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华南师范大学生物光子学研究院院长、学术委员会主任,激光生命科学教育部重点实验室教授,博士生导师,广东省“千百十人才工程”省级培养对象。主持国家863项目1项(230万)、国家重大科研仪器研制项目1项(510万)、国家自然科学基金面上项目5项,主持广东科技计划项目1项、重点项目1项以及其他省部级项目4项。研究领域主要涉及生物医学光子学、生物物理学、肿瘤细胞生物学、分子生物学。发表学术论文140余篇,获得授权国家发明专利5项。 教育简历: 1982.9-1986.6:湖北师范学院物理专业毕业,获理学学士学位; 1990.9-1993.5:华中科技大学光电子与物理电子专业毕业,获理学硕士学位; 1999.9-2003.5:华中科技大学生物医学工程专业毕业,获工学博士学位; 工作简历: 1986.6-1990.9:湖北师范学院物理系教师,助教; 1993.9-2000.9:湖北师范学院物理系教师,讲师; 2000.9-2003.11:华中科技大学生命科学院,副教授; 2003.11-2005.12:华南师范大学,副教授, 副所长; 2005.12-2013: 华南师范大学激光生命科学教育部重点实验室 教授,博士导师; 2013-2018.06: 华南师范大学生物光子学研究院学术委员会主任,教授,博导; 2018.07-至今: 华南师范大学生物光子学研究院院长、学术委员会主任,教授,博导; 主持承担的主要基金项目 1. 国家重大科研仪器研制项目(主持):定量FRET显微成像仪的研制(61527825),研究总经费510.32万元,直接经费442.1万元,起止年月:2016.01-2020.12 2. 国家自然科学基金面上项目(主持):基于线性光谱分离的活细胞实时定量FRET显微成像技术研究(81471699) 80万,起止年月:2015.01-2018.12 3. 广东省科技计划项目(主持):活细胞FRET光谱定量检测仪的研制(2014B090901060),100万,起止年月:2015.01-2018.12 4. 广州市科技计划项目(主持):光谱FRET定量检测分析技术研究(2014J4100055), 8万元,起止年月:2014.04-2016.04 5. 国家自然科学基金面上项目(主持):活细胞中蛋白质寡聚化动态过程实时荧光成像与定量分析技术研究(61178078),78万元,起止年月:2012.1-2015.12. 6. 国家自然科学基金面上项目(主持):显微光学成像术研究紫杉醇诱导肿瘤细胞内质网空泡化和类副凋亡的分子调控机理(31071218),35万元,起止年月:2011.1-2013.12. 7. 广东省高教厅、财政厅重点项目(主持):非小细胞肺癌对吉非替尼耐药分子机制的单细胞可视化研究(cxzd115),25万元,起止年月:2011.10-2014.10。 8. 国家自然科学基金面上项目(主持):荧光技术在活细胞中实时研究中药诱导肿瘤细胞凋亡的分子机理(30670507),28万元,起止年月:2007.1-2009.12。 主要获奖 (1) 组织光学成像理论若干关键问题研究,2003年度湖北省自然科学二等奖,骆清铭,曾绍群,朱丹,吴龟灵,陈同生。 (2) 光学与生命科学交叉科学中的前沿问题与关键技术研究.2009年度广东省科学技术奖(自然科学类)二等奖,邢达,朱德斌,陈同生,章春笋 张玲瑞,刘镭,唐永红等。

研究领域

生物医学光子学、细胞生物学

近期论文

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1 Hai-yang Li, Cai-ping Zhuang, Xiao-ping Wang,Tong-sheng Chen*. SB203580 enhances the RV-induced loss of mitochondrial membrane potential and apoptosis in A549 cells. Proc. of SPIE 2012, 8224, 82240L(EI收录) 2 Chao-yang Wang,Tong-sheng Chen*.Taxol-induced paraptosis-like A549 cell death is not senescence. Proc. of SPIE 2011, 7900: 79000K-1(EI收录) 3 Huai-na Yu, Ying-ying Lu,Tong-sheng Chen*. Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells. Proc. of SPIE 2011, 7900: 79000J-1(EI收录) 4 Wen-jing Guo,Tong-sheng Chen*. Taxol induces concentration-dependent phosphatidylserine (PS) externalization and cell cycle arrest in ASTC-a-1 cells. Proceedings of the SPIE, 7565:75650J-75650J-7 (2010). DOI: 10.1117/12.839552 (EI收录) 5 Feng-lian Xiao,Tong-sheng Chen*, Jun-Le Qu, Cheng-Yi Liu. Artemisinin induces ROS-mediated caspase3 activation in ASTC-a-1 cells. Proceedings of the SPIE, Volume 7565, pp. 75650I-75650I-7 (2010),DOI: 10.1117/12.839551 (EI收录) 6 Wei-wei Zhang, Zhi-ping Wang,Tong-sheng Chen*.Bax is not involved in the resveratrol-induced apoptosis in human lung adenocarcinoma cells. Proceedings of the SPIE, Volume 7565, pp. 75650K-75650K-7 (2010),DOI: 10.1117/12.839631. (EI收录) 7 Ying-Ying Lu,Tong-Sheng Chen*,Jun-Le Qu. Bax translocation into mitochondria during dihydroartemisinin(DHA)-induced apoptosis in human lung adenocarcinoma cells. Proc. of SPIE 2009,7178, 71780J 8 Wen-liang Pan,Tong-sheng Chen*,Jun-Le Qu. Emission spectral analysis of caspase-3 activation during artesunate-induced