Chiang, Cheng-Ming - UT Southwestern Medical Center

个人简介

Biography Cheng-Ming Chiang was born in Taipei, Taiwan in 1961. He received his BS from the Department of Agricultural Chemistry, National Taiwan University, in 1984, and his PhD from the Department of Biochemistry, University of Rochester, in 1991. His doctoral dissertation was supervised by Professors Louise Chow and Thomas Broker. After finishing his postdoctoral training with Professor Robert Roeder at Rockefeller University, he took a faculty position in the Department of Biochemistry at University of Illinois, Urbana-Champaign in 1995. In 2000, he moved to Department of Biochemistry at Case Western Reserve University as tenured Associate Professor. Dr. Chiang accepted a Professorship at UT Southwestern and relocated his laboratory to UT Southwestern in July 2007. Dr. Chiang is a 1996 Pew Scholar and has been an editorial board member of the Journal of Biological Chemistry and Faculty of 1000 since 2003. He has been a regular member for American Cancer Society Genetic/DNA Mechanisms in Cancer, National Institutes of Health Virology B, and Human Frontier Science Program study sections. His research interests span transcription, chromatin, gene regulation, and virology. Education Undergraduate National Taiwan University , Chemistry Graduate School University of Rochester , Biochemistry Honors & Awards Mt. Sinai Health Care Foundation Scholar Case Western Reserve University (2000) Pew Scholar in the Biomedical Sciences Pew Charitable Trust (1996) Postdoctoral Fellow Helen Hay Whitney Foundation (1993) Postdoctoral Fellow Aaron Diamond Foundation (1993) The Wallace O. Fenn Award University of Rochester (1991)

研究领域

My lab has been interested in understanding the mechanisms of transcription and gene regulation in eukaryotes using primarily cell-free systems reconstituted with purified gene-specific transcription factors, general cofactors, and components of the general transcription machinery to recapitulate transcriptional events in the test tubes. The mechanistic studies based on cell-free transcription systems with DNA and chromatin templates are further verified in vivo by chromatin immunoprecipitation (ChIP), RT-PCR, reporter gene assay, co-immunoprecipitation (co-IP), and nucleosome mapping using different types of cultured cells, including knock-out and knock-down cells. Our goals are to elucidate the general principles underlying gene activation and repression in mammalian cells and their associated viruses, in particular, DNA tumor viruses such as human papillomaviruses (HPVs). Recently, we have applied reconstituted chromatin systems to define histone modifications and chromatin remodeling involved in p53 target gene transcription implicated in cell cycle control, DNA repair, and apoptosis. In addition, we have identified bromodomain 4 protein (Brd4) as the cellular corepressor implicated in transcriptional repression of HPV E6 and E7 oncoprotein expression by HPV-encoded E2 proteins. In general, our research has been focused on the following four areas: Research Interest Brd4 in Epigenetic Control and Cancer Therapeutics Chromatin Transcription and Posttranslational Modification Gene Regulation in Human Papillomaviruses (HPVs) Human General Transcription Factors and Cofactors in Eukaryotic Transcription p53 Tumor Suppressor Function and Regulation

近期论文

查看导师最新文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Phospho Switch Triggers Brd4 Chromatin Binding and Activator Recruitment for Gene-Specific Targeting. Wu SY, Lee AY, Lai HT, Zhang H, Chiang CM Mol. Cell 2013 Jan p53-Aurora A mitotic feedback loop regulates cell cycle progression and genomic stability. Chiang CM Cell Cycle 2012 Oct 11 20 3719-20 Binding site specificity and factor redundancy in activator protein-1-driven human papillomavirus chromatin-dependent transcription. Wang WM, Wu SY, Lee AY, Chiang CM J. Biol. Chem. 2011 Nov 286 47 40974-86 Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding. Wu SY, Chiang CM EMBO J. 2009 May 28 9 1246-59 Chromatin adaptor Brd4 modulates E2 transcription activity and protein stability. Lee AY, Chiang CM J. Biol. Chem. 2009 Jan 284 5 2778-86 Brd4 engagement from chromatin targeting to transcriptional regulation: selective contact with acetylated histone H3 and H4. Chiang CM F1000 Biol Rep 2009 1 98 The double bromodomain-containing chromatin adaptor Brd4 and transcriptional regulation. Wu SY, Chiang CM J. Biol. Chem. 2007 May 282 18 13141-5 Brd4 links chromatin targeting to HPV transcriptional silencing. Wu SY, Lee AY, Hou SY, Kemper JK, Erdjument-Bromage H, Tempst P, Chiang CM Genes Dev. 2006 Sep 20 17 2383-96 The general transcription machinery and general cofactors. Thomas MC, Chiang CM Crit. Rev. Biochem. Mol. Biol. 2006 May-Jun 41 3 105-78 E6 oncoprotein represses p53-dependent gene activation via inhibition of protein acetylation independently of inducing p53 degradation. Thomas MC, Chiang CM Mol. Cell 2005 Jan 17 2 251-64 p53 sumoylation: mechanistic insights from reconstitution studies. Wu SY, Chiang CM Epigenetics 2009 Oct 4 7 445-51 One-step affinity tag purification of full-length recombinant human AP-1 complexes from bacterial inclusion bodies using a polycistronic expression system. Wang WM, Lee AY, Chiang CM Protein Expr. Purif. 2008 May 59 1 144-52 Human mediator enhances activator-facilitated recruitment of RNA polymerase II and promoter recognition by TATA-binding protein (TBP) independently of TBP-associated factors. Wu SY, Zhou T, Chiang CM Mol. Cell. Biol. 2003 Sep 23 17 6229-42