Heap, John - Imperial College London - Department of Life Sciences, Biochemistry and Biotechnology

研究领域

Dr John Heap is a Lecturer in Synthetic Biology in the Department of Life Sciences. His research group is based in the Centre for Synthetic Biology and Innovation (CSynBI). We are interested in the design and construction of synthetic biological systems which process matter, energy and information in useful ways not observed in nature. Our research involves both the development of foundational biological technologies and their use for applications, mainly in chemical manufacture and in health. An important aspect of this research is developing reliable approaches to implement synthetic biological systems using diverse organisms important (or with the potential to be) in industrial or medical applications. Engineering metabolism is often important for our applications, and a particular interest is how non-natural metabolic constraints can be used to re-direct fluxes and achieve high yields. Our projects often benefit from multidisciplinary collaborations, such as our work on engineering bacteria (Clostridium sporogenes) to target and digest tumours, which we are pursuing in collaboration with mathematicians (Diego Oyarzún's lab).

近期论文

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Ehsaan M, Kuit W, Zhang Y, Cartman ST, Heap JT, Winzer K, Minton NPet al., 2016, Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824, Biotechnology for Biofuels, Vol: 9, Pages: 4-4, ISSN: 1754-6834 Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero J, Estévez A, Kato A, Marqvorsen MH, Fleet GW, Estévez RJ, Izumori K, Heap JTet al., 2016, Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the rhaBAD Promoter., ACS Synth Biol, Vol: 5, Pages: 1136-1145 Liu Z, Yoshihara A, Kelly C, Heap JT, Marqvorsen MHS, Jenkinson SF, Wormald MR, Otero JM, Estevez A, Kato A, Fleet GWJ, Estevez RJ, Izumori Ket al., 2016, 6-Deoxyhexoses from l-Rhamnose in the Search for Inducers of the Rhamnose Operon: Synergy of Chemistry and Biotechnology, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 22, Pages: 12557-12565, ISSN: 0947-6539 Walker DJ, Heap JT, Winzer K, Minton NPet al., 2016, A genetic assay for gene essentiality in Clostridium., Anaerobe, Vol: 42, Pages: 40-43 Cartman ST, Heap JT, Kuehne S, Cooksley CM, Ehsaan M, Winzer K, Minton NPet al., 2014, Clostridial gene tools, Systems Biology of Clostridium, Pages: 21-55, ISBN: 9781783264414 Heap JT, Theys J, Ehsaan M, Kubiak AM, Dubois L, Paesmans K, Mellaert LV, Knox R, Kuehne SA, Lambin P, Minton NPet al., 2014, Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo., Oncotarget, Vol: 5, Pages: 1761-1769 Jabbari S, Steiner E, Heap JT, Winzer K, Minton NP, King JRet al., 2013, The putative influence of the agr operon upon survival mechanisms used by Clostridium acetobutylicum, MATHEMATICAL BIOSCIENCES, Vol: 243, Pages: 223-239, ISSN: 0025-5564 Kovács K, Willson BJ, Schwarz K, Heap JT, Jackson A, Bolam DN, Winzer K, Minton NPet al., 2013, Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824., Biotechnol Biofuels, Vol: 6 Zhang Z, Korkeala H, Dahlsten E, Sahala E, Heap JT, Minton NP, Lindström Met al., 2013, Two-component signal transduction system CBO0787/CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502., PLoS Pathog, Vol: 9 Cartman ST, Kelly ML, Heeg D, Heap JT, Minton NPet al., 2012, Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production., Appl Environ Microbiol, Vol: 78, Pages: 4683-4690 Heap JT, Ehsaan M, Cooksley CM, Ng YK, Cartman ST, Winzer K, Minton NPet al., 2012, Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker., Nucleic Acids Res, Vol: 40 Lindström M, Dahlsten E, Söderholm H, Selby K, Somervuo P, Heap JT, Minton NP, Korkeala Het al., 2012, Involvement of two-component system CBO0366/CBO0365 in the cold shock response and growth of group I (proteolytic) Clostridium botulinum ATCC 3502 at low temperatures., Appl Environ Microbiol, Vol: 78, Pages: 5466-5470 Saad NY, Schiel B, Brayé M, Heap JT, Minton NP, Dürre P, Becker HDet al., 2012, Riboswitch (T-box)-mediated control of tRNA-dependent amidation in Clostridium acetobutylicum rationalizes gene and pathway redundancy for asparagine and asparaginyl-trnaasn synthesis., J Biol Chem, Vol: 287, Pages: 20382-20394 Jabbari S, Heap JT, King JR, 2011, Mathematical Modelling of the Sporulation-Initiation Network in Bacillus Subtilis Revealing the Dual Role of the Putative Quorum-Sensing Signal Molecule PhrA, BULLETIN OF MATHEMATICAL BIOLOGY, Vol: 73, Pages: 181-211, ISSN: 0092-8240 Selby K, Lindstrom M, Somervuo P, Heap JT, Minton NP, Korkeala Het al., 2011, Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol: 77, Pages: 2823-2830, ISSN: 0099-2240 Soderholm H, Lindstrom M, Somervuo P, Heap J, Minton N, Linden J, Korkeala Het al., 2011, cspB encodes a major cold shock protein in Clostridium botulinum ATCC 3502, INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, Vol: 146, Pages: 23-30, ISSN: 0168-1605 Steiner E, Dago AE, Young DI, Heap JT, Minton NP, Hoch JA, Young Met al., 2011, Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum, MOLECULAR MICROBIOLOGY, Vol: 80, Pages: 641-654, ISSN: 0950-382X Bradshaw M, Marshall KM, Heap JT, Tepp WH, Minton NP, Johnson EAet al., 2010, Construction of a Nontoxigenic Clostridium botulinum Strain for Food Challenge Studies, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol: 76, Pages: 387-393, ISSN: 0099-2240 Burns DA, Heap JT, Minton NP, 2010, SleC Is Essential for Germination of Clostridium difficile Spores in Nutrient-Rich Medium Supplemented with the Bile Salt Taurocholate, JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 657-664, ISSN: 0021-9193 Burns DA, Heap JT, Minton NP, 2010, Clostridium difficile spore germination: an update, RESEARCH IN MICROBIOLOGY, Vol: 161, Pages: 730-734, ISSN: 0923-2508