apoptosis of human lung adenocarcinoma cell. Proc. of SPIE 2009,7178, 71780I 9 Qingrui Sun,Tongsheng Chen*.Reactive oxygen species (ROS) is not a promotor of taxol-induced cytoplasmic vacuolization. Proc. of SPIE 2009,7178, 71780K 10 Tong-sheng Chen *, Lei Sun, Huiying Wang, Longxiang Wang.Fluorescence Imaging Analysis of Taxol-Induced Cell Death with Cell Swelling and Cytoplasmic Vacuolization.Proceedings of the SPIE 2008, 6857, 68570L-68570L-8 (2008) (EI收录). 11 Lei Sun,Tongsheng Chen*, Longxiang Wang, Huiying Wang.Analysis of caspase3 activation in ChanSu-induced Apoptosis of ASTC-a-1 Cells by Fluorescence Techniques.Proceedings of the SPIE 2008, 6857, 68570I-68570I-8 (2008) (EI收录). 12 Huiying Wang,Tongsheng Chen*,Lei Sun.Analysis of caspase-3 activation in mitomycin C-induced ASTC-a-1 cell apoptosis using acceptor photobleaching techniques.Proceedings of the SPIE 2008, 6857, pp. 68570J-68570J-7 (2008) (EI收录). 13 Tongsheng Chen, et al. Dynamic imaging of interaction between proteins 14-3-3and Bid in living cells, SPIE, 2006, 6087:60870L (EI收录) 14 Tongsheng Chen, et al. Real-time single cell analysis of molecular mechanism of apoptosis and preliforaion using FRET technique, SPIE, 2006, 6047:604736 (EI收录) 15 Tongsheng Chen, Da Xing. Application of the FRET for Monitoring the Dynamics of Caspase-3 Activation during Apoptosis in Living Cells. SPIE, 2005, 5630: 368(EI收录) 16 Tongsheng Chen, Da Xing. Application of the FRET for Monitoring the Dynamics of Caspase-3 Activation during Apoptosis in Living Cells. SPIE, 2004, 5630:368 (EI收录) 17 Tongsheng Chen, Shaoqun Zeng, Qingming Luo et al., High-Order Photobleaching in Two-Photon Excitation FluorescenceMicroscopy Inside Live Cell. SPIE. 2003 . 4967: 63-67(EI收录) 18 Tongsheng Chen, Yafeng Liu, Shaoqun Zeng, Qingming Luo. Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy. SPIE, 2003,5254:508-512(EI收录) 19 Tongsheng Chen,Shaoqun Zeng, Qingming Luo. Interaction way of photobleaching produced bythe interaction between excitation photons and fluorophores molecules in excited states. SPIE,2003,4967:185-190(EI收录) 20 Tongsheng Chen,et. al. Data analysis in green fluorescent protein based resonance energy transfer. SPIE. 2000, 4224: 346-350 (EI收录)。 1 Tan Li, Liping Wu, Jiang Zhang, Gaina Xi, Yilin Pang, Xiaoping Wang and Tongsheng Chen*. Hydrothermal Reduction of Polyethylenimine and Polyethylene Glycol Dual-Functionalized NanoGraphene Oxide for high-efficiency Gene Delivery. ACS Appl. Mater. Interfaces 2016, 8, 31311−31320. 2 Tan Li, Hongyu Liu, Gaina Xi, Yiling Pang, Liping Wu, Xiaoping Wang *, Tongsheng Chen*. One step reduction and PEIylation ofPEGylated nanographene oxide for high-efficient chemo-photothermal therapy. J Mater Chem B 2016, 4: 2972 – 2983. 3 Yingyao Quan, Guiqi Qin, Hao Huang, Yuhong Liu, Xiaoping Wang, Tongsheng Chen*. Dominant roles of Fenton reaction in sodium nitroprusside-induced chondrocyte apoptosis. Free Radical Biology & Medicine 2016, 94: 135-144. 4 Mengyan Du, Lili Zhang, Shusen Xie, Tongsheng Chen*. Wide-field microscopic FRET imaging using simultaneous spectral unmixing of excitation and emission spectra. Opt. Express 2016, 24(14), 16037-16051. 