): e1004231. doi:10.1371. Li Xinxin, Deng Zhiping, Liu Zhanzhi, Yan Yongliang, Wang Tianshu, Xie Jianbo, Lin Min, Cheng Qi and Chen Sanfeng* (2014). The Genome of Paenibacillus sabinae T27: Evolution, Organization, and heterologous expression of nitrogen fixation gene cluster. BMC Genomics. 15:723-737. Hong Yuanyuan, Mehdi Dashtban, Greg Kepka, Chen Sanfeng and Qin Wensheng * (2014). Overexpression of D-Xylose Reductase (xyl1) Gene and antisense inhibition of D-Xylulokinase (xyiH) gene increase Xylitol production in Trichoderma reesei. BioMed Research International. ID 169705. Liu Yu, Wang Shuang, Zhang D, Wei S, Zhao S, Chen Sanfeng, Xu Feng* (2013) Pantoea beijingensis sp. nov., isolated from the fruiting body of Pleurotus eryngii. Antonie van Leeuwenhoek.104:1039-1047. Wang Liying, Zhang Lihong, Liu Zhanzhi, Zhao Dehua, Liu Xiaomeng, Zhang Bo, Xie Jianbo, Hong Yuanyuan, Li Pengfei, Chen Sanfeng*, Ray Dixon*, and Li Jilun (2013) A minimal nitrogen fixation gene cluster from Paenibacillus sp. WLY78 enables expression of active nitrogenase in Escherichia coli. PLoS Genetics. 9: 9（10）e1003865. Wang Liying, Li Ji, Li Qing X., Chen Sanfeng* (2013) Paenibacillus beijingensis sp. nov., a nitrogen-fixing species isolated from wheat rhizosphere soil. Antonie van Leeuwenhoek. 104: 675-683.

学术兼职

北京市微生物学会理事中国微生物学会农业微生物专业委员会委员