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1 Xiaofeng Wu,Kaeko Kamei,Hideki Sato,Shin-ichi Sato,Ryo Takano,Masatoshi Ichida,Hajime Mori,and Saburo Hara.High-level expression of human acidic and basic fibroblast growth factors in the silkworm,Bombyx mori L.using recombinant baculovirus.Protein Expression and Purification(USA).2001,21(1):192-200.
2 Kaeko Kamei,Xiaofeng Wu,Xinyan Xu,Kazuhiro Minami,Nguyen Tien Huy,Ryo Takano,Hisao Kato,and Saburo Hara The analysis of heparin-protein interactions using evanescent wave biosensor with regioselectively desulfated heparins as ligands.Analytical Biochemistry.2001,295:203-213
3 Wu Xiaofeng*,Zhaozheng Yin,Cuiping Cao,Ling Huang,Xingmeng Lu,Jianxin Liu,Weizheng Cui.Expression of human VEGF165 in silkworm(Bombyx mori L.)by using a recombinant baculovirus and its bioactivity assay.Journal of Biotechnology.2004,111(3):253-61.
4 Xiaofeng Wu*,Cao Cuiping,V.Shyam Kumar and Cui Weizheng.An innovative technique for inoculating recombinant baculovirus into the silkworm Bombyx mori using lipofectin,Research in Microbiology.2004,155(6):462-6.
5 Wu Xiaofeng*,Cao Cuiping,Xu Yaxiang,Lu Xingmen.Construction of a host range-expanded hybrid baculovirus of BmNPV and AcNPV,and knockout of Cysteinase gene f or more efficient expression.SciChinaC Life Sci.2004,47(5):406-415.
6 Yizhen Wang,Xiaofeng Wu*,Guangfu Liu,Cuiping Cao,Haiqing Huang,Zirong Xu,Jianxin Liu.Expression of porcine lactoferrin by using recombinant baculovirus in silkworm,Bombyx mori L.,and its purification and characterization.Appl Microbiol Biotechnol.2005,65:385-389
7 Huahua Du,Xiaofeng Wu*,Weifen Li,Wei Dai.Increased resistance to white spot syndrome virus in Procambarus clarkii by injection of envelope protein VP28 expressed using recombinant baculovirus.Aquaculture.2006,260,39-43.
8 Cuiping Cao,Xingmeng Lu,Na Zhao,Huipeng Yao,Xiaofeng Wu*,and Yeping Tan.Development of a rapid and efficient BmNPV baculovirus expression system for application in mulberry silkworm,Bombyx mori.Current Science.2006,91(12):1692-1697
9 Bing Wang,Xiaofeng Wu*,Na Zhao.Enhanced effect of fluorescent whitening agent on peroral infection for recombinant baculovirus in the host Bombyx mori.Current Microbiology.2007,54:5-8
10 Zhao Na,Yao Huipeng,Lan Lipan,Cao Cuiping,M.L.Umashankar,Lu Xingmeng,Wu Xiaofeng*,Wang Bing,Cui Weizheng,J.L.Cenis.Efficient production of canine interferon-alpha in silkworm Bombyx mori by use of a BmNPV/Bac-to-Bac expression system.Appl Microbiol Biotechnol.2008,78:221-226
11 Yao H,He F,Guo A,Cao C,Lu X,Wu X.Gene analysis of an antiviral protein SP-2 from Chinese wild silkworm, Bombyx mandarina Moore and its bioactivity assay.SciChinaC Life Sci.2008,51(10):879-84.
12Yao HP,Xiang XW,Chen L,Guo AQ,He FQ,Lan LP,Lu XM,Wu XF.Identification of the proteome of the midgut of silkworm,Bombyx mori L.by multidimensional liquid chromatography LTQ-Orbitrap mass spectrometry.Biosci Rep.2009,29(6):363–373
13 Hui-peng Yao,Lin Chen,Xingwei Xiang,Ai-qin Guo,Xing-meng Lu,Xiao-feng Wu.Proteomics identification and annotation of proteins of cell line of Bombyx mori,BmN cell.Biosci Rep.2010,30(3):209-15.