5 aina Xi, Xiaoping Wang, Tongsheng Chen*. A reduced graphene oxide-based FRET sensor for highly sensitive detection of matrix metalloproteinase 2. Int J Nanomed 2016, 11:1537–1547 6 Jiang Zhang, Fangrui Lin, Liuying Chai, Lichun Wei, Tongsheng Chen*. IIem-spFRET: Improved Iem-spFRET method for robust FRET measurement. J Biomed Opt 2016, 21(10), 105003 7 Yilin Pang, Guiqi Qin, Liping Wu, Xiaoping Wang, Tongsheng Chen*. Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells. Experimental Cell Research 2016, 347: 251–260 8 Hongyu Liu, Tan Li, Yuhong Liu, Guiqi Qin, Xiaoping Wang, and Tongsheng Chen*. Glucose-reduced Graphene Oxide with Excellent Biocompatibility and Photothermal Efficiency as well as Drug Loading. Nanoscale Res Lett 2016, 11:211. 9 Jiang Zhang, Lili Zhang, Liuying Chai, Fangfang Yang, Mengyan Du, Tongsheng Chen*. Reliable measurement of the FRET sensitized-quenching transition factor for FRET quantification in living cells. Micron 2016, 88:7-15. 10 ZP Wang, Y Li, Q Zhou, YF Wang, TS Chen. Berberine-loaded solid lipid nanoparticles enhance hypoglycemic efficacy on streptozotocin-induced diabetic C57BL/6 mice. Nanomed-Nanotechnol Biol Med 2016, 12(2): 483-484. 11 XL Li and TS Chen. Enhancement and suppression effects of a nanopatterned surface on bacterial adhesion. PHYSICAL REVIEW E 2016(93), 052419. 12 H Huang, YY Quan, XP Wang, TS Chen. Gold Nanoparticles of Diameter 13 nm Induce Apoptosis in Rabbit Articular Chondrocytes. Nanoscale Res Lett 2016, 11:249. 1 Lili Zhang, Guiqi Qin, Liuying Chai, Jiang Zhang, Fangfang Yang, Hongqin Yang, Shusen Xie,Tongsheng Chen*. Spectral wide-field microscopic FRET imaging in live cells.J. Biomed. Opt. 2015,20(8), 086011 2 Guiqi Qin, Hongyu Liu, Liping Wu, Yilin Pang, Chubiao Zhao, Shengnan Wu,Xiaoping Wang,Tongsheng Chen*.Artesunate inducesapoptosis via a ROS-independentBax-mediated intrinsic pathway in HepG2 cells. Exp Cell Res 2015, 336(2):308-317 3 Guiqi Qin, Lili Zhang, Hongyu Liu, Yingyao Quan, Liuying Chai, Shengnan Wu, Xiaoping Wang,Tongsheng Chen*. Dihydroartemisinin induces apoptosis preferentially via a Bim/Bak-mediated intrinsic pathway in hepatocarcinoma cells. Apoptosis 2015, 20:1072–1086. 4 Liuying Chai, Jianwei Zhang, Lili Zhang,Tongsheng Chen*.Miniature fiber optic spectrometer-based quantitative fluorescence resonance energy transfer measurement in single living cells," J. Biomed. Opt. 20(3), 037008 (2015). 5 Bingling Chen, Zhouyi Guo,Tongsheng Chen*.Maximum Entropy Method-based Acceptor Triplet-State Fluorescence Correlation Spectroscopy Analysis for Determination of Donor-Acceptor Distance Distribution: Theory and Simulation. IEEE Photonics Journal 2015,7(3):6900710,DOI 10.1109/JPHOT.2015.2417168 6 Jianwei Zhang, Huali Li, Liuying Chai, Lili Zhang, JunLe Qu,Tongsheng Chen*.Quantitative FRET measurement via unmixing emission spectra with independent cross excitation correction. J Microscopy 2015, 257(2):104–116. 