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李卫芬,浙江大学农学博士,研究员,博士生导师。浙江省151第二层次人才。 主要从事微生物基因工程、益生菌在畜禽、水产上的作用效果及机理以及有益菌基因表达系统构建等方面的研究和教学工作。先后主持国家自然科学基金、973课题、国家支撑项目子课题、浙江省科技厅等项目20多项。获国家科技进步二等奖1项,浙江省科技科进步二等奖4项,国家发明专利8项。在国内外学术刊物上发表论文100余篇,其中SCI 60多篇。经多年研究,阐明了酶制剂、益生菌、植物提取物和免疫肽在畜禽或水产养殖中作用机理,为益生菌等生物饲料添加剂生产及其应用和推广提供了理论基础和技术支撑。 近年来,先后与哈佛大学黏膜免疫和生物学研究中心,宾夕法尼亚州立大学,俄力冈州立大学,法国农业科学研究院,美国SABINSA集团公司等开展学术合作与交流。 教学与课程 讲授课程: 1.水化学(本科生课程) 2.分子生物学实验技术(研究生课程) 3.生物饲料(研究生课程) 科研项目 主持/主参“973”课题、“863”课题、国家自然科学基金、国家支撑项目子课题、浙江省科技厅等20余项。 1.益生芽孢杆菌对断奶仔猪自噬的影响及其缓解氧化应激的分子机理(国家基金) 2.防治鸡坏死性肠炎专用微生物态制剂的研制与应用(浙江省公益技术研究农业项目) 3.幼龄动物等畜禽多用微生态制剂创制与中试示范(十二五支撑项目子课题) 4.解淀粉芽孢杆菌对巨噬细胞极化的影响及其信号通路解析(国家基金) 5.免疫调节肽PMP研制与中试(十二五支撑项目子课题) 6.饲用生物制剂提高动物免疫及抗氧化功能的作用机制研究(“863”子课题) 7.鼠李糖乳杆菌对仔猪肠道免疫功能的影响及其TLRs信号传导途径的研究(教育部博士点基金) 8.连接肽影响NSP杂合酶结构与特性的机理研究(国家基金) 9.防治鸡球虫病口服生物制剂的研制及产业化(省重点项目) 10.有益微生物对养殖水环境的调控作用及机理(“973”子课题) 11.畜禽用功能型微生态制剂的研制及产业化(省重大专项) 12.动物加工产品绿色防腐技术的研究及产业化应用(省重大专项) 13.热纤维梭菌β-葡聚糖酶耐热的分子机理研究(国家基金) 14.生物防腐剂Nisin-AP杂合基因构建及表达的关键技术研究(省重点项目) 15.高效分泌型芽孢杆菌表达系统构建的关键技术研究(省科技厅) 16.高效、长效、无污染生态型底质改良剂产业化研究(宁波市科技局) 17.β-葡聚糖酶N-端结构与其热稳定性关系的研究(国家基金) 18.新型绿色生物饲料添加剂MSP-1中试及产业化(宁波市科技局) 19.耐热β-葡聚糖酶重组基因的构建及表达(国家基金) 研究与成果 益生菌是指通过改善肠道菌群平衡而对宿主产生有益作用的活微生物。益生菌作为绿色饲料添加剂目前已被广泛应用于畜牧养殖业,其促生长机理与改善动物肠道屏障及提高机体免疫和抗氧化功能密切相关。本研究团队在成功筛选获得一株具免疫调节和抗氧化功能益生菌BaSC06基础上,采用western blot、免疫荧光和透射电镜等方法系统研究了其发挥免疫调节和抗氧化功能的作用机理,取得如下研究进展: 一、通过构建巨噬细胞极化和肠上皮细胞氧化应激模型,成功筛选一株同时具有免疫调节和抗氧化功能的益生菌BaSC06; 二、饲喂BaSC06可提高小鼠体内巨噬细胞数量和含量,直接促进巨噬细胞M1型极化,增加其对ST的杀伤功能;同时BaSC06调节的肠道菌群能够促进巨噬细胞向M2型极化,维持肠道的微生态稳态和炎症平衡,二种巨噬细胞共同发挥作用抵抗ST感染。 三、BaSC06可通过调控Nrf2-Keap1信号通路提高细胞和机体抗氧化酶的表达,同时通过下调NADPH氧化酶的表达,降低ROS产生和线粒体损伤,从而提高动物抗氧化功能。 四、BaSC06还可通过p38MAPK信号通路调节自噬来提高细胞和机体先天性免疫反应和抗氧化功能,缓解病原菌感染和氧化应激所造成的损伤。 专利成果 1.里氏木霉菌株及其用途(ZL 02111421.8)(排名第3) 2.凝结芽孢杆菌菌株及其用途(ZL200610049823.9)(排名第1) 3.一种冬虫夏草发酵菌粉的制备方法(ZL200610050272.8)(排名第1) 4.乳酸乳球菌食品级载体(ZL.200610154649.4)(排名第1) 5.一种冷却肉复合防腐保鲜剂(ZL.200810162614.4)(排名第2) 6.一种防治对虾白斑病的口服生物制剂(ZL.201010130589.9)(排名第1) 7.一种防治鸡球虫病的活载体疫苗制备方法及其用途和由其制成的口服生物制剂(ZL.201010220272.4)(排名第1) 8.猪用复合干扰素诱生剂(201110306648)(排名第1) 9.假单胞菌及其用途(ZL.201210398352.8)(排名第1) 10.表达大肠杆菌毒素蛋白mazF基因的表达盒mazF-cassette及其构建法和用途(ZL.201110223046.6)(排名第1) 研究生培养 本课题组主要研究方向为生物饲料与健康养殖,其中益生菌改善动物肠道和机体健康方面的机理研究处于国内领先水平。 自2003年以来,共培养研究生57位。博士研究生在国家/学校留学基金委的资助下,公派留学至哈佛大学、宾夕法尼亚州立大学、法国农科院等著名院校开展合作研究。所有学生均参加过两次以上学术会议,绝大多数博士研究生曾出国参加学术交流。此外,95%以上的学生获得过各类奖学金及省校级荣誉称号,包括国家奖学金(营养学科每年评选3名左右)、校院级奖学金、诺伟司国际研究生奖学金优胜奖(学院每年评选1名赴诺伟司美国总部实习)、省级优秀毕业生(学院每年评选博士1-2名,硕士4名左右)、校级优秀毕业生等。 课题组学术氛围浓厚,师生关系融洽,就业前景良好。已毕业25位博士生基本就职于大学和科研院所,已毕业32位硕士生在国内外著名企业工作。毕业学生工作业绩突出,成为所在单位的科技精英、业务骨干。 相信怀有理想的你加入到我们这个充满学术氛围与活力的研究团队,将激发出你的科研热情,发挥出你的聪明才智,享受着科学实验,并不断取得令人欣喜的科研成果,成为德才兼备的一流人才。

研究领域

1.饲用益生菌的研究与开发 2.生物饲料与健康养殖 3.微生物基因工程

近期论文

