Overton, Tim - University of Birmingham - School of Chemical Engineering

个人简介

Prior to joining Chemical Engineering, Dr Overton was a postdoctoral researcher in the School of Biosciences at the University of Birmingham, studying gene regulation in both model organisms (Escherichia coli) and human pathogens (Neisseria gonorrhoeae and E. coli O157) in response to oxygen and reactive species using transcriptomic, proteomic and other molecular biology techniques. Using systems biology and other molecular approaches, he identified mechanisms for bacterial survival in adverse environments. Research became focused on bioprocessing topics during a BBSRC-EPSRC Bioprocessing Research Industry Club (BRIC) grant in collaboration with GSK, studying the production of difficult recombinant proteins in E. coli. Dr Overton was initially postdoctoral researcher on this project, and moved to a Co-I role upon taking up his position in the School of Chemical Engineering. Current research extends previous work on stress and recombinant protein production, and has developed in several new areas (detailed below). Dr Overton is director of the MSc in Biochemical Engineering and chairs the School MSc and EngD board of examiners. He is a member of the University Advisory Group on Biological Hazards and is School GMO safety officer and a member of the School safety committee.

近期论文

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Chaudhari RD, Stenson JD, Overton TW, Thomas CR. (2012) Effect of Bud Scars on the Mechanical Properties of Saccharomyces cerevisiae Cell Walls. Chemical Engineering Science 84: 188-196 Foulkes, JM, Winn, M, Perni, S, Simmons, MJH, Overton, TW & Goss, RJM. (2012) Biofilms and their Engineered Counterparts: a new Generation of Immobilised Biocatalysts. Catalysis Science and Technology DOI: 10.1039/C2CY20085F Vizcaino-Caston, I, Wyre, C and Overton, TW. (2012) Fluorescent proteins in microbial biotechnology – new proteins and new applications. Biotechnology Letters 34: 175-186. Tsoligkas, AN, Bowen, J, Winn, M, Goss, RJM, Overton, TW and Simmons, MJH. (2012) Characterisation of spin coated engineered Escherichia coli biofilms using atomic force microscopy. Colloids and Surfaces B: Biointerfaces 89: 152-160. Whitehead, RN, Kemp, CL, Overton, TW and Webber, MA. (2011) Exposure of Salmonella enterica serovar Typhimurium to high level biocide challenge can select for multidrug resistant mutants in a single step. PLoS ONE 6(7): e22833 doi:10.1371/journal.pone.0022833. Tsoligkas, AN, Winn, M, Bowen, J, Overton, TW, Simmons, MJH, and Goss, RJM. (2011) Engineering Biofilms for Biocatalysis. ChemBioChem 12: 1391-1395. Li, Y, Hopper, A, Overton, T, Squire, DJ, Cole, J, and Tovell ,N. (2010) Organisation of the electron transfer chain to oxygen in the obligate human pathogen, Neisseria gonorrhoeae: roles for cytochromes c4 and c5, but not cytochrome c2, in oxygen reduction. Journal of Bacteriology 192: 2395-406. Yanina Sevastsyanovich, Y, Alfasi, S, Overton, T, Hall, R, Jones, J, Hewitt, C, and Cole, J. (2009) Exploitation of GFP fusion proteins and stress avoidance as a generic strategy for the production of high-quality recombinant proteins.FEMS Microbiology Letters 299 86–94. Overton, TW, Justino MC, Li Y, Baptista JM, Melo AM, Cole JA, and Saraiva LM. (2008) Widespread distribution in pathogenic bacteria of di-iron proteins that repair oxidative and nitrosative damage to iron-sulfur centers.Journal of Bacteriology 190 2004-132004-13. Overton, TW, Whitehead, R, Li, Y, Griffiths, L and Cole, J. (2008) Sense and nonsense from whole genome microarray data in the analysis of microbial physiology. Biotechnologia 80: 15-30 Filenko, N, Spiro, S, Browning, DF, Squire, D, Overton, TW, Cole, J and Constantinidou, C. (2007) The NsrR regulon of Escherichia coli K-12 includes genes encoding the hybrid cluster protein and the periplasmic, respiratory nitrite reductase. Journal of Bacteriology 189 4410-7. Whitehead, RN, Overton, TW, Snyder LAS, McGowan, S, Smith, H, Cole, JA and Saunders, NJ. (2007) The small FNR regulon of Neisseria gonorrhoeae: comparison with the larger E. coli FNR regulon and interaction with the NarQ-NarP regulon. BMC Genomics 8:35. Nilavongse, A, Brondijk, THC, Overton, TW, Richardson, DJ, Leach, E and Cole, JA (2006) The NapF protein of the Escherichia coli periplasmic nitrate reductase system: demonstration of a cytoplasmic location and interaction with the catalytic subunit, NapA. Microbiology 152 3227-3237 3227-3237. Overton, TW, Whitehead, RN, Li, Y, Snyder, LAS, Saunders, NJ, Smith, H and Cole, JA. (2006) Coordinated regulation of the Neisseria gonorrhoeae truncated denitrification pathway by the nitric oxide-sensitive repressor, NsrR, and nitrite-insensitive NarQ-NarP. truncated denitrification pathway by the nitric oxide-sensitive repressor, NsrR, and nitrite-insensitive NarQ-NarP. Journal of Biological Chemistry 281 33115-33126. Constantinidou, C, Hobman, JL, Griffiths, L, Patel, MD, Penn, CW, Cole, JA and Overton, TW. (2006) A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL and NarQP as Escherichia coli K-12 adapts from aerobic to anaerobic growth. Journal of Biological Chemistry 281 4802-15. Turner, SM, Overton, T, Moir, JW & Cole, JA (2005). Mutational and biochemical analysis of cytochrome c', a nitric oxide-binding lipoprotein important for adaptation of Neisseria gonorrhoeae to oxygen-limited growth. Biochemical Journal 388 545-53. Spreadbury, CL, Pallen, MJ, Overton, T, Behr, MA, Mostowy, S, Spiro, S, Busby, SJ & Cole, JA. (2005) Point Mutations in the DNA- and cNMP-Binding Domains of the Homologue of the cAMP Receptor Protein (CRP) in Mycobacterium bovis BCG: Implications for the Inactivation of a Global Regulator and Strain Attenuation. Microbiology 151 547-446. Grainger, D, Overton, T, Hobman, JL, Constaninidou, C, Tamai, E, Wade, JT, Struhl, K, Reppas, N, Church, G & Busby, SJW (2004). Genomic studies with Escherichia coli MelR protein: applications of chromatin immunoprecipitation and microarrays. Journal of Bacteriology 186 (20) 6938-43.