Griffin, Timothy J - University of Minnesota Twin Cities

个人简介

Director, Center for Mass Spectrometry and Proteomics Ph.D., University of Wisconsin, 1999 Postdoctoral Research, University of Washington and Institute for Systems Biology, 1999-2003

研究领域

Work in our group involves the development and application of mass spectrometry-based tools to study proteins and proteomes. The goal of this work is to provide the necessary tools to enable the system-wide characterization of proteins expressed within a cell, tissue, biological fluid or organism, in order to better understand basic mechanisms of biological function and disease. These tools must be capable of measuring the many properties of proteins that collectively determine their function. These properties include protein abundance, sub-cellular localization, post-translational modifications, associations in non-covalent complexes and biochemical activity. Mass spectrometry provides a highly powerful tool that can aid in measuring these various protein properties, in a high-throughput manner. The development and application of these tools is highly interdisciplinary in nature, integrating front-end molecular biology and biochemical methods, protein and peptide chemistry, analytical separations, instrumental analysis, and back-end computation and bioinformatics for data analysis and biological interpretation.

近期论文

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Boekel,J., Chilton, J.M., Cooke, I.R., Horvatovich, P.L., Jagtap P.D, Käll, L., Lehtiö, J., Lukasse, P., Moerland, P.D. and Griffin, T.J. (2015) Multi-omic data analysis using Galaxy, Nature Biotechnology, 33: 137–139 PMID: 25658277 Jagtap PD, Johnson JE, Onsongo G, Sadler FW, Murray K, Wang Y, Sheynkman GM, Bandhakavi S, Smith LM, Griffin TJ. (2014) Flexible and accessible workflows for improved proteogenomic analysis using the Galaxy framework. J Proteome Res, 13: 5898-908 PMID: 25301683 PMCID: PMC4261978 Sheynkman GM, Johnson JE, Jagtap PD, Shortreed MR, Onsongo G, Frey BL, Griffin TJ, Smith LM. (2014) Using Galaxy-P to leverage RNA-Seq for the discovery of novel protein variations, BMC Genomics 15: 703. PMID: 25149441; PMCID: PMC4158061 Yang, Y., Rhodus, N.L., Ondrey, F.G., Wuertz, B.R.K., Chen, X., Zhu, Y., and Griffin, T.J. (2014) Quantitative proteomic analysis of oral brush biopsies identifies secretory leukocyte protease inhibitor as a promising, mechanism-based oral cancer biomarker PLoSONE, 9, e95389. PMID: 24748380; PMCID: PMC3991667 Van Riper, S.K., de Jong, E.P., Higgins, L., Carlis, J.V. and Griffin, T.J. (2014) Improved intensity-based label free quantification via proximity-based intensity normalization (PIN) J Proteome Res, 13:1281-92. PMID: 22950739 PMCID; PMCID: PMC3487405 Jagtap P., Goslinga J., Kooren J.A., McGowan T., Wroblewski M.S., Seymour S.L., Griffin T.J. (2013) A two-step database search method improves sensitivity in peptide sequence matches for metaproteomics and proteogenomics studies. Proteomics, 13: 1352-7. PMID: 23412978; PMCID: PMC3633484 Jagtap, P., McGowan, T., Bandhakavi, S., Tu, Z. J., Seymour, S., Griffin, T.J. and Rudney, J.D. (2012) Deep metaproteomic analysis of human salivary supernatant. Proteomics 12: 992-1001. PMID: 22522805; PMCID: PMC3517020 de Jong E, Griffin T.J. (2012) Online nanoscale ERLIC-MS outperforms RPLC-MS for shotgun proteomics in complex mixtures. J Proteome Res, 11, 5059-64. PMID: 22950739; PMCID: PMC3487405 Kim, Y.M., Stone, M., Hwang, T.H., Kim, Y.G., Dunlevy, J.R., Griffin, T.J. and Kim, D.H. (2012) SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 signaling. Molecular Cell 46. (PDF)