Jones, Patrik R. - Imperial College London - Department of Life Sciences, Biochemistry and Biotechnology

研究领域

The objective of our research is to understand and engineer the metabolism of biotechnologically important microorganisms in order to transform solar to chemical energy. Towards understanding, we study the metabolism of model prokaryotes (E. coli, fermentative; cyanobacteria, autotrophic) using both computational and experimental methods. In engineering, we are particularly interested in the design, construction and evaluation of synthetic metabolic pathways. The current research can be illustrated and broadly categorized as follows: MME Group Research Overview 1) Synthetic metabolic pathways for industrial production of chemicals. We have an interest in creating solutions that consider all steps of the production chain, including separation and utility. The ideal metabolic pathway is not always available, and in that situation we seek to create synthetic routes for compounds without a known biological pathway, or chemicals with no known biogenic origin. A typical example of this work is the engineering of a synthetic pathway for biosynthesis of propane. In current projects we are also expanding these efforts beyond fuels and creating solutions that by-pass native host metabolic limitations. 2) Engineering cyanobacteria for chemicals production. Cyanobacteria can in theory be used as an easily amplified catalyst for the conversion of solar energy and carbon dioxide into chemicals. Currently, there is effectively no large-scale commercial production of chemicals using engineered cyanobacteria or algae. We would like to contribute towards the development and commercialisation of such technology, but work strictly in the early phase of proof-of-concept for strain development through genetic engineering. An example of this work is the construction of cyanobacteria strains that produce ethylene, incorporating molecular toolbox development work. 3) Understanding metabolism. In order to engineer metabolism it is first necessary to understand it. Our knowledge of cyanobacteria metabolism is in some cases quite limited. This work is mainly carried out on a systems level, including computational network analysis, genome-scale stoichiometric modelling and targeted or non-targeted proteomics. Most of this work is unpublished, an example is our joint work on text-mining with the Filip Ginter group at Univ. Turku.

近期论文

查看导师最新文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Vuorijoki L, Isojarvi J, Kallio P, Kouvonen P, Aro E-M, Corthals GL, Jones PR, Muth-Pawlak Det al., 2016, Development of a Quantitative SRM-Based Proteomics Method to Study Iron Metabolism of Synechocystis sp PCC 6803, JOURNAL OF PROTEOME RESEARCH, Vol: 15, Pages: 266-279, ISSN: 1535-3893 Zavrel T, Knoop H, Steuer R, Jones PR, Cerveny J, Trtilek Met al., 2016, A quantitative evaluation of ethylene production in the recombinant cyanobacterium Synechocystis sp PCC 6803 harboring the ethylene-forming enzyme by membrane inlet mass spectrometry, BIORESOURCE TECHNOLOGY, Vol: 202, Pages: 142-151, ISSN: 0960-8524 Akhtar MK, Dandapani H, Thiel K, Jones PRet al., 2015, Microbial production of 1-octanol: A naturally excreted biofuel with diesel-like properties., Metab Eng Commun, Vol: 2, Pages: 1-5 Kremer F, Blank LM, Jones PR, Akhtar MKet al., 2015, A Comparison of the Microbial Production and Combustion Characteristics of Three Alcohol Biofuels: Ethanol, 1-Butanol, and 1-Octanol., Front Bioeng Biotechnol, Vol: 3 Menon N, Pasztor A, Menon BRK, Kallio P, Fisher K, Akhtar MK, Leys D, Jones PR, Scrutton NSet al., 2015, A microbial platform for renewable propane synthesis based on a fermentative butanol pathway, Biotechnology for Biofuels, Vol: 8, ISSN: 1754-6834 Pasztor A, Kallio P, Malatinszky D, Akhtar MK, Jones PRet al., 2015, A Synthetic O-2-Tolerant Butanol Pathway Exploiting Native Fatty Acid Biosynthesis in Escherichia coli, BIOTECHNOLOGY AND BIOENGINEERING, Vol: 112, Pages: 120-128, ISSN: 0006-3592 Akhtar MK, Jones PR, 2014, Cofactor engineering for enhancing the flux of metabolic pathways., Front Bioeng Biotechnol, Vol: 2 Jones PR, 2014, Genetic instability in cyanobacteria – an elephant in the room?, Frontiers in Bioengineering and Biotechnology, Vol: 2, Pages: 1-5 Kallio P, Pasztor A, Thiel K, Akhtar MK, Jones PRet al., 2014, An engineered pathway for the biosynthesis of renewable propane, NATURE COMMUNICATIONS, Vol: 5, ISSN: 2041-1723 Kallio P, Pásztor A, Akhtar MK, Jones PRet al., 2014, Renewable jet fuel., Curr Opin Biotechnol, Vol: 26, Pages: 50-55 Akhtar MK, Turner NJ, Jones PR, 2013, Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 87-92, ISSN: 0027-8424 Kaewphan S, Kreula S, Van Landeghem S, Van de Peer Y, Jones PR, Ginter Fet al., 2013, Integrating Large-Scale Text Mining and Co-Expression Networks: Targeting NADP (H) Metabolism in E. coli with Event Extraction, BioTxtM 2012 Third workshop on Building and Evaluating Resources for Biomedical Text Mining, Pages: 8-15 Guerrero F, Carbonell V, Cossu M, Correddu D, Jones PRet al., 2012, Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp PCC 6803, PLOS ONE, Vol: 7, ISSN: 1932-6203 Kamarainen J, Knoop H, Stanford NJ, Guerrero F, Akhtar MK, Aro E-M, Steuer R, Jones PRet al., 2012, Physiological tolerance and stoichiometric potential of cyanobacteria for hydrocarbon fuel production, JOURNAL OF BIOTECHNOLOGY, Vol: 162, Pages: 67-74, ISSN: 0168-1656 Eser BE, Das D, Han J, Jones PR, Marsh ENGet al., 2011, Oxygen-Independent Alkane Formation by Non-Heme Iron-Dependent Cyanobacterial Aldehyde Decarbonylase: Investigation of Kinetics and Requirement for an External Electron Donor, BIOCHEMISTRY, Vol: 50, Pages: 10743-10750, ISSN: 0006-2960 Akhtar MK, Jones PR, 2009, Construction of a synthetic YdbK-dependent pyruvate:H-2 pathway in Escherichia coli BL21(DE3), METABOLIC ENGINEERING, Vol: 11, Pages: 139-147, ISSN: 1096-7176 Akhtar MK, Jones PR, 2008, Deletion of iscR stimulates recombinant clostridial Fe-Fe hydrogenase activity and H-2-accumulation in Escherichia coli BL21(DE3), APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, Vol: 78, Pages: 853-862, ISSN: 0175-7598 Akhtar MK, Jones PR, 2008, Engineering of a synthetic hydF-hydE-hydG-hydA operon for biohydrogen production, ANALYTICAL BIOCHEMISTRY, Vol: 373, Pages: 170-172, ISSN: 0003-2697 Jones PR, 2008, Improving fermentative biomass-derived H-2-production by engineering microbial metabolism, 2nd Asian Bio-Hydrogen Symposium, Publisher: PERGAMON-ELSEVIER SCIENCE LTD, Pages: 5122-5130, ISSN: 0360-3199 Jones PR, Gawel R, Francis IL, Waters EJet al., 2008, The influence of interactions between major white wine components on the aroma, flavour and texture of model white wine, Food Quality and Preference, Vol: 19, Pages: 596-607, ISSN: 0950-3293