个人简介

2001 Research Corporation Research Innovation Award 2005 Present Faculty of 1000 Biology, Faculty Member, Chemical Biology Division

研究领域

Biochemistry/Biofuels and Biomanufacturing/Biomolecular Structure and Function/Catalysis Chemical Biology/Molecular Biophysics/Organic Chemistry/Physical Chemistry/Synthesis

Engineering Nuclear Hormone Receptors. Nuclear hormone receptors control the expression of genes in response to small molecule hormones. In performing this activity, the receptors must specifically recognize small molecules, DNA, and other proteins. The amino acids that recognize each of these substrates are varied using genetic engineering techniques until a receptor with novel recognition is created. The original and new receptors are studied using a variety of biophysical techniques to elucidate the principles behind the new activity. This exercise provides both new knowledge for future protein engineering and real materials for research and medical applications. We engineer new receptors to turn on gene expression in response to specifically chosen small molecules. The small molecule may be chosen for its properties in the specific application. For applications in gene therapy and use as a tailored gene switch, the small molecule may be chosen due to its long or short pharmacokinetic half-life, because it crosses the blood-brain barrier, or other tissue specificity. For applications in synthetic biology gene circuits, the small molecule may be chosen for its interactions with other circuits in a network. Another application is in assembling the biosynthetic components (enzymes) to synthesize the small molecule via genetic selection.

近期论文

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Cellular antioxidant activity of phenylaminoethyl selenides as monitored by chemiluminescence of peroxalate nanoparticles and by reduction of lipopolysaccharide-induced oxidative stress. Cowan EA, Taylor JL, Oldham CD, Dasari M, Doyle D, Murthy N, May SW Enzyme Microb. Technol.. 2013, 53, 373-377. "Histone Deacetylase Inhibitors Equipped with Estrogen Receptor Modulation Activity". Gryder BE, Rood MK, Johnson KA, Patil V, Raftery ED, Yao LP, Rice M, Azizi B, Doyle DF, and Oyelere AK J Med Chem. 2013, 56, 5782–5796. "BAPJ69-4A: A yeast two-hybrid strain for both positive and negative genetic selection.". Shaffer, H.A., Rood, M.K., Kashlan, B., Chang, E.I., Doyle, D.F., Azizi, B. J Microbiol Methods . 2012, 91, 22-29. “A human vitamin D receptor mutant activated by cholecalciferol.”. Ousley, A.M., Castillo, H.S., Duraj-Thatte, A., Doyle, D.F. and Azizi, B. J Steroid Biochem Mol Biol . 2011, 125, 3-5. “Characterization of a molecular switch system that regulates gene expression in mammalian cells through a small molecule”. Taylor, J.T., Rohatgi, P., Spencer, H.T., Doyle, D.F., and Azizi, B., BMC Biotechnology . 2010, 10, 15.