14Zhou L,Wu X,Lan L,Liu J.Expression of Trichoderma reesei endo-beta-glucanase II in silkworm,Bombyx mori L.by using BmNPV/Bac-to-Bac expression system and its bioactivity assay.Biotechnol Lett.2010,32:67–72
15 Xingwei Xiang,Rui Yang,Shaofang Yu,Cuiping Cao,Aiqin Guo,Lin Chen,Xiaofeng Wu,Weizheng Cui,Cenis J L.Construction of a BmNPV polyhedrin-plus Bac-to-Bac baculovirus expression system for application in silkworm,Bombyx mori.Applied Microbiology and Biotechnology.2010,87(1):289-95.
16 Soledad Chazarra,Salvador Aznar-Cervantes,Luis Sánchez-del-Campo,Juan Cabezas Herrera,Wu Xiaofeng,JoséLuis Cenis,JoséNeptuno Rodríguez-López.Purification and Kinetic Properties of Human Recombinant Dihydrofolate Reductase Produced in Chrysalides of Bombyx mori.Biotech Bioeng.2010,162(7):1834-46
17 Hao YIN,Xin-qin SHI,Bo SUN,Jing-jing YE,Zu-an DUAN1,Xiao-ling ZHOU,Wei-zheng CUI,Xiao-feng WU.Accumulation of 1-deoxynojirimycin in silkworm,Bombyx mori L.J Zhejiang Univ-Sci B(Biomed&Biotechnol).2010,11(4):286-291
18 Xingwei Xiang,Lin Chen,Aiqin Guo,Shaofang Yu,Rui Yang,Xiaofeng Wu.The Bombyx mori nucleopolyhedrovirus(BmNPV)ODV-E56 envelope protein is also a per os infectivity factor.Virus Research.2011,(155):69–75
19 Xingwei Xiang,Lin Chen,Xiaolong Hu,Shaofang Yu,Rui Yang,Xiaofeng Wu.Autographa californica multiple nucleopolyhedrovirus odv-e66 is an essential gene required for oral infectivity.Virus Research.2011,158(1-2):72-78.
20 Xingwei Xiang,Lin Chen,Shaofang Yu,Rui Yang,Cuiping Cao,Xiaofeng Wu.Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus(BmNPV)J Zhejiang Univ-Sci B(Biomed&Biotechnol).2012,12(2):111-117.
21 Xiaolong Hu,Lin Chen,Xingwei Xiang,Rui Yang,Shaofang Yu,Xiaofeng Wu.Proteomic analysis of peritrophic membrane(PM)from the midgut of fifth-instar larvae,Bombyx mori.Mol Biol Rep.2012,39(4):3427-34.
22 Lin Chen,Xiaolong Hu,Xingwei Xiang,Shaofang Yu,Rui Yang,and Xiaofeng Wu.Autographa californica multiple nucleopolyhedrovirus odv-e25(Ac94)is required for budded virus infectivity and occlusion-derived virus formation.Arch Virol.2012,157(4):617-25
23 Hu X,Chen L,Yang R,Xiang X,Wu X.Molecular characterization of a peritrophic membrane protein from the silkworm.Mol Biol Rep.2013,40(2):1087-95
24 Lin Chen,Rui Yang,Xiaolong Hu,Xingwei Xiang,Shaofang Yu,Xiaofeng WU.The formation of occlusion-derived virus was affected by the expression level of ODV-E25.Virus Research.2013,173(2):404-14
25 Lin Chen,Rui Yang,Xiao-Long Hu,Xing-Wei Xiang and Xiao-Feng Wu.Expression analysis of chlorophyllidαbinding protein,a secretory,red fluorescence protein in the midgut of silkworm,Bombyx mori.Insect Science.2013,21(1):20-30
26Xiang X,Shen Y,Yang R,Chen L,Hu X,Wu X.Bombyx mori nucleopolyhedrovirus BmP95 plays an essential role in BV production and nucleocapsid assembly.J Gen Virol.2013,(7)1669-1679
