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华修德,男,汉族,1984年3月生。主要从事农药残留与环境毒理研究。主持国家自然科学基金等项目10余项,以第一或通讯作者在Anal.Chem.、Biosens. Bioelectron.、ACS Appl.Mater.Inter.、J. Hazard. Mater.、Sens. Actuator B-Chem.、Anal. Chim. Acta.、Food Chem.、Microchim.Acta、Food Control等期刊上发表SCI论文30余篇,授权中国发明专利10余项,参与制定地方及团体标准10余项。获江苏省科学技术奖一等奖1项(第二完成人)、神农中华农业科技奖一等奖1项(第七完成人)、河南省科学技术进步奖二等奖1项(第四完成人)、中国植物保护学会科学技术奖一等奖1项(第二完成人)。现任南京农业大学植物保护学院农药科学系主任;入选江苏省高校“青蓝工程”优秀青年骨干教师,南京农业大学钟山学术新秀。担任中国植物保护学会青年委员会委员、中国植物保护学会农药残留与环境安全专业委员会;担任Biosensors期刊客座主编及Anal.Chem.;Biosens. Bioelectro.; Anal. Chim. Acta; J. Agric. Food Chem.; Food Chem.等期刊的审稿人。 教育经历: 2018/03–2019/03,美国加州大学戴维斯分校,农业与环境科学学院,访问学者 2007/09–2012/12,南京农业大学,植物保护学院,博士 2012/02–2012/08,美国加州大学戴维斯分校,农业与环境科学学院,访问学者 2002/09–2006/06,天津农学院,园艺系,学士 工作经历: 2020/01-至今,南京农业大学,植物保护学院,教授 2015/01-2019/12,南京农业大学,植物保护学院,副教授 2013/02–2014/12,南京农业大学,植物保护学院,讲师 主持或参加科研项目及人才计划项目情况: 1.国家自然科学基金面上项目,32172451,纳米抗体结构对互作多肽竞争抗原研制的影响及潜在机制探究,2022/01-2025/12,58万元,主持 2.国家自然科学基金面上项目,31972311,有机磷类农药噬菌体展示多肽模拟表位在淘选程序中的遗漏机制研究,2020/01-2023/12,58万元,主持 3.国家自然科学基金面上项目,31772194,纳米多肽竞争物与抗体互作的亲和力调控机制研究,2018/01-2021/12,65万元,主持 4.国家重点研发计划子课题,2016YFD0200207,化学农药在我国不同种植体系的归趋特征与限量标准,2016/01-2020/12,205万元,主持 5.国家重点研发计划子课题,2017YFF0210200,跨境生物产制品和工程生物检验检测及控制技术研究,2017/07-2020/12,50万元,主持 6.成果技术转移,基于光学三原色的多色测流免疫层析试纸条及其制备和检测方法,2021/12-2024/11,100万元,主持 7.技术开发/服务,二甲戊灵和多菌灵免疫检测试剂的研制,2021/8-2022/7,10万元,主持 8.技术开发/服务,冷杉醇单克隆抗体和细胞株的研制,2021/5-2022/12,10万元,主持 9.技术开发/服务,莨菪亭单克隆抗体和细胞株的研制,2021/7-2022/12,10万元,主持 10.技术开发/服务,二氯喹啉酸、苄嘧磺隆胶体金免疫层析试纸条研制,2021,10万元,主持 11.南京农业大学基本科研业务费专项基金项目,噬菌体展示多肽竞争物的纳米化及活性机理研究,2016/4-2019/3,10万元,主持 12.国家自然科学基金青年项目,31301690,氯噻啉重链抗体亲和性和特异性关键氨基酸残基作用机理的研究,2014/01-2016/12,25万元,主持 13.教育部高等学校博士点基金课题,20130097120006,氯噻啉重链抗体的体外进化及关键氨基酸残基功能的研究,2014/01-2016/12,4万元,主持 授权发明专利 1.华修德,王鸣华,丁园,陈贺,孙娜娜,一种氯噻啉的双信号侧流免疫层析检测方法,ZL201910965914.4 2.华修德,王鸣华,陈贺,丁园,基于光学三原色的多色测流免疫层析试纸条及其制备和检测方法,ZL202110170874.1 3.华修德,王鸣华,周亮亮,丁园,苯噻菌酯免疫复合体特异性结合的多肽及其用途,ZL201510418524.7。 4.华修德,王鸣华,刘凤权,施海燕,噬菌体免疫环介导等温扩增检测法,ZL201410272493.4。 5.王鸣华,华修德,冯璐,施海燕,苯噻菌酯抗体特异性结合的多肽及其用途,ZL201510418522.8 6.王鸣华,华修德,殷维,施海燕,一种抗噻虫啉单克隆抗体及其用途,ZL201510174884.7。 7.王鸣华,赵百萍,华修德,一株可有效降解炔苯酰草胺的降解菌及其应用,ZL201410017230.9。 8.陆艺文,王鸣华,夏亚中,华修德,周秋君,李伟,抗联苯菊酯单克隆抗体及其用途,201410359926X。 9.王鸣华,袁玉龙,华修德,施海燕,苯噻菌酯半抗原、人工抗原、特异抗体制备方法及其用途,ZL201310667544.9。 10.刘凤权,王利民,华修德,李刚,一种同时检测吡虫啉和甲基对硫磷的双特异单克隆抗体,ZL201210238321.6。 11.刘凤权,王利民,华修德,有机磷农药多残留快速免疫金标试纸条及其制备方法,ZL201210237844.9。 12.刘凤权,王利民,秦娜,方庆奎,华修德,抗三种有机磷农药的单克隆抗体及其应用,ZL201310365944.4。 13.刘凤权,王利民,方庆奎,华修德,抗氨基甲酸肟酯类农药丁酮威单克隆抗体的制备方法,ZL201310134272.6。 奖励 华修德(2/11),农产品中农药残留快速免疫检测技术与产品,江苏省科学技术奖,一等奖,2017(2017-1-24-R2) 华修德(7/12),农产品中农药与重金属快速免疫检测技术创建及应用,神农中华农业科技奖,一等奖,2017(KJ2017-D1-025-07) 华修德(4/10),草莓农药安全合理使用关键控制技术体系创建及应用,河南省科学技术进步奖,二等奖,2021(2021-J-034-R04/10) 华修德(2/11),农产品与环境中农药、重金属快速检测技术与产品,中国植物保护学会,科学技术奖,一等奖,2016(2016-YJ-1-02-R02) 教学农药登记与管理、农药残留与农产品安全、农药加工与管理、植保研究方法、植物保护学前沿等。

