Milton, Ross - University of Geneva - Department of Inorganic and Analytical Chemistry

个人简介

Biography: Ross was raised in the south of the United Kingdom. Following on from a childhood interest in understanding "how things work", Ross completed a BSc (2010) and PhD (2014) in Chemistry at the University of Surrey (UK), where his PhD thesis focused on enzymatic electrodes in glucose-oxidizing fuel cells for energy conversion under the supervision of Robert Slade and Alfred Thumser. Ross then began his first postdoctoral position in Shelley Minteer's group at the University of Utah (USA), first focusing on alternative electrode constructs and new materials (such as redox polymers) for energy conversion by enzymatic electrodes. During this time, Ross developed an interest in the concept of enzymatic electrosynthesis whereby electrical energy is consumed to produce valuable chemical commodities at enzymatic electrodes. He was awarded a Marie Skłodowska-Curie Individual Fellowship (2015) between the University of Utah and the National University of Ireland Galway (Dónal Leech, incoming phase) to investigate the electroenzymatic reduction of unreactive dinitrogen to ammonia by nitrogenase ("Bioelectroammonia", Grant ID: 654836). With a developing interest in complex enzymatic electron transfer and catalytic mechanisms, Ross embarked upon a second postdoctoral position (2017) in Alfred Spormann's group at Stanford University (USA). During this time, he investigated flavin-based electron bifurcation by large metalloenzyme complexes (heterodisulfide reductase) in the methanogenic archaeon Methanococcus maripaludis. After completing the incoming phase of the MSC fellowship in Dónal Leech's group, Ross began his independent career as an Assistant Professor (tenure track) in the Department of Inorganic and Analytical Chemistry at the University of Geneva (Switzerland) in September 2019.

研究领域

Research in the Milton Group seeks to couple metalloenzymes that catalyze important reductive reactions to electrodes, in order to study their electron transfer/catalytic mechanisms and ultimately aid the development of new biotechnologies (and bio-inspired technologies). Our multidisciplinary research combines skills ranging from recombinant protein production to enzymatic electrochemistry to study enzymes that catalyze reactions such as (i) dinitrogen (N2) reduction to ammonia (NH3), (ii) carbon dioxide (CO2) reduction, and (iii) large metalloenzyme complexes that employ non-trivial electron transfer mechanisms. In enzymatic electrochemistry, electrodes can be electronically coupled to metalloenzymes in many different ways although the desired outcome remains the same: the electrode supplies the reducing equivalents to the reductive metalloenzyme for subsequent catalysis (or, electrocatalysis) where the corresponding current is proportional to the rate of substrate reduction by the enzyme.