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1.W Du,H Xu,X Mei,X Cao,L Gong,Y Wu,Y Li,D Yu,S Liu,Y Wang,W Li*.Probiotic Bacillus enhance the intestinal epithelial cell barrier and immune function of piglet.Beneficial Microbes,2018 2.Yuanyuan Wang,Yibing Wang,Han Xu,Xiaoqiang Mei,Li Gong,Baikui Wang,Weifen Li†,Shouqun Jiang.Direct-fed Glucose Oxidase and its combination with B.amyloliquefaciens SC06 on growth performance,meat quality,intestinal barrier,antioxidative status and immunity of yellow-feathered broilers.Poultry Science,2018 3.Bai-kui WANG,Yu-long MAO,Gong LI,Xin XU,Shou-qun JIANG,Yi-bing WANG,Wei-fen LI.Glycyrrhizic acid activates chicken macrophages and enhances their salmonella-killing capacity in vitro[J].Journal of Zhejiang University-SCIENCE B,2018. 4.Wang Y,Wu Y,Wang Y...&Li W.Antioxidant Properties of Probiotic Bacteria.Nutrients,2017 5.Wang Y,Wu Y,Wang B...&Li W.Effects of probiotic Bacillusas a substitute for antibiotics on antioxidant capacity and intestinal autophagy of piglets.AMB Express,2017:52 6.Wang Y,Du W,Lei K...&Li W.Effect of Dietary Bacillus lichenuformison Gut Physical Barrier,Immunity and Reproductive Hormones of Laying Hens.Probiotics Antimicrob Proteins,2017:1-8 7.Wu Y,Wang Y,Zhou H,...&Li W.ProbioticBacillus amyloliquefaciensSC06 induces Autophagy to Protect against Pathogens in Macrophages.Front Microbiol,2017 8.Fu A,Wang Y,Wu Y,...&Li W.Echinacea purpurea Extract Polarizes M1 Marcrophages through the Activation of JNK.J Cell Biochem,2017 9.YL Li,YY Wang,YP Wu,BK Wang,X Chen,X Xu,HL Chen,WF Li,XG Xu,2017.Echinacea pupurea extracts promote murine dendritic cell maturation by activation of JNK,p38 MAPK and NF-κB pathways.Developmental and Comparative Immunology,73:21-26. 10.LQ Fu#,YL Li#,SL Hu,AK Fu,YP Wu,YY Wang,XM Tong and WF Li,2017.Pidotimod exacerbates allergic pulmonary infection in an OVA mouse model of asthma.Molecular Medicine Reports.doi:10.3892/mmr.16(4):4151-4158. 11.Fu LQ,Zhang XP,Wang YB,et al(2017).Nitrogen removal characteristics of Pseudomonas stutzeri,F11 and its application in grass carp culture.Fisheries Science 83(1):89-98. 12.Gong,L.,Huang,Q.,Fu,A.,Wu,Y.,Li,Y.,Xu,X.,...&Li,W.(2017).Spores of two probiotic Bacillus species enhance cellular immunity in BALB/C mice.Canadian journal of microbiology,(000),1-8. 13.Wang Y,Wu Y,Wang Y,...&Li W.Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1 signaling pathway and decreasing ROS production.Appl Microbiol Biotechnol,2016:1-12 14.YL Li,AK Fu,HL Chen,WF Li and LQ Fu,2016.Potentiating effect of pidotimod on immune responses of chickens to Newcastle disease vaccines.Italian journal of animal science,15:536-544. 15.Wang Y B,Du W,Fu A K,et al.Intestinal microbiota and oral administration of Enterococcus faecium associated with the growth performance of new-born piglets.