SHEN, HAO - Kent State University - Department of Chemistry and Biochemistry

个人简介

Professional Appointments 2018- Assistant professor, Kent State University Education and Training 2014 – 2018 Postdoctoral Associate, Department of Chemistry, Rice University Protein-Synthetic Polymer Interactions Advisor: Christy F. Landes 2008 – 2014 Ph.D., Chemistry and Chemical Biology, Cornell University Chemical and Electrochemical Catalysis Advisor: Peng Chen 2003 – 2007 B.S. Chemistry and Chemical Engineering, Nanjing University Heterogeneous Catalysis Advisor: Yuchuan Fu

研究领域

The Shen group focuses on utilizing state-of-the-art single-molecule spectroscopy to investigate the properties of polymeric materials and their potential applications in catalysis and bioseparation. Unlike conventional ensemble measurements, single-molecule spectroscopy analyzes one probing molecule at a time to reveal the performances dictating subpopulations within a system. Its subdiffraction limited localization enables the microscopic to mesoscopic mapping. Moreover, the real-time observation under millisecond resolution enables the dynamic study of complex processes. With this main approach, the Shen group aims to study the following. 1. Developing advanced microscopy approaches. Thanks to the developments of fluorescence microscopy, researchers nowadays have a non-destructive approach to observe many soft matters and biological systems in-situ. The persistent efforts in the past two decades have broken the diffraction limits, allowing an optical microscope to achieve nanometer precisions. We now understand many systems better than ever. However, the needs for more capable observation tools will never stop. The Shen group will use phase modulations to develop novel imaging methods that enable higher precision and wider observation ranges. 2. Spatially resolved polymerization kinetics. The world polymer production surpassed 300 megatons in 2015 and is still expanding rapidly. However, a detailed description of the polymerization kinetics lacks because there are some “anomalous” behaviors associated with the reaction types, such as auto-acceleration, radical trapping, gelation, and diffusion induced termination. Such anomalous processes usually result in severe polydispersity in chain sizes within the bulk polymer materials, ultimately downgrading the performances of functional polymers. The Shen group strives to understand how micro-environment affects the polymerization kinetics. The goal is to understand the polymerization mechanisms better and design new strategies to prepare more homogeneous polymers. 3. Synthetic polymer membranes for protein separations. Proteins carry out vital physiological activities that are widely used in the pharmaceutical and food industries. Each protein exhibits unique physiological functionalities requiring high purity and concentrations when used for manufacturing highly specific products. Chromatography utilizing synthetic polymers as the stationary phase materials is the industrial standard in downstream processing, which may account for as much as 50% of the overall cost to bring a protein product to the market. Consequently, it is critical to understand the dynamics of protein-synthetic polymer interactions, because any such insights will in turn benefit the designing of high-efficient, anti-fouling stationary phase materials to reduce the manufacturing costs for protein-based products. The Shen group uses single-molecule spectroscopy to map out the protein-polymer interactions in real-time.

