Smith, Andrew - RMIT University - School of Science, Applied Sciences

研究领域

Structure/function relationships and mechanisms of metalloenzymes with particular emphasis on haem systems and peroxidases, particularly those that play a role in the degradation of lignin, the recalcitrant biopolymer that gives wood its strength. Degradation of lignin is known to be rate limiting in the carbon cycle and clean technologies that would help us unlock lignin and hence cellulose as renewable sources of chemicals and fuels may be of great importance in the future. Professor Smith’s research group is exploring the relatonships between globins, peroxidases and cytochromes P450 using horseradish peroxidase and lignin peroxidase as our main model systems.

近期论文

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Smith, A.T., Doyle, W.A., Dorlet, P. and Ivancich, A. (2009) Spectroscopic evidence for an engineered catalytically-active Trp radical that creates the unique reactivity of lignin peroxidase. PNAS 106, 1684-1689. Wiseman, B., Colin, J., Smith, A.T., Ivancich, A. and Loewen P.C. (2009) Mechanistic insight into the initiation step of the reaction of Burkholderia pseudomallei catalase-peroxidase with peroxyacetic acid. Journal of Biological Inorganic Chemistry, 14, 5, 801-811. Ruiz-Dueñas, F.J. , Morales, M., Mate, M.J. Romero, A., Martínez, M.J., Smith, A.T. and Martínez, A.T. (2008) Site-directed mutagenesis of the catalytic tryptophan environment in Pleurotus eryngii versatile peroxidase. Biochemistry, 47, 1685-1695. Smith A.T and Ngo E. (2007) Novel peroxidases and uses, Patent number: WO/2007/020428 PCT/GB2006/003045. Smith A.T. and Doyle W.A. (2006) Engineered peroxidases with veratryl alcohol oxidase activity, Patent number: WO/2006/114616, PCT/GB2006/001515. Howes, B. D., Brissett, N.C., Doyle, W.A, Smith, A.T & Smulevich, G. Spectroscopic and Kinetic Properties of the Horseradish Peroxidase Mutant T171S: Evidence for Selective Effects on the Reduced State of the Enzyme (2005) FEBS 272, 5514. O'Brien, A.M., Smith, A.T., O'Faigain, C. (2003) Effects of phthalic anhydride modification on horseradish peroxidase stability and activity. Biotech & Bioengineering 81, 233-240. Meno, K., Jennings, S., Smith, A.T., Henricksen, A, & Gajhede, M (2002) Structural analysis of the two horseradish peroxidase catalytic residue variants H42E and R38S:H42E: Implications for the catalytic cycle. Acta Cry. Sec. D - Biol. Cry. 58, 1803-12. Hiner A.N.P., Hermandez-Ruiz, J., Rodrigues Lopez, J.N., Garcia-Canovas, F., Brissett, N.C., Smith A.T., Arnao M.B., & Acosta, M. (2002) Reactions of the class II peroxidases, lignin peroxidase and Arthromyces ramosus peroxidase, with hydrogen peroxide - Catalase-like activity, compound III formation and enzyme inactiviation. J. Biol. Chem. 277, 26879-26885. Berglund, G.I., Carlsson, G.H., Smith, A.T. Szoke, H., Henriksen, A., & Hajdu, J. (2002) The catalytic pathway of horseradish peroxidase at high resolution. Nature 417, 463-468. Heering, H.A. Smith, A.T. & Smulevich. G. (2002) Spectroscopic characterisation of mutations at the Phe 41 position in the distal heme pocket of horseradish peroxidase C: Structural and functional consequences. Biochem. J. 363, 571-579. Adams, B., Lowe, D.J., Smith, A.T., Scazzocchio, C., Demais, S & Bray, R.C. (2002). Expression of Drosophila melanogaster xanthine dehydrogenase in Aspergillus nidulans and properties of the recombinant wild-type enzyme. Biochem. J. 362, 223-229. Perez-Boada, M., Doyle, W.A., Ruiz-Duenas, F.J., Martinez, M.J. Martinez, A.T & Smith A.T. (2002) Folding optimisation of Pleurotus eryngii versatile peroxidase expressed in E.Coli. Enz. Microbial. Tech. 30, 518-524. Heering H.A., Jansen M.A.K., Schneider-Belhaddad F., Smith A.T, Thorneley RNF, & Smulevich. G. (2001) Spectroscopic characterization of two atypical class III peroxidases from tea. J. Inorg. Biochem 86, (1): 256. Smith A.T. & Schneider-Belhaddad F. (2001) Mimicking the active site of chloroperoxidase: characterisation of the HRP distal pocket mutants R38H/H42E, R38H:F41A:H42E and R38A:F41H:H42E. J. Inorg. Biochem 86, (1): 99.