[J].Beneficial Microbes,2016:1-10 16.Yulong Mao,Baikui Wang,Xin Xu,Wei Du,Weifen Li*,andYouming Wang.Glycyrrhizic Acid Promotes M1 Macrophage Polarization in Murine Bone Marrow-Derived Macrophages Associated with the Activation of JNK and NF-κB[J].Mediators of Inflammation,2015,(2):1-12 17.Zhiwei Lin,Yanyun Shi,Bin Deng,Xiangfei Mao,Dongyou Yu*,Weifen Li*.Protective immunity against Eimeria tenella infection in chickens following oral immunization with Bacillus subtilis expressing Eimeria tenella 3-1E protein.Parasitology Research.2015,114(9):3229-3236. 18.Lei K,Li YL,Wang Y,Wen J,Wu HZ,Yu DYand Li WF*.Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice.J Zhejiang Univ Sci B.2015,16(6):487-95.doi:10.1631/jzus.B1400342 19.Quan Liang,Xiaoping Zhang,Khui Hung Lee,Yibing Wang,Kan Yu,Wenying Shen,Luoqin Fu,Miaoan Shu,Weifen Li*.Nitrogen removal and water microbiota in grass carp culture following supplementation with Bacillus licheniformis BSK-4.World J Microbiol Biotechnol.2015,31(11):1711-1718. 20.Deng D,Fu LQ,Zhang XP*,Zheng JJ,Peng LS,Sun JD,Zhu HY,Wang YB,Li WF*,Wu XX,Wu D.The denitrification characteristics of Pseudomonas stutzeri SC221-M and its application to water quality control in grass carp aquaculture.Plos one,2014,9(12):e114886. 21.Shenglan Hu,Xudong Fu,Aikun Fu,Wei Du,Jian Ji,Weifen Li*.The regulatory peptide pidotimod facilitates M2 macrophage polarization and its function.Amino Acid.2014.46(5):1177-1185. 22.Zhang XP,Shu MA,Wang YB,Fu LQ,Li WF,Deng B,Liang Q,Shen WY.Effects of photosynthetic bacteria on water quality,microbial community structure and diversity in Grass Carp culture.World J Microbiol Biotechnol.2014,30(9),2523-2531. 23.Zhang XP,Peng LS,Wang YB,Liang Q,Deng B,Li WF,Fu LQ,Yu DY,Shen WY,Wang ZP.Effect of dietary supplementation of probiotic on performance and intestinal microflora of Chinese soft-shelled turtle(Trionyx Sinensis).Aquaculture Nutrition.2014,20(6),667–674. 24.Rajput,I.R.,Hussain,A.,Li,Y.L.,Zhang,X.,Xu,X.,Mao,Y.L.,Yu,D.Y.,&Li,W.F*..Saccharomyces boulardii and Bacillus subtilis B10 modulate TLRs mediated signaling to induce immunity by chicken bone marrow dendritic cells.Journal of cellular biochemistry.2014.115:189-198 25.YL Li,K Lei,X Xu,IR Rajput,DY Yu and WF Li,Protective effects of Bacillus subtilis B10 against hydrogen peroxide-induced oxidative stress in murine macrophage cell line.International Journal of Agriculture and Biology.2013,15:927-932. 26.K Lei,YL Li,DY Yu,IR Rajput and WF Li.Influence of dietary inclusion of Bacillus licheniformis on laying performance,egg quality,antioxidant enzymes activities and intestinal barrier function of laying hens.Poultry Science.2013,92(9):2389-95 27.Jian Ji,Shenglan Hu,Zhiwen Cui,Weifen Li*.Probiotic Bacillus amyloliquefaciens mediate M1 macrophage polarization in mouse bone marrow-derived macrophages.Archives of Microbiology.2013.DOI:10.1007/s00203-013-0877-7. 