27.Lin Chen,Xingwei Xiang,Rui Yang,Xiaolong Hu,Xiaofeng Wu*Immobilization of Foreign Protein in BmNPV Polyhedra by fusion expression with partial polyhedrin fragments.2013,J Virol Methods.194(1-2):185-189
28.Xiaolong Hu,Rui Yang,Xiaofeng Wu.Molecular cloning and functional characterization of the dual oxidase(BmDuox)gene from the silkworm.2013,Plos One 8(8):e70118.doi:10.1371/journal.pone.0070118
29.Guobao Wang.Qin Zheng,Yunwang Shen,Xiaofeng Wu.Shotgun proteomic analysis of Bombyx mori brain:emphasis on regulation of behavior and development of the nervous system.Insect Sci.2014,21(1):20-30
30.Guobao Wang;Jianjia Zhang;Yunwang Shen;Qin Zheng;Min Feng;Xingwei Xiang,Xiaofeng Wu.Transcriptome analysis of the brain of the silkworm Bombyx mori infected with Bombyx mori nucleopolyhedrovirus:A new insight into the molecular mechanism of enhanced locomotor activity induced by viral infection.Journal of Invertebrate Pathology.2015,128:37-73
31.Zheng Q,Wu X,Zheng H,Zhou Y.Development of an enzyme-linked-immunosorbent-assay technique for accurate identification of poorly preserved silks unearthed in ancient tombs.Anal Bioanal Chem.Analytical and Bioanalytical Chemistry.2015,407(13):3861-3867
32.Chen L,Shen Y,Yang R,Wu X.Bombyx mori nucleopolyhedrovirus(BmNPV)Bm64 is required for BV production and per os infection.Virol J.2015,12(1):173-176.
33.Xiaolong Hu,Yunwang Shen,Qin zheng,Guobao Wang,Xiaofeng Wu,Chengliang Gong.Bm59 is an early gene,but is unessential for the propagation and assembly of Bombyx mori nucleopolyhedrovirus.Molecular Genetics and Genomics,2016,291(1):145-54
34.Guobao Wang.Qin Zheng,Yunwang Shen,Xiaofeng Wu.Shotgun proteomic analysis of Bombyx mori brain:emphasis on regulation of behavior and development of the nervous system.Insect Sci.2016,23:15-27
35.Yang R,Zhang J,Feng M,Wu X.Identification of Bombyx mori nucleopolyhedrovirus bm58a as an auxiliary gene and its requirement for cell lysis and larval liquefaction.J Gen Virol.2016,97(11):3039-3050
36.Qin Zheng,Yunwang Shen,Xiangshuo Kong,Jianjia Zhang,Min Feng,Xiaofeng Wu*.Protein-Protein Interactions of the Baculovirus Per Os Infectivity Factors in the PIF Complex.J Gen Virol.,2017,98(4):853-861
37.Yang Rui,Zhang Jianjia,Feng Min,Xiaofeng Wu*.Identification of Bombyx mori nucleopolyhedrovirus bm58a as an auxiliary gene and its requirement for cell lysis and larval liquefaction.Journal of General Virology,2016,97(11):3039-3050
38.Xiaolong Hu,Yunwang Shen,Qin Zheng,Guobao Wang,Xiaofeng Wu*.Bm59 is an early gene,but is unessential for the propagation and assembly of Bombyx mori nucleopolyhedrovirus.Molecular Genetics and Genomics,2016,291(1):145-54
