Mullineaux, Philip M - University of Essex - School of Biological Sciences

个人简介

1978 BSc(Hons; Class 1) University of Wales (Swansea) 1981 PhD University of Wales (Swansea) Thesis title: The relationship between photosynthesis and nitrogen fixation in the cyanobacterium Gloeocapsa (Gloeothece) CCAP sp 1430/3. 1981-1983 MRC Research Fellow, University of Edinburgh 1983-1986 Agrigenetics-Funded SSO at John Innes Institute 1986-1989 Complemented SSO (BBSRC) post John Innes Institute 1989-2000 Complemented post: Project Leader (band 4) John Innes Centre 2000-2004 Associate Head of Dept. of Disease and Stress Biology, John Innes Centre 1st June 2004-present. Professor of Plant Molecular Biology, University of Essex. 1st August 2008 - 31st July 2011 Direct of Research, School of Biological Sciences, University of Essex

研究领域

Signalling pathways that control defence gene expression in plants subject to high light stress Redox-mediated coordination of abiotic and biotic stress defence signalling pathways with special reference to heat shock transcription factors Drought responsive gene expression in legumes and Arabidopsis thaliana (thale cress) Identification of novel genes and processes from C3 desert plants that can be exploited for biotechnological applications Systems biology led modelling of high light responses and interaction with the regualtion of basla immunity in Arabidopsis Development of genetic probes based on GFP for non-invasive detection and quantification of hydrogen peroxide accumulation in subcellular compartments As a consequence of living in a constantly changing environment, from which they cannot move, plants have to have sophisticated means of monitoring and responding to changes in their environment. A major aspect of my research is focused on defining the signalling pathways that initiate these responses. An important consequence of such studies is to understand how signalling translates into altered physiological functions of the plants such that they become acclimated to their new conditions. One particular challenge is to translate what we learn in the laboratory to the response of the plant in the field or in its natural habitat. Particularly pertinent are questions about the functioning of signalling networks when the plant is challenged with multiple and fluctuating changes in the environment. In order to begin to understand this we are beginning to work with experimental systems that challenge the plant sequentially or simultaneously with more than one stress. Analysis of the genes that are altered in their expression and the signalling pathways that regulate them are then compared with plants challenged conventionally with a single stress. The final challenge is to move or research from the laboratory to the field Our initial studies have concentrated on the response of Arabidopsis thaliana (thale cress) to a sudden increase in light intensity. This is a continual problem that plants face, and as a consequence they often absorb more light energy than they can use for photosynthesis. To get around this problem, plants dissipate excess light energy (known as excitation energy) using a variety of processes. Some of these processes (such as the water-water cycle and photorespiration) generate reactive oxygen species (ROS) which are removed by a network of lower molecular weight antioxidants (eg. glutathione and ascorbate) and enzymes (such as superoxide dismutases and ascorbate peroxidases). If the production of ROS exceeds the capacity of the antioxidant network to deal with them, then cells suffer oxidative damage, which is often manifested as bleaching, paling or bronzing of leaves. Therefore a key part of how plants acclimate to such conditions is to adjust the functioning of their antioxidant network to suit their situation. The signal for making these adjustments is the ROS themselves and separate signalling routes controlled by the thiol antioxidant glutathione. An important part of acclimation to a changed environment may include the setting of thresholds for when such defences are deployed. The Mullineaux laboratory moved from the John Innes Centre to the University of Essex on 1st June 2004 and this has provided additional opportunities to initiate research into the genes underlying and influencing water productivity, i.e. the amount of lifetime water consumed as a function of its harvestable product. This work has led to patent filing on the role of a transcription factor in this response. Funding by BBSRC in the SABR initiative has allows us, in collaboration with the Universities of Exeter and Warwick, to begin to develop a Systems Biology approach leading to a holistic view of how signalling pathways integrate and are coordinated to elicit a response at the whole plant level to high light.

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Vickers CE, Possell, M., Cojocariu, CI, Velikova VB, Laothawornkitkul, J, Ryan A, Mullineaux PM and Hewitt CN (2009). Isoprene synthesis protects transgenic tobacco plants from oxidative stress. Plant Cell Environ. 32: 520-531. Chang C.C.C., Slesak I., Jorda L., Sonikov A., Melzer M, Miszalski Z, Mullineaux PM, Parker JE, Karpinska B and Karpinski S (2009) Arabidopsis chloroplastic glutathione peroxidases play a role in cross talk between photooxidative stress and immune responses. Plant Physiology 150: 670-683. Bechtold U, Rabbani N, Mullineaux PM, Thornalley PJ (2009) Quantitative measurement of specific biomarkers for protein oxidation, nitration and glycation in Arabidopsis leaves. Plant J. 59: 661-671. Galvez-Valdivieso G, Fryer MJ, Lawson T, Slattery K, Truman W, Smirnoff N, Asami T, Davies WJ, Jones AM, Baker NR and Mullineaux PM (2009) The high light response in Arabidopsis involves ABA signaling between vascular and bundle sheath cells. Plant Cell 21: 2143-2162 Galvez-Valdivieso G and Mullineaux PM (2010) The role of reactive oxygen species in signallingfrom chloroplasts to the nucleus. Physiologia Plantarum 138: 430–439. Possell M., Ryan A., Vickers CE, Mullineaux PM and Hewitt CN (2010) Effects of fosmidomycin on plant photosynthesis as measured by gas exchange and chlorophyll fluorescence. Photosynth. Res. 104: 49-59. Vickers CE, Possell M, Hewitt CN and Mullineaux PM (2010) Genetic structure and regulation of isoprene synthase in Poplar (Populus spp.). Plant Mol Biol 73: 547-558. Escobar C, Garcia A, Aristizabal F, Portillo M, Herreros E, Munoz-Martin MA, Grundler F, Mullineaux PM and Fenoll C (2010) Activation of geminivirus V-sense promoters in roots is restricted to nematode feeding sites. Mol Plant Pathology 11: 409-417. Mullineaux PM and Baker NR (2010) Oxidative stress: Antagonistic signaling for acclimation or cell death? Plant Physiology 154: 521-525. Bechtold U, Lawson T, Mejia-Carranza J, Meyer RC, Brown IR, Altmann T, Ton J and Mullineaux PM (2010) Constitutive salicylic acid defences do not compromise seed yield, drought tolerance and water productivity in the Arabidopsis accession C24. Plant Cell Environ. 33: 1959-1973. Miozzi L, Catoni M, Fiorilli V, Mullineaux PM, Accotto GP and Lanfranco L (2011) Arbuscular mycorrhizal symbiosis limits foliar transcription responses to viral infection and favors long-term virus accumulation. Mol Plant Microbe Interactions 24: 1562-1572. Carvalho LC, Vilela BJ, Mullineaux PM and Amancio S (2011) Comparative transcriptomic profiling of Vitis vinifera under high light using a custom-made array and the Affymetrix GeneChip. Molecular Plant 4: 1038-1051. Vickers CE, Possell M, Laothawornkitkul J, Ryan AC, Hewitt CN and Mullineaux PM (2011) Isoprene synthesis in plants: lessons from a transgenic tobacco model. Plant Cell Environ.34: 1043-1053