个人简介

B.A., 1981, Yale Ph.D., 1989, University of California, San Diego

研究领域

Biophysics

We study how the nervous system controls behavior by analyzing the neural networks that control chemotaxis and thermotaxis, simple forms of spatial orientation behavior, in the nematode worm Caenorhabditis elegans. We investigate how these networks function using a combination of experimental and theoretical approaches. We track the movements of normal and mutant worms at high spatial and temporal resolution to determine the behavioral strategies underlying spatial orientation in C. elegans. Individual neurons in the networks are killed with a laser microbeam to identify their role in behavior. Patch-clamp recordings are made from normal and mutant animals to determine how the electrical properties of neurons influence network function. We also make optical recordings in freely moving animals to correlate neuronal activity patterns and behavior; these experiments are facilitated by microfluidic devices to control the worm's local environment. Data generated by the experimental approaches are synthesized in theoretical models of the spatial orientation networks. Predictions from the models are tested experimentally and the results are used to improve our theoretical understanding of the function of biological networks. These results provide new insights into the cellular and molecular mechanisms of information processing underlying animal behavior.

近期论文

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Connolly AA, Osterberg V, Christensen S, Price M, Lu C, Chicas-Cruz K, Lockery S, Mains PE, Bowerman B. C. elegans oocyte meiotic spindle pole assembly requires microtubule severing and the calponin homology domain protein ASPM-1. Mol Biol Cell. 2014 Feb 19. [Epub ahead of print] PubMed PMID: 24554763. Song BM, Faumont S, Lockery S, Avery L. Recognition of familiar food activates feeding via an endocrine serotonin signal in Caenorhabditis elegans. Elife. 2013 Feb 5;2:e00329. doi: 10.7554/eLife.00329. PubMed PMID: 23390589; PubMed Central PMCID: PMC3564447. Faumont S, Lindsay TH, Lockery SR. Neuronal microcircuits for decision making in C. elegans. Curr Opin Neurobiol. 2012 Aug;22(4):580-91. Epub 2012 Jun 12. Review. PubMed PMID: 22699037; PubMed Central PMCID: PMC3593597. Lockery SR, Hulme SE, Roberts WM, Robinson KJ, Laromaine A, Lindsay TH, Whitesides GM, Weeks JC. A microfluidic device for whole-animal drug screening using electrophysiological measures in the nematode C. elegans. Lab Chip. 2012 Jun 21;12(12):2211-20. doi: 10.1039/c2lc00001f. Epub 2012 May 15. PubMed PMID:22588281; PubMed Central PMCID: PMC3372093. Goodman MB, Lindsay TH, Lockery SR, Richmond JE. Electrophysiological methods for Caenorhabditis elegans neurobiology. Methods Cell Biol. 2012;107:409-36. doi:10.1016/B978-0-12-394620-1.00014-X. PubMed PMID: 22226532. McCormick KE, Gaertner BE, Sottile M, Phillips PC, Lockery SR. Microfluidic devices for analysis of spatial orientation behaviors in semi-restrained Caenorhabditis elegans. PLoS One. 2011;6(10):e25710. doi:10.1371/journal.pone.0025710. Epub 2011 Oct 12. PubMed PMID: 22022437; PubMed Central PMCID: PMC3192130. Faumont S, Rondeau G, Thiele TR, Lawton KJ, McCormick KE, Sottile M, Griesbeck O, Heckscher ES, Roberts WM, Doe CQ, Lockery SR. An image-free opto-mechanicalsystem for creating virtual environments and imaging neuronal activity in freely moving Caenorhabditis elegans. PLoS One. 2011;6(9):e24666. doi:10.1371/journal.pone.0024666. Epub 2011 Sep 28. PubMed PMID: 21969859; PubMedCentral PMCID: PMC3182168. Lockery SR. The computational worm: spatial orientation and its neuronal basis in C. elegans. Curr Opin Neurobiol. 