Wu, Ronghu - Georgia Institute of Technology

个人简介

2019, GT College of Sciences Cullen-Peck Faculty Scholar Award 2016, ASMS Research Award 2015, NSF CAREER Award 2014, Blanchard Assistant Professorship 2002-2004, Alexander von Humboldt Research Fellowship in Germany

研究领域

Mass spectrometry (MS) plays an increasingly important role in biomedical research as an extremely powerful technique used to characterize proteins and protein post-translational modifications (PTMs), measure corresponding abundance changes and investigate protein interactions. Our research interests are primarily directed towards developing novel MS-based proteomics methods and applying them to the biomedical studies. LC-MS-based method development to study protein PTMs. Protein PTMs have a profound impact on protein structure, function and stability. Effective separation and enrichment methods are crucial for studying the modified forms of proteins present in cells or tissues. Due to the structural differences between the more than 200 PTMs reported, there is no common method suitable for analyzing numerous PTMs. Distinctive structures and properties of each group allow us to design specific methods to study individual protein modifications, which will be applied to biomedical research. Protein function, cell signaling and cancer. Cells respond to a plethora of intra- and extra-cellular signals. Ineffective or improper signal transductions, which are often carried out by protein modifications, may lead to human diseases such as cancer. Proteins and their PTM changes at different cellular states may be comprehensively analyzed by quantitative MS-based proteomics methods, which can improve our understanding of protein function, cell signaling pathways, and molecular implications of drug treatment. Thus, it will be possible to discover novel disease biomarkers, identify new drug targets and contribute to the selection of better targeted agents on an individual basis, which will result in potential applications in personalized medicine. Cell metabolism. Cell metabolism is directly related to many diseases, including cancer cells which have notably abnormal metabolism. The metabolism network is very intricate and complicated; hence investigation at the whole proteome level may provide new insight. Furthermore, the changes in metabolites and interactions between enzymes and metabolites may also be monitored by MS. Our research attempts to understand how proteins regulate the generation of metabolites, and how metabolites affect metabolic pathways. We are striving to unveil new regulatory mechanisms of cell metabolism, and to advance our knowledge of abnormal metabolism of cancer cells. A further understanding of the mechanistic links between cellular metabolism and signal pathways may ultimately lead to new drug discovery and benefits for human health.

