Affourtit, Charles - Plymouth University - School of Biomedical and Healthcare Sciences

个人简介

Positions Plymouth University - UK Reader in Mitochondrial Biology, 2016- Lecturer in Biochemistry, 2010-2016 Seahorse Bioscience - Billerica USA Scientific consultant, 2009-2010 Buck Institute for Age Research - North-Bay Area USA Visiting Scientist, 2009-2010 MRC Mitochondrial Biology Unit - Cambridge UK Investigative Scientist with Prof. Martin Brand, 2004-2009 University of Sussex - Brighton UK Post-Doctoral Research Associate with Prof. Tony Moore, 1999-2004 Degrees PGCert in Academic Practice Plymouth University, 2011 DPhil in Biochemistry University of Sussex, 2000 MSc in Biology Vrije Universiteit Amsterdam, 1995

研究领域

Generally, I am intruiged by the role of bioenergetic failure in disease pathophysiology. In particular, I wish to understand how mitochondrial dysfunction affects glucose-stimulated insulin secretion by pancreatic beta cells and the insulin responsiveness of skeletal muscle. Defects in insulin secretion and action that arise in obesity are both features of the Metabolic Syndrome, a cluster of medical disorders that collectively increase the risk of developing type 2 diabetes. My current projects focus on (1) the physiological role of mitochondrial uncoupling protein-2 in beta cells, (2) mitochondrial involvement in beta cell glucolipotoxicity, (3) the bioenergetics of fatty acid-induced insulin resistance of skeletal muscle, and (4) the effect of dietary nitrate on skeletal muscle function.

近期论文

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Affourtit C 2016 'Mitochondrial involvement in skeletal muscle insulin resistance: A case of imbalanced bioenergetics' BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1857, (10) 1678-1693 Author Site , DOI PEARL Nisr RB & Affourtit C 2016 'Palmitate-induced changes in energy demand cause reallocation of ATP supply in rat and human skeletal muscle cells' BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1857, (9) 1403-1411 Author Site , DOI PEARL Barlow J, Jensen VH, Jastroch M & Affourtit C 2016 'Palmitate-induced impairment of glucose-stimulated insulin secretion precedes mitochondrial dysfunction in mouse pancreatic islets' BIOCHEMICAL JOURNAL 473, 487-496 Author Site , DOI PEARL Hirschberg Jensen V & Affourtit C 2015 'Mitochondrial uncoupling protein-2 is not involved in palmitate-induced impairment of glucose-stimulated insulin secretion in INS-1E insulinoma cells and is not needed for the amplification of insulin release' Biochemistry and biophysics reports 1, 8-15 , DOI PEARL Affourtit C, Bailey SJ, Jones AM, Smallwood MJ & Winyard PG 2015 'On the mechanism by which dietary nitrate improves human skeletal muscle function' FRONTIERS IN PHYSIOLOGY 6, Author Site , DOI PEARL Barlow J, Hirschberg Jensen V & Affourtit C 2015 'Uncoupling protein-2 attenuates palmitoleate protection against the cytotoxic production of mitochondrial reactive oxygen species in INS-1E insulinoma cells' Redox Biol 4, 14-22 Author Site , DOI PEARL Nisr RB & Affourtit C 2014 'Insulin acutely improves mitochondrial function of rat and human skeletal muscle by increasing coupling efficiency of oxidative phosphorylation' Biochim Biophys Acta 1837, (2) 270-276 Author Site , DOI PEARL Barlow J, Hirschberg V, Brand MD & Affourtit C 2013 'Measuring mitochondrial uncoupling protein-2 level and activity in insulinoma cells' Methods Enzymol 528, 257-267 Author Site , DOI Barlow J & Affourtit C 2013 'Novel insights into pancreatic β-cell glucolipotoxicity from real-time functional analysis of mitochondrial energy metabolism in INS-1E insulinoma cells' Biochem J 456, (3) 417-426 Author Site , DOI PEARL