近期论文

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“Facile functionalization of carbon electrodes for efficient electroenzymatic hydrogen production”Liu, Y.*, Webb, S., Moreno-García, P., Kulkarni, A., Maroni, P., Broekmann, P. and Milton, R.D.* (2022).JACS Au, In Press DOI: 10.1021/jacsau.2c00551 “Enzymatic and microbial electrochemistry: Approaches and Methods”Bedendi, G., De Moura Torquato, L.D., Webb, S., Cadoux, C., Kulkarni, A., Sahin, S., Maroni, P., Milton, R.D.* and Grattieri, M.* (2022).ACS Measurement Science Au, 2(6), 517 - 541 DOI: 10.1021/acsmeasuresciau.2c00042 “Alternative electron donors for the nitrogenase-like dark operative protochlorophyllide oxidoreductase (DPOR)”Bedendi, G., Kulkarni, A., Maroni, P. and Milton, R.D.* (2022).ChemElectroChem, 9(21), Article: e202200774 DOI: 10.1002/celc.202200774 “Peroxidase activity of myoglobin variants reconstituted with artificial cofactors”Guo, C., Chadwick, R.J., Foulis, A., Bedendi, G., Lubskyy, A. Rodriguez K.J., Pellizzoni, M., Milton, R.D., Beveridge, R. and Bruns, N.* (2022).ChemBioChem, 23(18), article e202200197 DOI: 10.1002/cbic.202200197 “Evolving enzymatic electrochemistry with rare or unnatural amino acids”Sahin, S* and Milton, R.D.* (2022).Current Opinion in Electrochemistry, 35, article 101102 DOI: 10.1016/j.coelec.2022.101102 “Nitrogenase loosens its belt to fix dinitrogen”Milton, R.D.* (2022).Nature Catalysis, 5, 361-362. DOI: 10.1038/s41929-022-00795-2, Free to read here. “Following Electroenzymatic Hydrogen Production by Rotating Ring Disk Electrochemistry and Mass Spectrometry”Khushvakov, J., Nussbaum, R., Cadoux, C., Duan, J., Stripp, S.T. and Milton, R.D.* (2021).Angewandte Chemie International Edition, 60(18), 10001-10006. DOI: 10.1002/anie.202100863 “Natural and Engineered Electron Transfer of Nitrogenase”Gu, W. and Milton, R.D.* (2020).Chemistry, 2(2), 322-346.DOI: 10.3390/chemistry2020021 “Recent Enzymatic Electrochemistry for Reductive Reactions”Cadoux, C. and Milton, R.D.* (2020).ChemElectroChem, 7(9):1974-1986 DOI: 10.1002/celc.202000282 “Nitrogenase Bioelectrochemistry for Electrosynthesis Applications” Milton, R.D.* and Minteer, S.D.* (2019). Accounts of Chemical Research, 51(12):3351-3360. DOI: 10.1021/acs.accounts.9b00494 “Enhanced Electrosynthetic Hydrogen Evolution by Hydrogenases Embedded in a Redox-Active Hydrogel”Ruth, J.C., Milton, R.D., Gu, W. and Spormann, A.M.* (2020).Chemistry - A European Journal, 26(32):7323-7329. DOI: 10.1002/chem.202000750 “Pyrene-based Noncovalent Immobilization of Nitrogenase on Carbon Surfaces” Patel, J., Cai, R., Milton, R.D., Chen, H. and Minteer, S.D.* (2019). ChemBioChem, 21(12):1729-1732. DOI: 10.1002/cbic.201900697 “Microbial Battery Powered Enzymatic Electrosynthesis for Carbon Capture and Generation of Hydrogen and Formate from Dilute Organics”Dubrawski, K.L., Shao, X., Milton, R.D., Deutzmann, J.S., Spormann, A.M.* and Criddle, C.S.* (2019).ACS Energy Letters, 4(12):2929-2936. DOI: 10.1021/acsenergylett.9b02203 “Efficient NADH Regeneration by a Redox Polymer-Immobilized Enzymatic System”Yuan, M., Kummer, M., Milton, R.D., Quah, T. and Minteer, S.D.* (2019).ACS Catalysis, 9(6):5486-5495 “Following Nature: Bioinspired Mediation Strategy for Gram-Positive Bacterial Cells”Pankratova, G., Pankratov, D., Milton, R.D., Minteer, S.D. and Gorton, L.* (2019).Advanced Energy Materials, 9(16):1900215 “Performance Comparison of Different Configurations of Glucose/O2 Microfluidic Biofuel CellStack”Escalona-Villalpando, R.A., Hasan, K., Milton, R.D., Morena-Zuria, A., Arriaga, L.G., Minteer, S.D. and Ledesma-Garcia, J.* (2019).Journal of Power Sources, 414:150-157 “Methanococcus maripaludis Employs Three Functional Heterodisulfide Reductase Complexes forFlavin-Based Electron Bifurcation Using Hydrogen and Formate”Milton, R.D., Ruth, J.C., Deutzmann, J.S. and Spormann, A.M.* (2018).Biochemistry, 57(32):4848-4857 “Improved Performance of a Paper-Based Glucose Fuel Cell by Capillary Induced Flow”Del Torno-de Roman, L., Navarro, M., Hughes, G., Esquivel, J.P., Milton, R.D., Minteer, S.D. and Sabate, N.* (2018).Electrochimica Acta, 282:336-342 “Catalysts for Nitrogen Reduction to Ammonia”Foster, S.L., Perez Bakovic, S.I., Duda, R., Maheshwari, S., Milton, R.D., Minteer, S.D.*, Janik, M.J.*, Renner, J.N.* and Greenlee, L.F.* (2018).Nature Catalysis, 1:490-500 “Creating a Low-Potential Redox Polymer for Efficient Electroenzymatic CO2 Reduction”Yuan, M., Sahin, S., Cai, R., Abdellaoui, S., Hickey, D.P., Minteer, S.D. and Milton, R.D.* (2018).Angewandte Chemie International Edition, 57(22):6582-6586