Smirnoff, Nicholas - University of Exeter - Disciplines of Biosciences

个人简介

1981: PhD in Physiological Ecology, University of St Andrews 1978: BSc Botany, University of Bristol Career 2006-present: Professor of Plant Biochemistry, School of Biosciences, University of Exeter 2001-2006: Reader in Plant Biochemistry, School of Biosciences, University of Exeter 1998-2001: Senior Lecturer, Department of Biological Sciences, University of Exeter 1984-1998: Lecturer, Department of Biological Sciences, University of Exeter 1982-1984: Birkbeck College, University of London 1984: Agricultural and Food Research Council Wain Fellow

研究领域

My long term research interest is in the metabolism and functions of vitamin C (ascorbic acid) in plants. My laboratory has discovered the biosynthetic pathway of ascorbate and more recently we are focussing on control of synthesis in relation to light and its role in photoprotection of photosynthesis. I am more generally interested in the roles of antioxidants and reactive oxygen species. Biochemical and molecular genetics approaches are used, focussing on Arabidopsis thaliana but with increasing emphasis on algae cyanobacteria. I am director of the Exeter Mass Spectrometry Facility.My current research focuses on the following areas. The functions of reactive oxygen species (ROS) and antioxidants in plants. Currently, the focus is on the metabolism and functions of ascorbate (vitamin C) and imaging hydrogen peroxide production. Plant and algal metabolism and metabolomics. We are developing LC-MS and GC-MS metabolite profiling methods and mass spectral databases for plants and algae. Metabolism of the coccolithophore alga Emiliana huxleyii and the function of calcification. Expression and characterization of synthetic photosynthetic metabolons in cyanobacteria. Imaging plant chemical composition and metabolism using Raman microscopy. Metabolic engineering. As part of the Exeter Microbial Biofuels Group, I provide expertise in analytical biochemistry and metabolism to engineering hydrocarbon biosynthesis.

近期论文

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Howard TP, Middelhaufe S, Moore K, Edner C, Kolak DM, Taylor GN, Parker DA, Lee R, Smirnoff N, Aves SJ, et al (In Press). Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli. PNAS Abstract. Author URL. Singleton C, Howard TP, Smirnoff N (In Press). Synthetic metabolons for metabolic engineering. Journal of Experimental Botany Abstract. Full text. Page M, Sultana N, Paszkiewicz K, Florance H, Smirnoff N (In Press). The influence of ascorbate on anthocyanin accumulation during high light acclimation in Arabidopsis thaliana: further evidence for redox control of anthocyanin synthesis. Plant, Cell & Environment, 35, 388-404. Abstract. Full text. Lim B, Smirnoff N, Cobbett CS, Goltz JF (2016). Ascorbate-Deficient vtc2 Mutants in Arabidopsis Do Not Exhibit Decreased Growth. Frontiers in Plant Science, 7, 1025-1025. Full text. de Torres Zabala M, Zhai B, Jayaraman S, Eleftheriadou G, Winsbury R, Yang R, Truman W, Tang S, Smirnoff N, Grant M, et al (2016). Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection. New Phytologist, 209(3), 1120-1134. Abstract. Full text. de Torres Zabala M, Zhai B, Jayaraman S, Eleftheriadou G, Winsbury R, Yang R, Truman W, Tang S, Smirnoff N, Grant M, et al (2016). Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection. New Phytol, 209(3), 1120-1134. Abstract. Author URL. Bechtold U, Penfold CA, Jenkins DJ, Legaie R, Moore JD, Lawson T, Matthews JS, Vialet-Chabrand SR, Baxter L, Subramaniam S, et al (2016). Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis. Plant Cell, 28(2), 345-366. Abstract. Author URL. Full text. Goodey NA, Florance HV, Smirnoff N, Hodgson DJ (2015). Aphids Pick Their Poison: Selective Sequestration of Plant Chemicals Affects Host Plant Use in a Specialist Herbivore. J Chem Ecol, 41(10), 956-964. Abstract. Author URL. Sultana N, Florance HV, Johns A, Smirnoff N (2015). Ascorbate deficiency influences the leaf cell wall glycoproteome in Arabidopsis thaliana. Plant, Cell and Environment, 38(2), 375-384. Abstract. Sultana N, Florance HV, Johns A, Smirnoff N (2015). Ascorbate deficiency influences the leaf cell wall glycoproteome in Arabidopsis thaliana. Plant Cell Environ, 38(2), 375-384. Abstract. Author URL. Full text. de Torres M, Littlejohn G, Jayaraman S, Studholme D, Bailey TC, Lawson T, Delfino L, Licht D, Truman W, Bölter B, et al (2015). Chloroplasts play a central role in plant defence and are targeted by pathogen effectors. Nature Plants, 1 Full text. Wheeler GL, Smirnoff N, Ishikawa T (2015). EVOLUTION OF ALTERNATIVE PATHWAYS FOR VITAMIN C SYNTHESIS FOLLOWING PLASTID ACQUISITION. EUROPEAN JOURNAL OF PHYCOLOGY, 50, 52-52. Author URL. Wheeler G, Ishikawa T, Pornsaksit V, Smirnoff N (2015). Evolution of alternative biosynthetic pathways for vitamin C following plastid acquisition in photosynthetic eukaryotes. Elife, 4 Abstract. Author URL. Full text. Littlejohn GR, Mansfield JC, Parker D, Lind R, Perfect S, Seymour M, Smirnoff N, Love J, Moger J (2015). In vivo chemical and structural analysis of plant cuticular waxes using stimulated Raman scattering microscopy. Plant Physiol, 168(1), 18-28. Abstract. Author URL. Full text. Proctor MCF, Smirnoff N (2015). Photoprotection in bryophytes: Rate and extent of dark relaxation of non-photochemical quenching of chlorophyll fluorescence. Journal of Bryology, 37(3), 171-177. Abstract. MacGregor DR, Kendall SL, Florance H, Fedi F, Moore K, Paszkiewicz K, Smirnoff N, Penfield S (2015). Seed production temperature regulation of primary dormancy occurs through control of seed coat phenylpropanoid metabolism. New Phytol, 205(2), 642-652. Abstract. Author URL. Lewis LA, Polanski K, de Torres-Zabala M, Jayaraman S, Bowden L, Moore J, Penfold CA, Jenkins DJ, Hill C, Baxter L, et al (2015). Transcriptional Dynamics Driving MAMP-Triggered Immunity and Pathogen Effector-Mediated Immunosuppression in Arabidopsis Leaves Following Infection with Pseudomonas syringae pv tomato DC3000. Plant Cell, 27(11), 3038-3064. Abstract. Author URL. Littlejohn GR, Mansfield JC, Christmas JT, Witterick E, Fricker MD, Grant MR, Smirnoff N, Everson RM, Moger J, Love J, et al (2014). An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology. Front Plant Sci, 5 Abstract. Author URL. Full text. Cresswell JE, Robert FXL, Florance H, Smirnoff N (2014). Clearance of ingested neonicotinoid pesticide (imidacloprid) in honey bees (Apis mellifera) and bumblebees (Bombus terrestris). Pest Management Science, 70(2), 332-337. Abstract. Full text. Cresswell JE, Robert FX, Florance H, Smirnoff N (2014). Clearance of ingested neonicotinoid pesticide (imidacloprid) in honey bees (Apis mellifera) and bumblebees (Bombus terrestris). Pest Manag Sci, 70(2), 332-337. Abstract. Author URL.