个人简介

College of Sciences, GA Tech, Faculty Mentoring Award, 2012 American Chemical Society (ACS) Fellow, 2010 American Association for the Advancement of Science (AAAS) Fellow, 2009 WISE (Women in Science and Engineering) Lectureship. University of Minnesota, 2001 Edna Roe Lectureship. International Union of Photobiology, 2000 National Honorary Member. Iota Sigma Pi, 1999 Career Advancement Award. National Science Foundation, 1997 Bush Sabbatical Award. University of Minnesota, 1997 Bush Foundation Faculty Development Program. University of Minnesota, 1992-1993 McKnight-Land Grant Professor, University of Minnesota, 1990-1993 Graduate School, University of Minnesota, Faculty Summer Research Fellowship. 1989 Public Health Service Award, National Institutes of Health, October, 1985 - October, 1988 McKnight Postdoctoral Fellow, Michigan State University, March, 1985 - September, 1985 Graduate Opportunity Fellowship, University of California, September, 1982 - June, 1983 Senior Award for Excellence in Chemistry, Oberlin College, 1978 NSF Summer Fellow, Jackson Laboratory, Maine, Summer, 1977 Carborundum Scholarship, Carborundum, Inc., September, 1974 - May, 1978 Phi Beta Kappa Sigma Xi

研究领域

Biochemistry/Biomolecular Structure and Function/Chemical Biology Energy and Sustainability/Molecular Biophysics Physical/Chemistry Spectroscopy and Dynamics

Research in my group is focused on how the dynamic and responsive protein matrix facilitates biological catalysis. We use a wide range of high resolution spectroscopic, biochemical, and structural techniques to describe the reaction coordinate, which reveals the motion of the protein in space and time. We test the design principles, which we uncover, by building biomimetic models. Photosynthetic water oxidation and solar energy conversion. Photosystem II uses light energy to oxidize water, producing molecular oxygen. The reaction is catalyzed at a Mn4CaO5 cluster using a photo-generated tyrosyl radical. We are studying the catalytic steps of this reaction using resonance Raman, FT-IR, and EPR spectroscopies. We have developed a method to incorporate unnatural amino acid residues into the oxygen-evolving center. Noncanonical amino acid residues provide unique spectroscopic tools. Elucidation of the water oxidizing mechanism will contribute to new developments in solar energy conversion. Biomimetic peptide models. Biological proton coupled electron transfer reactions are subjected to exquisite control over direction and kinetics; these mechanisms are not yet understood. We seek to assemble nanoscale, peptide-based devices, which can carry out similar reactions. These maquettes (or models) will provide water-soluble, stable redox agents with tunable redox potentials, kinetics, and directional control of charge transfer. These efforts will produce prototypes, which will generate new insights into important biological reactions, such as photosynthesis and DNA synthesis. We have succeeded in engineered a robust photosystem II-inspired scaffold, which exhibits a proton coupled electron transfer reaction. Proton coupled electron transfer and DNA synthesis. Ribonucleotide reductase catalyzes the production of deoxyribonucleotides in all cells. Ribonucleotide reductase is the target of anti-cancer therapeutics. This protein uses a tyrosyl radical-diferric cofactor as a radical initiator. We seek to understand how the oxidizing power of the intermediary tyrosyl free radical is controlled. This research project, using reaction-induced FT-IR spectroscopy, gives a picture of the movements of the electron and proton and describes the protein conformational landscape associated with the transfer. Elucidation of these mechanisms will contribute to the design of more effective cancer therapeutic agents.

