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讲授课程《兽医药理学A》、《兽医药理学综合实验》、《动物毒理学》、《动物毒理学实验》 博士,华中农业大学教授,博士生导师。自工作以来,一直在科研教学第一线开展兽药残留与食品安全的基础理论及关键技术研究和高层次人才培养。在国家各级项目(课题)的资助下,破解了小分子化合物广谱单克隆抗体难于制备的难题,发明了一系列具有完全自主知识产权的小分子半抗原、免疫原和包被原;创制了40多种灵敏、高产、优质单克隆抗体,研发出基于这些广谱抗体的试剂盒和试纸条48种。培养硕士研究生30名,指导学生在第三届中国“互联网+”大学生创新创业大赛湖北省复赛中获得创意组金奖。先后获包括国家技术发明奖二等奖(排名第2)在内的9项国家及省部级科技奖励;发表SCI论文52篇,其中以第一作者和通讯作者在包括Food Chemistry (IF 4.529)、Journal of Agricultural and Food Chemistry (IF 3.154)等本专业领域的Top期刊在内的国际期刊上发表SCI论文22篇,被引164次,他引130次,h-指数17。获授权发明专利36件,制订国家标准1项,农业部备案产品4个,专利保藏单克隆细胞株39株。 教育经历 1996.09 - 2000.06, 湖北农学院,动物科学,农学学士 2000.09 - 2005.06, 华中农业大学,预防兽医学专业毕业,农学博士 工作经历 2005.07-2009.12,华中农业大学 讲师 2009.12-2018.11,华中农业大学 副教授,硕士生导师 2018.11-至今,华中农业大学 教授,博士生导师 专利类 1. 硝基咪唑类药物的单克隆抗体及酶联免疫方法与试剂盒。ZL201410830051.7。序2 2. 用于检测食品中头孢类抗生素残留的抗体芯片试剂盒及方法。ZL201410830203.3。序2 3. 用于检测食品中氨基糖苷类抗生素残留的抗体芯片试剂盒及方法。ZL201410829545.3。序2 4. 用于检测食品中磺胺类药物残留的抗体芯片试剂盒及方法。ZL201410830693.7。序2 5. 用于检测呋喃它酮残留标识物AMOZ的单抗及酶联免疫方法与试剂盒。ZL201210176537.4。序2 6. 一种单克隆抗体,包含该单克隆抗体的检测试剂盒及应用。ZL200510011521.8。序2 7. 用于检测卡巴氧和喹赛多代谢产物的单克隆抗体及酶联免疫方法和试剂盒。ZL201410830131.2。序3 8. 用于检测甲睾酮的单克隆抗体及酶联免疫方法与试剂盒。ZL201410830027.3。序3 9. 用于检测己烯雌酚的单抗隆抗体及酶联免疫方法和试剂盒。ZL201410830464.5。序3 10. 用于检测雄激素类药物的单克隆抗体及酶联免疫方法及试剂盒。ZL201410829543.4。序3 11. 磺胺类药物分子印迹固相萃取小柱的制备方法及应用。ZL201210049753.2。序3 12. 吉他霉素残留检测用基因工程抗体及制备方法和应用。ZL201210049798.X。序3 13. 磺胺二甲嘧啶分子印迹仿生识别试剂盒及制备方法和应用。ZL201210049767.4。序3 14. 呋喃西林残留标示物氨基脲单克隆抗体及制备方法和应用。ZL201210049821.5。序3 15. 阿苯达唑-2-氨基砜残留检测单克隆抗体及制备方法和应用。ZL201210049837.6。序3 16. 吉他霉素残留检测用单克隆抗体及制备方法和应用。ZL201210049826.8。序3 17. 一种检测磺胺类药物残留的胶体金试纸条及其使用方法和应用。ZL201210 177239.7。序3 18. 一种检测新霉素的胶体金试纸条及其应用。ZL201210176424.4。序3 19. 用于检测氟喹诺酮类药物残留的单克隆抗体及酶联免疫方法与试剂盒。ZL201210176496.9。序3 20. 用于检测阿散酸、硝苯砷酸和卡巴胂的单克隆抗体及酶联免疫方法与试剂盒。ZL201210176485.0。序3 21. 用于检测3-甲基喹噁啉-2-羧酸的单克隆抗体及酶联免疫方法与试剂盒。ZL201210045175.5。序3 22. 用于检测磺胺类药物的单克隆抗体及酶联免疫方法与试剂盒。