个人简介

M.S., Federal Institute of Technology in Zurich; Ph.D., University of Basel, 1995

研究领域

Analytical Chemistry/Biomolecular Structure and Function/Chemical and Biological Imaging/Chemical Biology/Inorganic Chemistry/Materials Chemistry/Molecular Biophysics/Organic Chemistry/Spectroscopy and Dynamics/Synthesis

Metals ions play an important role in all forms of life. Approximately 40% of human proteins require one or multiple metal ions for their proper function. These metalloproteins support a wide range of biological processes, including signal transduction, gene regulation, and catalysis. While the function of metal cofactors in proteins have been a longstanding theme in bioinorganic research, the mechanisms by which cells acquire, store, and regulate metal cations remain poorly understood. The importance of this research area is underscored by the discovery of an increasing number of diseases, such as hemochromatosis, Menkes syndrome, and Alzheimer’s disease, that are associated with impaired metal transport and regulation. Our research is concerned with the development and application of molecular tools that will aid in elucidating the transport, storage, and trafficking of transition metal ions in cells, tissues, and whole organisms.

近期论文

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M.T. Morgan, S. Sumalekshmy, M. Sarwar, H. Beck, S. Crooke, C.J. Fahrni, "Probing Ternary Complex Equilibria of Crown Ether Ligands by Time-Resolved Fluorescence Spectroscopy", J. Phys. Chem. B 2014, 118, 14196-14202. D. Bourassa, S.-C. Gleber, S. Vogt, H. Yi, F. Will, H. Richter, C.H. Shin, C.J. Fahrni, "3D Imaging of Transition Metals in the Zebrafish Embryo by X-ray Fluorescence Microtomography”, Metallomics 2014, 6, 1648-1655. P. Bagchi, M.T. Morgan, J. Bacsa, C.J. Fahrni, “Robust affinity standards for Cu(I) biochemistry”, J. Am. Chem. Soc. 2013, 135, 18549-59. McRae, R.; Lai, B.; Fahrni, C. J., "Subcellular redistribution and mitotic inheritance of transition metals in proliferating mouse fibroblast cells.", Metallomics 2013, 5, 52-61. Fahrni, C. J., "Synthetic fluorescent probes for monovalent copper.", Curr. Opin. Chem. Biol. 2013, 17, 656-62 Morgan, M. T.; Bagchi, P.; Fahrni, C. J., "High-contrast fluorescence sensing of aqueous Cu(I) with triarylpyrazoline probes: dissecting the roles of ligand donor strength and excited state proton transfer.", Dalton Trans. 2013, 42, 3240-3248. Nekongo, E. E.; Bagchi, P.; Fahrni, C. J.; Popik, V. V., "9-Aryl-9-xanthenols: a convenient platform for the design of fluorimetric and colorimetric pH indicators.", Org. Biomol. Chem. 2012, 10, 9214-9218. Jablonski, A.; Hsiang, J. C.; Bagchi, P.; Hull, N.; Richards, C. I.; Fahrni, C. J.; Dickson, R. M., "Signal discrimination between fluorescent proteins in live cells by long-wavelength optical modulation", J. Phys. Chem. Lett. 2012, 3, 3585-3591. Friscourt, F.; Fahrni, C. J.; Boons, G.-J., "A fluorogenic probe for the catalyst-free detection of azide-tagged molecules.", J. Am. Chem. Soc. 2012, 134, 18809-18815. Sumalekshmy, S.; Fahrni, C. J., "Metal-ion-responsive fluorescent probes for two-photon excitation microscopy", Chem. Mat. 2011, 823-830. Morgan, M. T.; Bagchi, P.; Fahrni, C. J., "Designed to dissolve: Suppression of colloidal aggregation of Cu(I)-selective fluorescent probes in aqueous buffer and in-gel detection of a metallochaperone.", J. Am. Chem. Soc. 2011, 133, 15906-15909. Chaudhry, A. F.; Mandal, S.; Hardcastle, K. I.; Fahrni, C. J., "High-contrast Cu(I)-selective fluorescent probes based on synergistic electronic and conformational switching", Chem. Sci. 2011, 2, 1016-1024. Verma, M.; Chaudhry, A. F.; Morgan, M. T.; Fahrni, C. J., "Electronically tuned 1,3,5-triarylpyrazolines as Cu(I)-selective fluorescent probes", Org. Biomol. Chem. 2010, 8, 363-370. McRae, R.; Lai, B.; Fahrni, C. J., "Copper redistribution in Atox1-deficient mouse fibroblast cells", J. Biol. Inorg. Chem. 2010, 15, 99-105. Chaudhry, A. F.; Verma, M.; Morgan, M. T.; Henary, M. M.; Siegel, N.; Hales, J. M.; Perry, J. W.; Fahrni, C. J., "Kinetically Controlled Photoinduced Electron Transfer Switching in Cu(I)-Responsive Fluorescent Probes", J. Am. Chem. Soc. 2010, 132, 737-747. Verma, M.; Chaudhry, A. F.; Fahrni, C. J., "Predicting the photoinduced electron transfer thermodynamics in polyfluorinated 1,3,5-triarylpyrazolines based on multiple linear free energy relationships", Org. Biomol. Chem. 2009, 7, 1536-1546. McRae, R.; Bagchi, P.; Sumalekshmy, S.; Fahrni, C. J., "In situ imaging of metals in cells and tissues", Chem. Rev. 2009, 109, 4780-4827. Fahrni, C. J., "Fluorescent probes and labels for cellular imaging", Chimia 2009, 63, 714-720. Solntsev, K. M.; McGrier, P. L.; Fahrni, C. J.; Tolbert, L. M.; Bunz, U. H. F., "Anomalous photophysics of bis(hydroxystyryl)benzenes: A twist on the para/meta dichotomy", Org. Lett. 2008, 10, 2429-2432. McRae, R. L.; Phillips, R. L.; Kim, I. B.; Bunz, U. H. F.; Fahrni, C. J., "Molecular recognition based on low-affinity polyvalent interactions: Selective binding of a carboxylated polymer to fibronectin fibrils of live fibroblast cells", J. Am. Chem. Soc. 2008, 130, 7851-7853.