Psakis, Georgios - Huddersfield University - Department of Biological Sciences

个人简介

Following completion of my BSc in Biochemistry at the University of Leeds (1999), I worked as a research assistant in the group of Prof. P.J.F. Henderson (University of Leeds), focusing on the elucidation of structure/function relationship of bacterial membrane transporters. Inspired by the works in the field of membrane biochemistry, I started my PhD (2001) in the structural/functional characterisation of the GalP transporter from Escherichia coli. In the course of my doctorate studies, I had the pleasure to work with Prof. Stephen Baldwin (University of Leeds) and Prof. So Iwata (Imperial College London). After submission of my PhD thesis (2004) I moved to Philipps University Marburg to work for Prof. Lars-Oliver Essen and the European Membrane Protein Concortium (e-MEP) on the development of medium-high throughput approaches for the cloning, expression, purification and crystallisation of integral membrane proteins from the genome of Helicobacter pylori (causative agent of ulcers and gastric cancer). In 2007, I embarked on my second Post-Doc in Justus-Liebig University Giessen, working for Prof. Jon Hughes in the elucidation of structure/function dynamics of cyanobacterial phytochromes (light-regulated histidine kinases). From 2011 to 2013, I directed the laboratory of Prof. Lars-Oliver Essen in Philipps University and my work focused on the structural/functional characterisation of native and chemically engineered ion channels. I joined the department of Biological Sciences at the University of Huddersfield in 2013.

研究领域

The main focus of my research is to investigate the structural changes that define the gating-behaviour of outer-membrane channels and to unravel the ways these structural/functional changes can regulate signalling cascades. Understanding of these relationships will enable the development of artificial channels with desired functionalities/characteristics for the generation of a new array of in vivo and in vitro biological tools. Currently, I focus on a) the chemical/biological engineering of the outer membrane protein G (OmpG) from E. coli and b) the biochemical and structural characterisation of Arabidopsis thaliana voltage dependent anion channels (VDACs).