39.Guobao Wang.Qin Zheng,Yunwang Shen,Xiaofeng Wu*.Shotgun proteomic analysis of Bombyx mori brain:emphasis on regulation of behavior and development of the nervous system.Insect Sciences,2016,23(1):15-27
40.Qin Zheng,Yunwang Shen,Xiangshuo Kong,Jianjia Zhang,Min Feng,Xiaofeng Wu*.Protein-Protein Interactions of the Baculovirus Per Os Infectivity Factors in the PIF Complex.Journal of General Virology,2017,98(4):853-861
41.Min Feng,Xiangshuo Kong,Jianjia Zhang,Weifan Xu,Xiaofeng Wu*.Identification of a novel host protein SINAL10 interacting with GP64 and its role in BmNPV infection.Virus Research,2018,247:102-110
42.Jianjia Zhang,Min Feng,Ying Fan,Weifan Xu,Qin Zheng,Xiaofeng Wu*.Networks of protein-protein interactions among structural proteins of budded virus of Bombyx mori nucleopolyhedrovirus.Virology,2018,518:163-171
43.Yunwang Shen,Haiping Wang,Weifan Xu,Xiaofeng Wu*.Bombyx mori nucleopolyhedrovirus orf133 and orf134 are involved in the embedding of occlusion-derived viruses into polyhedra.Journal of General Virology,2018,99(5):717-729
44.Min Feng,Jianjia Zhang,Weifan Xu,Haiping Wang,Xiaofeng Wu*.BmNPV utilizes a clathrin and dynamin dependent endocytosis entry pathway into BmN cells.Virus Research,2018,253:12-19
45.Yunwang Shen,Min Feng,Jianjia Zhang,Xiaofeng Wu*.Bombyx mori nucleopolyhedrovirus ORF40 is essential for budded virus production and occlusion-derived virus envelopment.Journal of General Virology,2018,Apr 19.doi:10.1099/jgv.0.001066.[Epub ahead of print]
国内一级学报
1.曹翠平,吴小锋*.2004,重组昆虫杆状病毒构建和筛选技术进展.昆虫学报,47(6):837-843 ISSN 0454-6296
2.吴小锋*,曹翠平,崔为正。2005,应用脂质体介导技术改变重组杆状病毒感染方法的。昆虫学报,48(1):143-146 ISSN 0454-6296
3.曹翠平,吴小锋*.2005,利用重组杆状病毒在家蚕中表达人血管内皮细胞生长因子
的研究。中国生物化学与分子生物学报,21(2):234-240 ISSN 1007-7626
4.曹翠平,吴小锋*,鲁兴萌。2005昆虫细胞无血清培养研究。细胞生物学杂志。
Vol.27(2):127-132 ISSN0253-9977(现为二级)
5.Xiaofeng Wu(吴小锋)*,Xu Yaxiang,Shen Guoxin,Kaeko Kamei,Ryo Takano and Saburo
Hara.Surface plasmon resonance analysis to evaluate the importance of heparin sulfate
groups'binding with human aFGF and bFGF.2003 J Zhejiang Univ Sci.4:86-94.(浙大学报
英文版)
6.吴小锋*,曹翠平,许雅香,鲁兴萌.2004,BmNPV和AcNPV扩大寄主域杂交重组病毒
表达载体的构建和改进.中国科学C辑(生命科学),34(2),156-164.
7.曹翠平,吴小锋*。2006,昆虫杆状病毒应用于哺乳动物基因治疗的研究进展。微生物学报。46(4):668-672
8.吴小锋*,曹翠平,鲁兴萌。2005,昆虫杆状病毒表达系统中重组蛋白质的降解及其对策,蚕业科学,(4):382-388
9.姚慧鹏,吴小锋*,倪燕军,徐慧。2005,家蚕丝蛋白基因密码子偏爱性分析,蚕业科学,31(增刊):39-43
10.许雅香,吴小锋,朱玉芳,许梓荣,沈卫德。2005,利用宿主范围扩大的杂交病毒HyNPV在家蚕中表达对虾白斑综合症病毒VP19基因。生物工程学报,21(5):837-839
11.吴小锋*,曹翠平,鲁兴萌。2006,利用细菌转座子构建适用于家蚕的Bac-to-Bac快速基因表达系统。蚕业科学,32(2):183-188 ISSN:0257-4799
12.吴小锋*。2006,家蚕核型多角体病毒(BmNPV)的基因组结构及其表达模式。病毒学报。22(4):324-328.
13.姚慧鹏,郭爱芹,何芳青,鲁兴萌,吴小锋。2008,家蚕幼虫中肠蛋白质组学。生物工程学报,24(1):89-94
14.何芳青,吴小锋。2008,昆虫杆状病毒表达系统的新技术。蚕桑通报,39(2):10-13
15.曹翠平,兰丽盼,姚慧鹏,何芳青,郭爱芹,吴小锋。家蚕Polh+Bac-to-Bac杆状病毒表达系统的构建。2008,蚕业科学,34(2):237-243.