ZL201210045195.2。序3 23. 检测孔雀石绿、无色孔雀石绿、无色结晶紫的单抗及酶联免疫方法与试剂盒。ZL201210045217.5。序3 24. 泰乐菌素和替米考星残留检测用单克隆抗体及酶联免疫方法与试剂盒。ZL200710052663.8。序3 25. 喹噁啉-2-羧酸残留的酶联免疫检测方法及试剂盒。ZL200610064837.5。序3 26. 用于检测苯乙醇胺A的单克隆抗体及酶联免疫方法与试剂盒。ZL201410830040.9。序4 27. 一种3-甲基喹噁啉-2 羧酸免疫亲和柱及其制备方法。ZL201510018577.X。序4 28. 用于检测ß-受体兴奋剂的单克隆抗体及酶联免疫方法与试剂盒。ZL201210176538.9。序4 29. 用于检测莱克多巴胺的单克隆抗体及酶联免疫方法与试剂盒。ZL201210176468.7。序4 30. 用于检测庆大霉素和西索霉素的单克隆抗体及酶联免疫方法与试剂盒。ZL201210045205.2。序4 31. 用于检测β-胡萝卜素类色素的单克隆抗体及酶联免疫方法与试剂盒。ZL201210045212.2。 32. 用于检测新霉素、阿米卡星和巴龙霉素的单抗和酶联免疫方法与试剂盒。ZL201210045202.9。序4 33. 基于荧光素酶标记工程菌的氟喹诺酮类药物残留检测方法。ZL201310578673.0。序5 34. 3-甲基喹噁啉-2-羧酸残留的酶联免疫检测方法及试剂盒。ZL200610164836.0。序5 科研项目 1. 基于可视化技术的磺胺类和甲氧苄啶类双特异分子识别元件与其靶标分子识别机理研究。国家自然科学基金面上项目(项目编号:31772074),2018-2021, 60万。 2. 种猪生产性能测定与种猪常温精液质量检测4。农产品质量安全监管专项经费项目(农办牧[2017]35号),2016-2017, 8万。 3. 种猪生产性能测定与种猪常温精液质量检测2016(种猪用药安全检测)。农产品质量安全监管(畜牧)项目,2016-2017, 6万。 3. 几种主要霉菌毒素检测产品创制与产业化研究。华中农业大学自主科技创新基金项目(编号2662017PY049),2017-2019, 24万。 4. 几种兽药残留的抗体芯片检测方法研究。华中农业大学自主科技创新基金项目(编号2662015QC030),2015-2016, 8万。 5. 开展高风险性兽药安全性评价及风险评估、兽药休药期试验及制定工作。农业行业标准制修订项目(农财发[2012]34号),2012-2013, 35万。 6. 动物性食品中兽药残留最高限量标准。农业行业标准制修订项目(农财发[2009]105号),2009-2010, 20万。 7. 动物性食品中糖皮质激素类残留检测。农业部2008年农产品质量安全监管专项经费(农财发[2008]91号),2008,133.1万。 8. 喹噁啉类和阿苯哒唑残留标示物标准品制备。十一五国家科技支撑计划《食品安全关键技术》标准品制备课题(2006BAK02A30),2006-2008, 20万。 主要奖励 1. 2016年,动物源食品中主要兽药残留物高效检测关键技术。国家技术发明奖,二等奖,序2. 2. 2016年,喹噁啉类三种兽药的食品安全性评价与风险管控技术研究。湖北省人民政府,科技进步奖,一等奖,序4. 3. 2015年,动物源食品有害残留高效检测核心试剂创制与产品研发。湖北省人民政府,技术发明奖,一等奖,序3. 4. 2015年,兽用抗菌药和违禁药残留高效检测标准制定与产品创制。北京大北农集团,第九届大北农科技成果奖,一等奖,序6. 5. 2013年,兽药残留检测方法标准研究制订。湖北省人民政府,科学技术进步奖,一等奖,序8. 6. 2010年,动物源性食品中糖皮质类药物残留检测液相色谱-串联质谱法。湖北省技术监督局,标准创新贡献奖,一等奖,序6. 7. 2010年,免疫速测技术在禽病诊断与食品安全检测中的应用。湖北省科技进步一等奖,序13. 8. 2008年,兽药残留检测关键技术创新与配套集成研发。教育部高等学校科学研究优秀成果奖科学技术进步奖,一等奖,序5. 9. 2008年,特异性单克隆抗体的制备及其在主要鸡传染病鉴别诊断中的应用。教育部高等学校科学研究优秀成果奖技术发明奖,二等奖,序4.