28.Jian Ji,Shenglan Hu,Mingzhu Zheng,Wei Du,Qinqin Shang,Weifen Li*.Bacillus amyloliquefaciens SC06 inhibits ETEC-induced proinflammatory responses by suppression of MAPK signaling pathways in IPEC-1 cells and diarrhea in weaned piglets.Livestock Science.2013.DOI:http://dx.doi.org/10.1016/j.livsci.2013.09.017. 29.Jian Ji,Shenglan Hu,Weifen Li*.Probiotic Bacillus amyloliquefaciens SC06 prevents bacterial translocation in weaned mice.Indian J Microbiol.2013.53(3):323–328. 30.Imran Rashid Rajput,Li Ya Li,Xu Xin,Yi Huang,Zhi Wen Cui,Dong You Yu and Weifen Li*.Supplementation of Saccharomyces boulardii and Bacillus subtilis B10 to increase digestive enzymes activity,antioxidation functioning and blood homeostasis in broiler.International Journal of Agriculture and biology.International Journal of Agriculture and Biology.2013.15:231-237. 31.Imran Rashid Rajput,Yali Li,Xin Xu,Lijuan Zhai,Bing bing Wu,Dongyou Yu and Weifen Li*.Effect of Saccharomyces boulardii and Bacillus subtilis B10 on intestinal ultrastructure modulation and mucosal immunity development in broiler.Poultry Science.2013.92(4):956-965. 32.XP Zhang,LQ Fu,B Deng,Q Liang,JJ Zheng,JD Sun,HY Zhu,LS Peng,YB Wang,WY Shen,WF Li*.Bacillus subtilis SC02 supplementation causes alterations of the microbial diversity in grass carp water.World Journal of Microbiology and Biotechnology.2013.29(9):1645-1653. 33.QinHuang,Xin Xu,Yu-long Mao,Yi Huang,Imran R.Rajput,Wei-fenLi*.Effects of Bacillus subtilis B10 on viability and biological functions of murine macrophages.Animal Science,2013,92(4):956-965. 34.Zhiwei Lin,Bin Deng,Zhihua Jiao,Bingbing Wu,Xin Xu,Dongyou Yu,Weifen Li*.A versatile mini-mazF-cassette for marker-free targeted genetic modification in Bacillus subtilis.Journal of Microbiological Methods.2013.95(2):207-214. 35.Yi Huang,Yali Li,Qin Huang,Zhiwen Cui,Dongyou Yu,Imran Rashid Rajput,Caihong Hu,Weifen Li*.Effect of orally administered Enterococcus faecium EF1 on intestinal cytokines and chemokines production of sucking piglets.Pakistan Veterinary Journal.2012,32(1):82-84. 36.Li Weifen,Zhang Xiaoping,Song Wenhui,Deng Bin,Liang Quan,Fu Luoqin,Zheng Jiajia,Wang yue,Yu Dongyou*.Effects of Bacillus preparations on immunity and antioxidant activities in Grass Carp(Ctenopharyngodon idellus).Fish Physiology and Biochemistry.2012,38(6):1585-1592. 37.Xin Xu,Qin Huang,Zhiwen Cui,Yulong Mao,Yali Li,Yi Huang,Imran Rashid Rajput,Dongyou Yu,Weifen Li*.Immunomodulatory effects of Bacillus subtilis(natto)B4 spores on murine macrophages.Microbiology and Immunology.2012,56(12):817-824. 38.Qin Huang,Xin Xu,Yu-long Mao,Yi Huang,Imran Rashid Rajput,Weifen Li*.Effects of Bacillus subtilis B10 on viability and biological functions of murine macrophage.Animal Science Journal.2012,12(20). 39.Wei fen Li,Imran Rashid Rajput,Xin Xu,Ya li Li,Jian Lei,Qin Huang,Min qi Wang.Effects of Probiotic(Bacillus Subtilis)on Laying Performance,Blood Properties and Microflora of Shaoxing Duck.International Journal of Poultry Science,2011,10(8):583-589. 