研究领域

农药残留免疫快速检测试剂的研制及应用基础研究,包括农药抗原、单克隆抗体、纳米抗体、模拟表位、抗免疫复合体等免疫检测核心试剂的研制,新颖农药残留免疫检测技术的开发。

近期论文

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1.Jiao Li, Yuan Ding, He Chen, Wanlin Sun, Yue Huang, Fengquan Liu, Minghua Wang, Xiude Hua*. Development of an indirect competitive enzyme-linked immunosorbent assay for propiconazole based on monoclonal antibody. Food Control 2022, 134: 108751.2.Yuan Ding, He Chen, Jiao Li, Lianrun Huang, Guangyue Song, Zhenfeng Li, Xiude Hua*, Gualberto Gonzalez-Sapienza, Bruce D. Hammock, Minghua Wang. Sortase- Mediated Phage Decoration for Analytical Applications. Analytical Chemistry 2021, 93:11800−11808.3.He Chen, Tianyang You, Lingfeng Zong, Liliya I. Mukhametova, Dmitry O. Zherdev, Sergei A. Eremin, Yuan Ding, Minghua Wang, Xiude Hua*. Competitive and noncompetitive fluorescence polarization immunoassays for the detection of benzothiostrobinusing FITC-labeled dendrimer-like peptides. Food Chemistry 2021, 360: 130020.4.He Chen, Wanlin Sun, Zhongrong Zhang, Zhexuan Tao, Yuling Qin, Yuan Ding, Limin Wang, Minghua Wang, Xiude Hua*. Competitive immune‑nanoplatforms with positive readout for the rapid detection of imidacloprid using gold nanoparticles. Microchimica Acta 2021, 188: 356.5.Mei Du, Qian Yang, Weimei Liu, Yuan Ding, He Chen, Xiude Hua*, Minghua Wang. Development of immunoassays with high sensitivity for detecting imidacloprid in environment and agro-products using phage-borne peptides. Science of the Total Environment 2020, 723: 137909.6.He Chen, Yuan Ding, Qian Yang, Bogdan Barnych, Gualberto González-Sapienza, Bruce D. Hammock, Minghua Wang, Xiude Hua*. Fluorescent “Turn off-on” Small-Molecule-Monitoring Nanoplatform Based on Dendrimer-like Peptides as Competitors. ACS Applied Materials & Interfaces 2019, 11: 33380–33389.7.Yuan Ding, Xiude Hua*, He Chen, Gualberto Gonzalez-Sapienza, Bogdan Barnych, Fengquan Liu, Minghua Wang, Bruce D. Hammock. A dual signal immunochromatographic strip for the detection of imidaclothiz using a recombinant fluorescent-peptide tracer and gold nanoparticles. Sensors & Actuators: B. Chemical 2019, 297: 126714.8.He Chen, Qian Yang, Yuan Ding, Natalia Vasylieva, Candace S. Bever, Xiude Hua*, Minghua Wang, Bruce D. Hammock. Competitive and noncompetitive immunoassays for the detection of benzothiostrobin using magnetic nanoparticles and fluorescein isothiocyanate-labeled peptides. Analytical and Bioanalytical Chemistry 2019, 411: 527–535. 9.Yuan Ding, Xiude Hua*, Mei Du, Qian Yang, Lina Hou, Limin Wang, Fengquan Liu, Gualberto Gonzalez-Sapienza, Minghua Wang. Recombinant, Fluorescent, Peptidomimetic Tracer for Immunodetection of Imidaclothiz. Analytical Chemistry 2018, 90: 13996–14002. 