近期论文

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L. Zuo, K. Ren, X. Guo, P. Pokhrel, B. Pokhrel, M.A. Hossain, Z. Chen, H. Mao, H. Shen, “Amalgamation of DNAzyme and Nanozyme in DNA Corona Phase”, Journal of the American Chemical Society, doi.org/10.1021/jacs.2c12367. P. Pokhrel, K. Ren, H. Shen, H. Mao, “Mechanical Stability of DNA Corona Phase on Gold Nanospheres”, Langmuir, 2022, 38, 13569-13576. L. Zuo, M.A. Hossain, B. Pokhrel, W.-S. Chang, H. Shen, “Catalysis Driven by Biohybrid Nanozyme”, Advanced Sensor and Energy Materials, 2022, 1, 100024. A. Misiura, H. Shen, L. Tauzin, C. Dutta, L.D.C. Bishop, N.C. Carrejo, J. Zepeda O, S. Ramezani, N.A. Moringo, A.B. Marciel, P.J. Rossky, C.F. Landes, “Single-Molecule Dynamics Reflect IgG Conformational Changes Associated with Ion-Exchange Chromatography”, Analytical Chemistry, 2021, 93, 11200-11207. R. Song, B. Yu, D. Friedrich, J. Li, H. Shen, H. Krautscheid, S. Huang, and M.H. Kim, “Naphthoquinone-derivative as a synthetic compound to overcome resistance in MRSA”, Communications Biology, 2020, 3, 1-11. N.A. Moringo, H. Shen, L.J. Tauzin, W. Wang, C.F. Landes, “Polymer Free volume Effects on Protein Dynamics at Polystyrene Revealed by Single-Molecule Spectroscopy”, Langmuir, 2020, 36, 2330–2338. N.A. Moringo, L.D.C. Bishop, H. Shen, A. Misiura, N.C. Carrejo, R. Baiyasi, W. Wang, F. Ye, J.T. Robinson, C.F. Landes, “A Mechanistic Examination of Salting out in Protein-Polymer Membrane Interactions”, Proc. Natl. Acad. Sci. U.S.A., 2019,116, 22938-22945. A.C. Dassanayake, N.P. Wickramaratne, M.A. Hossain, V.S. Perera, J. Jeskey, S.D. Huang, H. Shen, M. Jaroniec, “Prussian Blue-Assisted One-Pot Synthesis of Nitrogen-Doped Mesoporous Graphitic Carbon Spheres with Embedded Iron Oxide”, Journal of Materials Chemistry A, 2019, 7, 22092-22102. W. Wang, F. Ye, H. Shen, N.A. Moringo, C. Dutta, J.T. Robinson, C.F. Landes, ” Generalized Method to Design Phase Masks for 3D Super-Resolution Microscopy”, Optical Express, 2019, 27, 3799-3816. W. Wang, H. Shen, N.A. Moringo, N.C. Carrejo, F. Ye, J.T. Robinson, C.F. Landes, “Super-Temporal Resolved Microscopy Reveals Multistep Desorption Kinetics of α-Lactalbumin from Thin Nylon”, Langmuir, 2018, 34, 6697-6702. N.A. Moringo, H. Shen, L.D.C. Bishop, W. Wang, C.F. Landes, “Enhancing Analytical Separations using Super Resolution Microscopy”, Annual Review of Physical Chemistry, 2018, 69, 353-375. N. Zou, G. Chen, X. Mao, H. Shen, E. Choudhary, X. Zhou, P. Chen, “Imaging Catalytic Hotspots on Single Plasmonic Nanostructures via Correlated Super-Resolution and Electron Microscopy”, ACS Nano, 2018, 12, 5570-5579. N.A. Moringo, H. Shen, L.J. Tauzin, W. Wang, L.D.C. Bishop, C.F. Landes, “Variable Lysozyme Transport Dynamics on Oxidatively Functionalized Polystyrene Films”, Langmuir, 2017, 33, 10818-10828. H. Shen, L.J. Tauzin, R. Baiyasi, W. Wang, N.A. Moringo, B. Shuang, C.F. Landes, “Single Particle Tracking: From Theory to Biophysical Applications”, Chemical Reviews, 2017, 117, 7331-7376. W. Wang, H. Shen, B. Shuang, B.S. Hoener, L.J. Tauzin, N.A. Moringo, K.F. Kelly, C.F. Landes, “Super Temporal-Resolved Microscopy (STREM)”, The Journal of Physical Chemistry Letters, 2016, 7, 4524-4529. B. Shuang, W. Wang, H. Shen, L. Tauzin, C. Flatebo, J. Chen, K.F. Kelly, C.F. Landes, “Generalized Recovery Algorithm for 3D Super-Resolution Microscopy using Rotating Point Spread Functions”, Scientific Reports, 2016, 6, 30826. H. Shen, L. Tauzin, W. Wang, B. Hoener, B. Shuang, L. Kisley, A. Hoggard, C.F. Landes, “Single-Molecule Kinetics of Protein Adsorption on Thin Nylon 6,6 Films”, Analytical Chemistry, 2016, 88, 9926-9933. L. Tauzin, H. Shen, N.A. Moringo, M.H. Roddy, C.A. Bothof, G.W. Griesgraber, A.K. McNulty, J.K. Rasmussen; C.F. Landes, “Variable Surface Transport Modalities on Functionalized Nylon Films Revealed with Single-Molecule Spectroscopy”, RSC Advances, 2016, 6,27760-27766. H. Shen, X. Zhou, N. Zou, P. Chen, “Single-Molecule Kinetics Reveals a Hidden Surface Reaction Intermediate in Single-Nanoparticle Catalysis”, Journal of Physical Chemistry C, 2014, 118, 26902-26911. P. Chen, X. Zhou, N.M. Andoy, K.-S. Han, E. Choudhary, N. Zou, G. Chen, H. Shen, “Spatiotemporal Catalytic Dynamics within Single Nanocatalysts Revealed by Single-Molecule Microscopy”, Chemical Society Review, 2014, 43, 1107-1117.