40.Ling-Lin Fu,Yanbo Wang,Zheng-Cun Wu,Weifen Li*.In vivo assessment for oral delivery of Bacillus subtilis harboring a viral protein(VP28)against white spot syndrome virus in Litopenaeus vannamei.Aquaculture.2011,322-323:33-38. 41.Xuxia Zhou,Yanbo Wang,Jiangtao Yao,Weifen Li*.Inhibition ability of probiotic,Lactococcus lactis,against A.hydrophila and study of its immunostimulatory effect in tilapia(Oreochromis niloticus).International Journal of Engineering,Science and Technology.2010,2(7):73-80. 42.Xuxia Zhou,Yanbo Wang,Qing Gu,Weifen Li*.Effect of dietary probiotic,Bacillus coagulans,on growth performance,chemical composition,and meat quality of Guangxi Yellow chicken.Poultry Science.2010,89:588-593. 43.Ling-Lin Fu,Jiang-Bing Shuai,Zi-Rong Xu,Jian-Rong Li*,Wei-fen Li*.Immune responses of Fenneropenaeus chinensis against white spot syndrome virus after oral delivery of VP28 using Bacillus subtilis as vehicles.Fish&Shellfish Immunology.2010,28(1):49-55. 44.Ling-lin Fu,Wei-fen Li*.Protection of Fenneropenaeus chinensis(Osbeck,1765)against the white spot syndrome virus using specific chicken egg yolk immunoglobulins by oral delivery.Aquaculture research,2010,41(11):1806-1816. 45.Dong Niu,Xu-xia Zhou,Tao-yan Yuan,Zhi-wei Lin,Hui Ruan Wei-fen Li*.Effect of C-terminal Domains and deletion of N-or C-terminal residues of catalytic domain on thermostability and enzymatic activity of Lichenase from Clostridium thermocellum.Biotechnology Letters.2010,32(7):963-967. 46.Wen-Ying Shen,Ling-Lin Fu,Wei-Fen Li*,Yao-Rong Zhu.Effects of dietary supplementation with Bacillus subtilis on growth performance,immune response and antioxidant activities of the shrimp(Litopenaeus vannamei).Aquaculture Research.2010,41(11):1691-1698. 47.Xuxia Zhou,Yanbo Wang,Weifen Li*.Effects of different dietary selenium sources(selenium nanoparticle and selenomethionine)on growth performance,muscle composition and glutathione peroxidase enzyme activity of crucian carp(Carassius auratus gibelio).Aquaculture,2009,291:78-81. 48.Xu-Xia ZHOU,Yan-Bo WANG,Wei-fen Li*.Effect of probiotic on larvae shrimp(Penaeus vannamei)based on water quality,survival rate and digestive enzyme activities.Aquaculture,2009,287(3-4):349-353. 49.Xuxia Zhou,Ziqiang Tian,Yanbo Wang,Wei-fen Li*.Effect of treatment with probiotics as water additives on tilapia(Oreochromis niloticus)growth performance and immune response.Fish Physiology and Biochemistry.2009,2:1-9. 50.Chun-xia Hu,Zi-rong Xu,Wei-fen Li*,Niu Dong,Ping Lu,Ling-lin Fu.Secretory expression of K88(F4)fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice.Biotechnology Letters.2009,31(7):991-997. 51.Xu-xia Zhou,Yan-bo Wang,Yuan-jiang Pan,Wei-fen Li*.Investigation of the cytotoxicity of apidaecin on intestinal epithelial cells of tilapia(Oreochromis niloticus)in vitro.Aquaculture international.2008,16(6):591-600. 