近期论文

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Suttipong Suttapitugsakul#, Ming Tong#, and Ronghu Wu*, Time-Resolved and Site-Specific Analysis of Surface Protein N-Glycosylation Revealing Distinct Responses of Monocytes and Macrophages to LPS Treatment, Angewandte Chemie International Edition, 2021. DOI: 10.1002/anie.202102692. Fangxu Sun#, Suttipong Suttapitugsakul# and Ronghu Wu*, Unraveling the Surface Glycoprotein Interaction Network by Integrating Chemical Crosslinking with MS-Based Proteomics, Chemical Science, 12: 2146-2155, 2021. DOI: 10.1039/D0SC06327D. Suttipong Suttapitugsakul#, Ming Tong#, Fangxu Sun#, and Ronghu Wu*, Enhancing Comprehensive Analysis of Secreted Glycoproteins from Cultured Cells without Serum Starvation, Analytical Chemistry, 93(4): 2694-2705, 2021. DOI: 10.1021/acs.analchem.0c05126. Senhan Xu#, Fangxu Sun#, Ming Tong#, and Ronghu Wu*, MS-based proteomics for global investigation of protein O-GlcNAcylation, Molecular Omics (Invited Review), 2021. DOI: 10.1039/D1MO00025J. Ming Tong, Johanna Smeekens, Haopeng Xiao, and Ronghu Wu*, Systematic Quantification of the Dynamics of Newly Synthesized Proteins Unveiling Their Degradation Pathways in Human Cells, Chemical Science, 11(13): 3557-3568, 2020. Senhan Xu, Fangxu Sun, and Ronghu Wu*, A Chemoenzymatic Method Based on Easily Accessible Enzymes for Profiling Protein O-GlcNAcylation, Analytical Chemistry, 92(14): 9807-9814, 2020. Ming Tong, Suttipong Suttapitugsakul, and Ronghu Wu*, An Effective Method for Accurate and Sensitive Quantification of Rapid Changes of Newly Synthesized Proteins, Analytical Chemistry, 92(14): 10048–10057, 2020. Suttipong Suttapitugsakul#, Fangxu Sun#, and Ronghu Wu*, Recent Advances in Glycoproteomic Analysis by Mass Spectrometry, Analytical Chemistry (Invited Review Article), 92(1): 267-291, 2020. Anna Socha, Di Yang, Alicja Bulsiewicz, Kelvin Yaprianto, Marian Kupculak, Chih-Chao Liang, Andreas Hadjicharalambous, Ronghu Wu, Steven P Gygi, Martin A Cohn*, WRNIP1 Is Recruited to DNA Interstrand Crosslinks and Promotes Repair, Cell Reports, 32(1): 107850, 2020. Rebecca Guth-Metzler, Marcus S. Bray, Suttipong Suttapitugsakul#, Claudia Montllor-Albalate, Jessica C. Bowman, Ronghu Wu, Amit R. Reddi, C. Denise Okafor, Jennifer B. Glass*, and Loren D. Williams*, Cutting in-line with iron: ribosomal function and non-oxidative RNA cleavage, Nucleic Acid Res., 48(15): 8663-8674, 2020. Rui Liu; Dong Li; Fangxu Sun#; Antonio Rampoldi; Joshua Maxwell; Ronghu Wu; Peter Fischbach; Sharon Castellino; Yuhong Du; Haian Fu; Anant Mandawat; Chunhui Xu*, Melphalan induces cardiotoxicity through oxidative stress in cardiomyocytes derived from human induced pluripotent stem cells, Stem Cell Research & Therapy, 11(1): 1-17, 2020 Rui Liu, Fangxu Sun#, Parvin Forghani, Lawrence Armand, Antonio Rampoldi, Dong Li, Ronghu Wu, Chunhui Xu*, Proteomic profiling reveals roles of stress response, Ca2+ transient dysregulation and novel signaling pathways in alcohol-induced cardiotoxicity, Alcoholism: Clinical and Experimental Research, 44(11): 2187-2199, 2020. Suttipong Suttapitugsakul, Lindsey D. Ulmer, Chendi Jiang, Fangxu Sun, and Ronghu Wu*, Surface Glycoproteomic Analysis Reveals that both Unique and Differential Expression of Surface Glycoproteins Determine the Cell Type, Analytical Chemistry, 91(10), 6934-6942, 2019. Fangxu Sun, Suttipong Suttapitugsakul, and Ronghu Wu*, Enzymatic tagging of glycoproteins on the cell surface for their global and site-specific analysis with mass spectrometry, Analytical Chemistry, 91(6): 4195-4203, 2019. Fangxu Sun, Suttipong Suttapitugsakul, Haopeng Xiao and Ronghu Wu*, Comprehensive analysis of protein glycation reveals its potential impacts on protein degradation and gene expression, Journal of the American Society for Mass Spectrometry, 30(12): 2480-2490, 2019. Haopeng Xiao, Fangxu Sun, Suttipong Suttapitugsakul, and Ronghu Wu*, Global and site-specific analysis of protein glycosylation in complex biological systems with mass spectrometry, Mass Spectrometry Reviews, 38(4-5), 356-379, 2019. Fangxu Sun and Ronghu Wu*, Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomics, A book Chapter in Methods in enzymology, 626, 223-247, 2019. Suttipong Suttapitugsakul, Haopeng Xiao, Ronghu Wu*, Identification and Quantification of Newly Synthesized Proteins Using Mass Spectrometry‐Based Chemical Proteomics. A book chapter in Mass Spectrometry-Based Chemical Proteomics (Edited by W. Andy Tao and Ying Zhang), John Wiley & Sons, 2019. David Lopez-Martinez, Marian Kupculak, Di Yang, Yasunaga Yoshikawa, Chih-Chao Liang, Ronghu Wu, Steven P. Gygi, Martin A. Cohn*, Phosphorylation of FANCD2 Inhibits the FANCD2/FANCI Complex and Suppresses the Fanconi Anemia Pathway in the Absence of DNA Damage, Cell Reports, 27, 2990–3005, 2019 Santi Mestre-Fos, Petar I. Penev, Suttipong Suttapitugsakul, Michael Hu, Chieri Ito, Anton S. Petrov, Roger M. Wartell, Ronghu Wu and Loren Dean Williams*, G-quadruplexes in human ribosomal RNA, Journal of Molecular Biology, 430 (10): 1940-1955, 2019. Alexis Zukowski, Juliana Phillips, Soyeon Park, Ronghu Wu, Stephen P. Gygi, and Aaron M. Johnson*, Proteomic Profiling of Yeast Heterochromatin Connects Direct Physical and Genetic Interactions, Current Genetics, 65 (2): 495-505, 2019.