7 Jianwei Zhang, Fangfang Yang, Liuying Chai, Lili Zhang, Junle Qu,Tongsheng Chen*.Spectral measurement of acceptor-to-donor extinction coefficient ratio in living cells. Micron 68 (2015) 98–106. 8 Zhiping Wang, Junbiao Wu, Qun Zhou, Yifei Wang, andTongsheng Chen. Berberine Nanosuspension Enhances Hypoglycemic Efficacy on Streptozotocin Induced Diabetic C57BL/6 Mice. Evidence-Based Complementary and Alternative Medicine 2015, 239749. 1 陈同生,于怀娜。一种FRET效率定量检测方法。国家发明专利(2014)专利号:ZL20111032899.8 2 Jingqin Chen,Hongyu Liu, Chubiao Zhao, Guiqi Qin, Gaina Xi, Tan Li,Xiaoping Wang,andTongsheng Chen*.One-step Reduction and PEGylation of Graphene Oxide for Photothermally Controlled Drug Delivery. Biomaterials2014, 35:4986-4995,DOI 10.1016/j.biomaterials.2014.02.032. 3 Lili Zhang, Huaina Yu, Jianwei Zhang, Xiaoping Wang,Tongsheng Chen*.Binomial distribution-based quantitative measurement of multiple-acceptors fluorescence resonance energy transfer by partially photobleaching acceptor.APPLIED PHYSICS LETTERS104, 243706 (2014). 4 Chubiao Zhao, Guiqi Qin, Weijie Gao,Tongsheng Chen*. Dihydroartemisinin more intensely induces apoptosis in gemcitabine-resistant A549 cells via enhancing Bax-mediated intrinsic pathway. Cell Signal 2014,26(10): 2223-2233. DOI 10.1016/j.cellsig.2014.07.001 5 Chubiao Zhao, Weijie Gao,Tongsheng Chen*.Synergistic induction of apoptosis in A549 cells by dihydroartemisinin and gemcitabine. Apoptosis (2014) 19:668–681, DOI: 10.1007/s10495-013-0953-0 6 Jingqing Chen, Xiaoping Wang,Tongsheng Chen*.Facile and green reduction of covalently PEGylated nano-graphene oxide via a “water-only” route for high-efficiency photothermal therapy. Nanoscale Res Lett. 2014,9:86,DOI: 10.1186/1556-276X-9-86. 7 Qian Liang, Xiao-ping Wang,Tong-sheng Chen.Resveratrol protects rabbit articular chondrocyte against sodium nitroprusside-induced apoptosis via scavenging ROS.Apoptosis 2014,2014,19 (9):1354-1363.DOI 10.1007/s10495-014-1012-1. 8 Hua Jin, Qian Liang,Tongsheng Chen,XiaoPing Wang. Resveratrol protects chondrocytes from apoptosis via altering the ultrastructural and biomechanical properties: an AFM study. PLOS ONE 2014, 9(3): e91611, DOI 10.1371/journal.pone.0091611. 9 Weijie Gao, Fenglian Xiao, Xiaoping Wang, Tongsheng Chen*. Artemisinin induces A549 cell apoptosis dominantly via a reactive oxygen species-mediated amplification activation loop among caspase-9, -8 and -3. Apoptosis 2013,18(10): 1201-1213 10 Jing Qi, Yonghong Shao, Lixin Liu, Kaige Wang,Tongsheng Chen*, Junle Qu,* and Hanben Niu.Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector. Optical Letter 2013, 38(9):1697-1699 11 Tongsheng Chen*,Min Chen, Jingqin Chen. Dihydroartemisinin induces apoptosis and sensitizes cells to low-dose ionizing radiation largely by a ROS-mediated extrinsic apoptosis pathway in A549 cells. Plos One 2013, 8(3): e59827. DOI: 10.1371/journal.pone.0059827 12 Huaina Yu, Jianwei Zhang, Huali Li, andTongsheng Chen*. Ma-PbFRET: Multiple Acceptors FRET Measurement Based on PartialAcceptorPhotobleaching. Microscopy and Microanalysis2013,19(1):171-179,. 