52.Xu-xia Zhou,Yuan-jiang Pan,Yan-bo Wang,Wei-fen Li*.Optimization of Medium Composition for Nisin Fermentation with Response.Surface Methodology.Journal of Food Science.2008,73(6):245-249. 53.Dong-you Yu,XL Li,Wei-fen Li*.Effect of montmorillonite superfine composition on growth performance and tissue lead level in pigs.Biological Trace Element Research.2008,125(3):229-235. 54.Ling-lin Fu,Zi-rong Xu,Jiang-bin Shuai,Chun-xia Hu,Wei Dai,Wei-fen Li*.High-level secretion of a chimeric thermostable lichenase from Bacillus subtilis by screening of site-mutated signal peptides with structural alterations.Current Microbiology,2008,56(3):287-292. 55.Ling-lin Fu,Wei-fen Li*,Hua-hua Du,Wei Dai,Zi-rong Xu.Oral vaccination with envelope protein VP28 against white spot syndrome virus in Procambarus clarkii using Bacillus subtilis as delivery vehicles.Letters in applied microbiology,2008,46(5):581-586. 56.Yan-bo Wang,Zi-qiang Tian,Jiang-tao Yao,Wei-fen Li*.Effect of probiotics,Enteroccus faecium,on tilapia(Oreochromis niloticus)growth performance and immune response.Aquaculture,2008,3-4:203-207. 57.Xu-xia Zhou,Yan-bo Wang,Yuan-jiang Pan,Wei-fen Li*.Nisin controlled extracellular production of apidaecin in Lactococcus lactis.Applied Microbiology and Biotechnology.2008,78:947-953. 58.Xu-xia Zhou,Yan-bo Wang,Yuan-jiang Pan*,Wei-fen Li*.Differences in amino acids composition and coupling patterns between mesophilic and thermophilic proteins.Amino Acids,2008,34(1):25-33. 59.Xu-xia Zhou,Yan-bo Wang,Wei-fen Li*.Effect of feeding apidaecin on common carp(Cyprinus carpio)growth performances and immune function.Aquaculture.2008,279(1-4):108-112. 60.Xu-xia Zhou,Yuan-jiang Pan,Yan-bo Wang,Wei-fen Li*.In vitro assessment of gastrointestinal viability of two photosynthetic bacteria,Rhodopseudomonas palustris and Rhodobacter sphaeroides.Journal of Zhejiang University(Science).2007,8(9):677-683. 61.Ling-lin Fu,Zi-rong Xu,Wei-fen Li*,Jiang Bing Shuai,Ping Lu,Chun Xia Hu.Protein secretion pathways in Bacillus subtilis:Implication for optimization of heterologous protein secretion.Biotechnology Advances,2007,25:1-12. 62.Yan-bo Wang,Jian-zhong Han,Wei-fen Li*,Zi-rong Xu.Effect of different selenium source on growth performances,glutathione peroxidase activities,muscle composition and selenium concentration of allogynogenetic crucian carp(Carassius auratus gibelio).Animal Feed Science and Technology.2007,134(3-4):243-251. 63.Qin Huang,Zi-Qiang Tian,Wei-fen Li*.Progress in the Development of Lactococcus lactis as a Mucosal Vaccine Delivery Vehicle.International Journal of Biomedical and Pharmaceutical Sciences,2007,1(2):124-134. 