16.姚慧鹏,吴小锋。昆虫类免疫球蛋白结构和功能研究进展。2008,昆虫学报,51(5):530-536
17.姚慧鹏,何芳青,郭爱芹,曹翠平,鲁兴萌,吴小锋。中国野蚕一种强抗病毒蛋白的基因分析和活性鉴定。2008,中国科学C辑:生命科学,38(6):521-526
18.兰丽盼,吴小锋。昆虫杆状病毒研究新进展。2008,农业生物技术学报。16(6):1056-1060。
姚慧鹏,曹翠平,吴小锋。中国野蚕抗病毒蛋白Lipase的基因克隆和活性分析。2008,蚕业科学,34(3):466-471
19.曹翠平,郭爱芹,相兴伟,陈琳,何芳青,姚慧鹏,吴小锋.利用BmNPV多角体包埋外源蛋白的观察.蚕业科学,2009,35(4):790-795
20.何芳青,陈琳,姚慧鹏,郭爱芹,相兴伟,吴小锋.家蚕类免疫球蛋白(Hemolin)基因的克隆及表达特征和抗菌活性研究。2010,蚕业科学,36(1):40-45
21.曹翠平,于少芳,郭爱芹,杨锐,相兴伟,陈琳,吴小锋.家蚕BmNPV多角体蛋白片段引导外源蛋白质固定入多角体的研究.2010,蚕业科学,36(2):275-282
22.相兴伟,陈琳,于少芳,杨锐,吴小锋.BmNPV ODV囊膜蛋白基因的融合表达及引导外源蛋白质进入多角体的研究。2010,蚕业科学,36(5):785-791
23.相兴伟,吴小锋。昆虫包涵体衍生病毒囊膜蛋白的分子生物学。2010,昆虫学报,53(7):809-817。
24.于少芳,吴小锋。昆虫杆状病毒表达系统提高重组蛋白表达量的新技术,2010,蚕桑通报,41(3):6-9
25.于少芳,陈琳,相兴伟,胡小龙,杨锐,吴小锋.利用家蚕BmN细胞表达重组蝎毒素蛋白及其杀虫效果分析.2011,蚕业科学,37(3):449-455。
26.相兴伟,杨锐,陈琳,胡小龙,于少芳,吴小锋。家蚕BmNPV多角体蛋白与外源蛋白共包埋入多角体的研究。2011,病毒学报,27(4)366-371
27.于少芳,陈琳,吴小锋.质型多角体病毒与杆状病毒多角体同源性的比较。2011,蚕业科学,37(5):883-891。
28.胡小龙,吴小锋。昆虫中肠围食膜构造与功能的研究进展。2012,蚕业科学,38(6):1110-1117
29.王国宝,吴小锋。昆虫杆状病毒诱导宿主出现行为变化及其分子机制。2013,蚕业科学,39(5):1005-1010
30.郑秦,吴小锋,郑海玲,周旸。利用酶联免疫法鉴定古代丝织品。蚕业科学,2014,40(3):39-45
31.陈琳,吴小锋。昆虫杆状病毒病毒粒子蛋白质组成及其功能。2014,蚕业科学,40(3):81-91
32.张健家,沈运旺,吴小锋。昆虫杆状病毒的经口感染因子。2014,蚕业科学,40(4):730-736
33.冯敏,吴小锋。杆状病毒囊膜蛋白与宿主细胞表面受体的相互作用。2014,蚕业科学,40(5):911-916
34.郑秦,吴小锋。昆虫杆状病毒经口感染因子P74的结构与功能。2016,病毒学报,32(4):523-527
35.许伟凡,吴小锋。昆虫杆状病毒囊膜F蛋白研究进展,蚕业科学,2017,第2期
36.王海萍,吴小锋。昆虫杆状病毒egt基因研究进展。蚕桑通报,48(1):10-13
37.杨锐,冯敏,吴小锋。苜蓿银纹夜蛾核型多角体病毒囊膜蛋白GP64与寄主细胞互作蛋白的初步鉴定。2017,43(5):0789-0795
38.郑秦,孔祥硕,王海萍,许伟凡,吴小锋。家蚕核型多角体病毒经口感染因子P74与家蚕互作蛋白的初步筛选。蚕业科学,2018,44(1)56-63
39.郑秦,范颖,王海萍,许伟凡,孔祥硕,吴小锋。杆状病毒经口感染因子之间相互作用分析。病毒学报,2018,34:58-93