13 Caiping Zhuang, Xiaoping Wang, andTongsheng Chen*.H2o2 Induces Apoptosis Of Rabbit Chondrocytes Via Both The Extrinsic and The Caspase-Independent Intrinsic Pathways. Journal of Innovative Optical Health Sciences, 2013, 6( 3): 1350022 14 YingYao Quan, Chaoyang Wang, XiaoPing Wang,Tongsheng Chen.Taxol induces cell death with cytoplasm vacuolization in paraptosis-like but not oncosis fashion in ASTC-a-1 cells.Journal of Innovative Optical Health Sciences,2013 15 Huali Li, Huaina Yu,Tongsheng Chen*.Quantitative measurement of FRET efficiencyfree from spectral bleed-throughin living cells. Microsc Microanal 2012,18: 1021–1029. 16 Huaina Yu, Jianwei Zhang, Huali, Li Junle Qu,Tongsheng Chen*. An empirical quantitative FRET method for multiple acceptors based on partial acceptor photobleaching. Appl Phys Letter, 2012,100, 253701 17 WeiWei Zhang, XiaoPing Wang,Tongsheng Chen*. Resveratrol induces apoptosis via a Bak-mediated intrinsic pathway in human lung adenocarcinoma cells. Cellular Signaling 2012,24(5):1037-1046 18 Chenjun Zhou, Wenliang Pan,Tongsheng Chen*. Artesunate induces apoptosis largely via a Bak-mediated caspases-independent intrinsic pathway in human lung adenocarcinoma cells. J Cell Physiol 2012,227(12):3778-3786 19 Fenglian Xiao, Weijie Gao, Xiaoping Wang,Tongsheng Chen*. Amplification activation loop between caspase-8 and -9 dominates artemisinin-induced apoptosis of ASTC-a-1 cells. Apoptosis 2012,17(6): 600-611. 20 Chaoyang Wang,Tongsheng Chen*. Intratumoral injection of taxol in vivo suppresses A549 tumor showing cytoplasmic vacuolization. J Cell Biochem 2012, 113:1397–1406 21 Min Chen,Tong-sheng Chen*, Ying-ying Lu, Cheng-yi Liu, Jun-le Qu. Dihydroarteminsin (DHA) induced apoptosis is not dependent on the translocation of Bim to the endoplasmic reticulum in human lung adenocarcinoma cells. Pathol. Oncol. Res. (2012) 18:809–816 22 Haiyang Li, Xiaoping Wang,Tongsheng Chen*.P38 Inhibitor SB203580 Sensitizes the Resveratrol-Induced Apoptosis in Human Lung Adenocarcinoma (A549) Cells.Journal of Biochemical and Molecular Toxicology2012, 26(7): 251 23 XiaoPing Wang,JianWei Zhang, Huai-na Yu,Tong-sheng Chen*.Quantitatively monitoringthe caspase-3 activationin single live cells using partial acceptor photobleachingFRET method.Journal of Innovative Optical Health Sciences(JHOS) 2012,5(3),1250015 24 陈同生,于怀娜.一种基于受体部分光漂白和供体-受体交替激发的FRET效率定量检测方法.国家发明专利,专利申请号:20110238171.4 25 Lei Sun,Tongsheng Chen*, Xiaoping Wang, Yun Chen, Xunbin Wei. Bufalin Induces Reactive Oxygen Species (ROS)-Dependent Bax Translocation and apoptosis in ASTC-a-1 Cells. EVID-BASED COMPL ALT 2011, 249090 26 Wenjing Guo, Lei Qian, Jing Zhang, Wei Zhang,Tongsheng Chen*, and Jie Zhao. SIRT1 overexpression in neurons promotes neurite outgrowth and cell survival with reduced mTOR signaling. J Neurosci Res 2011,89(11):1723-1736 27 Weiwei Zhang, Xiaoping Wang,Tongsheng Chen*.Resveratrol induces mitochondria-mediated AIF- and to a lesser extent caspase-9-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells. Molecular and Cellular Biochemistry 2011,354:29–37DOI: 10.1007/s11010-011-0802-9 