64.Ping Lu,Ming-Guang Feng,Wei-fen Li*,Chun-Xia Hu.Construction and characterization of a bifunctional fusion enzyme of Bacillus-sourcedβ-glucanase and xylanase expressed in Escherichia coli.FEMS Microbiology Letters.2006,261:224-230. 65.Xu-xia Zhou,Yuan-jiang Pan,Wei-fen Li*.The nisin-controlled gene expression system:Construction,application and improvements.Biotechnology Advances.2006,24:285-295. 66.Wei-fen Li*,Guo-xia Ma,Xu-xia Zhou.Apidaecin-type peptides Biodiversity,structure–function relationships and mode of action.Peptides.2006,9:2350-2359. 67.Wei-fen Li*,Xu-xia Zhou,Ping Lu.Structural features of thermozymes.Biotechnology Advances.2005,23(4):271-281. 68.Wei-fen Li*,Xu-xia Zhou,Ping Lu.Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis.Research in Microbiology.2004,155(8):605-610. 69.Wei-fen Li,Jian-yi Sun,Zi-xong Xu.Effects of NSP degrading enzyme on in vitro digestion of barley.Asian-Australasian Journal of Animal Sciences.2004,17(1):122-126. 70.李卫芬,郑佳佳,张小平,邓斌.反硝化酶及其环境影响因子的研究进展[J].水生生物学报,2014,01:166-170. 71.余东游,史艳云,邓斌,毛翔飞,林志伟,李卫芬.柔嫩艾美耳球虫3-1E基因工程活载体疫苗对肉鸡的保护效果[J].中国兽医学报,2014,03:431-435. 72.张小平,王一冰,邓斌,郑佳佳,胡彩虹,吴学祥,李卫芬.添加不同益生菌对草鱼养殖水体菌群结构的影响[J].水生生物学报,2014,03:459-466. 73.杜威,黄琴,付爱坤,余东游,李卫芬.解淀粉芽孢杆菌SC06对免疫抑制小鼠肠道细菌酶活性和肠黏膜屏障功能的影响[J].动物营养学报,2014,03:819-826. 74.李卫芬,白洁,李雅丽,秦艳,余东游.枯草芽孢杆菌对肉鸡肉品质、养分消化率及血清生化指标的影响[J].中国兽医学报,2014,10:1682-1685. 75.胡胜兰,李卫芬,孙建栋,林志伟,曹翠平,周绪霞.蜜蜂肽Apidaecin突变体的体外稳定性研究.中国兽医学报.2013.33(8):1259-1262. 76.郑佳佳,张小平,胡彩虹,李卫芬.斯氏假单胞菌对草鱼养殖水体水质的调控作用.渔业现代化,2013,03:5-9. 77.郑佳佳,彭丽莎,张小平,孙健栋,傅罗琴,王一冰,胡彩虹,李卫芬.复合益生菌对草鱼养殖水体水质和菌群结构的影响.水产学报,2013,37(003):457-464. 78.黄琴,白洁,汪启翰,黄怡,李雅丽,余东游,李卫芬.芽孢杆菌对体外肠上皮细胞黏附和细胞膜完整性的影响.中国兽医学报2013,33(003):376-380. 79.王一冰,张小平,黄怡,李卫芬.运用454焦磷酸测序技术对仔猪断奶前后肠道菌群的分析.动物营养学报.2013,25(10):2440-2446. 80.付爱坤,胡胜兰,杜威,史艳云,李卫芬.益生菌对巨噬细胞的免疫调节作用.动物营养学报.2013,25(12):2833-2837. 81.黄怡,黄琴,李卫芬*,余东游,周绪霞.鼠李糖乳杆菌对Caco-2细胞抗氧化功能和细胞因子分泌的影响.畜牧兽医学报,2012,43(2):250-254. 82.黄琴,黄怡,崔志文,李雅丽,李卫芬*,余东游.鼠李糖乳杆菌对巨噬细胞先天性免疫应答的调节作用.中国农业科学.2012,45(8):1621-1626. 83.黄怡,黄琴,李雅丽,崔志文,李卫芬*,余东游.乳酸菌对Caco-2细胞分泌APRIL和IL-10的影响.中国农业科学,2012,45(9):1881-1886. 84.李卫芬,张小平,宋文辉,邓斌,梁权,傅罗琴,郑佳佳,余东游.养殖水体中添加芽孢杆菌对草鱼免疫和抗氧化功能的影响.中国水产科学,2012,19(6):1027-1033. 85.李卫芬,张小平,宋文辉,胡彩虹,余东游.芽孢杆菌对草鱼养殖水质的影响.水产养殖,2012,33(10):1-5. 86.李卫芬,邓斌,陈南南,梁权,周绪霞.芽孢杆菌对草鱼生长和肠粘膜抗氧化功能及养殖水质的影响.水生态学杂志,2012,33(1):65-70. 87.李卫芬;沈涛;陈南南;邓斌;傅罗琴;周绪霞.饲料中添加枯草芽孢杆菌对草鱼消化酶活性和肠道菌群的影响.大连海洋学院学报,2012,27(3):221-225. 88.李卫芬,朱海燕,余东游,傅罗琴,李梅,高研.姜中其F18菌毛粘附素F基因(fedF)在大肠杆菌中的表达及对小鼠的免疫保护效果.农业生物技术学报,2012,20(9):1065-1071. 89.李卫芬,李雅丽,秦艳,余东游.枯草芽孢杆菌对肉鸡肠道消化酶活性、黏膜结构及肠道菌群组成的影响.中国兽医学报,2012,32(5):666-669. 90.毛予龙,徐歆,胡胜兰,郑建林,李雅丽,李卫芬*.甘草酸苷对体外小鼠单核巨噬细胞细胞因子分泌的影响,中国预防兽医学报,2012,10:823-826. 91.徐歆,毛予龙,李雅丽,胡胜兰,吴红照,李卫芬*.枯草芽孢杆菌B1对饲喂高脂饲粮小鼠抗氧化功能的影响,动物营养学报,2012,24(10):2067-2072. 92.傅罗琴,孙健栋,邓斌,梁权,张小平,郑佳佳,胡彩虹,李卫芬*.草鱼养殖水体中参与氮转化途径的异养菌分析.水产学报,2012,36(10):1572-1578. 93.傅罗琴,邓斌,李梅,沈文英,梁权,李卫芬*.嗜水气单胞菌外膜蛋白(OMP)在乳酸乳球菌中的表达及其对BALB/c小鼠的免疫保护效果.农业生物技术学报,2012,20(4):436-442. 94.傅罗琴,邓斌,郑佳佳,张小平,梁权,李卫芬*.一株产生氨氮和亚硝酸盐氮菌的筛选鉴定及特性研究.