个人简介
工作经历:
2015/8- 华侨大学生物医学学院讲师,硕士研究生导师
研究课题:
1. 华侨大学科研启动费项目-引进人才类(15BS306),基于CRISPR - Cas9基因敲入技术的斑马鱼血栓模型的建立,4万元,在研,主持;
2. 国家自然科学基金青年项目(31801034):斑马鱼精巢特异表达基因lrp2bp对雄激素11-KT合成及分泌的调控机制研究,23万元,在研,主持;
3. 国家自然科学基金青年项目(31700071),基于嗜盐古菌phaP/phaR重组表达的PHA颗粒粒径控制研究,22万元,在研,参加。
专利申请:
1. 李文华,马国兴,吴雅琴,徐嵘,许瑞安。一种方格星虫β-肌动蛋白基因及其应用(201810801904.2)。
2. 李文华,吴雅琴,陈琪薇。一种用于鉴定方格星虫遗传性别的探针及方法(201911036273.0)。
研究领域
1. 斑马鱼和方格星虫发育遗传学
2. 斑马鱼逆境发育生物学
近期论文
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1. Wenhua Li, Li Zhou, Zhi Li, Yang Wang, Jiantao Shi, Yanjing Yang, Jianfang Gui. Zebrafish Lbh-like is required for Otx2-mediated photoreceptor differentiation. International Journal of Biological Sciences 2015, 11: 688-700.
2. Yaqin Wu#, Wenhua Li#*, Mingrui Yuan, Xuan Liu. The synthetic pyrethroid deltamethrin impairs zebrafish (Danio rerio) swim bladder development. Science of the Total Environment 2020, 701: 134870. (#共同一作)
3. Wenhua Li*, Mingrui Yuan, Yaqin Wu. In situ hybridization for Sipunculus nudus coelomic fluid. Journal of Visualized Experiments 2020, e61022.
4. Wenhua Li, Mingrui Yuan, Yaqin Wu, Ruian Xu*. Identification of genes expressed differentially in female and male gametes of Sipunculus nudus. Aquaculture Research 2020. (Accepted)
5. Wenhua Li*, Yaqin Wu, Mingrui Yuan, Xuan Liu*. Fluxapyroxad induces developmental delay in zebrafish (Danio rerio). Chemosphere 2020, 127037.
6. 李文华,汪洋,李志,桂建芳,周莉。银鲫转录调控因子lbh-b cDNA克隆与表达分析。水生生物学报,2018,42(4): 673-680.
7. 李文华,吴雅琴,马国兴,袁铭瑞,许瑞安。方格星虫dmrt1基因的克隆和表达分析。水生生物学报,2019,43(6): 1210-1215.
8. Yang Wang, Wenhua Li, Zhi Li, Wei Liu, Li Zhou, Jianfang Gui*. BMP and RA signaling cooperate to regulate Apolipoprotein C1 expression during embryonic development. Gene 2014, 554:196-204.
9. Shizhu Li, Wei Liu, Zhi Li, Wenhua Li, Yang Wang, Li Zhou, Jianfang Gui*. Greb1 regulates convergent extension movement and pituitary development in zebrafish. Gene 2017, 627:176-187.
10. Yang Wang, Li Zhou, Zhi Li, Wenhua Li, Jianfang Gui*. Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish. Science China Life Science 2013, 56:975-984.
11. Peng Xiao, Yongguang Jiang, Yang Liu, Wenhua Tan, Wenhua Li, Renhui Li*. Re-evaluation of the diversity and distribution of diazotrophs in the South China Sea by pyrosequencing the nifH gene. Marine and Freshwater Research 2015, 66:681-691.