28 WeiWei Zhang, Zhi-Ping Wang,Tongsheng Chen*. Curcumol induces apoptosis via caspases-independent mitochondrial pathway in huaman lung adenocarcinoma ASTC-a-1 cells. MEDICAL ONCOLOGY 2011, 28(1):307-314 29 Fenglian Xiao,Tongsheng Chen*,Chengyi Liu, Xiaoping Wang. Artemisinin induces caspase-8/9-mediated and Bax/Bak-independent apoptosis in human lung adenocarcinoma (ASTC-a-1) cells. Journal of X-Ray Science and Technology(JXST) 2011,19(4): 545-555. 30 Li-hui Wang, Jin Xiang, Min Yan, Yan Zhang, Yan Zhao, Cai-feng Yue, Jie Xu, Fei-meng Zheng, Jin-na Chen, Zhuang Kang,Tong-sheng Chen, Da Xing, and Quentin Liu. Mitotic kinase Aurora-A induces mammary cell migration and breast cancer metastasis through up-regulating cofilin activity. Cancer Research, 2010,70(22):9118-9128 31 陈同生,王龙祥.基于光漂白的荧光共振能量效率的定量测量方法.国家发明专利,专利号:ZL200910039454.9 32 Qing Rui Sun,Tong Sheng Chen*,Xiao Ping Wang, Xunbin Wei. Taxol induces paraptosis independent of protein synthesis and MAPK pathway. J Cell Physiol 2010, 222( 2) : 421-432 33 Ying-Ying Lu,Tong-Sheng Chen*, Xiao-Ping Wang, Jun-Le Qu. The JNK inhibitor SP600125 enhances dihydroartemisinin-induced apoptosis by accelerating Bax translocation into mitochondria in human lung adenocarcinoma cells. FEBS Letter, 584 (2010):4019–4026(SCI IF 3.6) 34 Ying-Ying Lu,Tong-Sheng Chen*, Xiao-Ping Wang , Li Li. Single cell analysis of dihydroartemisinin(DHA)-induced apoptosis through reactive oxygen species (ROS)-mediated caspase-8 activation and mitochondrial pathway in ASTC-a-1 cells using fluorescence imaging techniques.J. Biomed. Opt., 2010,15(4): 046028-1-16 35 Min Chen, Ying-ying Lu,Tong-sheng Chen*,Jun-le Qu. Confocal Imaging of Bim Translocation to ER during DHA-Induced ASTC-a-1 Cell Apoptosis. Chinese Optical Letter, 2010, 8(10):950-953 36 Huai-na Yu,Tong-sheng Chen*, Jun-le Q. Improving FRET efficiency measurement in confocal microscopy imaging. Chinese Optical Letter, 2010, 8(10):947-949 37 Longxiang Wang,Tongsheng Chen*, Junle Qu, Xunbin Wei. Photobleaching-Based Quantitative Analysis of Fluorescence Resonance Energy Transfer Inside Single Living Cell. J Fluorescence 2010, 20(1):27-35. 38 Wenjing Guo,Tongsheng Chen*, Xiaoping Wang, Rong Chen. Taxol induces concentration-dependent cell death form in ASTC-a-1 cells.Journal of X-Ray Science and Technology (JXST) , 18 (2010) 293–308 39 Longxiang Wang,Tongsheng Chen*,JunLe Qu et al. Quantitative analysis of caspase-3 activation by fitting fluorescence emission spectra in living cells. Micron 2009, 40(12): 811–820 40 Ying-Ying Lu,Tong-Sheng Chen*,Jun-Le Qu, Wen-Liang Pan, Lei Sun, Xunbin Wei.Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma cells. J Biomed Sci2009,16(1):16-. doi:10.1186/1423-0127-16-16 41 Wenliang Pan, Junle Qu,Tongsheng Chen*,Lei Sun, Jing Qi.FLIM and emission spectral analysis of caspase-3 activation inside single living cell during anticancer drug-induced cell death. Eur Biophy J 2009,38:447–456 42 Longxiang Wang, Xiangbo Yang,Tongsheng Chen.Second harmonic generation in generalized Thue–Morse ferroelectric superlattices. Physica B: Condensed Matter,2009, 404: 3425-3430 43 Xiaoping Wang, Dongmei He, Jiye Cai,Tongsheng Chen, Feiyan Zou, Yalan Li ,Yangzhe Wu, Zheng W. Chen, Yong Chen. WGA-QD probe-based AFM detects WGA-binding sites on cell surface and WGA-induced rigidity alternation. Biochem. Biophys. Res. Commun.2009379:335–340 44 Tong-sheng Chen*, Xiao-ping Wang, Lei Sun, Long-xiang Wang, Da Xing,Martin Mok.Taxol induces caspase-independent cytoplasmic vacuolization and cell death through endoplasmic reticulum (ER) swelling in ASTC-a-1 cell. Cancer Letters 270 (2008):164-172. 45 Lei Liu, Da Xing, Wei R. Chen,Tongsheng Chen, Yihui Pei, and Xuejuan Gao.Calpain-mediated pathway dominates cisplatin-induced apoptosis as determined by real-time single cell analysis. International Journal of Cancer. 122, 2210-2222 (2008).. 46 Xiao-Ping Wang,Tong-Sheng Chen*, Lei Sun, Ji-Ye Cai, Ming-Qian Wu,Martin Mok.Live Morphological Analysis of Taxol-Induced Cytoplasmic Vacuoliazation in human lung adenocarcinoma cells. Micron 2008,39:1216-1221. 47 Lei Sun, Xiao-Ping Wang,Tong-Sheng Chen*.Involvement of Bax and caspase-3 activation in Chan-Su-induced ASTC-a-1 cell apoptosis. Journal of X-ray Science and Technology (JXST) (2008), 16:131–142. 48 CHEN Tong-sheng*, WANG Xiao-ping, Sun Lei, WANG Hui-ying, WANG Long-xiang.Caspase-3 is Involved in the Chan-Su(CS)-induced Human LungAdenocarcinoma(ASTC-a-1) Cell Apoptosis. Progress in Biochemistry and Biophysics, 2008, 35(1):83-90. 49 CHEN Tong-sheng*, WANG Xiao-ping, WANG Zhi-ping, WANG Long-xiang1, WANG Hui-ying. Fluorescence Emission Analysis of Caspase-3 Activation Induced by Xiao-Ai-Ping (XAP) Inside Living Human Lung Adenocarcinoma Cells. Spec & Spec Anal.2008,28(6): 1327-1331. 50 CHEN Tong-sheng*, WANG Xiao-ping, SUN Lei, WANG Hui-ying, WANG Long-xiang. Fluorescence Analysis of Taxol-Induced Paraptosis-Like Independent of Caspase-3 Activation. Spec & Spec Anal2008,28(11):2623-2627. 51 Xiao-ping Wang,Tongsheng Chen*, Longxiang Wang, Lei Sun. Live Imaging of Xiao-Ai-Ping-induced Cell Death in Human Lung Adenocarcinoma Cells. Journal of Innovation in Optical Health Science (JIOHS) 2008, 1(1): 151-156. 52 Tongsheng Chen, Jinjun Wang, Da Xing and Wei R. Chen.Spatio-temporal dynamic analysis of Bid activation and apoptosis induced by alkaline condition in human lung adenocarcinoma cell. Cell physiology and biochemistry.Cell Physiol Biochem,2007,20(8):569-578 53 Yihui pei, Da xing, Xuejuan gao,Tongsheng chen, Lei liu, Real-time Monitoring Full length Bid Interacting with Bax during TNF-α-induced Apoptosis, 2007, Apoptosis, 12:1681-1690 54 Yin-Yuan WU, Da XING, Lei LIU,Tong-sheng CHEN,and Wei CHEN. FRET Analysis of Bid Activation in Living Cells during UV-induced Apoptosis. Acta Biochimica et Bophysica,2007,39(1):37-45 55 MiaoJuan Chen, Da Xing,Tongsheng Chen, and Lan Zhang. JNK phosphorylation of BimL Induces Bax activation during UV irradiation-induced apoptosis. BBRC, 2007, 352(2), 559-565 56 Shengnan Wu, Da Xing, Fang Wang,Tongsheng Chen, Wei R. 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