农业环境科学学报,2012,31(8):1616-1623. 95.黄怡,黄琴,崔志文,李卫芬*,余东游.新生仔猪口服屎肠球菌对其肠道菌群组成及化学屏障的影响.中国兽医学报,2012,32(7):1007-1010,1019. 96.黄怡,黄琴,李卫芬*,余东游,周绪霞.鼠李糖乳杆菌对Caco-2细胞抗氧化功能和细胞因子分泌的影响.畜牧兽医学报,2012,43(2):250-254. 97.黄琴,黄怡,崔志文,李雅丽,李卫芬*,余东游.鼠李糖乳杆菌对巨噬细胞先天性免疫应答的调节作用.中国农业科学.2012,45(8):1621-1626. 98.黄怡,黄琴,李雅丽,崔志文,李卫芬*,余东游.乳酸菌对Caco-2细胞分泌APRIL和IL-10的影响.中国农业科学,2012,45(9):1881-1886. 99.卞国顺,崔志文,姚庞,李卫芬*.枯草芽孢杆菌替代抗生素对肉鸡生产性能和肠道黏膜酶活的影响.饲料工业.2012,33(9):34-36. 100.文静,林志伟,周绪霞,吴红照,崔志文,李卫芬*.枯草芽孢杆菌B10对饲喂高脂日粮小鼠抗氧化功能的影响.中国兽医学报,2012,32(1):111-114. 101.沈文英,傅玲琳,李卫芬*.枯草芽孢杆菌表达的VP28对南美白对虾免疫力和抗病毒感染的影响.水生生物学报,2012,36(2):375-378. 102.沈涛,刘斯开,傅罗琴,郑佳佳,李卫芬*.光合细菌在鱼类养殖上的应用及其作用机理.水产科学,2012,31(2):115-118. 103.沈涛,邓斌,陈南南,傅罗琴,郑佳佳,李卫芬*.饲料中添加复合芽孢杆菌对草鱼消化道酶活性及肠道菌群的影响.淡水渔业,2012,42(1):41-45. 104.白洁,李卫芬*,黄琴,崔志文,余东游,黄怡.几株益生乳酸菌对Caco-2细胞的黏附及其对致病菌黏附的影响.动物营养学报,2012,24(10):1992-1998. 105.焦志华,徐娥,陆平,李卫芬*.4种芽孢杆菌来源木聚糖酶基因在大肠杆菌中的表达及酶活分析.饲料工业,2012,33(16):50-54. 106.文静,黄琴,吴红照,黄怡,李卫芬*.12株芽孢杆菌体外抗氧化特性比较研究.中国畜牧杂志,2012,48(17):70-73. 107.郑佳佳,沈涛,傅罗琴,邓斌,李卫芬*.一株硝化反硝化菌的筛选鉴定及反硝化特性研究.水生生物学报,2012,36(1):1-7. 108.徐歆,毛予龙,黄琴,吴红照,崔志文,李卫芬*.特丁基对苯二酚对高脂日粮小鼠抗氧化功能的影响,营养学报,2012,34(1):89-91. 109.朱海燕,李卫芬*.壳聚糖对水产动物免疫性能的影响研究进展.饲料广角,2012,21:43-44. 110.李卫芬,傅罗琴,邓斌,陈南南,周绪霞.1株好氧反硝化菌的分离鉴定及反硝化特性研究.环境科学,2011,3(8):2403-2408. 111.李卫芬,张小平,宋文辉,邓斌,梁权,傅罗琴,郑佳佳,余东游*.芽孢杆菌对草鱼养殖水质调控作用的研究.渔业现代化,2011,38(4):22-26. 112.李卫芬,文静,吴红照,余东游*.枯草芽孢杆菌对肉鸡生长性能和肠黏膜抗氧化及免疫功能的影响.中国畜牧杂志,2011,47(9):58-61. 113.崔志文,黄琴,黄怡,吴红照,文静,李卫芬*.枯草芽孢杆菌对Caco-2细胞抗氧化功能的影响研究.动物营养学报.2011,23(2):293-298. 114.文静,孙建安,周绪霞,李卫芬*.屎肠球菌对仔猪生长性能、免疫和抗氧化功能的影响.浙江农业学报,2011,23(1):70-73. 115.李雅丽,秦艳,周绪霞,李卫芬*.6株芽孢杆菌的生物学特性比较研究.中国畜牧兽医.2011,38(4):62-65. 116.沈文英,李卫芬*,梁权,邓斌,陈南南,周绪霞.饲料中添加枯草芽孢杆菌对草鱼生长性能、免疫和抗氧化功能的影响.动物营养学报,2011,23(5):881-886. 117.崔志文,李卫芬*,黄琴,黄怡,吴红照,文静.鼠李糖乳杆菌对Caco-2细胞抗氧化功能的影响.中国农业科学,2011,44(23):4926-4932. 118.徐歆,黄琴,阎永贞,周绪霞,陈有亮,李卫芬*.西式火腿肠复配防腐剂的研制.食品科学,2011,32(14):356-360. 119.陈南南,徐歆,商丰才,黄琴,陈有亮,李卫芬*.不同防腐剂对3种模式腐败菌抑菌效果的比较.食品科学,2011,32(1):14-18. 120.胡彩虹,朱康,钱仲仓,栾兆双,李卫芬.硅胶控释型丁酸对断奶仔猪生长性能和肠黏膜屏障的影响.动物营养学报,2011,23(12):2170-2176. 121.余东游,毛翔飞,秦艳,李卫芬*.枯草芽孢杆菌对肉鸡生长性能及其抗氧化和免疫功能的影响.中国畜牧杂志.2010,46(3):22-25. 122.徐歆,余东游,李卫芬*.I型干扰素诱生机理的研究进展.中国预防兽医学报.2010,10:824-828. 123.翟玲,李卫芬,余东游*.枯草芽孢杆菌对肉鸡生长性能和抗氧化力的影响.中国兽医学报.2010,30(6):753-755,761. 124.余东游,周斌,毛翔飞,刘士杰,林志伟,李卫芬*.癸氧喹酯抗鸡柔嫩艾美耳球虫病机理研究.中国畜牧杂志,2010(11):62-64. 125.胡春霞,李梅,李卫芬*,许梓荣,沈文英.嗜水气单胞菌外膜蛋白(OMP)的原核表达及其对BALB/c小鼠的免疫保护研究.农业生物技术学报,2010(2):218-224. 126.沈文英,李卫芬*,高研.中华鳖病原性维隆气单胞菌的分离及药敏试验.水生生物学报,2010,34(5):1-7. 127.余东游,宋文辉,毛翔飞,林志伟,刘士杰,李卫芬*.柔嫩艾美耳球虫对肉鸡生长性能及免疫性能的影响.中国兽医学报,2010,30(11):1468-1471. 128.沈文英,李卫芬*,雷凯,吴兵兵,祝尧荣.黄颡鱼抗菌肽Hepcidin基因的克隆和表达分析.农业生物技术学报.2009,17(6):972-978. 129.沈文英,余东游,李卫芬*,罗文,祝尧荣.地衣芽孢杆菌对三角帆蚌消化酶活性、免疫指标和抗氧化指标的影响.动物营养学报.2009,21(1):95-100. 130.胡春霞,陆平,李卫芬*,许梓荣.短芽孢杆菌耐碱性木聚糖酶(xylB)的分子生物学研究.食品与生物技术学报,2009,28(1):86-90. 131.王敏奇,许晓玲,李卫芬*,许梓荣,马玉龙.一株红霉素降解菌红圆酵母的选育及其降解特性.农业生物技术学报,2009,17(2):341-346.

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