近期论文

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Mertins, B., Psakis, G. and Essen, L. (2014) ‘VDAC: the wizard of mitochondrial OM’ Biological Chemistry , 395 (12), pp. 1435-1442. ISSN 1431-6730 Grosse, W., Psakis, G., Mertins, B., Reiss, P., Windisch, D., Brademann, F., Bürck, J., Ulrich, A., Koert, U. and Essen, L. (2014) ‘Structure-Based Engineering of a Minimal Porin Reveals Loop-Independent Channel Closure’ Biochemistry , 53 (29), pp. 4826-4838. ISSN 0006-2960 Sineshchekov, V., Mailliet, J., Psakis, G., Feilke, K., Kopycki, J., Zeidler, M., Essen, L. and Hughes, J. (2014) ‘Tyrosine 263 in Cyanobacterial Phytochrome Cph1 Optimizes Photochemistry at the prelumi-R?lumi-R Step’ Photochemistry and Photobiology , 90 (4), pp. 786-795. ISSN 00318655 Song, C., Psakis, G., Kopycki, J., Lang, C., Matysik, J. and Hughes, J. (2014) ‘The D-ring, Not the A-ring, Rotates in Synechococcus OS-B' Phytochrome’ Journal of Biological Chemistry , 289 (5), pp. 2552-2562. ISSN 0021-9258 Mertins, B., Psakis, G., Grosse, W., Back, K., Salisowski, A., Reiss, P., Koert, U. and Essen, L. (2012) ‘Flexibility of the N-Terminal mVDAC1 Segment Controls the Channel’s Gating Behavior’ PLoS ONE , 7 (10), p. e47938. ISSN 1932-6203 Song, C., Psakis, G., Lang, C., Mailliet, J., Zaanen, J., Gaertner, W., Hughes, J. and Matysik, J. (2011) ‘On the collective nature of phytochrome photoactivation’ Biochemistry , 50 (51), pp. 10987-10989. ISSN 0006-2960 Mailliet, J., Psakis, G., Feilke, K., Sineshchekov, V., Essen, L. and Hughes, J. (2011) ‘Spectroscopy and a high-resolution crystal structure of Tyr263 mutants of cyanobacterial phytochrome Cph1’ Journal of Molecular Biology , 413 (1), pp. 115-127. ISSN 0022-2836 Psakis, G., Mailliet, J., Lang, C., Teufel, L., Essen, L. and Hughes, J. (2011) ‘Signaling kinetics of cyanobacterial phytochrome Cph1, a light regulated histidine kinase’ Biochemistry , 50 (28), pp. 6178-6188. ISSN 0006-2960 Song, C., Psakis, G., Lang, C., Mailliet, J., Gaertner, W., Hughes, J. and Matysik, J. (2011) ‘Two ground state isoforms and a chromophore D-ring photoflip triggering extensive intramolecular changes in a canonical phytochrome’ Proceedings of the National Academy of Sciences , 108 (10), pp. 3842-3847. ISSN 00278424 Roeben, M., Hahn, J., Klein, E., Lamparter, T., Psakis, G., Hughes, J. and Schmieder, P. (2010) ‘NMR Spectroscopic Investigation of Mobility and Hydrogen Bonding of the Chromophore in the Binding Pocket of Phytochrome Proteins’ Chemical Physics and Physical Chemistry , 11 (6), pp. 1248-1257. ISSN 1439-7641 Mailliet, J., Psakis, G., Schroeder, C., Kaltofen, S., Dürrwang, U., Hughes, J. and Essen, L. (2009) ‘Dwelling in the dark: procedures for the crystallography of phytochromes and other photochromic proteins’ Acta Crystallographica Section D: Biological Crystallography , 65 (11), pp. 1232-1235. ISSN 1399-0047 Psakis, G., Polaczek, J. and Essen, L. (2009) ‘AcrB et al.: Obstinate contaminants in a picogram scale. One more bottleneck in the membrane protein structure pipeline’ Journal of Structural Biology , 166 (1), pp. 107-111. ISSN 1047-8477 Psakis, G., Saidijam, M., Shibayama, K., Polaczek, J., Bettaney, K., Baldwin, J., Baldwin, S., Hope, R., Essen, L., Essenberg, R. and Henderson, P. (2008) ‘The sodium-dependent D-glucose transport protein of Helicobacter pylori’ Molecular microbiology , 71 (2), pp. 391-403. ISSN 1365-2958 Patching, S., Psakis, G., Baldwin, S., Baldwin, J., Henderson, P. and Middleton, D. (2008) ‘Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR’ Molecular Membrane Biology , 25 (6-7), pp. 474-484. ISSN 0968-7688 (print), 1464-5203 (electronic) Psakis, G., Sandra, N., Holz, C., Kress, D., Maestre-Reyna, M., Polaczek, J., Illing, G. and Essen, L. (2007) ‘Expression screening of integral membrane proteins from Helicobacter pylori 26695’ Protein Science , 16 (12), pp. 2667-2676. ISSN 0961-8368 Saidijam, M., Bettaney, K., Szakonyi, G., Psakis, G., Shibayama, K., Suzuki, S., Clough, J., Blessie, V., Abbu-Bakr, A., Baumberg, S., Meuller, J., Hoyle, C., Palmer, S., Butaye, P., Walravens, K., Patching, S., O'reilly, J., Rutherford, N., Bill, R., Roper, D., Phillips-Jones, M. and Henderson, P. (2005) ‘Active membrane transport and receptor proteins from bacteria’ Biochemical Society Transactions , 33 (Pt 4), pp. 867-872. ISSN 0300-5127 (print), 1470-8752 (electronic) Saidijam, M., Psakis, G., Clough, J., Meuller, J., Suzuki, S., Hoyle, C., Palmer, S., Morrison, S., Pos, M., Essenberg, R., Maiden, M., Abu-bakr, A., Baumberg, S., Neyfakh, A., Griffith, J., Stark, M., Ward, A., O'Reilly, J., Rutherford, N., Phillips-Jones, M. and Henderson, P. (2003) ‘Collection and characterisation of bacterial membrane proteins’ FEBS Letters , 555 (1), pp. 170-175. ISSN 0014-5793