个人简介

Ph.D., University of Chicago B.A., UC Berkeley

研究领域

Biophysics

The Parthasarathy lab constructs experiments that explore a variety of topics in biophysics, focusing especially on the physical properties of membranes. Recent work has examined, for example, the mechanisms by which intracellular trafficking proteins may sculpt organelle membranes and the peculiar fluid dynamics of lipid bilayers. We also develop imaging and image analysis tools that enable these sorts of studies. See our lab webpage for more information.

近期论文

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Ryan Baker, Michael J. Taormina, Matthew Jemielita, and Raghuveer Parthasarathy, "A combined light sheet fluorescence and differential interference contrast microscope for live imaging of multicellular specimens," J. Microscopy [published online ahead of print] (2015). DOI: 10.1111/jmi.12220 Matthew Jemielita, Michael J Taormina, Adam R Burns, Jennifer S Hampton, Annah S Rolig, Karen Guillemin, Raghuveer Parthasarathy, "Spatial and temporal features of the growth of a bacterial species colonizing the zebrafish gut," mBio 5: e01751-14 (2014). Tristan T. Hormel, Sarah Q. Kurihara, M. Kathleen Brennan, Matthew C. Wozniak, and Raghuveer Parthasarathy, "Measuring Lipid Membrane Viscosity Using Rotational and Translational Probe Diffusion," Phys. Rev. Lett. 112, 188101 (2014). Kyle J. Welch, Isaac Hastings-Hauss, Raghuveer Parthasarathy, and Eric I. Corwin, "Ballistic and diffusive dynamics in a two-dimensional ideal gas of macroscopic chaotic Faraday waves," Phys. Rev. E 89: 042143 (2014). Andrew F. Loftus, Sigrid Noreng, Vivian L. Hsieh, and Raghuveer Parthasarathy, "Robust Measurement of Membrane Bending Moduli Using Light Sheet Fluorescence Imaging of Vesicle Fluctuations," Langmuir 29: 14588-14594 (2013). Alexander R. Small and Raghuveer Parthasarathy, "Superresolution Localization Methods," Annual Reviews of Physical Chemistry 65: 107-125 (2014). Matthew Jemielita*, Michael J. Taormina*, April DeLaurier, Charles B. Kimmel, and Raghuveer Parthasarathy (*Equal authors), "Comparing phototoxicity during the development of a zebrafish craniofacial bone using confocal and light sheet fluorescence microscopy techniques," J. Biophotonics 6: 920-928 (2013). Andrew F. Loftus, Vivian Hsieh, and Raghuveer Parthasarathy, "Modulation of membrane rigidity by the human vesicle trafficking proteins Sar1A and Sar1B," Biophys. Biochem. Res. Comm. 426: 585-589 (2012). Link. Raghuveer Parthasarathy, "Rapid, accurate particle tracking by calculation of radial symmetry centers," Nature Methods 9: 724-726 (2012), DOI: 10.1038/nmeth.2071 . Abstract: I introduce an algorithm for sub-pixel localization of imaged objects based on an analytic, non-iterative calculation of the best-fit radial symmetry center. This approach yields tracking accuracies that are near theoretical limits, similar to Gaussian fitting, but with orders of magnitude faster execution time, lower sensitivity to nearby particles, and applicability to any radially symmetric intensity distribution. I demonstrate the method with several types of data, including super-resolution microscopy images. Also: There's an associated News and Views article (by Alex Small). Michael J. Taormina, Matthew Jemielita, W. Zac Stephens, Adam R. Burns, Joshua V. Troll, Raghuveer Parthasarathy, and Karen Guillemin, "Investigating Bacterial-Animal Symbioses with Light Sheet Microscopy," Biol. Bulletin 223: 7-20 (2012). (Part of a special issue on "Discoveries in Animal Symbiosis in the 'omics' Age," August 2012.) Emily Goers Sweeney, J. Nathan Henderson, John Goers, Christopher Wreden, Kevin G. Hicks, Jeneva K. Foster, Raghuveer Parthasarathy, S. James Remington, and Karen Guillemin, "Structure and Proposed Mechanism for the pH-Sensing Helicobacter pylori Chemoreceptor TlpB," Structure 2012 (online publication date: June 14, 2012). DOI: http://dx.doi.org/10.1016/j.str.2012.04.021 . Cover art: My artwork for this paper is on the cover of the July issue of Structure. -- RP Raghuveer Parthasarathy, "Cars and Kinetic Energy - Some Simple Physics with Real-World Relevance," The Physics Teacher 50: 395-397 (2012). [Book Chapter] Raghuveer Parthasarathy, "Optics: Basic Physics," in Handbook of Biomedical Optics, ed. D.A. Boas, C. Pitris, N. Ramanujam (CRC Press, 2011). [Retracted] Christopher W. Harland, Miranda J. Bradley, and Raghuveer Parthasarathy, "Phospholipid bilayers are viscoelastic," Proc. Natl. Acad. Sci. USA 107: 19146-19150 (2010). Link The conclusions of this paper are wrong -- a consequence of insufficiently precise analysis. Our retraction notice was published in August 2011; you can read the page proof here or a version with graphs (not allowed in the official retraction notice) here. We deeply regret any confusion or wasted effort caused by our flawed paper, and we are happy to provide additional information or resources on technical issues related to the paper's methods. A postscipt: Spurred by these events, I've been thinking about whether there can exist a particle tracking method that is as accurate as direct fitting of Gaussian intensity profiles to particle images (the present state-of-the-art) but that is computationally much faster. I have come up with a new sort of tracking, based on maximizing radial symmetry, that works well. For a very brief description, see here. For a longer discussion and tests of the method, see my 2012 Nature Methods paper noted above, and my Particle Tracking page. -- RP. Edward I. Settles*, Andrew F. Loftus*, Alesia N. McKeown, and Raghuveer Parthasarathy, "The vesicle trafficking protein Sar1 lowers lipid membrane rigidity," Biophys. J. 99: 1539-1545 (2010). [Preview / Commentary paper; not a peer-reviewed article] Anica M. Wandler, Raghuveer Parthasarathy, and Karen Guillemin, "A greasy foothold for Helicobacter pylori," Cell Host & Microbe 7: 338-339 (2010). Yupeng Kong and Raghuveer Parthasarathy, "Different modulation mechanisms of attractive colloidal interactions by lipid and protein functionalization," Langmuir 26: 10541-10545 (2010). Kamil Godula, Marissa L. Umbel, David Rabuka, Zsofia Botyanszki, Carolyn R. Bertozzi, and Raghuveer Parthasarathy, "Control of the molecular orientation of membrane-anchored biomimetic glycopolymers," J. Am. Chem. Soc. 131: 10263-10268 (2009). Yupeng Kong and Raghuveer Parthasarathy, "Modulation of attractive colloidal interactions by lipid membrane functionalization," Soft Matter, 5: 2027-2029 (2009) Christopher W. Harland*, Zsofia Botyanszki*, David Rabuka, Carolyn R. Bertozzi, and Raghuveer Parthasarathy, "Synthetic trehalose glycolipids confer desiccation resistance to supported lipid monolayers," Langmuir 25: 5193-5198, (2009)