研究领域

Organic and Biological Chemistry

Research in our group involves the use of a broad range of techniques including protein expression and purification, enzyme kinetics, and the accurate mass analysis of these large biomolecules by electrospray mass spectrometry. By isolating individual enzymes and establishing structural details, enzyme specificities, and kinetic characteristics we hope to understand fully how biosynthetic pathways are controlled.

近期论文

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Dong, X, Bailey, C, Williams, C, Crosby, J, Simpson, T, Willis, C & Crump, M, 2015, ‘Recognition of extended linear and cyclised polyketide mimics by a Type II acyl carrier protein’. Chemical Science, vol 7., pp. 1779-1785 Javidpour, P, Bruegger, J, Srithahan, S, Korman, TP, Crump, MP, Crosby, J, Burkart, MD & Tsai, S-C, 2013, ‘The Determinants of Activity and Specificity in Actinorhodin Type II Polyketide Ketoreductase’. Chemistry & Biology, vol 20., pp. 1225-1234 Murphy, AC, Gao, S-S, Han, L-C, Carobene, S, Fukuda, D, Song, Z, Hothersall, J, Cox, RJ, Crosby, J, Crump, MP, Thomas, CM, Willis, CL & Simpson, TJ, 2013, ‘Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp SANK 73390’. Chemical Science, vol 5., pp. 397-402 Haines, AS, Dong, X, Song, Z, Farmer, R, Williams, C, Hothersall, J, Ploskon, E, Wattana-amorn, P, Stephens, ER, Yamada, E, Gurney, R, Takebayashi, Y, Masschelein, J, Cox, RJ, Lavigne, R, Willis, CL, Simpson, TJ, Crosby, J, Winn, PJ, Thomas, CM & Crump, MP, 2013, ‘A conserved motif flags acyl carrier proteins for β-branching in polyketide synthesis’. Nature Chemical Biology, vol 9., pp. 685-692 Maple, HJ, Crosby, J & Crump, MP, 2013, ‘Identification of Differential Protein Binding Affinities in an Atropisomeric Pharmaceutical Compound by Noncovalent Mass Spectrometry, Equilibrium Dialysis, and Nuclear Magnetic Resonance’. Analytical Chemistry. Crosby, J & Crump, MP, 2012, ‘The structural role of the carrier protein - active controller or passive carrier’. Natural Product Reports, vol 29., pp. 1111-1137 Crosby, J, Treadwell, T, Hammerton, M, Vasilakis, K, Crump, MP, Williams, DS & Mann, S, 2012, ‘Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets’. Chemical Engineering Communications, vol 48., pp. 11832-11834 Maple, HJ, Garlish, R, Rigau-Roca, L, Porter, J, Whitcombe, I, Prosser, C, Kennedy, J, Henry, A, Taylor, R, Crump, M & Crosby, J, 2012, ‘Automated protein-ligand interaction screening by mass spectrometry’. Journal of Medicinal Chemistry, vol 55., pp. 837-851 Fukuda, D, Haines, AS, Song, Z, Murphy, AC, Hothersall, J, Stephens, ER, Gurney, R, Cox, RJ, Crosby, J, Willis, CL, Simpson, TJ & Thomas, CM, 2011, ‘A Natural Plasmid Uniquely Encodes Two Biosynthetic Pathways Creating a Potent Anti-MRSA Antibiotic’. Plos one, vol 6., pp. - Hothersall, J, Murphy, AC, Iqbal, Z, Campbell, G, Stephens, ER, Wu, J, Cooper, H, Atkinson, S, Williams, P, Crosby, J, Willis, CL, Cox, RJ, Simpson, TJ & Thomas, CM, 2011, ‘Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin production’. Applied Microbiology and Biotechnology, vol 90., pp. 1017-1026 Dall'Aglio, P, Arthur, C, Williams, C, Vasilakis, K, Maple, HJ, Crosby, J, Crump, MP & Hadfield, AT, 2011, ‘Analysis of Streptomyces coelicolor Phosphopantetheinyl Transferase, AcpS, Reveals the Basis for Relaxed Substrate Specificity’. Biochemistry, vol 50., pp. 5704-5717 Shields, J, Rahman, A, Arthur, C, Crosby, J, Hothersall, J, Simpson, T & Thomas, C, 2010, ‘Phosphopantetheinylation and Specificity of Acyl Carrier Proteins in the Mupirocin Biosynthetic Cluster’. ChemBioChem, vol 11., pp. 148 - 255