研究领域

兽药残留与食品安全

近期论文

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1.Dapeng Peng, Lingyan Zhang, Chen Situ, Yuanhu Pan, Yanfei Tao, Yulian Wang*, Zonghui Yuan*. Development of monoclonal antibodies and indirect competitive enzyme-Linked immunosorbent assay kits for the detection of clenbuterol and salbutamol in the tissues and products of food-producing animals. Food Anal. Methods. 2017, 10(11), DOI 10.1007/s12161-017-0925-z IF: 2.038 2.Dapeng Peng#, Zhaozhou Li#, Yulian Wang, Zhenli Liu, Feng Sheng*, Zonghui Yuan*. Enzyme-linked immunoassay based on imprinted microspheres forthe detection of sulfamethazine residue. Journal of Chromatography A, 2017, 1506, 9–17. IF: 3.981 3.Dapeng Peng, Xiya Zhang, Yulian Wang, Yuanhu Pan, Zhenli Liu, Dongmei Chen*, Feng Sheng*, Zonghui Yuan*. An immunoaffinity column for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid from swine tissues and its determination by high-performance liquid chromatography with ultraviolet detection and a colloidal gold-based immunochromatographic assay. Food Chemistry. 2017, 237, 290-296. 4.529 4.Juan Wang, Yulian Wang, Yuanhu Pan, Dongmei Chen, Zhenli Liu, Liang Feng, Dapeng Peng*, Zonghui Yuan*. Preparation of a generic monoclonal antibody and development of a highly sensitive indirect competitive ELISA for the detection of phenothiazines in animal feed. Food Chemistry. 2017, 221: 1004–1013. IF: 4.529 5.Dapeng Peng#, Feng Liao#, Yuanhu Pan, Dongmei Chen, Zhenli Liu, Yulian Wang*, Zonghui Yuan*. Development a monoclonal antibody-based enzyme-linked immunosorbent assay for screening carotenoids in eggs. Food Chemistry. 2016, 202: 141-148. IF: 4.529 6.Dapeng Peng#, Liang Feng#, Yuanhu Pan, Yulian Wang, Dongmei Chen, Juan Wang, Zonghui Yuan*. Development and validation of an indirect competitive enzyme-linked immunosorbent assay for monitoring organoarsenic compounds in edible chicken, pork and feed. Food Chemistry. 2016, 197: 821-828. IF: 4.529 7.Wei Han, Yuanhu Pan, Yulian Wang, Dongmei Chen, Zhenli Liu, Qi Zhou, Liang Feng, Dapeng Peng*, Zonghui Yuan*. Development of a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for nitroimidazoles in edible animal tissues and feeds. Journal of Pharmaceutical and Biomedical Analysis. 2016, 120: 84-91. IF: 3.255 8.Dapeng Peng#, Wei Wei#, Yuanhu Pan, Yulian Wang, Dongmei Chen, Zhenli Liu, Xu Wang, Menghong Dai*, Zonghui Yuan*. Preparation of a monoclonal antibody against amantadine and rimantadine and development of an indirect competitive enzyme-linked immunosorbent assay for detecting the same in chicken muscle and liver. Journal of Pharmaceutical and Biomedical Analysis. 2017, 133: 56–63. IF: 3.255 9.Dapeng Peng#, Bijia Yang#, Yuanhu Pan, Yulian Wang, Dongmei Chen, Zhenli Liu, Wenxiang Yang, Yanfei Tao*, Zonghui Yuan*. Development and validation of a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for the determination of aflatoxin M1 levels in milk. Toxicon, 2016, 113: 18-24. IF: 1.927 10.Dapeng Peng, Liyan Zhang, Changyou Zhai, Yulian Wang, Dongmei Chen, Yanfei Tao, Yuanhu Pan, Zhenli Liu, Zonghui Yuan*. Development and validation of a monoclonal antibody-based indirect competitive ELISA for the detection of sudan I in duck eggs and crystal violet in carp. Food Analytical Methods, 2017, 10(5):1442-1451. IF: 2.038 11.Dapeng Peng, Yulian Wang, Liang Feng, Guangcai Cao, Yanfei Tao, Zhenli Liu, Zonghui Yuan*. Preparation of Broadly Specific Monoclonal Antibodies for Simultaneous Determination of Fluoroquinolone Residues in Eggs. Food Analytical Methods, 2016, 9(12):3520-3531. IF: 2.038 12.Juan Wang, Yulian Wang, Yuanhu Pan, Liang Feng, Dongmei Chen, Zhenli Liu, Dapeng Peng*, Zonghui