10.Yuan Ding, Xiude Hua*, He Chen, Fengquan Liu, Gualberto González-Sapien, Minghua Wang. Recombinant peptidomimetic-nano luciferase tracers for sensitive single-step immunodetection of small molecules. Analytical Chemistry 2018, 90: 2230–2237.11.Xiude Hua, Yuan Ding, Jiachuan Yang, Ming Ma, Haiyan Shi, Minghua Wang*. Direct competitive fluoroimmunoassays for detection of imidaclothiz in environmental and agricultural samples using quantum dots and europium as labels. Science of the Total Environment 2017, 583: 222–227.12.Hongjie You1,Xiude Hua1,*, Lu Feng, Nana Sun, Qi Rui, Limin Wang,Minghua Wang. Competitive immunoassay for imidaclothiz using upconversion nanoparticles and gold nanoparticles as labels. Microchim Acta 2017, 184: 1085–1092. (co-first author)13.Yuan Ding, Xiude Hua*, Nana Sun, Jiachuan Yang, Jiaqi Deng, Haiyan Shi, Minghua Wang. Development of a phage chemiluminescent enzyme immunoassay with high sensitivity for the determination of imidaclothiz in agricultural and environmental samples. Science of the Total Environment 2017, 609: 854–860.14.Xiude Hua, Hongjie You, Peiwen Luo, Zhexuan Tao, He Chen, Fengquan Liu, Minghua Wang. Upconversion fluorescence immunoassay for imidaclothiz by magnetic nanoparticle separation. Analytical and Bioanalytical Chemistry 2017, 409: 6885–6892.15.Xiude Hua, Liangliang Zhou, Lu Feng, Yuan Ding, Haiyan Shi, Limin Wang, Shirley J. Gee, Bruce D. Hammock, Minghua Wang. Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin. Analytica Chimica Acta 2015, 890: 150–156.16.Qingkui Fang1, Limin Wang1, Xiude Hua1, Yulong Wang, Suyan Wang, Qi Cheng, Jia Cai, Fengquan Liu. An enzyme-linked chemiluminescent immunoassay developed for detection of Butocarboxim from agricultural products based on monoclonal antibody. Food Chemistry 2015, 166: 372–379. (co-first author)17.Xiude Hua, Xiaofeng Liu, Wei Yin, Yazhong Xia, Qiujun Zhou,Yiwen Lu,Wei Li, Haiyan Shi, Fengquan Liu, Minghua Wang. A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier. Science of the Total Environment 2015, 502: 246–251.18.Baiping Zhao1, Xiude Hua1, Fei Wang1, Weiliang Dong, Zhoukun Li, Yu Yang, Zhongli Cui, Minghua Wang. Biodegradation of propyzamide byComamonas testosteroneW1 and cloning of the propyzamide hydrolase genecamH. Bioresource Technology 2015, 179: 144–149. (co-first author)19.Xiude Hua, Wei Yin, Haiyan Shi, Ming Li, Yanru Wang, Hong Wang, Yonghao Ye, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock. Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agroproducts. Analytical Chemistry 2014, 86: 8441−8447.20.Xiude Hua, Guoliang Qian, Jifei Yang, Baishi Hu, Jiaqin Fan, Na Qin, Gang Li, Yuyan Wang, Fengquan Liu. Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples. Biosensors and Bioelectronics 2010, 26: 189–194.

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