近期论文

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Reaction dynamics and proton coupled electron transfer: studies of tyrosine-based charge transfer in natural and biomimetic systems. Bridgette A Barry. Biochimica Biophysica Acta-Bioenergetics. 2015, 1847, 46-54. The first site-specific incorporation of a non-canonical amino acid into the photosynthetic oxygen-evolving complex. Adam R. Offenbacher, Cynthia V. Pagba, Brandon C. Polander, Udita Brahmachari, and Bridgette A. Barry. ACS Chemical Biology (Letter) . 2014, 9, 891–896. Redox-dependent structural coupling between the α2 and β2 subunits in E. coli ribonucleotide reductase. Adam R. Offenbacher, R. Atlee Watson, Cynthia V. Pagba, and Bridgette A. Barry. Journal of Physical Chemistry B. 2014, 118, 2993–3004. Free radicals under control. Bridgette A. Barry. Nature Chemistry . 2014, 6, 376-377. An intrinsically disordered photosystem II subunit, PsbO, provides a structural template and a sensor of the hydrogen bonding network in photosynthetic water oxidation. Adam R. Offenbacher, Brandon C. Polander, and Bridgette A. Barry. Journal of Biological Chemistry. 2013, 288, 29056-29068. Redox-linked changes to the hydrogen bonding network of ribonucleotide reductase β2. Adam R. Offenbacher, Ellen Minnihan, JoAnne Stubbe, and Bridgette A. Barry. Journal of the American Chemical Society (Communication). 2013, 135, 6380–6383. Redox-linked conformational control of proton coupled electron transfer: Y122 in the ribonucleotide reductase β2 subunit. Adam R. Offenbacher, Lori A. Burns, C. David Sherrill, and Bridgette A. Barry. Journal of Physical Chemistry B. 2013, 117, 8457-8468. Calcium, strontium, and protein dynamics during the S2 to S3 transition in the photosynthetic oxygen-evolving cycle. Brandon C. Polander and Bridgette A. Barry. Journal of Physical Chemistry Letters . 2013, 4, 3356–3362. Detection of an intermediary, protonated water cluster in photosynthetic oxygen evolution. Brandon C. Polander and Bridgette A. Barry. Proceedings of the National Academy of Sciences. 2013, 110, 10634-10639. Calcium and the hydrogen-bonding network in the photosynthetic oxygen-evolving complex. Brandon C. Polander and Bridgette A. Barry. Journal of Physical Chemistry Letters. 2013, 4, 786-791. Redox control and hydrogen bonding networks: Proton-coupled electron transfer reactions and tyrosine Z in the photosynthetic oxygen-evolving complex. James Keough, Ashley Zuniga, David L. Jenson and Bridgette A. Barry. Journal of Physical Chemistry. 2013, B 117, 1296−1307. Two-dimensional crystallization of membrane proteins by detergent removal through dialysis. Matthew J. Johnson, Tina M. Dreaden Kasson, Laura Y. Kim, Frederik Rudolph, Bridgette A. Barry, and Ingeborg Schmidt-Krey. Methods in Molecular Biology. 2013, 955, 31-58. Structure-function insights on membrane and soluble proteins revealed by electron crystallography. Tina M. Dreaden Kasson, B. Devaragan, Bridgette A. Barry, and Ingeborg Schmidt-Krey. Methods in Molecular Biology. 2013, 955, 519-526. Screening for two-dimensional crystals by transmission electron microscopy of negatively stained samples. Tina M. Dreaden Kasson, Maureen Metcalf, Laura Y. Kim, Matthew C. Johnson, Bridgette A. Barry, and Ingeborg Schmidt-Krey. Methods in Molecular Biology. 2013, 955, 73-101. Proton coupled electron transfer and redox active tyrosines: Structure and function of the tyrosyl radicals in Ribonucleotide Reductase and Photosystem II. Barry, B., Chen J., Keogh, J., Jenson, D., Offenbacher, A., Pagba, C. Journal of Physical Chemistry Letters (Perspective). 2012, 3, 543-554. Light-induced oxidative stress, N-formylkynurenine, and oxygenic photosynthesis. Dreaden Kasson TM, Rexroth S, Barry BA. PLos One 7. 2012, 7, e42220. Redox-induced conformational switching in a Photosystem II-inspired peptide. Pagba C., Barry, B. Journal of Physical Chemistry B. 2012, 116, 10590-10599. A hydrogen bonding network plays a catalytic role in photosynthetic oxygen evolution. Polander BC, Barry BA. Proceedings of the National Academy of Sciences. 2012, 109, 6112-6117. Reactive oxygen and oxidative stress: N-formyl kynurenine in photosystem II and non-photosynthetic proteins. Tina M. Dreaden Kasson and Bridgette A. Barry. Photosynthesis Research (Invited review). 2012, 114, 97-110. Perturbations of aromatic amino acids are associated with iron cluster assembly in ribonucleotide reductase. Adam Offenbacher, Jun Chen, and Bridgette A. Barry. Journal of the American Chemical Society. 2011, 133, 6978-88.