Yuan*. Preparation of a Broadly Specific Monoclonal Antibody-Based Indirect Competitive ELISA for the Detection of Benzodiazepines in Edible Animal Tissues and Feed. Food Analytical Methods, 2016, 9(12): 3407-3419. IF: 2.038 13.Lingling An, Yulian Wang, Yuanhu Pan, Yanfei Tao, Dongmei Chen, Zhenli Liu, Wenxiang Yang, Dapeng Peng*, Zonghui Yuan*. Development and validation of a sensitive indirect competitive enzyme-Linked immunosorbent assay for the screening of florfenicol and thiamphenicol in edible animal tissue and feed. Food Analytical Methods, 2016, 9(9):2434-2443. IF: 2.038 14.Dapeng Peng#, Fangfang Chang#, Yulian Wang, Dongmei Chen, Zhenli Liu, Xiaodong Zhou, Liang Feng, Zonghui Yuan*. Development of a sensitive monoclonal-based enzyme-linked immunosorbent assay for monitoring T-2 toxin in food and feed. Food Additives & Contaminants: Part A, 2016, 33(4): 683-692 IF: 2.047 15.Dapeng Peng, Nenghui Jiang, Yulian Wang, Dongmei Chen, Zhenli Liu, Zonghui Yuan*. Development and validation of an indirect competitive enzyme-linked immunosorbent assay for the detection of albendazole 2-aminosulfone residues in animal tissues. Food and Agricultural Immunology 2016; 27(2): 273-287. IF: 1.392 16.Xiya Zhang#, Dapeng Peng#, Yuanhu Pan, Yulian Wang, Dongmei Chen, Qi Zhou, Zhenli Liu, Zonghui Yuan*. A novel hapten and monoclonal-based enzyme-linked immunosorbent assay for 3-methyl-quinoxaline-2-carboxylic acid in edible animal tissues. Analytical Methods, 2015, 7: 6588–6594. IF: 1.900 17.Qi Zhou#, Dapeng Peng#, Yulian Wang, Yuanhu Pan, Dan Wan, Xiya Zhang, Zonghui Yuan*. A novel haptene and monoclonal-based enzyme-linked immunosorbent assay for sulfonamides in edible animal tissues. Food Chemistry. 2014, 154: 52-56. IF: 4.529 18.Dapeng Peng#, Shengqiang Ye#, Yulian Wang, Dongmei Chen, Yanfei Tao, Lingli Huang, Zhenli Liu, Menghong Dai, Xiaoqing Wang, and Zonghui Yuan*. Development and validation of an indirect competitive enzyme-linked immunosorbent assay for the screening of tylosin and tilmicosin in muscle, liver, milk, honey and eggs. Journal of Agricultural and Food Chemistry, 2012, 60: 44-51. 2012 Jan 11 IF: 3.154 Dapeng Peng, Zeying Zhang, Dongmei Chen, Yulian Wang, Yanfei Tao, Zonghui Yuan*. Development and validation of an indirect competitive enzyme-linked immunosorbent assay for monitoring quinoxaline-2-carboxylic acid in the edible tissues of animals. Food Additives and Contaminants: Part A, 2011, 28(11): 1524-1533. IF: 2.047 19.Yu Liu, Dapeng Peng, Lingli Huang, Yulian Wang, Chao Chang, Awais Ihsan, Yanfei Tao, Bo Yang, Zonghui Yuan*. Application of a modified enzyme-linked immunosorbent assay for 3-amino-2-oxazolidinone residue in aquatic animals. Analytica Chimica Acta, 2010, 664(2): 151-157. IF: 4.950 20.Chunbao Zhao#, Dapeng Peng#, Yulian Wang, Lingli Huang, Dongmei Chen, Yanfei Tao and Zonghui Yuan*. Preparation and validation of the polyclonal antibodies for detection of chlortetracycline residues. Food and Agricultural Immunology, 2008, 19(2): 163-174 IF: 1.392 21.Chang Chao#, Dapeng Peng#, Jine Wu, Yulian Wang, Zonghui Yuan*. Development of an indirect competitive ELISA for detecting furazolidone marker residue in animal edible tissues. Journal of Agricultural and Food Chemistry, 2008, 56(5): 1525-1531. IF: 3.154 Dapeng Peng#, Sishun Hu#, Yan Hua, Yuncai Xiao, Zili Li, Xiliang Wang, Dingren Bi*. Comparison of a new gold-immunochromatographic assay for the detection of antibodies against avian influenza virus with hemagglutination inhibition and agar gel immunodiffusion assays. Veterinary Immunology and Immunopathology, 2007, 117(1-2): 17-25. IF:1.718

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