2011 Oct;21(5):782-90. doi:10.1016/j.conb.2011.06.009. Epub 2011 Jul 18. Review. PubMed PMID: 21764577. Lindsay TH, Thiele TR, Lockery SR. Optogenetic analysis of synaptic transmission in the central nervous system of the nematode Caenorhabditis elegans. Nat Commun. 2011;2:306. doi: 10.1038/ncomms1304. PubMed PMID: 21556060; PubMed Central PMCID: PMC3935721. Singh K, Chao MY, Somers GA, Komatsu H, Corkins ME, Larkins-Ford J, Tucey T, Dionne HM, Walsh MB, Beaumont EK, Hart DP, Lockery SR, Hart AC. C. elegans Notch signaling regulates adult chemosensory response and larval molting quiescence. Curr Biol. 2011 May 24;21(10):825-34. doi: 10.1016/j.cub.2011.04.010. Epub 2011 May 5. PubMed PMID: 21549604; PubMed Central PMCID: PMC3100419. Goldsmith AD, Sarin S, Lockery S, Hobert O. Developmental control of lateralized neuron size in the nematode Caenorhabditis elegans. Neural Dev. 2010 Dec 1;5:33. doi: 10.1186/1749-8104-5-33. PubMed PMID: 21122110; PubMed Central PMCID: PMC3014911. Izquierdo EJ, Lockery SR. Evolution and analysis of minimal neural circuits for klinotaxis in Caenorhabditis elegans. J Neurosci. 2010 Sep 29;30(39):12908-17. doi: 10.1523/JNEUROSCI.2606-10.2010. PubMed PMID: 20881110; PubMed Central PMCID: PMC3422662. Takayama J, Faumont S, Kunitomo H, Lockery SR, Iino Y. Single-cell transcriptional analysis of taste sensory neuron pair in Caenorhabditis elegans. Nucleic Acids Res. 2010 Jan;38(1):131-42. doi: 10.1093/nar/gkp868. Epub 2009 Oct 29. PubMed PMID: 19875417; PubMed Central PMCID: PMC2800226. Thiele TR, Faumont S, Lockery SR. The neural network for chemotaxis to tastants in Caenorhabditis elegans is specialized for temporal differentiation. J Neurosci. 2009 Sep 23;29(38):11904-11. doi: 10.1523/JNEUROSCI.0594-09.2009. PubMed PMID: 19776276; PubMed Central PMCID: PMC3376908. Ortiz CO, Faumont S, Takayama J, Ahmed HK, Goldsmith AD, Pocock R, McCormick KE, Kunimoto H, Iino Y, Lockery S, Hobert O. Lateralized gustatory behavior of C. elegans is controlled by specific receptor-type guanylyl cyclases. Curr Biol. 2009 Jun 23;19(12):996-1004. doi: 10.1016/j.cub.2009.05.043. Epub 2009 Jun 11. PubMed PMID: 19523832; PubMed Central PMCID: PMC2730525. Lockery SR. Neuroscience: A social hub for worms. Nature. 2009 Apr 30;458(7242):1124-5. doi: 10.1038/4581124a. PubMed PMID: 19407792. Lockery SR, Goodman MB. The quest for action potentials in C. elegans neurons hits a plateau. Nat Neurosci. 2009 Apr;12(4):377-8. doi: 10.1038/nn0409-377. PubMed PMID: 19322241. Lockery SR, Goodman MB, Faumont S. First report of action potentials in a C. elegans neuron is premature. Nat Neurosci. 2009 Apr;12(4):365-6; author reply 366. doi: 10.1038/nn0409-365. PubMed PMID: 19322234. Suzuki H, Thiele TR, Faumont S, Ezcurra M, Lockery SR, Schafer WR. Functional asymmetry in Caenorhabditis elegans taste neurons and its computational role in chemotaxis. Nature. 2008 Jul 3;454(7200):114-7. doi: 10.1038/nature06927. PubMed PMID: 18596810; PubMed Central PMCID: PMC2984562. Frøkjaer-Jensen C, Ailion M, Lockery SR. Ammonium-acetate is sensed by gustatory and olfactory neurons in Caenorhabditis elegans. PLoS One. 2008 Jun 18;3(6):e2467. doi: 10.1371/journal.pone.0002467. PubMed PMID: 18560547; PubMed Central PMCID: PMC2413426.